GENETIKA, Vol. 45, No. 3 (2013)

Valentina DJORDJEVIC, Aleksandra RANKOV DIVAC, Marija STANKOVIC, Vesna SRECKOVIC BRANKOVIC, Dragica RADOJKOVIC

TUMOR NECROSIS FACTOR ΑLPHA AND ALPHA-1 ANTITRYPSIN GENE VARIANTS IN SERBIAN PEDIATRIC ARTERIAL ISCHEMIC STROKE PATIENTS [Abstract] [Full text]

Kairat URAZALIEV, Alfiya ABEKOVA, Tamara BAZYLOVA, Gulnara BERSIMBAEVA, Aliya DANIYAROVA, Raushan MASSONICHICH-SHOTUNOVA

SOMACLONAL VARIATION OF SUGAR BEET RESISTANT TO PATHOGENIC ROOT ROT Fusarium Oxysporum var. Orthoceras [Abstract] [Full text]

Aleksandar LUČIĆ, Rade CVJETIĆANIN, Marijana NOVAKOVIĆ-VUKOVIĆ, Danijela RISTIĆ, Vladan POPOVIĆ, Ljubinko RAKONJAC, Snežana MLADENOVIĆ DRINIĆ

INTERPOPULATION VARIABILITY OF AUSTRIAN PINE (Pinus nigra Arnold) IN SERBIA [Abstract] [Full text]

Ramanuj MAURYA, Saurabh VERMA, Astha GUPTA, Bajrang SINGH and Hemant KUMAR YADAV

GENETIC VARIABILITY AND DIVERGENCE ANALYSES IN Jatropha curcas BASED ON FLORAL AND YIELD TRAITS [Abstract] [Full text]

Živorad VIDENOVIĆ, Zoran DUMANOVIĆ, Milena SIMIĆ, Jelena SRDIĆ, Milosav BABIĆ, Vesna DRAGIČEVIĆ

GENETIC POTENTIAL AND MAIZE PRODUCTION IN SERBIA [Abstract] [Full text]

Jasmina ZDRAVKOVIĆ, Zorica JOVANOVIĆ, Mladen DJORDJEVIĆ,Zdenka GIREK, Milan ZDRAVKOVIĆ, Radmila STIKIĆ

Application of stress susceptibility index for drought tolerance screening of TOMATO POPULATIONS [Abstract] [Full text]

Naser SABAGHNIA,Mohtasham MOHAMMADI, Rahmatollah KARIMIZADEH

PRINCIPAL COORDINATE ANALYSIS OF GENOTYPE × ENVIRONMENT INTERACTION FOR GRAIN YIELD OF BREAD WHEAT IN THE SEMI-ARID REGIONS [Abstract] [Full text]

Gordana MINOVSKA, Tanja NARANČIĆ, Mina MANDIĆ, Lidija ŠENEROVIĆ, Branka VASILJEVIĆ and Jasmina NIKODINOVIĆ-RUNIĆ

LIMITED AROMATIC PATHWAY GENES DIVERSITY AMONGST AROMATIC COMPOUND DEGRADING SOIL BACTERIAL ISOLATES [Abstract] [Full text]

Ibrahim Kursat OZYURT, Yasar AKCA and Sezai ERCISLI

MOLECULAR CHARACTERIZATION OF Prunus mahaleb L. ROOTSTOCK CANDITATES BY ISSR MARKERS [Abstract] [Full text]

Slavica POPOVIĆ BUBUJUK, Nenad Č. BOJAT,Ninoslav DJELIĆ, Slađana DRONJAK, Ljiljana KOSTADINOVIĆ, Tamara COGHILL GALONJA, Marko ANDJELKOVIĆ

THE EFFECT OF DIFFERENT ACUTE CONCENTRATIONS OF CADMIUM CHLORIDE ON THE FREQUENCY OF MICRONUCLEI IN AO RATS [Abstract] [Full text]

Masoumeh AMOUZADEH, Reza DARVISHZADEH¹, Parham HADDADI, Babak ABDOLLAHIMANDOULAKANI, Younes REZAEE DANESH

GENETIC ANALYSIS OF PARTIAL RESISTANCE TO BASAL STEM ROT (Sclerotinia sclerotiorum) IN SUNFLOWER [Abstract] [Full text]

Tamara KOVAČEVIĆ, Jelena LEVIĆ, Slavica STANKOVIĆ,Jelena VUKOJEVIĆ

MATING POPULATIONS OF Gibberella fujikuroi (Sawada) S. ITO SPECIES COMPLEX ISOLATING FROM MAIZE, SORGHUM AND WHEAT IN SERBIA [Abstract] [Full text]

Rosaiah KOTIKALAPUDI, Rajesh Kumar PATEL, Hemanth MEDIDI and Nagaraju Naik SUGALI

DISTRIBUTION OF SEX CHROMOSOMES (XY) IN LYMPHOCYTE METAPHASE SPREADS OF DAIRY BULLS [Abstract] [Full text]

Milka BRDAR-JOKANOVIĆ, Dragana TRKULJA, Emilija NIKOLIĆ-ĐORIĆ, Ankica KONDIĆ-ŠPIKA, Borislav KOBILJSKI

THE POSSIBILITIES OF APPLYING MARKER ASSISTED SELECTION IN BREEDING BORON TOLERANT WHEAT [Abstract] [Full text]

Amir Ali KHALATBARI, Hawa Z.E. JAAFAR, Maziah MAHMOOD, Radziah OTHMAN, Amir Mahdi KHALATBARI

EFFECTS OF SALT STRESS ON WILD TYPE AND VTE₄MUTANT Arabidopsis thaliana: MODEL PLANT TO ENGINEER TOLERANCE TOWARDS SALINITY [Abstract] [Full text]

Jelena BLAGOJEVIĆ, Vladimir JOVANOVIĆ, Tanja ADNAĐEVIĆ, Ivana BUDINSKI and Mladen VUJOŠEVIĆ

CHROMOSOME STATUS OF MARSH MARIGOLD, Caltha palustris L. (Ranunculaceae) FROM SERBIA [Abstract] [Full text]

Abdulmojeed YAKUBU, Ibrahim Suleiman MUSA-AZARA, Blessing N. Saleh YAKUBU, Sylvester I. DAIKWO, Samuel Ter VINCENT, Ojo Michael MOMOH and Ndubuisi I. DIM

BIOCOMPUTATIONAL GENOME-WIDE ANALYSIS OF MICRO RNA GENETIC VARIABILITY IN SOME VERTEBRATES [Abstract] [Full text]

Danijela RISTIĆ, Vojka BABIĆ, Violeta ANĐELKOVIĆ, Jelena VANČETOVIĆ, Snežana MLADENOVIĆ DRINIĆ, Dragana IGNJATOVIĆ-MICIĆ

GENETIC DIVERSITY IN MAIZE DENT LANDRACES ASSESSED BY MORPHOLOGICAL AND MOLECULAR MARKERS [Abstract] [Full text]

Mária LABAJOVÁ, Jana ŽIAROVSKÁ, Katarína RAŽNÁ, Jaroslava OVESNÁ, Andrea HRICOVÁ

USING OF AFLP TO EVALUATE GAMMA-IRRADIATED AMARANTH MUTANTS [Abstract] [Full text]

Imen REKIK, Amine ELLEUCH, Walid KRIAA, Noureddine DRIRA

MOLECULAR CLONING AND IN SILICO ANALYSIS OF THREE SOMATIC EMBRYOGENESIS RECEPTOR KINASE mRNA FROM DATE PALM [Abstract] [Full text]

Hamid HATAMI MALEKI, Ghasem KARIMZADEH, Reza DARVISHZADEH, Mohammad Reza NAGHAVI and Ahmad SARRAFI

IDENTIFICATION OF QTLS ASSOCIATED WITH LOW CHLORIDE ACCUMULATION IN ORIENTAL TOBACCO [Abstract] [Full text]

Nela MAKSIMOVIĆ, Ivana NOVAKOVIĆ, Vesna RALIĆ and Elka STEFANOVA

DISTRIBUTION OF Apolipoprotein E gene polymorphism in students and in high-educated elderly from Serbia [Abstract] [Full text]

Milica FOTIRIĆ AKŠIĆ and Tihomir NIKOLIĆ

ANALYSIS OF POMOLOGICAL TRAITS IN NEW PROMISING SWEET CHERRY GENOTYPES [Abstract] [Full text]

Rajeev Kumar PANDEY, Abid ALI, Minu BAJPAI, Sukanya GAYAN, Amit SINGH

ASSOCIATION STUDY OF FIVE MUTATION IN FGFR1 AND FGFR2 GENES IN INDIAN CHILDREN WITH CRANIOSYNOSTOSIS [Abstract] [Full text]

Dalibor BALLIAN, Vasilije ISAJEV, Vanja DANIČIĆ, Branislav CVJETKOVIĆ, Faruk BOGUNIĆ, Milan MATARUGA

GENETIC DIFFERENTIATION IN SEED STANDS OF EUROPEAN BEECH (Fagus sylvatica L.) IN PART OF BOSNIA AND HERZEGOVINA [Abstract] [Full text]

Vesna DRAGIČEVIĆ, Snežana MLADENOVIĆ DRINIĆ, Milovan STOJILJKOVIĆ, Milomir FILIPOVIĆ, Zoran DUMANOVIĆ, Dragan KOVAČEVIĆ

VARIABILITY OF FACTORS THAT AFFECT AVAILABILITY OF IRON, MANGANESE AND ZINC IN MAIZE LINES [Abstract] [Full text]

Miroslav BUKAN, Hrvoje ŠARČEVIĆ, Ivica BUHINIČEK, Branko PALAVERŠIĆ, Ramsey S. LEWIS, Vinko KOZUMPLIK

STALK ROT RESISTANCE IN MAKSIMIR 3 SYNTHETIC MAIZE POPULATION AFTER FOUR CYCLES OF RECURRENT SELECTION [Abstract] [Full text]

Predrag KALAJDZIC, Stefan OEHLER, Maria MARKAKI, Charalambos SAVAKIS

TOXICOLOGY AND CYTOGENETIC ANALYSIS OF A Drosophila melanogaster MUTANT RESISTANT TO IMIDACLOPRID AND DDT [Abstract] [Full text]

Sanja RADIČEVIĆ, Slađana MARIĆ, Radosav CEROVIĆ, Milena ĐORĐEVIĆ

ASSESSMENT OF SELF-(IN)COMPATIBILITY IN SOMESWEET CHERRY (Prunus avium L.) GENOTYPES [Abstract] [Full text]

Veljko GAVRILOVIĆ, Žarko IVANOVIĆ, Tatjana POPOVIĆ, Svetlana ŽIVKOVIĆ, Slaviša STANKOVIĆ, Tanja BERIĆ and Đorđe FIRA

GENETIC CHARACTERIZATION OF PATHOGENIC FLUORESCENT PSEUDOMONADS ISOLATED FROM NECROTIC CHERRY AND PLUM BUDS IN SERBIA [Abstract] [Full text]

Jasmin GRAHIĆ, Fuad GAŠI, Mirsad KURTOVIĆ, Lutvija KARIĆ, Mirha ĐIKIĆ, Drena GADŽO

MORPHOLOGICAL EVALUATION OF COMMON BEAN DIVERSITY IN BOSNIA AND HERZEGOVINA USING THE DISCRIMINANT ANALYSIS OF PRINCIPAL COMPONENTS (DAPC) MULTIVARIATE METHOD [Abstract] [Full text]

Published in „GENETIKA“ Vol. 45, No. 3 (2013), pp. 621-628

Serbia

UDC 575
DOI: 10.2298/GENSR1303621D

Original scientific paper

TUMOR NECROSIS FACTOR ΑLPHA AND ALPHA-1 ANTITRYPSIN GENE VARIANTS IN SERBIAN PEDIATRIC ARTERIAL ISCHEMIC STROKE PATIENTS

Valentina DJORDJEVIC¹, Aleksandra RANKOV DIVAC¹, Marija STANKOVIC¹, Vesna SRECKOVIC BRANKOVIC², Dragica RADOJKOVIC¹

¹Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia

² Clinic for Child Neurology and Psychiatry, School of Medicine, University of Belgrade, Belgrade, Serbia

Abstract

Djordjevic V., A. Rankov Divac, M. Stankovic, V. Sreckovic Brankovic, and D. Radojkovic (2013): Tumor necrosis factor αlpha and alpha-1 antitrypsin gene variants in Serbian pediatric arterial ischemic stroke patients- Genetika, vol., No.45, No.3, 621-628.

The etiology of arterial ischemic stroke (AIS) in children is complex, and different from that in adults. Although rare, stroke in children is an important cause of mortality and morbidity. There is increasing evidence that genetic factors, including inflammation mediators, have a role in occurrence and outcome of stroke. We have chosen to assess the role of polymorphism -308G/A in the promoter of tumor necrosis factor α (TNFα) gene and S and Z mutations in alpha 1-antitrypsin (AAT) gene in the etiology of stroke in children. TNFα polymorphism affects plasma levels of this proinflamatory cytokine, and this could contribute to stroke pathology. It has been shown that increased AAT concentration may present a risk for AIS in children. Since S and Z mutations in AAT gene reduce its levels in plasma they could have a protective role in pediatric stroke.In this study twenty six children with AIS and 100 unrelated individuals from Serbian general population were investigated by PCR/RFLP for these gene variations.No statistically significant difference was observed between patients and general population in distribution of genotypes for -308G/A TNFα polymorphism, so its contributory role in the etiology of stroke was not evident in our group of patients.None of the tested AAT gene mutations were found in patients, which is in concordance with the proposed protective role of deficient AAT variants.AIS is a multifactorial disease, with many genes having a modest role in its pathophysiology, so further analyses of their combined effect are needed to elucidate genetic risk factors in the etiology and outcome of stroke in pediatric patients.

Key words: tumor necrosis factor α (TNFα), alpha 1-antitrypsin, arterial ischemic stroke, children

Corresponding author: Aleksandra Divac Rankov,,Institute of Molecular Genetics and Genetic EngineeringVojvode Stepe 444°,PO Box 23,11010 elgrade,Serbia,Tel: +381 11 3976658

Fax: +381 11 3975808,E-mail: aleksandradivac@imgge.bg.ac.rs

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Published in „GENETIKA“ Vol. 45, No. 3 (2013), pp. 629 -640

Serbia

UDC 575
DOI:10.2298/GENSR1303629U

Original scientific paper

SOMACLONAL VARIATION OF SUGAR BEET RESISTANT TO PATHOGENIC ROOT ROT Fusarium Oxysporum var. Orthoceras

Kairat URAZALIEV, Alfiya ABEKOVA, Tamara BAZYLOVA, Gulnara BERSIMBAEVA, Aliya DANIYAROVA, Raushan MASSONICHICH-SHOTUNOVA

Laboratory of Biotechnology, Kazakh Research Institute of Agriculture and Crop Production,Almalybak, Karasay district, Almaty region, Republic of Kazakhstan

Abstract

Urazaliev K., A. Abekova, T. Bazylova, G. Bersimbaeva, A. Daniyarova, R. Massonichich-Shotunova (2013): Somaclonal variation of sugar beet resistant to pathogenic root rot Fusarium oxysporum var. orthoceras.Genetika, Vol 45, No. 3, 629-640.

Sugar beet (Beta vulgaris L.) – one of the most important crop in the world. In Kazakhstan, it is a traditional and major source of domestic sugar. The industry of cultivation and production of sugar beet is one of the priority areas of agricultural development of the country. In this paper, we studied the regeneration ability of different genotypes of sugar beet explants on selective media with the culture filtrate of the pathogen fungus F. oxysporum var. orthoceras. From the roots and shoots of sugar beet the pathogen Fusarium root rot was isolated. Was obtained pure cultures of the isolated pathogen. As a result, of morphological and cultural descriptions, as well as microbiological analysis it was revealed that the isolated pathogen is Fusarium Oxysporum. The results showed the pathogenicity of the fungus. For regeneration in vitro of the sugar beet genotypes resistant to the pathogen the culture media was optimized to the culture filtrate of the fungus F. oxysporum var. orthoceras. The frequency of shoot regeneration, depending on the genotype, was 1,0–12,5 %. On these explants the multiple shoot formations were observed.

Key words: sugar beet, pathogen, fusarium, root rot, culture filtrate

Corresponding author: Urazaliev Kairat, Laboratory of Biotechnology, Kazakh Research Institute of Agriculture and Crop Production, 040909, Erlepesova Street 1, v. Almalybak, Karasay district, Almaty region, Republic of Kazakhstan, Phone: +7 72771 53 130. E-mail: kairatu@mail.ru.

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Published in „GENETIKA“ Vol. 45, No. 3 (2013), pp. 641- 654

Serbia

UDC 575:633
DOI: 10.2298/GENSR1303641L

Original scientific paper

INTERPOPULATION VARIABILITY OF AUSTRIAN PINE (Pinus nigra Arnold)

IN SERBIA

Aleksandar LUČIĆ¹, Rade CVJETIĆANIN², Marijana NOVAKOVIĆ-VUKOVIĆ², Danijela RISTIĆ³, Vladan POPOVIĆ¹, Ljubinko RAKONJAC¹, Snežana MLADENOVIĆ DRINIĆ³

¹Institute of Forestry, Belgrade,Serbia

²University of Belgrade, Faculty of Forestry,Belgrade, Serbia

³Maize Research Institute “ Zemun Polje”, Belgrade, Serbia

Abstract

Lučić A., R. Cvetičanin, M. Novaković-Vuković, D. Ristić, V. Popović, LJ.Rakonjac, and S. Mladenović Drinić (2013): Interpopulation variability of Austrian pine (Pinus nigra Arnold) in Serbia.-Genetika, Vol 45, No. 3, 641-654.

Multidisciplinary studies (genetic and phytocoenological) of 4 populations of Austrian pine (Pinus nigra Arnold) were carried out in western and central Serbia. The obtained results gave the same inter-population arrangement in both methods. Dendograms (NTSYS) differentiate at the greatest genetic distance the population of Austrian pine in Šargan compared to other populations: Crni Vrh, Goč and Studenica. Using phytocoenological analysis it was determined that population of Austrian pine in Šargan forms a community Erico-Pinetum gocensis, while the other populations form a community Seslerio rigidae-Pinetum gocensis. Multidisciplinary approach that was demonstrated within this paper presents the first studies of Austrian pine that directly link genotype dependence and environmental conditions manifested through the phytocoenological affiliation.

Key words: Austrian pine, RAPD, plant communities.

Corresponding author: Aleksandar Lučić; Institute of Forestry; Kneza Višeslava 3; 11000 Belgrade; Serbia; Phone:011-3553355; e-mail:aleksandar.lucic@gmail.com

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Published in „GENETIKA“ Vol. 45, No.3 (2013), pp. 655 -666

Serbia

UDC 575
DOI: 10.2298/GENSR1303655M

Original scientific paper

GENETIC VARIABILITY AND DIVERGENCE ANALYSES IN Jatropha curcas

BASED ON FLORAL AND YIELD TRAITS

Ramanuj MAURYA¹, Saurabh VERMA¹, Astha GUPTA¹, Bajrang SINGH¹

and Hemant KUMAR YADAV^1,2*

¹CSIR-National Botanical Research Institute, Lucknow-226001, India.

²Academy of Scientific and Innovative Research (AcSIR), New Delhi, India

Abstract

Maurya R., S. Verma, A. Gupta, B.Singh, and H. Kumar Yadav (2013): Genetic variability and divergence analyses in Jatropha curcas based on floral and yield traits -. Genetika, Vol 45, No. 3, 655-666.

Genetic variability of 80 accessions of Jatropha curcas showed that oil content varied between 20.8-36.1% (X=26.2±0.38). Thirty seven accessions showed seed weight/plant above average mean value (180.2g) and 26 accessions showed oil content above average mean (26.2%). The hierarchical clustering grouped all the accessions into 4 clusters. Clustering showed that majority of accessions i.e. 56 (70%) were genetically close to each other and grouped in two clusters. The maximum intra cluster distance was recorded in cluster IV (30.15). The inter cluster distance varied from 47.59 (between cluster I and cluster II) to 211.27 (between cluster III and cluster I). The cluster III showed maximum genetic distance with cluster I, followed by cluster IV and cluster II suggesting comparatively wider genetic diversity among them. The Principal Component Analysis (PCA) showed that first four principal components (PCs) accounted for more than 93% of the total variation. The first principal components accounted for 42.5% of the total variation mainly due to seed length, seed width, seed weight/plant and number of seeds/plant which had maximum and positive weight on this component. Oil content had negative weight on PC1. Thus, PC1 related to the accessions with thick seeds, moderate to high seed yielder with low oil content.

Key words: Divergence, Genetic variability, Genetic advance, Heritability, Jatropha curcas

Corresponding author: Hemant Kumar Yadav,CSIR-National Botanical Research Institute, Lucknow-226001, India, Tel.: +91-522-2297982, Fax: +91-522-2205836, E-mail: h.yadav@nbri.res.in

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Published in „GENETIKA“ Vol. 45, No.32 (2013), pp. 667 -677

Serbia

UDC 575:633.15
DOI: 10.2298/GENSR130667V

Original scientific paper

GENETIC POTENTIAL AND MAIZE PRODUCTION IN SERBIA

Živorad VIDENOVIĆ, Zoran DUMANOVIĆ, Milena SIMIĆ, Jelena SRDIĆ,

Milosav BABIĆ, Vesna DRAGIČEVIĆ

Maize Research Institute, Zemun Polje, Belgrade – Zemun, Serbia

Abstract

Videnović Ž.., Z. Dumanović, M. Simić, J. Srdić, M.Babić and V. Dragičević (2013): Genetic potential and maize production in Serbia. Genetika, Vol 45, No. 3, 667-677.

Genetic potential of maize hybrids grown in Serbia is 10-15 t ha^-1, while the average yields are considerably lower. There are many reasons for this. At first, it is well known that drought is present often in some parts of country. Some soils are not suitable in the same degree for intensive maize production, application of mineral fertilizers is insufficient, mechanization is outdated and arable farms are small and fragmented. During the period 1965-2012 high variations in precipitation were present during the maize vegetation. The yearly average precipitation sum was 688.9 mm, with 397.5 mm during vegetation. According to precipitation amount, years were divided into groups: I 200-300 mm 7, (15%); II 301-400 mm 21, (44%) and III 401-500 mm 14, (30%), and IV in 5 years (11%) more than 500 mm of precipitation was present.The highest average yield in Serbia was achieved in 1991 (5.95 t ha^-1) and the lowest in 2000 (2.44 t ha^-1). The average yield increase was 114 kg ha^-1 per year from 1965 to 1985, and it was 22 kg ha^-1 per year from 1986 to 2012. In experiments during the period 1998-2012, when the standard cropping technology (MSY) was applied, the average grain yield was10.46 t ha^-1 for hybrids of FAO 300-400, 10.39 t ha^-1 for hybrids from FAO 500 and 11.38 for FAO 600-700. There were no significant differences in yield between hybrids from examined FAO groups. According to this, average maize yield includes only 44.2% for FAO 300-400, 44.5% for FAO 500 and 40.6% for FAO 600-700 utilized maize genetic potential.The significant improvement of maize production demand the strategic long-term program, where it will be elaborated: merging of land properties, increasing of the areas with irrigation and increasing of the technology level in maize cropping.

Key words: maize, yielding genetic potential, cropping technology, drought, yield trend.

Corresponding author: Živorad Videnović, Maize Research Institute „ZemunPolje“,S. Bajića 1, 11185 Belgrade, Serbia,e-mail: zvidenovic@mrizp.rs

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Published in „GENETIKA“ Vol. 45, No. 3 (2013), pp. 679 -689

Serbia

UDC 575:630
DOI: 10.2298/GENSR1303679Z

Original scientific paper

Application of stress susceptibility index for drought tolerance screening of TOMATO POPULATIONS

Jasmina ZDRAVKOVIĆ¹, Zorica JOVANOVIĆ², Mladen DJORDJEVIĆ¹,

Zdenka GIREK¹, Milan ZDRAVKOVIĆ¹, Radmila STIKIĆ²

¹Institute for Vegetable Crops, Smederevska Palanka, Serbia

²Faculty of Agriculture, University of Belgrade, Belgrade, Serbia

Abstract

Zdravković J., Z. Jovanović, M. Djordjević, Z. Girek, M. Zdravković and R. Stikić (2013): Application of stress susceptibility index for drought tolerance screening of tomato populations. Genetika, Vol 45, No. 3, 679-689.

Investigation comprised 41 tomato genotypes originating from the population of domestic and domesticated genotypes collected in Serbia and belonging to the tomato collection of the Institute of Vegetable Crop Science, Smederevska Palanka. The aim of collection screening was to choose the genotypes tolerant to drought during plant intensive growth stage, whereby the process of selection would set out to obtain the recombinant genotypes for this abiotic factor. The screening criteria were established for genotype divergence in plant height and shoot-root ratio under conditions of optimal irrigation regime and drought. Divergence was estimated using cluster analysis with Euclidean distance as a measure of distance, with a complete gene attachment to grouping. Drought tolerance is expressed by the stress susceptibility index (SSI). Various results were obtained based on the screening of genotypes grown under optimal and dry conditions. As a measure of stress susceptibility, based on SSI, genotypes having different drought tolerance level were determined. On the grounds of the analyses carried out, 10 genotypes were segregated (G102, G104, G107, G109, G110, G119, G125, G126, G128 and G141) to represent a basis to obtain the recombinant genotypes and to initiate the selection for drought resistance.

Key words: genetic divergence, stress susceptibility index, vegetative stage of growth, tomato

Corresponding author: Jasmina Zdravković, Institute for Vegetable Crops, Karadjordjeva 71, 11420 Smederevska Palanka, Serbia; tel.: +381 64/221-05-41; fax.: +381 26/317-785. e-mail: jzdravkovic@institut-palanka.co.rs

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Published in „GENETIKA“ Vol. 45, No. 3 (2013), pp. 691 -701

Serbia

UDC 575.633.11
DOI: 10.2298/GENSR1303691S

Original scientific paper

PRINCIPAL COORDINATE ANALYSIS OF GENOTYPE × ENVIRONMENT INTERACTION FOR GRAIN YIELD OF BREAD WHEAT IN THE SEMI-ARID REGIONS

Naser SABAGHNIA^1*,Mohtasham MOHAMMADI², Rahmatollah KARIMIZADEH²

¹Department of Agronomy and Plant Breeding, Faculty of Agriculture,

University of Maragheh, Maragheh, Iran.

²Dryland Agricultural Research Institute (DARI), Gachsaran, Iran

Abstract

Sabaghnia N., M. Mohammadi, and R. Karimizadeh (2013): Principal coordinate analysis of genotype × environment interaction for grain yield of bread wheat in the semi-arid regions. Genetika, Vol 45, No. 3, 691-701.

Multi-environmental trials have significant main effects and significant multiplicative genotype × environment (GE) interaction effect. Principal coordinate analysis (PCOA) offers a more appropriate statistical analysis to deal with such situations, compared to traditional statistical methods. Eighteen bread wheat genotypes were grown in four semi-arid regions over three year seasons to study the GE interaction and yield stability and obtained data on grain yield were analyzed using PCOA. Combined analysis of variance indicated that all of the studied effects including the main effects of genotype and environments as well as the GE interaction were highly significant. According to grand means and total mean yield, test environments were grouped to two main groups as high mean yield (H) and low mean yield (L). There were five H test environments and six L test environments which analyzed in the sequential cycles. For each cycle, both scatter point diagram and minimum spanning tree plot were drawn. The identified most stable genotypes with dynamic stability concept and based on the minimum spanning tree plots and centroid distances were G1 (3310.2 kg ha^-1) and G5 (3065.6 kg ha^-1), and therefore could be recommended for unfavorable or poor conditions. Also, genotypes G7 (3047.2 kg ha^-1) and G16 (3132.3 kg ha^-1) were located several times in the vertex positions of high cycles according to the principal coordinates analysis. The principal coordinates analysis provided useful and interesting ways of investigating GE interaction of barley genotypes. Finally, the results of principal coordinates analysis in general confirmed the breeding value of the genotypes, obtained on the basis of the yield stability evaluation.

Key words: grain yield, minimum spanning tree, yield stability

Corresponding author: Naser Sabaghnia,Department of Agronomy and Plant Breeding, Faculty of Agriculture,University of Maragheh, Maragheh, Iran. E-mail: sabaghnia@maragheh.ac.ir

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Published in „GENETIKA“ Vol. 45, No.3 (2013), pp. 703 -716

Serbia

UDC 575
DOI: :10.2298/GENSR1303703M

Original scientific paper

LIMITED AROMATIC PATHWAY GENES DIVERSITY AMONGST AROMATIC COMPOUND DEGRADING SOIL BACTERIAL ISOLATES

Gordana MINOVSKA, Tanja NARANČIĆ, Mina MANDIĆ, Lidija ŠENEROVIĆ, Branka VASILJEVIĆ and Jasmina NIKODINOVIĆ-RUNIĆ

Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia

Abstract

Minovska G., T. Narančić, M. Mandić, L. Šenerović, B.Vasiljević and J. Nikodinović-Runić (2013): Limited aromatic pathway genes diversity amongst aromatic compound degrading soil bacterial isolates.Genetika, Vol 45, No. 3,703 -716.

Identification and characterization of novel genes belonging to microbial aromatic biodegradation pathway is of great importance as they have been proven versatile biocatalysts. In this study, the selection of 19 environmental bacterial isolates capable to degrade a wide range of aromatic compounds has been screened for the presence of five genes from the lower and the upper aromatic biodegradation pathway using PCR methodology. In the case of 4-oxalocrotonate tautomerase and toluene dioxygenases, although present in the most of environmental isolates, very limited diversity of the genes has been encountered. Highly conserved sequences of these genes in environmental samples revealed high homology with gene sequences of the characterised corresponding genes from Pseudomonas putida species. The screen using degenerate primers based on known catechol- and naphthalene dioxygenases sequences resulted in a limited number of amplified fragments. Only two catechol 2,3-dioxygenase from two Bacillus isolates were amplified and showed no significant similarities with dioxygenases from characterized organisms, but 80-90% identities with partial catechol 2,3-dioxygenase sequences from uncultured organisms. Potentially three novel catechol 1,2-dioxygenases were identified from Bacillus sp. TN102, Gordonia sp. TN103 and Rhodococcus sp. TN112. Highly homologous tautomerase and toluene dioxygenases amongst environmental samples isolated from the contaminated environment suggested horizontal gene transfer while limited success in PCR detection of the other three genes indicates that these isolates may still be a source of novel genes.

Key words: Biodegradation, aromatic compounds, 4-oxalocrotonate tautomerase

Corresponding author: : Jasmina Nikodinović-Runić,Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, P.O.Box 23, 11010 Belgrade, Serbia

Telephone: +381 11 3976034,bFax: +381 11 3975808,E-mail: jasmina.nikodinovic@gmail.com

jasmina.nikodinovic@imgge.bg.ac.rs

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Published in „GENETIKA“ Vol. 45, No.3 (2013), pp. 717-726

Serbia

UDC 575
DOI: 10.2298/GENSR1303717O

Original scientific paper

MOLECULAR CHARACTERIZATION OF Prunus mahaleb L. ROOTSTOCK CANDITATES BY ISSR MARKERS

Ibrahim Kursat OZYURT¹, Yasar AKCA² and Sezai ERCISLI³

¹Middle Black Sea Transitional Zone Agricultural Research Station 60000 Tokat-Turkey

²Gaziosmanpasa University Agricultural Faculty Department of Horticulture 60240 Tokat-Turkey

³Ataturk University Agricultural Faculty Department of Horticulture 25240 Erzurum-Turkey

Abstract

Ozyurt K.I., Y.Akca and S. Ercisli (2013): Molecular characterization of Prunus mahaleb L. rootstock canditates by ISSR markers. Genetika, Vol 45, No. 3, 717-726.

Prunus mahaleb is widely used as rootstocks particularly on calcareous and dry soils for both sweet and sour cherry cultivars in Turkey. Genetic diversity and relationships among members of Prunus mahaleb including 29 pre-selected rootstock candidate accessions from Tokat region in Turkey were investigated by using 15 ISSR markers. The study revealed high genetic diversity among accessions, detecting 138 fragments, of which 103 (75%) were polymorphic. The number of polymorphic bands per primer was between 3-13, with average of 6.86. The primers 890 and 891 gave the highest polymorphism ratio (100%). The UPGMA dendrogram and the principal coordinate analysis revealed a clear differentiation among accessions. Reference rootstock, SL-64 clustered separately. The study demonstrates that ISSRs provide promising marker tools in revealing genetic diversity and relationships in Prunus mahaleb rootstock candidate accessions and can contribute to efficient identification, conservation, and utilization of germplasm for rootstock improvement through conventional as well as molecular breeding approaches.

Key words: Genetic diversity, ISSR, Mahaleb, Molecular characterization

Corresponding author: Sezai Ercisli, Ataturk University Agricultural Faculty Department of Horticulture 25240 Erzurum-Turkey, Phone: 090 4422312599, Fax: 090 4422360958, e-mail: sercisli@gmail.com

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Published in „GENETIKA“ Vol. 45, No. 3 (2013), pp. 727 - 736

Serbia

UDC 575

DOI: 10.2298/GENSR1303727B

Original scientific paper

THE EFFECT OF DIFFERENT ACUTE CONCENTRATIONS OF CADMIUM CHLORIDE ON THE FREQUENCY OF MICRONUCLEI IN AO RATS

Slavica POPOVIĆ BUBUJUK¹, Nenad Č. BOJAT²,Ninoslav DJELIĆ³, Slađana DRONJAK⁴, Ljiljana KOSTADINOVIĆ¹, Tamara COGHILL GALONJA², Marko ANDJELKOVIĆ⁵

¹Faculty of Biofarming, Megatrend University, Bačka Topola, Serbia

²Faculty of Economics and Engineering Management, Novi Sad, Serbia

³Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia

⁴Institute of Nuclear Sciences “Vinča”, Belgrade, Serbia

⁵Serbian Academy of Sciences and Arts, Belgrade, Serbia

Abstract

Popović Bubjuk S., N. Bojat, N. Djelić, S Dronjak, Lj. Kostadinović, T.Coghill Galonja and M. Andjelković (2013): The effect of different acute concentrations of cadmium chloride on the frequency of micronuclei in ao rats.Genetika, Vol 45, No. 3, 727-736.

Cadmium (Cd) is highly toxic heavy metal which may cause severe biological effects in vivo and in vitro. In this study, an evaluation of the acute Cd ability to trigger micronuclei (MNi) formation was carried out on 3-month-old male and female Albino Oxford (AO) rats using micronucleus (MN) test. Experimental animals were treated intraperitoneally with three different concentrations of cadmium chloride (CdCl₂): 0.5, 1, and 2 mg CdCl₂per kg of body weight. Control animals received equal volume of sterile phosphate buffered saline. The results showed that 2 mg CdCl₂per kg b.w. concentration caused a highly statistically significant (P < 0.001) increase in MNi formation in the bone marrow polychromatic erythrocytes (PCEs), exerting a clear-cut concentration-dependent effect. Lower concentrations of CdCl₂used (0.5 and 1 mg/kg b.w.) also caused MNi formation, but with lower statistical significance. Sex differences in MNi production in bone marrow PCEs after acute exposure to different experimental concentrations of CdCl₂ were not observed in our study. Our results indicate the ability of CdCl₂ to exerts genotoxic effects in bone marrow of AO rats, and complement previous data on the genotoxicity of this important environmental contaminant, burdening the body from different sources – major being industrial exposure, food and cigarette smoking.

Key words: Albino Oxford rats, cadmium, genotoxicity, micronucleus test

Corresponding author: Prof. Dr. Ninoslav Djelić, Department of Biology,Faculty of Veterinary Medicine,University of Belgrade, Oslobodjenja Blvd. 18,11000 Belgrade,Serbia,Phone: +381 11 265 88 94,Fax: +381 11 268 59 36,E-mail: ndjelic@vet.bg.ac.rs

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Published in „GENETIKA“ Vol. 45, No. 3(2013), pp. 737 - 736

Serbia

UDC 575:630
DOI: 10.2298/GENSR1303737A

Original scientific paper

GENETIC ANALYSIS OF PARTIAL RESISTANCE TO BASAL STEM ROT (Sclerotinia sclerotiorum) IN SUNFLOWER

Masoumeh AMOUZADEH¹, Reza DARVISHZADEH^*,1, Parham HADDADI², Babak ABDOLLAHIMANDOULAKANI¹, Younes REZAEE DANESH³

¹Department of Plant Breeding and Biotechnology, Urmia University, Urmia, Iran.

²INRA, Institute Jean-Pierre Bourgin (IJPB), Versailles Cedex France.

³Departeman of Plant Protection, Urmia University, Urmia, Iran.

Abstract

Amouzadeh M., R. Darvishzadeh, P. Haddadi, B. Aabdollahi Mandoulakani, and Y. Rezaee Danesh (2013): Genetic analysis of partial resistance to basal stem rot (Sclerotinia sclerotiorum) in sunflower Genetika, Vol 45, No. 3, 737-748.

Basal stem rot, caused by Sclerotinia sclerotiorum (Lib.) de Bary, is one of the major diseases of sunflower (Helianthus annuus L.) in the world. Quantitative trait loci (QTLs) implicated in partial resistance to basal stem rot disease were identified using 99 recombinant inbred lines (RILs) from the cross between sunflower parental lines PAC2 and RHA266. The study was undertaken in a completely randomized design with three replications under controlled conditions. The RILs and their parental lines were inoculated with a moderately aggressive isolate of S. sclerotiorum (SSKH41). Resistance to disease was evaluated by measuring the percentage of necrosis area three days after inoculation. QTLs were mapped using an updated high-density SSR and SNP linkage map. ANOVA showed significant differences among sunflower lines for resistance to basal stem rot (P≤0.05). The frequency distribution of lines for susceptibility to disease showed a continuous pattern. Composite interval mapping analysis revealed 5 QTLs for percentage of necrotic area, localized on linkage groups 1, 3, 8, 10 and 17. The sign of additive effect was positive in 5 QTLs, suggesting that the additive allele for partial resistance to basal stem rot came from the paternal line (RHA266). The phenotypic variance explained by QTLs (R²) ranged from 0.5 to 3.16%. Identified genes (HUCL02246_1, GST and POD), and SSR markers (ORS338, and SSL3) encompassing the QTLs for partial resistance to basal stem rot could be good candidates for marker assisted selection.

Key words: Basal stem rot, Helianthus annuus L., heritability, partial resistance, QTL mapping.

Corresponding author: Dr. R. Darvishzadeh, Department of Plant Breeding and Biotechnology, Urmia University, Urmia, Iran. E-mail: r.darvishzadeh@urmia.ac.ir. Phone: + 98 441 2972785. Fax: + 98 441 2779558

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Published in „GENETIKA“ Vol. 45, No.3(2013), pp. 749 -760

Serbia

UDC 575:633.11
DOI: 10.2298/GENSR1303749K

Original scientific paper

MATING POPULATIONS OF Gibberella fujikuroi (Sawada) S. ITO SPECIES COMPLEX ISOLATING FROM MAIZE, SORGHUM AND WHEAT IN SERBIA

Tamara KOVAČEVIĆ¹, Jelena LEVIĆ¹, Slavica STANKOVIĆ¹,

Jelena VUKOJEVIĆ²

¹Maize Research Institute, Zemun Polje, Belgrade-Zemun, Republic of Serbia

²University of Belgrade, Faculty of Biology, Belgrade, Republic of Serbia

Abstract

Kovačević T., J. Lević, S. Stanković, and J. Vukojević (2013): Mating populations of Gibberella fujikuroi (sawada) s. ito species complex isolating from maize, sorghum and wheat in Serbia. Genetika, Vol 45, No. 3, 749-760.

The status of fertility and distribution of mating populations in the G. fujikuroi species complex, isolating from maize, sorghum and wheat cultivated under various agroecological conditions of Serbia, have been studied. A total of 79 field isolates of Fusarium spp. in the section Liseola, which had been reciprocally crossed to standard testers (MAT-1 and MAT-2) from each of the four mating populations of the G. fujikuroi species complex, were selected for these studies. Twenty of 79 isolates belonged to the mating population A (G. moniliformis), 22 to the population D (G. intermedia), 17 to the population E (G. subglutinans) and 20 to the population F (G. thapsina). A mating type MAT-1 was dominant in the populations A (14 MATA-1 : 6 MATA-2), D (13 MATA-1 : 9 MATA-2) and E (10 MATA-1 : 7 MATA-2), while MAT-2 prevailed in the population F (6 MATA-1 : 14 MATA-2). The obtained results indicate that the possibility of sexual reproduction of Fusarium spp., belonging to the A, D, E and F mating populations, is not so frequent phenomenon in Serbia as in other regions world-wide. Consequently, these species will be asexually reproduced under field conditions, particularly species belonging to the F population. These are the first results on the characterisation of three (A, E and F) out of four populations of the G. fujikuroi species complex present in Serbia.

Key words: Gibberella fujikuroi, A, E, D and F populations, mating type, cereals

Corresponding author: Jelena Lević, Maize Research Institute, Zemun Polje, Slobodana Bajića 1, 11 185 Belgrade, Republic of Serbia,Fax number: +381 11 37 56 707,E-mail address: jlevic@mrizp.rs

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Published in „GENETIKA“ Vol. 45, No. 3(2013), pp. 761-768

Serbia

UDC 575
DOI: 10.2298/GENSR1303761K

Original scientific paper

DISTRIBUTION OF SEX CHROMOSOMES (XY) IN LYMPHOCYTE METAPHASE SPREADS OF DAIRY BULLS

Rosaiah KOTIKALAPUDI, Rajesh Kumar PATEL, Hemanth MEDIDI

and Nagaraju Naik SUGALI

Sandor Proteomics, Banjara Hills, Hyderabad-500 034, India

Abstract

Kotikalapudi R., R. Kumar Patel, H. Medidi and N. Naik Sugali (2013): Distribution of sex chromosomes (XY) in lymphocyte metaphase spreads of dairy bulls. Genetika, Vol 45, No. 3, 761-768.

Position of autosome and sex chromosomes in metaphase spreads is grate concerned of Cytogeneticians worldwide to understand cell biology. A few isolated studies have been conducted for the distribution of chromosomes in metaphase spread. Our studies reveal that most sex chromosomes (XY) remain on periphery and semi-periphery, 84.16% for X and 86.97% for Y respectively, in round metaphase spreads. The application of sex chromosome position in metaphase spreads is to easily find out sex chromosomes under microscope even without banding patterns. An another application is to identify or confirm sex chromosomes in unknown species on which cytogenetic studies have not been performed.

Key words: dairy bulls, methaphase, sex chromosomes

Corresponding author: Dr R K Patel Sandor Proteomics, Banjara Hills, Hyderabad-500 034, India, rkpatel46@yahoo.com

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Published in „GENETIKA“ Vol. 45, No.3(2013), pp. 769 - 776

Serbia

UDC 575
DOI: 10.2298/GENSR1303769J

Original scientific paper

THE POSSIBILITIES OF APPLYING MARKER ASSISTED SELECTION IN BREEDING BORON TOLERANT WHEAT

Milka BRDAR-JOKANOVIĆ¹, Dragana TRKULJA¹, Emilija NIKOLIĆ-ĐORIĆ², Ankica KONDIĆ-ŠPIKA¹, Borislav KOBILJSKI¹

¹Institute of Field and Vegetable Crops, Novi Sad, Serbia

²University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia

Abstract

Jokanović-Brdar M., D. Trkulja, E .Nikolić-Đorić, A. Kondić-Šipka, and B. Kobiljski (2013): The possibilities of applying marker assisted selection in breeding boron tolerant wheat. Genetika vol 45, No3, 769-776.

The aim of this study was to investigate the possibilities of applying molecular markers-microsatellites in breeding boron tolerant wheat. The study comprised the investigation of allelic variability of sixty bread wheat accessions in two microsatellite loci (Xgwm46-7B and Xgwm577-7B) for which was assumed that are placed near the 7B chromosome locus involved in the expression of boron tolerance in wheat. Phenotypic variability concerning boron tolerance was assessed via root length reduction of wheat seedlings grown in the presence of high external boron, applied as boric acid solution (concentrations 50, 100 and 150 mg/l, boron treatments B50, B100 and B150). The indication of marker-trait associations was determined by comparing the allelic variability in the two microsatellite loci with the phenotypic variability in boron tolerance. Nonparametric Kruskal-Wallis test was used for the comparisons. The indication of marker-trait association was found for both Xgwm46-7B and Xgwm577-7B; on B150 and B50 treatments, respectively. Allelic forms identified in Xgwm577-7B locus may be related to tolerance, medium tolerance and sensitivity to high boron. This was not the case for Xgwm46-7B, where the identified alleles were related only to boron tolerance and sensitivity. Therefore, Xgwm577-7B may be preferred over Xgwm46-7B when studying boron tolerance in wheat. However, a considerable portion of boron tolerant accessions carried different alleles in the investigated loci, implying boron tolerance as a quantitative trait with more than one chromosomal region involved in its expression. Therefore, the allelic variability of more than the analyzed two loci should be investigated.

Key words: wheat, boron tolerance, marker-assisted selection, microsatellites

Corresponding author: Milka Brdar-Jokanović, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia, Phone: 381 21 4898 208, Fax: 381 21 4898 222, E-mail: milka.brdar@nsseme.com, milka.brdarjokanovic@gmail.com

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Published in „GENETIKA“ Vol. 45, No. 3(2013), pp. 777- 791

Serbia

UDC 575

DOI: 10.2298/GENSR1303777K

Original scientific paper

EFFECTS OF SALT STRESS ON WILD TYPE AND VTE₄MUTANT Arabidopsis thaliana: MODEL PLANT TO ENGINEER TOLERANCE TOWARDS SALINITY

Amir Ali KHALATBARI¹, Hawa Z.E. JAAFAR¹,*, Maziah MAHMOOD ², Radziah OTHMAN³, Amir Mahdi KHALATBARI¹

¹⁾ Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia

2) Department of Biochemistry, Faculty of Biotechnology and Biomolecular sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia

³⁾ Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia

Abstract

Khalatbari A.A., H. Z.E. Jaffar, M. Mahmood, R. Othman and A.M.Khalatbari (2013): Effects of salt stress on wild type and vte₄mutant Arabidopsis thaliana: model plant to engineer tolerance towards salinity. Genetika, Vol 45, No. 3, 777-791.

One of the major environmental constraints impairing plant distribution and yield is believed to be salt stress. Additionally, engineered abiotic stress resistance or/and tolerance is considered as an indispensable target in order to enhance plant productivity. In this study, the effects of salinity on physiological and morphological of wild type (Columbia-0) and vte₄ mutant Arabidopsis thaliana were investigated under different NaCl concentrations. These salt treatments, including control condition, 50mM and 100mM NaCl were imposed on the plants. Each salt treatment was replicated three times in a complete randomized design with factorial arrangement. Wild type and mutant A.thaliana plants were subjected to the abiotic stress (salinity) for up to 11 days to evaluate the parameters of growth, development and water relations. As a result, the performance of wild type plants was stronger than vte₄ mutant under different salt treatments. Under control condition, rosette dry weight, maximum quantum efficiency (PSII) and specific leaf area obtained the highest values of 13.85 mg, 0.81 Fv/Fm and 49.84 mm²/mg, respectively. As salt treatments continued both wild type and vte₄ mutant plants were affected negatively. When plant types was considered, wild type A.thaliana recorded higher value of 0.82 gW/gFW for relative water content (RWC) under 50mM NaCl whereas mutant plants gained the value of 0.78 gW/gFW under the same condition. However, root mass fraction indicated an increase for both wild type and vte₄mutant plants after 11 days of salt stress onset. The reduction of water potential was observed for wild type and mutant A.thaliana where it scored -1.3 MPa and -1.4, respectively. As a conclusion, these findings implied that under different salt treatments morphological and physiological responses of wild type and vte₄ mutant were affected in which wild type plants showed more tolerance. Lack of γ-tocopherol methyltransferase (γ -TMT) gene in vte₄ seemed to impair defence mechanism of this mutant against salinity.

Key words: Arabidopsis thaliana; mutant, salt stress, wild type

Corresponding author: Hawa Z.E. Jaafar, Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia, 43400,Phone number: 0060389471807- 00989369439618, drhawazej.postgrads@gmail.com.

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Published in „GENETIKA“ Vol. 45, No. 3(2013), pp. 793- 798

Serbia

UDC 575
DOI: 10.2298/GENSR1303793B

Original scientific paper

CHROMOSOME STATUS OF MARSH MARIGOLD, Caltha palustris L. (Ranunculaceae) FROM SERBIA

Jelena BLAGOJEVIĆ*, Vladimir JOVANOVIĆ, Tanja ADNAĐEVIĆ, Ivana BUDINSKI and Mladen VUJOŠEVIĆ

University of Belgrade, Institute for Biological Research ”Siniša Stanković“, Department of Genetic Research, Belgrade, Serbia

Abstract

Blagojević J., V. Jovanović, T. Andađević, I. Budinski, and M. Vujošević (2013): Chromosome status of marsh marigold, Caltha palustris L. (Ranunculaceae) from Serbia. Genetika, Vol 45, No. 3, 793-798

Marsh marigold, Caltha palustris is distributed in the moist, temperate and cold regions of the Northern Hemisphere. This species exhibits considerable amount of intraspecific chromosomal diversity involving hybridization, polyploidy, aneuploidy and B chromosomes. Karyotype analyses of three mountain populations from Serbia were done for the first time. All samples were tetraploid (based number x=8) with 2n=32. In population from mountain Tara presence of one B chromosomes was detected. Tetraploid karyotype consists of 17 median-centromeric (m), 8 submedian-centromeric (sm), 7 subterminal-centromeric (st) chromosomes and one terminal-centromeric (t) B chromosome (2n= 17m+8sm+7st+1B). Studied populations in Serbia belong to the most common cytotype for this species in Europe.

Key words: Caltha palustris, polyploidy, B chromosomes

Corresponding author: Jelena Blagojević, Department of Genetic Research, Institute for Biological Research ”Siniša Stanković“, University of Belgrade, Blvd despota Stefana 142, 11060 Belgrade, Serbia. phone +381 11 2078 331; fax. +381 11 2764 422; e-mail: jelena.blagojevic@ibiss.bg.ac.rs

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Published in „GENETIKA“ Vol. 45, No. 3(2013), pp. 799-810

Serbia

UDC 575
DOI: 10.2298/GENSR1303799Y

Original scientific paper

BIOCOMPUTATIONAL GENOME-WIDE ANALYSIS OF MICRO RNA GENETIC

VARIABILITY IN SOME VERTEBRATES

Abdulmojeed YAKUBU¹, Ibrahim Suleiman MUSA-AZARA², Blessing N. Saleh YAKUBU², Sylvester I. DAIKWO³, Samuel Ter VINCENT⁴, Ojo Michael MOMOH⁴ and Ndubuisi I. DIM⁴

¹Department of Animal Science, Faculty of Agriculture, Nasarawa State University, Keffi, Lafia, Nasarawa State, Nigeria.

²Department of Animal Science, College of Agriculture, Lafia, Nasarawa State, Nigeria.

³Department of Animal Science, Federal University, Wukari, Taraba State, Nigeria.

⁴Department of Animal Breeding and Physiology, University of Agriculture, Benue State, Nigeria.

Abstract

Yakubu A., I. S. Musa-Azra, B.N.S. Yakubu, S.I.Daikwo, S.T.Vincet. O.M.Momoh, and N.I.Dim (2013): Biocomputational genome-wide analysis of microrna genetic variability in some vertebrates. Genetika, Vol 45, No. 3, 799-810.

MicroRNAs (miRNAs) are small endogenously expressed single-stranded RNAs that regulate gene expression post transcriptionally and shape diverse cellular pathways. miRNAs regulate a wide range of biological processes through the recognition of complementary sequences between miRNAs and their target genes. The present investigation aimed at determining in-silico the genetic variability of miRNA genes in some livestock and non-livestock species. Effects of single nucleotide polymorphisms (SNPs) in genes’ 3'UTR on target gain/loss of human miRNAs were also explored. A total of twenty four mature miRNA sequences and genomic coordinates in three livestock [chicken (5), pig (1) and cattle (9)] and two non-livestock (human (6) and mouse (3)] species were retrieved from the miRBase 15 release. Computational scanning of polymorphisms in the miRNAs revealed 33 and 20 polymorphic sites in livestock and non-livestock species, respectively. Of this, 7 (chicken), 11 (cattle) and 2 (mouse) were located within the seed region. The de novo computational prediction revealed that SNPs rs1042725 (C/U) and rs1044129 (A/G) in genes’ 3'UTR of human miRNAs positively influenced the target site thereby resulting in target gain. However, the effects of SNPs rs56109847 (A/G), rs28927680, rs12720208 (G/A) and rs5186 (A/C) were negative. The evolutionary tree showed that the relationship between miRNA consensus sequences of livestock (pig, chicken and cattle) was closer compared to non-livestock species (mouse and human), which could be implicated in morphological complexity among vertebrates. Although the function of miRNA is only beginning to be understood, future in-silico research evaluating the functional effect of miRNA in gene translation and subsequent biological pathways especially in livestock is of paramount importance; and this should be complemented with hypothesis-driven experimental studies to evaluate the phenotypic effect of identified miRNA genetic polymorphisms in animals.

Key words: miRNAs, polymorphisms, in-silico, functional effect, animals.

Corresponding author: Abdulmojeed Yakubu,Department of Animal Science, Faculty of Agriculture, Nasarawa State University, Keffi, Shabu-Lafia Campus, P.M.B. 135, Lafia, Nasarawa State, Nigeria, Mobile No: +2348065644748, Email: abdulmojyak@gmail.com

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Published in „GENETIKA“ Vol. 45, No. 3(2013), pp. 811-824

Serbia

UDC 575:633.15
DOI: 10.2298/GENSR1303811R

Original scientific paper

GENETIC DIVERSITY IN MAIZE DENT LANDRACES ASSESSED BY MORPHOLOGICAL AND MOLECULAR MARKERS

Danijela RISTIĆ, Vojka BABIĆ, Violeta ANĐELKOVIĆ, Jelena VANČETOVIĆ, Snežana MLADENOVIĆ DRINIĆ, Dragana IGNJATOVIĆ-MICIĆ

Maize Research Institute „Zemun Polje“, Belgrade, Serbia

Abstract

Ristić D., V Babić, V. Anđelković, J. Vančetović, S. Mladenović Drinić and D. Ignjatović Micić (2013): Genetic diversity in maize dent landraces assessed by morphological and molecular markers Genetika, Vol 45, No. 3, 811-824.

Maize Research Institute “Zemun Polje” genebank maintains a collection of landraces grouped into 18 agro-ecological collected from ex-Yugoslavia territories. The application and comparison of different marker systems are important for the characterization and use of maize landraces in breeding program, as potential sources of desirable traits. In this study, 15 morphological traits, 7 RAPD primers and 10 SSR primer pairs were applied to i) to determine genetic distance between 21 maize dent landraces and ii) compare results obtained on morphological and molecular markers. Phenotypic analysis showed high level of heterogeneity between landraces. Higher level of genetic diversity was obtained with SSR than with RAPD. Genetic distance mean value for RAPD data was 0.35 i.e. for SSR 0.48. Based on the morphological traits and molecular markers, unweighted pairgroup method (UPGMA) analysis was applied for cluster analysis, using statistical NTSYSpc program package. Cluster analysis of morphological and molecular markers distances did not show the same population grouping. Better agreement with agro-ecological data was obtained with RAPD markers. Correlations between dissimilarity matrices for different types of markers were low. Data obtained in this work could be useful for further study of a larger number of landraces, and conservation of genetic resources and their genetic diversity.

Key words: maize dent landraces, RAPD, SSR

Corresponding author: Danijela Ristić Maize Research Institute „Zemun Polje“, Slobodana Bajića 1, 11185 Zemun Polje, tel 0113756704, fax 011 3756707,dristic@mrizp.rs

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Published in „GENETIKA“ Vol. 45, No. 3(2013), pp. 825-835.

Serbia

UDC 575
DOI: 10.2298/GENSR1303825L

Original scientific paper

USING OF AFLP TO EVALUATE GAMMA-IRRADIATED AMARANTH MUTANTS.

Mária LABAJOVÁ¹, Jana ŽIAROVSKÁ¹, Katarína RAŽNÁ¹, Jaroslava OVESNÁ²,

Andrea HRICOVÁ³

¹ FAPZ, SPU v Nitre, Slovak Republic

² VÚRV, Praha, Czech Republic

³ ÚGBR, SAV, Nitra, Slovak Republic

Abstract

Labajova M., J. Žiarovska, K. Ražna, J. Ovesna and A. Hricova (2013): Using of AFLP to evaluate gamma-irradiated amaranth mutants.. Genetika, Vol 45, No. 3, 825-835.

To determine which of several gamma-irradiated mutants of amaranth Ficha cultivar and K-433 hybrid are most genetically similar to their non-irradiated control genotypes, we performed amplified fragment length polymorphism (AFLP) based analysis. A total of 40 selective primer combinations were used in reported analyses. First analyses of gamma-irradiated amaranth mutant lines were done used the AFLP. In the study, primers with the differentiation ability for all analysed mutant lines are reported. The very specific changes in the mutant lines´ non-coding regions based on AFLP length polymorphism were analysed. Mutant lines of the Ficha cultivar (C15, C26, C27, C82, C236) shared a genetic dissimilarity of 0,11 and their ISSR profiles are more similar to the Ficha than those of K-433 hybrid mutant lines. The K-433 mutant lines (D54, D279, D282) shared genetic dissimilarity of 0,534 but are more distinct to their control plant as a whole, as those of the Ficha mutant lines. Different AFLP fingerprints patters of the mutant lines when compared to the Ficha cultivar and K-433 hybrid AFLP profiles may be a consequence of the complex response of the intergenic space of mutant lines to the gamma-radiance. Although a genetic polymorphism was detected within accessions, the AFLP markers successfully identified all the accessions. The AFLP results are discussed by a combination of biochemical characteristics of mutant lines and their control genotypes.

Key words: AFLP, gamma-radiance, amaranth mutant lines, Ficha, K-433

Corresponding author: Jana Žiarovská,: FAPZ, SPU v Nitre, Slovak Republic, jana.ziarovska@uniag.sk

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Published in „GENETIKA“ Vol. 45, No. 3(2013), pp. 837-853.

Serbia

UDC 575
DOI: 10.2298/GENSR1303837R

Original scientific paper

MOLECULAR CLONING AND IN SILICO ANALYSIS OF THREE SOMATIC EMBRYOGENESIS RECEPTOR KINASE mRNA FROM DATE PALM

Imen REKIK, Amine ELLEUCH, Walid KRIAA, Noureddine DRIRA

Laboratory of plant biotechnology, Faculty of sciences of Sfax, University of Sfax. Tunisia

Abstract

Rekik I., A.. Elleuch, W. Kriaa and N. Drira (2013): Molecular cloning and in silico analysis of three somatic embryogenesis receptor kinase mRNA from date palm. Genetika, Vol 45, No. 3, 837-853.

We report here the isolation and characterizations of three somatic embryogenesis receptor kinase (PhSERK) genes from palm date by a rapid amplification of cDNA ends (RACE) approach. PhSERKs belong to a small family of receptor kinase genes, share a conserved structure and extensive sequence homology with previously reported plant SERK genes. Sequence analysis of these genes revealed the sequence size of 11051 pb (PhSERK1), 7981 pb (PhSERK2) and 10510 pb (PhSERK3). The open reading frames of PhSERK1, PhSERK2 and PhSERK3 are 1914 pb, 1797 pb and 1719 pb respectively. PhSERKs belongs to the LRR-type cell surface RLKs, which possess a number of characteristic domains. These include an extracellular domain (EX) containing a variable number of LRR units, signal pepetide (SP) immediately followed by a single transmembrane domain (TM) and an intracellular kinase domain. The phylogenetic tree shows that the protein PhSERK1, PhSERK2 and PhSERK3 clustered within monocots SERKs proteins groups. We also predicted the secondary and tertiary with ligand binding sites structure of the protein PhSERKs.

Key words: Phoenix dactylifera; Phylogeny; Protein; Somatic embryogenesis receptor kinase; Structure

Corresponding author: Imen Rekik, Faculty of sciences of Sfax, Sfax, Tunisia. Tel: 0021674676616, Fax: 0021674274437, email: imenbmc@yahoo.fr

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Published in „GENETIKA“ Vol. 45, No. 3(2013), pp. 855-864

Serbia

UDC 575:630
DOI: 10.2298/GENSR1303853M

Original scientific paper

IDENTIFICATION OF QTLS ASSOCIATED WITH LOW CHLORIDE ACCUMULATION IN ORIENTAL TOBACCO

Hamid HATAMI MALEKI¹, Ghasem KARIMZADEH^1*, Reza DARVISHZADEH², Mohammad Reza NAGHAVI³ and Ahmad SARRAFI⁴

¹Plant Breeding and Biotechnology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

²Department of Agronomy and Plant Breeding, Urmia University, Urmia, Iran

³Agronomy and Plant Breeding Department, Agricultural College, University of Tehran, Karaj, Iran

⁴Laboratoire Ecologie Fonctionnelle, EcoLab, Dynabio, INP-ENSAT, Castanet-Tolosan, France

Rehabilitation Sciences, Tehran, Iran.

Abstract

Maleki Hatami H., G. Karimzadeh, R. Darvishzadeh, M.Rezanaghavi and A.Sarrafi (2013): Identification of QTLs associated with low chloride accumulation in oriental tobacco. Genetika, Vol 45, No. 3, 855-864.

The construction of linkage maps and identification of genomic regions controlling traits have great significance for plant breeders. Among different chemical traits in oriental tobacco, chloride content in leaves when it is less than 1.5% improves burning quality. In this study, genetic analysis of quantitative trait loci affecting the chloride accumulation of oriental tobacco leaf was performed using an F₂ population of a cross between two oriental tobacco genotypes, ‘Basma Seres 31’ and ‘SPT 406’, comprising 100 individuals. A normal distribution was observed for the chloride accumulation in the F₂ population. Linkage map with 23 informative microsatellite and 29 inter simple sequence repeat markers was constructed, which covered 570.8 cM of the tobacco genome. Single marker analysis, interval mapping and composite interval mapping were used to detect the putative QTLs controlling chloride accumulation. There was not any significant relation between ISSR markers and chloride accumulation. SSR marker PT30346 was found to be significantly associated with chloride accumulation through single marker analysis. Two QTLs including Chl_IM and Chl_CIMwith R² values of 0.4 and 0.07 were identified using IM and CIM, respectively.

Key words: Chloride accumulation, Linkage map, Oriental tobacco, QTL

Corresponding author: Ghasem Karimzadeh, Tarbiat Modares University, Faculty of Agriculture, Plant Breeding and Biotechnology Department, Tel.: +98 21 48292103, fax: +98 21 48292200. E-mail address: karimzadeh_g@modares.ac.ir

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Published in „GENETIKA“ Vol. 45, No. 3(2013), pp. 865-872

Serbia

UDC 575
DOI: 10.2298/GENSR1303865M

Original scientific paper

DISTRIBUTION OF Apolipoprotein E gene polymorphism in

students and in high-educated elderly from Serbia

Nela MAKSIMOVIĆ, Ivana NOVAKOVIĆ, Vesna RALIĆ and Elka STEFANOVA

Faculty of Medicine, University of Belgrade, Belgrade, Serbia

Abstract

Maksimović N, I. Novaković, V. Ralić, and E.Stefanova (2013): Distribution of apolipoprotein e gene polymorphism in students and in high-educated elderly from Serbia. Genetika, Vol 45, No. 3, 865-872.

Apolipoprotein E (ApoE) play important role in lipid metabolism and in processes of remodeling and reparation in central nervous system. Three common ApoE isoforms, ApoE2, ApoE3 and ApoE4, show strong genetic determination by ε2, ε3, and ε4 allele. In human genome gene encoding Apolipoprotein E (APOE) is located on cromosome 19, and ε2/ε3/ε4 haplotype system is defined by 2 non-synonymous single nucleotide polymorphisms (SNPs) in the APOE exon 4. The frequency of the three APOE alleles and corresponding genotypes varies across human populations, with possible clinical implications. At least, variable distribution of ε4 allele may contribute to the regional risk of cardiovascular and Alzheimer’s diseases. Allele-frequency comparisons between younger and older populations suggest an effect of APOE on mortality, but these data are not consistently confirmed. In the present study we have analyzed the distribution of APOE gene polymorphism in a group of University students and retained University professors living in Serbia. After DNA extraction from peripheral blood samples, the APOE genotype was determined by polymerase chain reaction (PCR) followed with HhaI restriction digestion. We found no statistically significant difference in alleles and genotypes distribution between younger and elder group of participants. Also, there was no significant difference compared to APOE data previously obtained in YUSAD cohort of healthy school children (15 y of age) from different regions of Serbia. In both of our groups, as well as in YUSAD cohort, frequency of APOE ε4 allele was <10%. The observed frequencies are lower than in neighboring countries, but similar with Spanish data and some Asian populations. Our results do not support important role of APOE ε4 in the morbidity and mortality in Serbian population, but gene-environmental-social interactions should be considered.

Key words: apolipoprotein E, gene polymorphism, population study, Serbia, ε4 allele

Corresponding author: Ivana Novaković, Faculty of Medicine, University of Belgrade,dr Subotića 8, 11000 Belgrade, Serbia, phone: +381 11 3607040. fax: +381 11 3607042, email: novivana@eunet.rs

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Published in „GENETIKA“ Vol. 45, No. 3(2013), pp. 873-880

Serbia

UDC 575.630
DOI: 10.2298/GENSR1303873A

Original scientific paper

ANALYSIS OF POMOLOGICAL TRAITS IN NEW PROMISING SWEET CHERRY GENOTYPES

Milica FOTIRIĆ AKŠIĆ^1* and Tihomir NIKOLIĆ²

¹ University of Belgrade, Faculty of Agriculture, Belgrade - Zemun, Serbia

²Orchards and wine company `Grocka`, Belgrade, Serbia

Abstract

Akšić Fotirić M., and T. Nikolić (2013): Analysis of pomological traits in new promising sweet cherry genotypes Genetika, Vol 45, No. 3, 873--880.

Sweet cherry breeding programs are interested in developing new cultivars that are very early or very late in ripening, high yielding, with excellent fruit size and quality. The aim of this study was to evaluate new, promising, sweet cherry genotypes (G-1 and G-2) for ripening time, fruit morphology and fruit chemical content, and compare it with the standard sweet cherry cultivars (Bigarreau Jaboulay, B. H. Burlat, Seneca, Hedelfiger Riesenkirsche, Germersdorfer Grosse Kirsche, Emperor Francis and Bing). Experiment was done during four consecutive years (2006-2009) in Grocka, near Belgrade. The earliest fruit ripening was recorded in G-2 (22^ndApril) while the latest in `Bing` (2^ndJune). Fruit weight varied from 5.83 g (G-2) up to 8.93 g (G-1). Soluble solid content and total sugar content were the lowest in genotype G-2 (10.48%; 8.45%, respectively) but the highest in Emperor Francis (18.28%; 15.98%, respectively). No matter the fact that some standard cultivars showed better results for some traits, genotypes G-1 and G-2 are considered very promising. So, genotype G-1 could be intended for table consumption since it ripens 2-3 days after cv. B. H. Burlat, but have much higher fruit weight. Also, genotype G-2, the earliest in this trial, showed satisfactory fruit weight for this ripening time, which makes it very interesting for fresh market production.

Key words: ANOVA, chemical traits, coefficient of variation, fruit, ripening time

Corresponding author: Milica Fotirić Akšić, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade-Zemun, Serbia, phone: ++ 381 64 2612710, fax: ++ 381 11 2199 805, e-mail: fotiric@agrif.bg.ac.rs

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Published in „GENETIKA“ Vol. 45, No. 3(2013), pp. 881-893

Serbia

UDC 575
DOI: 10.2298/GENSR1303881V

Original scientific paper

ASSOCIATION STUDY OF FIVE MUTATION IN FGFR1 AND FGFR2 GENES IN INDIAN CHILDREN WITH CRANIOSYNOSTOSIS

Rajeev Kumar PANDEY, Abid ALI, Minu BAJPAI, Sukanya GAYAN, Amit SINGH

^.Department of Pediatric surgery, All India Institute of Medical Sciences, New Delhi-110029

Abstract

Pandey Kumar R., A. Ali, M. Bajpai, S. Gayan and A. Singh (2013): Association study of five mutation in FGFR1 and FGFR2 genes in Indian children with craniosynostosis. Genetika, Vol 45, No. 3, 881-893.

Background: Craniosynostosis is one the major genetic disorder in children and it occurs in 1 per 2,200 live births. It may be define as abnormal premature fusion of the cranial sutures bones in children. Several causes have been reported that may have a possible role in the development of the disorder. Fibrinogen growth Factor 1(FGFR1) & fibroblast growth factor receptor 2 (FGFR2) show a vital role in developing the craniosynostosis in western population’s children but from India no report is available. The aim of this study was to investigate the association between mutation of FGFR1 and FGFR2 (IIIa and IIIb) genes with syndromic as well as non-syndromic craniosynostosis in Indian population.

Methods: Retrospective analysis of our records from January 2008 to December 2012 was done. A total of Sixty three children (along with their parents) with craniosynostosis and Fifty one children with No- craniosynostosis (healthy school going children) attending the Monday out Patient Door (OPD) facility of the department of Paediatric Surgery, All India Institute of Medical Sciences (AIIMSs), Delhi, India were considered for the study. A restriction fragment length polymorphism (RFLP) polymerase chain reaction (PCR) was carried out for genotyping Fibrinogen growth Factor 1 (FGFR1) & fibroblast growth factor receptor 2 (FGFR2) mutations in all the participants.

Results: There were 33 (80.4%) nonsyndromic cases of craniosynostosis while 8 (19.5%) were syndromic. Out of these 8 syndromic cases, 4 were Apert syndrome, 3 were Crouzon syndrome and 1 Pfeiffer syndrome. Phenotypically the most common nonsyndromic craniosynostosis was scaphocephaly (19, 57.7%) followed by plagiocephaly in (14, 42.3%). FGFR1 mutation (Pro252Arg) was seen in 1 (2.4%) case of nonsyndromic craniosynostosis while no association was noted either with FGFR1 or with FGFR2 (IIIa & IIIc) mutation in syndromic cases. None of the control group showed any mutation.

Conclusion: Our study provides the strongest evidence that association of mutation of FGFR1, FGFR2 (IIIa & IIIb) with syndromic as well as nonsyndromic craniosynostosis does not exist in Indian population as seen in western population.

Key words: Craniosynostosis, Fibrinogen growth Factor, Suture, Restriction fragment length polymorphism (RFLP)

Corresponding author: Dr. M. Bajpai, Department of Paediatric Surgery,All India Institute of Medical Sciences,Ansari Nagar, New Delhi-110029

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Published in „GENETIKA“ Vol. 45, No. 3(2013), pp. 895-906

UDC 575:630
DOI: 10.2298/GENSR1303895B

Original scientific paper

GENETIC DIFFERENTIATION IN SEED STANDS OF EUROPEAN BEECH

(Fagus sylvatica L.) IN PART OF BOSNIA AND HERZEGOVINA

Dalibor BALLIAN¹, Vasilije ISAJEV², Vanja DANIČIĆ², Branislav CVJETKOVIĆ²,

Faruk BOGUNIĆ¹, Milan MATARUGA²

¹ Faculty of Forestry, University of Sarajevo, Sarajevo, Bosnia and Herzegovina.

²Faculty of Forestry, University of Banja Luka, Bosnia and Herzegovina

Abstract

Ballian D, V. Isajev, V. Daničić, B. Cvetković, F. Bogunić and M. Mataruga (2013): Genetic differentiation in seed stands of European beech (Fagus sylvatica L.) in part of Bosnia and Herzegovina. Genetika, Vol 45, No. 3, 895-906

Beech (Fagus sylvatica L.) is one of the most important forest trees in Bosnia and Herzegovina in both economic and environmental terms. The total area of forest in which beech is present is approx. 1,652,400 ha. There is a proportionate need to plant new forests and produce genetically high quality seed and saplings.Biochemical analysis of the genetic structure of eight populations of beech using ten enzyme systems from 16 isoenzyme gene loci revealed significant differences between the populations analyzed. Variance levels were high in some gene loci, while in some populations monomorphism was recorded only for individual gene loci. The average number of alleles per locus ranged from 2.1875 to 2.5625, and the average number of genotypes per locus varied from 2.6875 to 3.2500. The multilocus genetic diversity at the population level ranged from 63.276 to 162.001, and the genofund diversity varied from 1.2708 to 1.3416.The average differentiation value obtained for all populations was fairly low (Dj=5.81), indicating a percentage of overall variance of about 94.194%.

Key words: European beech (Fagus sylvatica L. s.l.), seed stand, variability, isoenzyme, differentiation, Bosnia and Herzegovina

Corresponding author: Dalibor Ballian, Faculty of Forestry, Zagrebačka 20, University of Sarajevo, Sarajevo, Bosnia and Herzegovina. balliandalibor9@gmail.com

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Published in „GENETIKA“ Vol. 45, No. 3(2013), pp. 907-920

Serbia

UDC 575:633
DOI: 10.2298/GENSR1303907D
Review paper

VARIABILITY OF FACTORS THAT AFFECT AVAILABILITY OF IRON, MANGANESE AND ZINC IN MAIZE LINES

Vesna DRAGIČEVIĆ¹, Snežana MLADENOVIĆ DRINIĆ¹, Milovan STOJILJKOVIĆ², Milomir FILIPOVIĆ¹, Zoran DUMANOVIĆ¹, Dragan KOVAČEVIĆ¹

¹ Maize Research Institute “Zemun Polje”, Zemun Polje

² Vinča Institute of Nuclear Sciences, Belgrade, Serbia

Abstract

Dragičević V, S. Mladenović Drinić, M. Stojiljković, M.Filipović, Z. Dumanović and D. Kovačević (2013): Variability of factors that affect availability of iron, manganese and zinc in maize lines. Genetika, Vol 45, No. 3, 907-920

Deficiencies of some mineral elements are causing serious health problems, which could be prevented by increase of mineral nutrients in food with supplementation, food fortification or plant breeding. From this point, experiment with 78 maize inbred lines was set up to determine maize lines with improved contents of Fe, Zn and Mn, as well as their relations with phytic acid, inorganic phosphorus and β-carotene, as factors which affect their absorption. Obtained results suggest that investigated maize lines show high variability in concentration of Fe, Mn and Zn, as well as phytic acid (which sustain availability of mineral elements) and β-carotene (which enables better absorption of mineral element and minimize negative effect of phytic acid). From this point of view, group of genotypes with phytic P ≤ 3 g kg^-1 was interesting. Among them, L2 and L23 are maize lines with relatively high inorganic P, Fe and Zn contents, and together with relatively low ratio between phytic and inorganic P, they could be used as a good source of P, Fe and Zn. On the other hand, L1 and L4 are also maize lines with high inorganic P, β-carotene and Mn, and favourable ratio between phytic acid and Fe and Zn, what could give them advance as source of Mn in breeding programs. The same maize lines could also be candidates with improved ability for Fe and Zn absorption, what is based on high β-carotene content. Maize line L14, with relatively high concentration of all three

Key words: maize lines, microelements, phytic acid, β-carotene

Corresponding author: Vesna Dragićević, Maize Research institute, Slobodna Bajica 1, 11185 Zemun, Belgrade email: vdragicevic@mrizp.rs Phone +381113756704, Fax: +381113756707

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Published in „GENETIKA“ Vol. 45, No. 3(2013), pp. 921-928

Serbia

UDC 575:633.15
DOI: 10.2298/GENSR1303921B
Review paper

STALK ROT RESISTANCE IN MAKSIMIR 3 SYNTHETIC MAIZE POPULATION AFTER FOUR CYCLES OF RECURRENT SELECTION

Miroslav BUKAN¹, Hrvoje ŠARČEVIĆ¹, Ivica BUHINIČEK², Branko PALAVERŠIĆ², Ramsey S. LEWIS³, Vinko KOZUMPLIK¹

¹University of Zagreb, Faculty of Agriculture, Zagreb, Croatia; ²Bc Institute for Breeding and Production of Field Crops, Rugvica, Croatia, ³Department of Crop Science, North Carolina State University, Raleigh, NC 27695, USA

Abstract

Bukan M., H. Šarčević, I. Buhiniček, B. Palaveršić, R. Lewis, and V. Kozumplik (2013): Stalk rot resistance in maksimir 3 synthetic maize population after four cycles of recurrent selection. Genetika, Vol 45, No. 3, 921-928

Fusarium stalk rot (FSR) and anthracnose stalk rot (ASR), caused by Fusarium spp. and Colletotrichum graminicola (Ces.) G.W. Wils. respectively, are the two most important stalk diseases in maize which increase the incidence of stalk lodging and reduce grain yield. The aim of the present study was to (1) evaluate the effect of four cycles of recurrent selection in the Maksimir 3 Synthetic (M3S) maize population on ASR and FSR resistance and (2) to investigate the correlation among the different disease rating methods. The experiment included six M3S cycle populations per se and their test-crosses with a single cross hybrid. ASR resistance was estimated on artificially inoculated plant rows using three ratings (the number of infected internodes, the number of internodes rotten more than 75% and evaluation of outer stalk discoloration) whereas FSR resistance was estimated in artificially inoculated rows as well as in naturally inoculated rows by rating severity of disease symptoms on longitudinally cut stalks using the standard resistance scale. The results of the present study showed that four cycles of selection in the M3S maize population, conducted primarily for grain yield improvement, did not significantly affect its resistance to both ASR and FSR. Among the disease ratings a moderate positive correlation was found only between two ASR resistance ratings (the number of infected internodes and the number of internodes rotted more than 75%) in both population per se (r=0.49**) and population test-crosses (r=0.56**).

Key words: Colletotrichum graminicola (Ces.) G.W. Wils., Fusarium graminicola Schwabe, maize, recurrent selection, stalk rot

Corresponding author: Miroslav Bukan, University of Zagreb, Faculty of Agriculture, Zagreb, Croatia

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Published in „GENETIKA“ Vol. 45, No. 3(2013), pp. 929-938

Serbia

UDC 575
DOI: 10.2298/GENSR1303929K
Review paper

TOXICOLOGY AND CYTOGENETIC ANALYSIS OF A Drosophila melanogaster MUTANT RESISTANT TO IMIDACLOPRID AND DDT

Predrag KALAJDZIC^1,2,ⁿ, Stefan OEHLER^3,#, Maria MARKAKI⁴, Charalambos SAVAKIS^2,3

¹Institute for Biological Research “Sinisa Stankovic”, University of Belgrade, Belgrade, Serbia,

²Medical School, University of Crete, Heraklion, Greece

³Institute of Cellular and Developmental Biology, Biomedical Sciences Research Center “Alexander Fleming”, Varkiza, Greece

⁴Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology – Hellas, Heraklion, Crete, Greece

Current address:

#Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, New Delhi, India

ⁿMax-Planck Institute for Evolutionary Biology, Plön, Germany

Abstract

Kalajdzic P., S. Oehler, M. Markaki, and C. Savakis (2013): Toxicology and cytogenetic analysis of a Drosophila melanogaster mutant resistant to imidacloprid and ddt. Genetika, Vol 45, No. 3, 929-938.

Resistance to all major insecticide classes has developed in numerous and diverse insect field populations. Imidacloprid, the worldwide most used neonicotinoid, has been extensively applied during the last decade for the control of different insect pests. Lately, cases of sporadic resistance also to neonicotinoids, including Imidacloprid, have been reported. Drosophila melanogaster is one of the most popular model organisms in biology and, although not a pest species, a promising model system for insecticide resistance research. In this study, we present a toxicological and karyotypic analysis of a Drosophila mutant (MiT[w^-]3R2) resistant to Imidacloprid and cross-resistant to DDT. Karyotype analysis of polytene chromosome of MiT[w^-]3R2 flies did not identify any apparent structural change of the polytene chromosome linked with the resistance phenotype.

Key words: Drosophila melanogaster, Imidacloprid, toxicology analysis, insecticide resistance

Corresponding author: Predrag Kalajdzic,Institute for Biological Research "Siniša Stankovic", University of Belgrade,142 Despot Stefan Blvd., 11060 Belgrade, Serbia,Tel.: +381 11 2078334,Fax: +381 11 2761433,Email: pedja@ibiss.bg.ac.rs

Max-Planck Institute for Evolutionary Biology, Plön, Germany,August-Thienemann-Str. 2, 24306 Plön, Telefon: +49 (4522) 763 – 0,Telefax: +49 (4522) 763 – 351,Email: pedja@evolbio.mpg.

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Published in „GENETIKA“ Vol. 45, No. 3(2013), pp. 939-952

Serbia

UDC 575:630
DOI: 10.2298/GENSR1303939M
Review paper

ASSESSMENT OF SELF-(IN) COMPATIBILITY IN SOME

SWEET CHERRY (Prunus avium L.) GENOTYPES

Sanja RADIČEVIĆ¹, Slađana MARIĆ¹, Radosav CEROVIĆ², Milena ĐORĐEVIĆ¹

¹Fruit Research Institute, Department of Pomology and Fruit Breeding, Čačak, Serbia

²Maize Research Institute „Zemun Polje”, Belgrade-Zemun, Serbia

Abstract

Radičević S., S. Marić, R. Cerović, and M. Đorđević (2013): Assessment of self-(in )compatibility in some sweet cherry (Prunus avium L.) genotypes. Genetika, Vol 45, No. 3, 939-952.

The paper presents results of a three-year study of self-(in)compatibility in four economically important sweet cherry genotypes – ‘Karina’, ‘Kordia’, ‘Regina’ and ‘Summit’, under agro-environmental conditions of Western Serbia. Determination of S-RNase genotype, microscopic observation of the pollen tube growth rate and assaying of the fruit set level after self-pollination were used to assess the genotypes. ‘Kordia’ (S₃S₆), ‘Regina’ (S₁S₃) and ‘Summit’ (S₁S₂) are self-incompatible genotypes, with a considerable number of pollen tubes ending the growth in the middle third of the style and lack of fruit set. ‘Karina’, as S₃S₄ genotype, behaved as self-compatible, since its pollen tubes reached the base of the style and ovary, penetrating the nucellus. In addition, fruit set for ‘Karina’ was recorded in all three years of study (40.26%, 18.79% and 21.81%, respectively).

Key words: Prunus avium; S-genotype; self-pollination; pollen tube growth; fruit set

Corresponding author: Sanja Radičević, Fruit Research Institute,Kralja Petra I 9,32000 Čačak, Serbia,Phone: 032 221 413,Fax: 032 221 391,E-mail: sanjaradicevic@yahoo.com

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Published in „GENETIKA“ Vol. 45, No. 3(2013), pp. 953-961

Serbia

UDC 575.630
DOI: 10.2298/GENSR1303953G
Review paper

GENETIC CHARACTERIZATION OF PATHOGENIC FLUORESCENT PSEUDOMONADS ISOLATED FROM NECROTIC CHERRY AND PLUM BUDS

IN SERBIA

Veljko GAVRILOVIĆ¹, Žarko IVANOVIĆ¹, Tatjana POPOVIĆ¹, Svetlana ŽIVKOVIĆ¹, Slaviša STANKOVIĆ², Tanja BERIĆ² and Đorđe FIRA^2*

¹Institute for Plant Protection and the Environment, Belgrade, Serbia

²Faculty of Biology, University of Belgrade, Belgrade, Serbia

Abstract

Gavrilović V., Ž. Ivanović, T. Popović, S. Živković, S.Stanković, T.Berić and Đ. Fira (2013): Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia. Genetika, Vol 45, No. 3, 953-961.

During past few years a symptoms of plum and cherry bud necrosis were observed in some regions with significant cherry production in Serbia. Gram negative, fluorescent, oxidative bacterial strains were isolated from the margin of necrotic tissue. All investigated strains are levan and HR positive, while negative results are recorded in oxidase, pectinase and arginin dihydrolase tests (LOPAT+- - - +). Symptoms similar to those observed in natural infection were obtained after artificial inoculation of cherry leaf scares and dormant one year old cherry shoots. Investigated strains as well as reference strain of P. syringae pv. morsprunorum cause the superficial necrosis on artificially inoculated immature cherry fruits, but negative results were recorded in immature pear and lemon fruit tests as well as syringae leaves and bean pods. Gelatin and aesculin tests were negative and tyrosinase and tartrate were positive. Investigated strains isolated from necrotic cherry buds had identical REP-PCR pattern with reference strain of P. syringae pv. morsprunorum. On the basis of obtained results, it was concluded that this bacterium is causal agent of cherry trees bud necrosis in Serbia.

Key words: bud necrosis, cherry, GATT, pathogenicity, P. syringae pv. morsprunorum, plum, REP-PCR

Corresponding author: Đorđe Fira,Faculty of Biology, University of Belgrade, Belgrade, Serbia,Tel/fax: +381 11 2639882, E-mail: fira@bio.bg.ac.rs

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Published in „GENETIKA“ Vol. 45, No. 3(2013), pp. 963-977

Serbia

UDC 575
DOI: 10.2298/GENSR13039963G
Review paper

MORPHOLOGICAL EVALUATION OF COMMON BEAN DIVERSITY IN BOSNIA AND HERZEGOVINA USING THE DISCRIMINANT ANALYSIS OF PRINCIPAL COMPONENTS (DAPC) MULTIVARIATE METHOD

Jasmin GRAHIĆ, Fuad GAŠI, Mirsad KURTOVIĆ, Lutvija KARIĆ, Mirha ĐIKIĆ, Drena GADŽO

Faculty of Agriculture and Food Sciences, University of Sarajevo, Sarajevo,

Bosnia and Herzegovina

Abstract

Grahić J., F. Gaši, M. Kurtović, L. Karić, M. Đikić and D. Gedžo (2013): Morphological evaluation of common bean diversity in Bosnia and Herzegovina using the discriminant analysis of principal components (DAPC) multivariate method Genetika, Vol 45, No. 3, 963-977.

In order to analyze morphological characteristics of locally cultivated common bean landraces from Bosnia and Herzegovina (B&H), thirteen quantitative and qualitative traits of 40 P. vulgaris accessions, collected from four geographical regions (Northwest B&H, Northeast B&H, Central B&H and Sarajevo) and maintained at the Gene bank of the Faculty of Agriculture and Food Sciences in Sarajevo, were examined. Principal component analysis (PCA) showed that the proportion of variance retained in the first two principal components was 54.35%. The first principal component had high contributing factor loadings from seed width, seed height and seed weight, whilst the second principal component had high contributing factor loadings from the analyzed traits seed per pod and pod length. PCA plot, based on the first two principal components, displayed a high level of variability among the analyzed material. The discriminant analysis of principal components (DAPC) created 3 discriminant functions (DF), whereby the first two discriminant functions accounted for 90.4% of the variance retained. Based on the retained DFs, DAPC provided group membership probabilities which showed that 70% of the accessions examined were correctly classified between the geographically defined groups. Based on the taxonomic distance, 40 common bean accessions analyzed in this study formed two major clusters, whereas two accessions Acc304 and Acc307 didn’t group in any of those. Acc360 and Acc362, as well as Acc324 and Acc371 displayed a high level of similarity and are probably the same landrace. The present diversity of Bosnia and Herzegovina’s common been landraces could be useful in future breeding programs.

Key words: common bean landraces, morphological traits, principal component analysis, discriminant analysis of principal components, hierarchical clustering

Corresponding author: Jasmin Grahić, Faculty of Agriculture and Food Sciences, University of Sarajevo, Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and Herzegovina, tel+387 33 225 727, Fax number: +387 33 667 429,E mail: grahic.jasmin@live.com

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