G. JANSSON
STUDIES ON THE METHODOLOGY FOR CHEMICAL SCREENING OF PROTEIN QUALITY IN CROP SEEDS [Abstract]
R. SIMIĆ and M. DENIĆ
RELATIONSHIP BETWEEN PROTEIN COMPOSITION AND THE CONTENT OF FREE AMINO ACIDS IN THE ENDOSPERM OF A NORMAL GENOTYPE AND AN OPAQUE-2 MUTANT OF MAIZE [Abstract]
B.K. MUKHERJEE, R.D. SINGH and RAJPAL SINGH
STUDIES ON MORPHOLOGICAL AFFINITIES BETWEEN INDIAN AND EXOTIC MAIZE GERMPLASM [Abstract]
D. HRANUELP and Božanka HRANUELP
CHARACTERIZATION AND MAPPING OF SIXTEEN NEWLY ISOLATED ASPOROGENOUS MUTANTS [Abstract]
M. VELISAVLJEV, M. MILETIĆ, Ljiljana NIKOLIĆ, Ž. GAVRILOVIĆ, P. MIKIĆ, Zorica PRODANOVIĆ, Olga ĆURČIĆ, Danica OBRADOVIĆ, Ruža KLEUT - JELIĆ, Jesenka STANKOVIĆ, D. VUKOVIĆ, M. STANULOVIĆ, Branislava MIJATOVIĆ, V. BAŠIČEVIĆ, Branka BOGDANOV, Milica BANIĆ, V. KIKINĐANIN, Vera NIKOLIĆ - DOVAT and A. KRSTIĆ
HEREDITARY AND CONGENITAL MALFORMATIONS IN THE REGION OF SOUTH BAČKA IN THE PERIOD 1968-1972 [Abstract]
D. EFINSKI and Milka ŠOPOVA
CONTRIBUTION TO KNOWLEDGE ABOUT THE VARIABILITY OF DERMATOGLYPHIC PATTERNS IN MALES WITH 47, XYY [Abstract]
Tatjana MATEJIĆ
THE INFLUENCE OF GENETIC FACTORS ON THE DETERMINATION OF THE DEVELOPMENT OF THE ERYTHROCYT AND MYELOID BLOOD CELL LINE IN THE BONE MARROW OF SOME MICE STRAIN [Abstract]
D. MARINKOVIĆ
BALANCING SELECTIONS AND THE MAINTAINANCE OF GENETIC POLYMORPHISMS [Abstract]
Published in „GENETIKA“ Vol. 7, No.1 (1975), pp. 1-23
© 1975 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
STUDIES ON THE METHODOLOGY FOR CHEMICAL SCREENING OF PROTEIN QUALITY IN CROP SEEDS
G. JANSSON
Laboratories of the International Atomic Energy Agency Seibersdorf, Austria
Abstract
Jansson, G. (1975): Studies on the methodology for chemical screening of protein quality in crop seeds. - Genetika, Vol. 7, No. 1, 1-23.
It is shown that lysine can be estimated in unhydrolyzed seed flour simply by shaking it in a buffered solution of 2.4.6--trinitrobenzenesulfonic acid (TNBS). Satisfactory correlations (r = 0.9) have been found between TNBS colour formation and lysine content in series of wheat and barley samples.
By using a modified Adamkievicz-Hopkin-Cole reagent tryptophan has been determined in unhydrolyzed wheat meal. The results indicate an average of found tryptophan of 95% with a standard deviation of 1-2%. Carrying out the analysis on enzymic hydrolysates of the same seed samples, the recovery of tryptophan was found to be 85% with a standard deviation of around 4%.
Model experiments with pure amino acids showed that iodoacetic acid alkylates methionine and cysteine at pH below 3 specifically. By using carbon-14 labelled reagent good correlations were obtained between incorporation of label into the seed flour and methionine content (r = 0.73 - 0.97 for various seed materials).
Lysine has been determined by enzymic decarboxylation using a special technique for the assay of evolved carbon dioxide. This was achieved by absorbing the gas in a sodium carbonated solution containing an acid-base indicator. The amount oi Co," liberated during the reaction was measured by the change in optical density of the gas absorbing medium. A reaction vessel is described in which the enzymic reaction can proceed and subsequently be assayed without interference from atmospheric carbon dioxide. The relative standard deviation in replicated lysine analyses was around 5% at a lysine concentration of 34 mM.
Published in „GENETIKA“ Vol. 7, No.1 (1975), pp. 25-40
© 1975 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
RELATIONSHIP BETWEEN PROTEIN COMPOSITION AND THE CONTENT OF FREE AMINO ACIDS IN THE ENDOSPERM OF A NORMAL GENOTYPE AND AN OPAQUE-2 MUTANT OF MAIZE
R. SIMIĆ and M. DENIĆ
Department of Genetics, INEP, 11080 Zemun, Yugoslavia
Abstract
Simić, R. and Denić, M. (1974): Relationship between protein composition and the content of free amino acids in the endosperm of normal genotype and an opaque-2 mutant of maize.- Genetika, Vol. 7, No. 1, 25-40.
Using an Osborn-Mendel procedure modified by introducing 2-mercaptoethanol, the accumulation of different protein fractions was investigated during the development of the endosperm from an opaque-2 mutant and a normal genotype of maize.. The data obtained showed that 22 days after pollination there was a higher accumulation of zein-2 in the normal genotype than in the mutant. However, the absolute amount of glu-telin in the mutant endosperm was higher than in the normal genotype. The amounts of other protein fractions in these two genotypes were similar to those reported earlier in the literature.
Analysis of a-amino nitrogen showed a higher amount of all 19 free amino acids studied per mutant endosperm than in the endosperm of normal maize. However, there were no such differences in relative concentrations for most of the free amino acids of both genotypes. This would suggest that the differences in the absolute amounts of free amino acids between the genotypes are a secondary effect of the opaque-2 gene action.
Published in „GENETIKA“ Vol. 7, No.1 (1975), pp. 41-46
© 1975 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
STUDIES ON MORPHOLOGICAL AFFINITIES BETWEEN INDIAN AND EXOTIC MAIZE GERMPLASM
B.K. MUKHERJEE, R.D. SINGH and RAJPAL SINGH
Division of Genetics Indian Agricultural Research Institute, New Delhi, India
Abstract
Mukherjee, B. K., Singh, R. D. and Singh, R. (1975): Studies on morphological affinities between Indian and exotic maize germplasm. - Genetika, Vol. 7, No. 1, 41-46.
The study relates to the morphological relationship of the maize types cultivated in the Indo-Gangetic plains with germplasm from Mexico and Colombia. In an earlier study, Mukherjee et a/. (1971) reported that Indian germplasm in general, resembled the Mexican and Colombian races of maize but there existed one group of varieties which bore greater resemblance to the Caribbean maize types. This group included the varieties grown in the southern part of the country. This study, however, did not include a large number of varieties so as to represent fully a particular zone of the country. In the present investigation a large number of varieties from the North-Eastern and North-Western plains which represents the corn belt of the country and also possess the maximum variability in the maize types grown, were included. Thirty two varieties belonging to different races of maize from Mexico and Colombia and 32 distinct local Indian types were laid out in a randomised block design with four replications. A number of morphological traits were recorded and metroglyph and index score analysis was carried out following Anderson (1957). It was found that on a maturity basis, the Indian varieties resolved into two distinct groups. The early group was different from the medium to late group which showed greater affinities with the exotic group. The early group appears to consist of variants of some introduction other than Mexican-Colombian.
Published in „GENETIKA“ Vol. 7, No.1 (1975), pp. 47-60
© 1975 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
CHARACTERIZATION AND MAPPING OF SIXTEEN NEWLY ISOLATED ASPOROGENOUS MUTANTS
D. HRANUELP and Božanka HRANUELP
Microbiology Unit, Department of Biochemistry, University of Oxford, England
Abstract
Hranueli, D. and Hranueli, B. (1975): Characterization and mapping of sixteen newly isolated asporogenous mutants. - Genetika, Vol. 7, No. 1, 47-60.
Sixteen asporogenous mutants were characterized in order to establish their morphological and biochemical phenotypes. Transduction crosses have shown that the isolated mutants were mapped in four regions on the Bacillus subtilis chromosome.
Published in „GENETIKA“ Vol. 7, No.1 (1975), pp. 61-76
© 1975 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
HEREDITARY AND CONGENITAL MALFORMATIONS IN THE REGION OF SOUTH BAČKA IN THE PERIOD 1968-1972
M. VELISAVLJEV, M. MILETIĆ, Ljiljana NIKOLIĆ, Ž. GAVRILOVIĆ, P. MIKIĆ, Zorica PRODANOVIĆ, Olga ĆURČIĆ, Danica OBRADOVIĆ, Ruža KLEUT - JELIĆ, Jesenka STANKOVIĆ, D. VUKOVIĆ, M. STANULOVIĆ, Branislava MIJATOVIĆ, V. BAŠIČEVIĆ, Branka BOGDANOV, Milica BANIĆ, V. KIKINĐANIN, Vera NIKOLIĆ - DOVAT and A. KRSTIĆ
Institut za zdravstvenu zaštitu majke i deteta, 2100 Novi Sad, Jugoslavija
Abstract
Velisavljev, M., Miletić, D., Nikolić, Lj., Gavrilović, P., Mikic, P., Prodanović, Z., Ćurčić, O., Obradović, D., Kleut - Jelić, R., Stanković, J., Vuković, D., Stanulović, M., Mijatović, B., Bašićević, V., Bogdanov, B., Banić, M., Kikinđanin, V., Nikolić - Dovat, V. and Krstić, A. (1975): Hereditary and congenital malformations in the region of South Backa in the period 1968-1972.- Genetika, Vol. 7, No. 1, 61-76.
The frequency and distribution of hereditary and congenital malformations were analysed at the Department of Obstetrics and Gynaecology of the University Teaching Hospital in Novi Sad in the period from 1968 do 1972. In a total of 19.391 newborn infants, among whom 1.436 were premature, 487 malformations have been found. The malformations were classified to be hereditary or congenital on the basis of clinical studies and a family inquiry.
Published in „GENETIKA“ Vol. 7, No.1 (1975), pp. 77-84
© 1975 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
CONTRIBUTION TO KNOWLEDGE ABOUT THE VARIABILITY OF DERMATOGLYPHIC PATTERNS IN MALES WITH 47, XYY
D. EFINSKI and Milka ŠOPOVA
Rehabilitating Center for Mental Retardation, 91000 Skopje, Yugoslavia
Abstract
Efinski, D. and Šopova, M. (1975): Contribution to knowledge about the variability of dermatoglyphic patterns in males with 47, XYY, - Genetika, Vol. 7, No. 1, 77-84.
The present paper reports an investigation of dermatoglyphs in three patients with the chromosome constitution 47, XYY. Analysis of the dermatoglyphs revealed that a great variability of dermatoglyphic model existed among these patients. Thus the data obtained in the present study confirms the findings of other authors that the extra Y chromosome does not significantly affect the dermatoglyphic patterns of 47, XYY patients.
Published in „GENETIKA“ Vol. 7, No.1 (1975), pp. 85-94
© 1975 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
THE INFLUENCE OF GENETIC FACTORS ON THE DETERMINATION OF THE DEVELOPMENT OF THE ERYTHROCYT AND MYELOID BLOOD CELL LINE IN THE BONE MARROW OF SOME MICE STRAIN
Tatjana MATEJIĆ
Institut za medicinska istraživanja, 11000 Beograd, Jugoslavija
Laboratorija za eksperimentalnu hematologiju
Abstract
Matejić, T. (1975): The Influence of genetic factors on the determination of the development of the erythrocyt and myeloid Mood cell line in the. bone marrow of some mice strain. - Genetika, Vol. 7, No. 1, 85-94.
There are differences between laboratory white mouse (Mus musculus albinos) and black mouse C57BL strain, in the absolute and relative cell number of erythroid and myeloid cell lines in the bone marrow. An experiment is undertaken to determine the eventual influence of the genetic control factors on these differences. The matting between white mouse female and C57BL male and a microscopic analysis of the bone marrow mentioned mouse strains and their hybrids has been done.
The hybrids show positive or negative heterotic effect in respect to their parents. A significant correlation between the blood lines in the genetically connected groups shows the connection in the genetic determination of these lines. The heritabilites of the relative and absolute cell number of erythroid and myeloid lines are given.
Published in „GENETIKA“ Vol. 7, No.1 (1975), pp. 95-107
© 1975 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
BALANCING SELECTIONS AND THE MAINTAINANCE OF GENETIC POLYMORPHISMS
D. MARINKOVIĆ
Faculty of Science and Institute for Biological Research, Belgrade, Yugoslavia
Abstract
Marinković, D. (1975): Balancing selections and the maintenance of genetic polymorphisms. - Genetika, Vol. 7, No. 1, 95-107.
This paper is a review of some recent studies which attempt to explain the adaptive significance of gene mutations in natural populations of living organisms. In two of these papers it has been shown that in Drosophila pseudoobscura allozyme variants coded by different alleles at five loci (Pgm-1 and Est-5 in the X-chromosome, Me-2 and Odh in the second chromosome, and Mdh-2 in the fourth chromosome) affect a variety of fitness components, and thus are not adaptively neutral (Marinković and Ayala, 1975a, 1975b). Several kinds of balancing selection have been uncovered by these experiments, which may account for the persistence of the allozyme polymorphisms in five studied loci in nature, and for previously observed seasonal fluctuations of the allelic frequencies at Pgm-1 and Me-2 loci (Dobzhansky and Ayala, 1973). Our present experiment provides some more information about the means of selection effects on differential survival of allozyme variants at Est-5, Odh and Mdh-2 loci, in a number of successive generations. It may suggest some basic mechanisms by which selection regulates allelic frequencies in a natural population in the long term, especially in fluctuating environmental conditions.