GENETIKA, Vol. 46, No. 2 (2014)

Dragana MILADINOVIĆ, Ksenija TAŠKI-AJDUKOVIĆ, Nevena NAGL, Branislav KOVAČEVIĆ, Aleksandra DIMITRIJEVIĆ, Ivana IMEROVSKI, Jegor MILADINOVIĆ, Nenad DUŠANIĆ, Igor BALALIĆ

GENETIC DIVERSITY OF WILD SUNFLOWER (Helianthus sp.) ACCESSIONS WITH DIFFERENT TOLERANCE TO MID-STALK WHITE ROT [Abstract] [Full text]

Antun JAMBROVIĆ, Maja MAZUR, Zvonko RADAN, Zvonimir ZDUNIĆ, Tatjana LEDENČAN, Andrija BRKIĆ, Josip BRKIĆ, Ivan BRKIĆ, and Domagoj ŠIMIĆ

ARRAY-BASED GENOTYPING AND GENETIC DISSIMILARITY ANALYSIS OF A SET OF MAIZE INBRED LINES BELONGING TO DIFFERENT HETEROTIC GROUPS

[Abstract] [Full text]

Ivan MILENKOVIĆ, Justyna A. NOWAKOWSKA, Tomasz OSZAKO, Katarina MLADENOVIĆ, Aleksandar LUČIĆ, Ljubinko RAKONJAC, Dragan KARADŽIĆ

MORPHOLOGICAL AND MOLECULAR IDENTIFICATION OF Phytophthora SPECIES FROM MAPLE TREES IN SERBIA [Abstract] [Full text]

Reza DARVISHZADEH, Hamid HATAMI MALEKI, Alireza PIRZAD, Maryam KHOLGHIand Babak ABDOLLAHI MANDOULAKANI

GENETIC ANALYSIS OF YIELD AND YIELD RELATED TRAITS IN SUNFLOWER (Helianthus annuus L.) UNDER WELL-WATERED AND WATER-STRESSED CONDITIONS [Abstract] [Full text]

Miodrag Dimitrijević and Sofija Petrović

Species variation of Aegilops genus and heavy metal content in plant habitat soil at Southern Adriatic localities [Abstract] [Full text]

Hemant Rakhamaji ADSUL and Bachubhai Arjanbhai MONPARA

GENETIC DIVERGENCE ANALYSIS IN INDIGENOUSLY DEVELOPED INDIAN SOYBEAN (Glycine max L. Merrill) GERMPLASM [Abstract] [Full text]

Milena ĐORĐEVIĆ, Radosav CEROVIĆ, Sanja RADIČEVIĆ, Dragan NIKOLIĆ

INCOMPATIBLE POLLEN TUBES IN THE PLUM STYLE AND THEIR IMPACT ON FERTILIZATION SUCCESS [Abstract] [Full text]

Abdollah GHASEMI PIRBALOUTI

DIVERSITY IN CHEMICAL COMPOSITION AND YIELD OF ESSENTIAL OIL FROM TWO IRANIAN LANDRACES OF SWEET BASIL [Abstract] [Full text]

Vučeta Jaćimović, and Djina Božović

BioloGICAL traits OF cORNELIAN CHERRY GENOTyPES (Cornus mas L.) FROM TERITORY OF MONTENEGRO [Abstract] [Full text]

Nasim SOHRABIFAR, Jalal GHARESOURAN,Mahnaz TALEBI, Morteza GHOJAZADEH, Seiied Mojtaba MOHADDES ARDEBILI

ESTROGEN RECEPTOR ALPHA GENE POLYMORPHISMS IN PATIENTS WITH LATE ONSET ALZHEIMER’S DISEASE [Abstract] [Full text]

Milan MIROSAVLJEVIĆ, Novo PRŽULJ, Jan BOĆANSKI, Dušan STANISAVLJEVIĆ, Bojan MITROVIĆ

THE APPLICATION OF AMMI MODEL FOR BARLEY CULTIVARS EVALUATION IN MULTI-YEAR TRIALS [Abstract] [Full text]

Milda RUDZIANSKIENE, Arturas INCIURA, Elona JUOZAITYTE, Rolandas GERBUTAVICIUS, Renata SIMOLIUNIENE, Rasa UGENSKIENE, Danguole RAULINAITYTE, Viktoras RUDZIANSKAS, Greta Emilia KIAVIALAITIS

THE ROLE OF SINGLE NUCLEOTIDE POLYMORPHISM OF IL-6 AND IL-10 CYTOKINE ON PAIN SEVERITY AND PAIN RELIEF AFTER RADIOTHERAPY IN MULTIPLE MYELOMA PATIENTS WITH PAINFUL BONE DESTRUCTIONS [Abstract] [Full text]

Mirjana PETROVIĆ, Zora DAJIĆ – STEVANOVIĆ, Dejan SOKOLOVIĆ, Jasmina RADOVIĆ, Jasmina MILENKOVIĆ, Jordan MARKOVIĆ

STUDY OF RED CLOVER WILD POPULATIONS FROM THE TERRITORY OF SERBIA FOR THE PURPOSE OF PRE-SELECTION [Abstract] [Full text]

Stanislava GROZEVA and Nikolay VELKOV

IN VITRO PLANT REGENERATION OF TWO CUCUMBER (Cucumis sativum L.) GENOTYPES: EFFECTS OF EXPLANT TYPES AND CULTURE MEDIUM [Abstract] [Full text]

Milka BRDAR-JOKANOVIĆ, Zdenka GIREK, Suzana PAVLOVIĆ, Milan UGRINOVIĆ, Jasmina ZDRAVKOVIĆ

SHOOT AND ROOT DRY WEIGHT IN DROUGHT EXPOSED TOMATO POPULATIONS

[Abstract] [Full text]

M. Kadri BOZOKALFA,Bülent YAĞMUR Dursun EŞİYOK, Tansel KAYGISIZ AŞÇIOĞUL

GENETIC VARIABILITY AND ASSOCIATION PATTERN AMONG QUANTITATIVE NUTRITIONAL TRAITS IN SWISS CHARD (Beta vulgaris subsp. L. var. cicla) ACCESSIONS AND ITS IMPLICATION FOR BREEDING [Abstract] [Full text]

Rosaiah KOTIKALAPUDI,Rajesh Kumar PATEL, Richa Singh KUSHWAH and Phani Sri Satish SUNKARA

IDENTIFICATION OF CITRULLINAEMIA CARRIER AND DETECTION OF A NEW SILENT MUTATION AT 240bp POSITION IN ASS1 GENE OF NORMAL HOLSTEIN CATTLE [Abstract] [Full text]

Lotan Kumar BOSE, Nitiprasad Namdeorao JAMBHULKAR and Kanailal PANDE

GENOTYPE BY ENVIRONMENT INTERACTION AND STABILITY ANALYSIS FOR RICE GENOTYPES UNDER BORO CONDITION [Abstract] [Full text]

Nevena H. DJUKIĆ, Desimir S. KNEŽEVIĆ

MOLECULAR CHARACTERIZATION AND GENETIC DIVERSITY ANALYSIS β-GLUCAN CONTENT VARIABILITY IN GRAIN OF OAT (Avena sativa L.)

[Abstract] [Full text]

Mahwish KANWAL, FARHATULLAH, M. Ashiq RABBANI, Sidra IQBAL, Laila FAYYAZ and M. AFZAL

GENETIC DIVERSITY IN BRASSICA SPECIES AND ERUCA SATIVA FOR YIELD ASSOCIATED PARAMETERS [Abstract] [Full text]

Mirjana JANKULOVSKA, Sonja IVANOVSKA, Ana MARJANOVIC-JEROMELA, Snjezana BOLARIC, Ljupcho JANKULOSKI, Zoran DIMOV, Dane BOSEV, Biljana KUZMANOVSKA

MULTIVARIATE ANALYSIS OF QUANTITATIVE TRAITS CAN EFFECTIVELY CLASSIFY RAPESEED GERMPLASM [Abstract] [Full text]

Milena CVETKOVIĆ, Stevo NAJMAN, Miloš NIKOLIĆ

SECULAR CHANGES IN CEPHALIC INDEX-A STUDY OF SERBIAN SCHOOL CHILDREN

[Abstract] [Full text]

Krishna Nand MAURYA, Sudhir SHUKLA and Geeta ASTHANA

PATTERN OF QUANTITATIVE INHERITANCE OF YIELD AND COMPONENT TRAITS IN OPIUM POPPY (Papaver somniferum L.) [Abstract] [Full text]

Marija KOSTADINOVIC, Dragana IGNJATOVIC-MICIC, Goran STANKOVIC, Jelena VANCETOVIC, Danijela RISTIC, Snezana MLADENOVIC DRINIC

GENETIC AND BIOCHEMICAL CHARACTERISATION OF PARENTAL INBRED LINES IN MARKER ASSISTED SELECTION FOR QUALITY PROTEIN MAIZE

[Abstract] [Full text]

Mehrangiz FATHI, Ghorban ELYASI ZARRINGHOBAIE

ASSOCIATION OF POLYMORPHISMS IN THE PROMOTER REGION OF TURKEY PROLACTIN WITH EGG PERFORMANCE [Abstract] [Full text]

Ivana ILIĆ DIMITRIJEVIĆ_,Thomas LÖNING_,Marcus FALK,Vitomir S. KONSTANTINOVIĆ_,Miroslav VUKADINOVIĆ, Zvezdana TEPAVČEVIĆ_,Saša TABAKOVIĆ_,Nataša PEJČIĆ, Biljana MILIČIĆ, Jelena MILAŠIN

INCIDENCE AND CLINICAL RELEVANCE OF T(11;19) TRANSLOCATION IN SALIVARY GLAND MUCOEPIDERMOID CARCINOMA [Abstract] [Full text]

Jalal GHARESOURAN, Maryam REZAZADEH, Morteza GHOJAZADEH, Mojtaba MOHADDES ARDABILI

MUTATION SCREENING OF FAMILIAL MEDITERRANEAN FEVER IN THE AZERI TURKISH POPULATION: GENOTYPE–PHENOTYPE CORRELATION AND THE CLINICAL PROFILE VARIABILITY [Abstract] [Full text]

Ljudmila NAGORNI-OBRADOVIĆ, Nela MAKSIMOVIĆ, Predrag MILJIĆ, Dragana CVETKOVIĆ, Ruža STEVIĆ, Dragica PEŠUT

PHENOTYPIC PRESENTATION OF THROMBOPHILIA IN DOUBLE HETEROZYGOTE FOR FACTOR V LEIDEN AND PROTHROMBIN 20210 G>A MUTATIONS – CASE REPORT [Abstract] [Full text]

Kuo GAO, Meiying NIU, Xing ZHAI, Youliang HUANG, Xin TIAN, Tiangang LI

GENETIC AND NON-GENETIC FACTORS RESPONSIBLE FOR MITOCHONDRIAL FAILURE AND ALZHEIMER’S DISEASE [Abstract] [Full text]

Published in „GENETIKA“ Vol. 46, No. 2 (2014), pp. 331-342

UDC 575:633
DOI: 10.2298/GENSR1402331M

Original scientific paper

GENETIC DIVERSITY OF WILD SUNFLOWER (Helianthus sp.) ACCESSIONS WITH DIFFERENT TOLERANCE TO MID-STALK WHITE ROT

Dragana MILADINOVIĆ¹, Ksenija TAŠKI-AJDUKOVIĆ¹, Nevena NAGL¹, Branislav KOVAČEVIĆ², Aleksandra DIMITRIJEVIĆ¹, Ivana IMEROVSKI¹, Jegor MILADINOVIĆ¹, Nenad DUŠANIĆ¹, Igor BALALIĆ¹

¹Institute of Field and Vegetable Crops, Novi Sad, Serbia

²University of Novi Sad, Institute of Lowland Forestry and Environment, Novi Sad, Serbia

Abstract

Miladinović D., K. Taški-Ajduković, N. Nagl, B. Kovačević, A. Dimitrijević, I. Imerovski, J. Miladinović, N. Dušanić, I.Balalić (2014): Genetic diversity of wild sunflower (Helianthus sp.) accessions with different tolerance to mid-stalk white rot- Genetika, vol., No.46, No.2, 331-342.

Random amplified polymorphic DNA (RAPD) markers were used to detect polymorphism among accessions of wild sunflower species Hеlianthus maximiliani, Helianthus tuberosus, Helianthus mollis and Helianthus rigidus with different tolerance to mid-stalk white rot and selection of potential markers for different levels of tolerance to this disease. Estimates of genetic variation showed that genetic diversity was equally distributed between Helianthus species and within them. Cluster analysis corresponded to the phylogenetic relations within the genus Helianthus. The results obtained by principal coordinates analysis (PCoA), where the first two principal coordinates accounted for 83.7% of total variation, perfectly coincided with the results of cluster analysis. Contingency coefficient significance test showed that most of the used primers generated bands associated with some level of tolerance or susceptibility to mid-stalk white rot. Furthermore, contingency analysis showed that primer C12 generated bands associated with resistance (100%) to mid-stalk white rot both in H. mollis and in all accessions, while primer X18 generated bands significantly associated with high tolerance (75%) in H. rigidus, H. mollis as well as in all tested accessions. The C15-600 bp locus was found to be significantly associated with high tolerance (75%) in all accessions, and medium tolerance (50%) in H. mollis.

Key words: breeding, contingency test, Helianthus, multivariate analysis, RAPD markers, Sclerotinia sclerotiorum

Corresponding author: Dragana Miladinović, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia; Phone: +381214898421; Fax: +381216413833; E-mail: dragana.miladinovic@ifvcns.nc.ac.rs

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Published in „GENETIKA“ Vol. 46, No. 2 (2014), pp. 343 -352

Serbia

UDC 575:633.15
DOI:10.2298/GENSR1402343J

Original scientific paper

ARRAY-BASED GENOTYPING AND GENETIC DISSIMILARITY ANALYSIS

OF A SET OF MAIZE INBRED LINES BELONGING

TO DIFFERENT HETEROTIC GROUPS

Antun JAMBROVIĆ¹, Maja MAZUR¹, Zvonko RADAN², Zvonimir ZDUNIĆ¹, Tatjana LEDENČAN¹, Andrija BRKIĆ¹, Josip BRKIĆ¹, Ivan BRKIĆ¹, and Domagoj ŠIMIĆ¹

¹Agricultural Institute Osijek, Osijek, Croatia

²Fermopromet d.o.o., Novi Bezdan, Baranjsko Petrovo Selo, Croatia

Abstract

Jambrović A., M. Mazur, Z. Radan, Z. Zdunić, T. Ledenčan, A. Brkić, J. Brkić, I. Brkić, and D. Šimić (2014): Array-based genotyping and genetic dissimilarity analysis of a set of maize inbred lines belonging to different heterotic groups -Genetika, Vol 46, No. 2, 343-352.

Here we describe the results of the detailed array-based genotyping obtained by using the Illumina MaizeSNP50 BeadChip of eleven inbred lines belonging to different heterotic groups relevant for maize breeding in Southeast Europe - European Corn Belt. The objectives of this study were to assess the utility of the MaizeSNP50 BeadChip platform by determining its descriptive power and to assess genetic dissimilarity of the inbred lines. The distribution of the SNPs was found not completely uniform among chromosomes, but average call rate was very high (97.9%) and number of polymorphic loci was 33200 out of 50074 SNPs with known mapping position indicating descriptive power of the MaizeSNP50 BeadChip. The dendrogram obtained from UPGMA cluster analysis as well as principal component analysis (PCA) confirmed pedigree information, undoubtedly distinguishing lines according to their background in two population varieties of Reid Yellow Dent and Lancaster Sure Crop. Dissimilarity analysis showed that all of the inbred lines could be distinguished from each other. Whereas cluster analysis did not definitely differentiate Mo17 and Ohio inbred lines, PCA revealed clear genetic differences between them. The studied inbred lines were confirmed to be genetically diverse, representing a large proportion of the genetic variation occurring in two maize heterotic groups.

Key words: dissimilarity, inbred lines, maize, single nucleotide polymorphism (SNP), whole-genome genotyping

Corresponding author: Domagoj Šimić, Agricultural Institute Osijek, Južno predgrađe 17, HR-31103 Osijek, Croatia; Phone: ++385 31 515 521, e-mail: domagoj.simic@poljinos.hr

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Published in „GENETIKA“ Vol. 46, No. 2 (2014), pp. 353- 368

Serbia

UDC 575
DOI: 10.2298/GENSR1402353M

Original scientific paper

MORPHOLOGICAL AND MOLECULAR IDENTIFICATION OF Phytophthora SPECIES FROM MAPLE TREES IN SERBIA

Ivan MILENKOVIĆ¹⁾, Justyna A. NOWAKOWSKA²⁾, Tomasz OSZAKO²⁾, Katarina MLADENOVIĆ¹⁾, AleksandaR LUČIĆ¹⁾, Ljubinko RAKONJAC¹⁾, Dragan KARADŽIĆ³⁾

¹Institute of Forestry, , Belgrade, Serbia

²Forest Research Institute-IBL, Sekocin Stary, Raszyn, Poland

³University of Belgrade-Faculty of Forestry, Belgrade, Serbia

Abstract

Milenković I., J. A. Nowakowska, T. Oszako, K. Mladenović, A. Lučić, L. Rakonjac, D. Karadžić (2014): Morphological and molecular identification of Phytophthora species from maple trees in Serbia. -Genetika, Vol 46, No. 2, 353-368.

The paper presents the results of the study performed with aims to determine the presence and diversity of Phytophthora species on maple trees in Serbia. Due to high aggressiveness and their multicyclic nature, presence of these pathogens is posing significant threat to forestry and biodiversity. In total, 29 samples of water, soil and tissues were taken from 10 different localities, and six different maple hosts were tested. After the isolation tests, 17 samples from five different maple hosts were positive for the presence of Phytophthora spp., and 31 isolates were obtained. After the detailed morphological and physiological classification, four distinct groups of isolates were separated. DNA was extracted from selected representative isolates and molecular identification with sequencing of ITS region was performed. Used ITS4 and ITS6 primers successfully amplified the genomic DNA of chosen isolates and morphological identification of obtained isolates was confirmed after the sequencing. Four different Phytophthora species were detected, including P. cactorum, P. gonapodyides, P. plurivora and P. lacustris. The most common isolated species was homothallic, and with very variable and semipapillate sporangia, P. plurivora with 22 obtained isolates. This is the first report of P. plurivora and P. gonapodyides on A. campestre, P. plurivora and P. lacustris on Acer heldreichii and first report of P. lacustris on A. pseudoplatanus and A. tataricum in Serbia.

Key words: Acer spp., morphology, Phytophthora plurivora, PCR, Sequencing

Corresponding author: Ivan Milenković, Institute of foresty, Kneza Višeslava 3, 11030 Belgrade Serbija, tel: +381 64 2031985; E-mail: ivan.milenkovic@sfb.bg.ac.rs

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Published in „GENETIKA“ Vol. 46, No.2 (2014), pp. 369 -384

Serbia

UDC 575:633
DOI: 10.2298/GENSR1402369D

Original scientific paper

GENETIC ANALYSIS OF YIELD AND YIELD RELATED TRAITS IN SUNFLOWER (Helianthus annuus L.) UNDER WELL-WATERED AND WATER-STRESSED CONDITIONS

Reza DARVISHZADEH^1,2, Hamid HATAMI MALEKI³, Alireza PIRZAD¹,

Maryam KHOLGHI^1,2and Babak ABDOLLAHI MANDOULAKANI^1,2

^{^[1]}Department of Plant Breeding and Biotechnology, Urmia University, Urmia, Iran.

²Institue of Biotechnology, Urmia University, Urmia, Iran.

³Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran

Abstract

Darvishzadeh R., H. Hatami Maleki, A. Pirzad, M. Kholghiand B. Abdollahi Mandoulakani (2014): Genetic analysis of yield and yield related traits in sunflower (Helianthus annuus L.) under well-watered and water-stressed conditions -. Genetika, Vol 46, No. 2, 369-384.

Drought stress is one of the factors which influence sunflower (Helianthus annuus L.) production. Breeding for tolerance to drought stress has become a major focus. In the present investigation, combining ability, gene action and genetic analysis of several characteristics were studied in six pure lines of sunflower and their 15 hybrids. The materials were evaluated in two separate experiments using a randomized complete block design (RCBD) with three replications in two states (well-watered and water-stressed) under controlled conditions. Comparison of mean values exhibited that under water-stressed condition the average performance of sunflower genotypes were decreased for all studied traits. In well-watered condition the highest value for seed yield per plant (SY) was observed in the cross 'LR4´LR25', whereas in water-stressed condition the highest value for this trait was observed in the hybrid 'C104´LR25'. Combining ability analysis revealed that most of agronomical traits such as head diameter, number of achene per head, head weight and seed yield inherited differently in stressed and non-stressed conditions. In water-stressed conditions, the non-additive effects played a more important role for controlling the number of achene per head (NA), seed yield per plant (SY), head diameter (HD), and days from flowering to physiological maturity (DFM) than additive. Based on results yield improvement for water-stressed conditions requires selection under drought conditions. In well-watered condition, the cross 'LR4´C10' showed the best SCA value for seed yield per plant (SY). In water-stressed conditions, 'RHA266´C100' had the highest SCA for seed yield per plant (SY) and number of achene (NA) per head.

Key words: additive effects, combined analysis of experiments, drought stress, diallel analysis, relative water content, recombinant inbred lines.

Corresponding author: Dr. R. Darvishzadeh, Department of Plant Breeding and Biotechnology, Urmia University, Urmia, Iran. E-mail: r.darvishzadeh@urmia.ac.ir. Phone: + 98 441 2972785. Fax: + 98 441 2779558

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Published in „GENETIKA“ Vol. 46, No.2 (2014), pp. 385 -400

Serbia

UDC 575:633
DOI: 10.2298/GENSR1402385D

Original scientific paper

Species variation of Aegilops genus and heavy metal content

in plant habitat soil at Southern Adriatic localities

Miodrag Dimitrijević^* and Sofija Petrović

University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia

Abstract

Dimitrijević M. and S. Petrović (2014): Species variation of Aegilops genus and heavy metal content in plant habitat soil at Southern Adriatic localities -. Genetika, Vol 46, No. 2, 385 - 400.

The Aegilops genus is a wild relative to the bread wheat, having chromosomes homologous to wheat chromosomes. That genus could be the source of many usefull abiotic stress tolerance genes. Facing a global climate changes, as well as, environmental erosion, it is important to create a desirable genetic variability that could correspond to environmental challenges. Heavy metals in soil could cause soil pollution, could lead to different phenotypic changes in plants, and could enter food chain. Assessment of Aegilops sp. population variation, as well as, heavy metal content in their habitat was the main goal in this research. Aegilops population composition was examined and samples were taken from 55 localities of South Adriatic coastal and littoral areas. Topsoil samples from all the localities were taken and heavy metal content, namely Cr, Pb, Zn, Ni, Cd and Cu, was analyzed,. Manganese content was measured, as well. Value of pH was established.

Key words: biodiversity, wild relatives, Aegilops, soil, heavy metal

Corresponding author: Miodrag Dimitrijević, Faculty of Agriculture, sq. D. Obradovića 8, 21000 Novi Sad, Serbia. Phone: +381 21 485 3211, e-mail mishad@polj.uns.ac.rs

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Published in „GENETIKA“ Vol. 46, No. 2 (2014), pp. 401 -409

Serbia

UDC 575:633
DOI: 10.2298/GENSR1402401A

Original scientific paper

GENETIC DIVERGENCE ANALYSIS IN INDIGENOUSLY DEVELOPED

INDIAN SOYBEAN (Glycine max L. Merrill) GERMPLASM

Hemant Rakhamaji ADSUL and Bachubhai Arjanbhai MONPARA

¹Department of Genetics and Plant Breeding, Junagadh Agricultural University, Junagadh- (Gujarat), India.

² Agricultural Research Station, Junagadh Agricultural University, Amreli (Gujarat), India.

Abstract

Adsul H. R. and B. A. Monpara (2014): Genetic divergence analysis in indigenously developed Indian soybean (Glycine max L. Merrill) germplasm.-Genetika, Vol 46, No. 2, 401-409.

Knowledge of the naturally occurring diversity helps to identify diverse groups of soybean genotypes that can be useful for the breeding program. Therefore, this study aims to identify traits that influence the soybean genotypes in cluster formation using D² analysis. Hundred genotypes of soybean were studied for 15 characters in randomized block design with three replications and grouped into fifteen clusters. The cluster I was the largest with 55 genotypes followed by cluster III containing 17 genotypes and cluster IV containing 16 genotypes. The remaining clusters were solitary with single genotype each. The inter-cluster distance was the highest between clusters XIII and XIV (D=38.28) followed by clusters X and XII (D=33.64), XIII and XIV (D=32.71), III and XIV (D=32.06) and XII and XIV (D=31.65). Genotypes falling in these clusters may serve as potential parents for a hybridization programme. Pods per plant contributed the highest in manifestation of total genetic diversity. The presence of clear phenotypic and genotypic differences in the characters under consideration between or among clusters gives us an opportunity to bring about improvement through hybridization of genotypes between these clusters and subsequent selection in the segregating generations. The genotype JS (SH) 131 of cluster XIV, J 606 of cluster X, JS 46-75 of cluster V and Himso 1548 of cluster XV were identified as genetically diverse parents, which can be utilized for future crop improvement programme.

Key words: D²statistics, genetic diversity, soybean germplasm

Corresponding author: Bachubhai Arjanbhai Monpara, Agricultural Research Station, Junagadh Agricultural University, Keriya Road, Amreli-365601 (Gujarat), India E-mail: bamonpara@yahoo.com

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Published in „GENETIKA“ Vol. 46, No. 2 (2014), pp.411 -418

Serbia

UDC 575.630
DOI: 10.2298/GENSR1402411D

Original scientific paper

INCOMPATIBLE POLLEN TUBES IN THE PLUM STYLE AND THEIR IMPACT ON FERTILIZATION SUCCESS

Milena ĐORĐEVIĆ¹, Radosav CEROVIĆ², Sanja RADIČEVIĆ¹, Dragan NIKOLIĆ³

¹Fruit Research Institute, Department of Pomology and Breeding, Čačak, Serbia

²Maize Research Institute “Zemun Polje”, Belgrade, Serbia

³University of Belgrade, Faculty of Agriculture, Belgrade, Serbia

Abstract

Đorđević M., R. Cerović, S. Radičević, D. Nikolić (2014): Incompatible pollen tubes in the plum style and their impact on fertilization success-. Genetika, Vol 46, No. 2, 411-418.

The pistils of plum (Prunus domestica L.) cv. ‘Čačanska Lepotica’ were self- and cross-pollinated in order to investigate occurrence of incompatible pollen tubes in the style, and their impact on fertilization success. The investigation also included open pollination variant. The highest percentage of these stopped the growth in the upper third of the style. Under cross-pollination variant, 15.4% and 12.1% of pollen tubes observed in the upper part of the style in the first and the second years of study, respectively, were found incompatible. In view of the above parameters, in the self-pollination variant, 15.0% and 17.0% of pollen tubes were found incompatible by years. As for the open pollination, percentages of incompatible pollen tubes in the upper part of the style by years were 14.0% and 14.4%, respectively. The occurrence of incompatible pollen tubes did not influence the fertilization success in these pollination variants.

Key words: fluorescence microscopy, incompatible pollen tubes, Prunus domestica

Corresponding author: Milena Đorđević, Fruit Research Institute, Kralja Petra I 9, 32000 Čačak, Serbia, Phone: 032 221 413, Fax: 032 221 391,E-mail: milena@ftn.kg.ac.rs

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Published in „GENETIKA“ Vol. 46, No.2 (2014), pp. 419 -426

Serbia

UDC 575:630
DOI: :10.2298/GENSR1402419P

Original scientific paper

DIVERSITY IN CHEMICAL COMPOSITION AND YIELD OF ESSENTIAL OIL FROM TWO IRANIAN LANDRACES OF SWEET BASIL

Abdollah GHASEMI PIRBALOUTI

Research Center for Medicinal Plants & Ethno-veterinary, Department of Medicinal Plants, Shahrekord Branch, Islamic Azad ‎University‏‎, Shahrekord, Iran

Abstract

Pirbalouti Ghasemi A. (2014): Diversity in chemical composition and yield of essential oil from two Iranian landraces of sweet basil.- Genetika, Vol 46, No. 2, 419-426.

Ocimum basilicum L. belongs to the family Lamiaceae is an herb that is extensively cultivated in some countries. Areal parts, especially leaves of sweet basil are widely used to enhance the flavour of foods such as salads, pasta, tomato products, vegetables, pizza, meat, soups, marine foods, confectioneries and other products. Essential oil yield and chemical components of two Iranian landraces of sweet basil including “Purple” and “Green” grown south-central of Iran (Isfahan province) were investigated. The hydro-distillated oils were analyzed by GC–MS. The oil yields were obtained from the aerial of Purple with 0.56 ml / 100 g dry matter and the aerial of Green with 0.48 ml / 100 g dry matter. Results indicated significant differences (p < 0.01) among the aerial for the main constituents in the essential oil from two Iranian landraces of sweet basil. The major constituents of the essential oil from the aerial of Purple landrace were methyl chavicol or estragol (63.32%) and linalool (7.96%). The main compositions of the essential oil from the aerial of Green landrace were methyl chavicol (31.82%), geranial (24.60%) and neral (22.65%). Genarlly, a comparison of our results with the previous reports suggests differences in the essential oil compositions and oil yield of the plant material could be attributed to genetic diversity in two Iranian landraces of sweet basil.

Key words: genetic diversity, geranial and ,neral essential oil, methyl chavicol, Ocimum basilicum L.

Corresponding author: Abdollah Ghasemi Pirbalouti ,Research Center for Medicinal Plants & Ethno-veterinary, Department of Medicinal Plants, Shahrekord Branch, Islamic Azad ‎University, PO. Box: ‎‏166‏‎, Shahrekord, Iran, E-mail: ghasemi@iaushk.ac.ir or aghasemipir@psis.umass.edu ,Tel: +98 (381) 336 10 87, Fax: +98 (381) 336 10 60

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Published in „GENETIKA“ Vol. 46, No.2 (2014), pp. 427-436

Serbia

UDC 575:630
DOI: 10.2298/GENSR1402427J

Original scientific paper

BioloGICAL traits OF cORNELIAN CHERRY GENOTyPES

(Cornus mas L.) FROM TERITORY OF MONTENEGRO

Vučeta Jaćimović, and Djina Božović

The Centre for Temperate Fruits Medical and Aromatic Herbs,

Bijelo Polje, Montenegro

Biotechnical faculty – Podgorica, Montenegro

Abstract

Jaćimović V., and Dj. Božović (2014): Biological traits of cornelian cherry genotypes (Cornus mas L.) from teritory of Montenegro.- Genetika, Vol 46, No. 2, 427-436.

In this paper are presented three years data (2009-2011) of biological traits of 30 cornelian cherry genotypes selected from the natural population of the region Upper Polimlje in Montenegro. Time of maturity, cropping, fruit and stone weight, size, fruit shape index and peel colour, content of flesh in total fruit mass and the soluble solids continent are presented. The aim of this paper is selection and preservation of cornelian cherry genotypes from natural population of Upper Polimlje that deserve attention due to their biological traits and recommendation of these genotypes for future propagation in present agricultural conditions or as a starting material in breeding of this fruit species.

Genotypes BA 70, BP 04, BP 17 and BP 44 with high cropping coefficient from 0.34 to 0.50 are worthy of attention as a big biological potential. For table consumption genotypes BA 70 and BP 21 are interesting, with fruit weight above 4 g, and genotypes PL 98, BP 44, PL 99, BP 36 and BP 22 with content of flesh in total fruit mass above 89% for processing. High content of soluble solids in the fruit, above 19%, are characteristic for genotypes BP 01, BP 06, BA 13, BP 38 and BP 40.

Key words: biological traits, Cornelian cherry, Montenegro, natural populations

Corresponding author: Božović Djina, The Centre for Temperate Fruits Medical and Aromatic Herbs, Bijelo Polje. Ul. Milorada Šćepanovića bb., 84000 Bijelo Polje. +38269306328, email: bdjina@yahoo.com

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Published in „GENETIKA“ Vol. 46, No. 2 (2014), pp. 437 - 444

Serbia

UDC 575

DOI: 10.2298/GENSR1402437S

Original scientific paper

ESTROGEN RECEPTOR ALPHA GENE POLYMORPHISMS IN PATIENTS

WITH LATE ONSET ALZHEIMER’S DISEASE

Nasim SOHRABIFAR¹, Jalal GHARESOURAN¹,Mahnaz TALEBI ²,

Morteza GHOJAZADEH³, Seiied Mojtaba MOHADDES ARDEBILI¹

¹Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

² Neurosciences research center, Tabriz University of Medical Sciences, Tabriz, Iran

³Liver and gastrointestinal disease research center, Tabriz University of Medical Sciences, Tabriz, Iran

Abstract

Sohrabifar N., J. Gharesouran,M. Talebi, M. Ghojazadeh, S. Mojtaba Mohaddes Ardebili (2014): Estrogen receptor alpha gene polymorphisms in patients withlate onset Alzheimer’s disease.- Genetika, Vol 46, No. 2, 437-444.

Evidences have been gathered from several studies suggest that a mechanism involving an estrogen-signaling pathway may contribute to modulate risk for Alzheimer’s disease. It was demonstrated that estrogen up-regulates the expression of apolipoprotein E gene, which has a role in the metabolism of -amyloid that is related to the progress of Alzheimer’s disease. Case-control studies have found an increased frequency of PvuII and XbaI polymorphisms in affected subjects. In this study we explore the possible association of different polymorphic forms of human -estrogen receptor (ER-a) with the risk to late onset Alzheimer’s disease in north-west Iranian population.

We conducted a case-control study in a dataset of 160 LOAD patients and 163 healthy controls that have been matched in gender and age. To evaluate the PvuII and XbaI polymorphisms in Alzheimer’s disease we used PCR/RFLP method and genotype frequencies were statistically determined. The PCR products prepared from 21 AD cases and 20 healthy controls were randomly purified by ethanol precipitation and bidirectionally sequenced.

The frequency of normal and mutated alleles for PvuII and XbaI locuses respectively, in the LOAD group were significantly higher than those in the control group (P<0.001, OR=0.51, 95 % CI 0.35-0.74 for XbaI locus; P<0.001, OR=0.41, 95 % CI 0.3-0.57 for PvuII locus). This result suggests that ERα XbaI and PvuII polymorphism is an additional risk factor for late-onset Alzheimer’s disease.

Key words: Alzheimer’s disease, -estrogen receptor, polymorphism, North-west of Iran

Corresponding author: Jalal Gharesouran, Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran., E-mail: Jalal.Gharesouran@gmail.com, Tel: 0098 337 15 87, Fax: 0098 337 15 87

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Published in „GENETIKA“ Vol. 46, No. 2 (2014), pp. 445 - 454

Serbia

UDC 575:633

DOI: 10.2298/GENSR1402445M

Original scientific paper

THE APPLICATION OF AMMI MODEL FOR BARLEY CULTIVARS EVALUATION IN MULTI-YEAR TRIALS

Milan MIROSAVLJEVIĆ¹, Novo PRŽULJ¹, Jan BOĆANSKI ², Dušan STANISAVLJEVIĆ¹, Bojan MITROVIĆ¹

¹ Institute of Field and Vegetable crops, Novi Sad

² University of Novi Sad, Faculty of Agriculture, Department of Field and Vegetable Crops

Abstract

Mirosavljević M., N. Pržulj, J. Boćanski, D. Stanisavljević, B. Mitrović (2014): The application of AMMI model for barley cultivars evaluation in multi-year trials-. Genetika, Vol 46, No. 2, 445-454.

The interpretation of new varieties performance is disturbed under the influence of genotype-by-environment interaction. Among several methods used for understanding this effect, one of the most frequently used methods is Additive Main Effects and Multiplicative Interaction (AMMI) analysis. In this study we used AMMI method with the aim to estimate the genotype - environment interaction of 14 barley genotypes, and to identify barley genotypes that have high and stable performance in different environments. The trials were conducted during 11 growing seasons (1995/96 - 2005/06), arranged in a randomized complete block (RCB) design with four replications in location Rimski Šančevi. The results showed that the influence of environment (seasons), genotypes and their interaction on barley grain yield were significant (p < 0.01). Based on AMMI method, two-rowed variety Novosadski 317 and the six-rowed variety Novosadski 331 can be distinguished due their high and stable yields.

Key words: AMMI analysis, Hordeum vulgare L., Genotype by environment interaction, Grain yield.

Corresponding author: Milan Mirosavljević, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad; Phone: 0214898220; Fax: 021 4898222; E-mail: milan.mirosavljevic@nsseme.com

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Published in „GENETIKA“ Vol. 46, No.2 (2014), pp. 455 -469

Serbia

UDC 575
DOI: 10.2298/GENSR1402455R

Original scientific paper

THE ROLE OF SINGLE NUCLEOTIDE POLYMORPHISM OF IL-6 AND IL-10 CYTOKINE ON PAIN SEVERITY AND PAIN RELIEF AFTER RADIOTHERAPY IN MULTIPLE MYELOMA PATIENTS WITH PAINFUL BONE DESTRUCTIONS

Milda RUDZIANSKIENE¹, Arturas INCIURA¹, Elona JUOZAITYTE¹, Rolandas GERBUTAVICIUS¹, Renata SIMOLIUNIENE², Rasa UGENSKIENE¹, Danguole RAULINAITYTE¹, Viktoras RUDZIANSKAS¹, Greta Emilia KIAVIALAITIS³

¹Oncology Institute of Lithuanian University of Health Sciences, Kaunas, Lithuania

²Department of Physics, Mathematics and Biophysics of Lithuanian University of Health Sciences, Kaunas, Lithuania

³Department of Anesthesiology, University Hospital Aachen, Aachen, Germany

Abstract

Rudzianskiene M., A. Inciura, E. Juozaityte, R. Gerbutavicius, R. Simoliuniene, R. Ugenskiene, D. Raulinaityte, V. Rudzianskas, G. E-Kiavialaitis (2014): The role of single nucleotide polymorphism of Il-6 and Il-10 cytokine on pain severity and pain relief after radiotherapy in multiple myeloma patients with painful bone destructions-. Genetika, Vol 46, No. 2, 455-469.

Multiple myeloma (MM) cells interact with bone marrow stromal cells stimulating transcription and secretion of cytokines like IL-6 and IL-10, which are implicated in the progression and dissemination of MM. Regulation of cytokines secretion is under genetic control through genetic polymorphisms in their coding and promoter sequences. It seems that single nucleotide polymorphism (SNP) in the promoter region of various genes may regulate the plasma concentrations of cytokines. Cytokines could be also hypothesized to function as pain modulators as peripheral nociceptors are sensitized by cytokines. The aim was to determine if the SNP of IL-6 and IL-10 cytokines could influence the analgesic response of radiotherapy in the treatment of painful bone destructions in MM patients. 30 patients (19 women and 11 men, median age: 67 years) with MM and painful bone destructions were treated with palliative radiotherapy. Pain was evaluated according to the visual analogue scale and analgesics intake. Pain scores and analgesics use were measured prior to radiotherapy as well as 4, 12 and 24 weeks afterward. Opioid analgesics were converted to the morphine-equivalent daily dose (MEDD). Genomic DNA was extracted from peripheral blood leukocytes and IL-6 and IL-10 gene promoter polymorphisms were analysed with polymerase chain reaction. 60% of patients reported severe pain prior to radiotherapy, which decreased to 13% at the first follow-up visit (p <0.001). The MEDD on admission to the hospital was 75 mg/day which decreased to 46 mg/day at the first follow-up visit (p = 0.033). A significant parameter in pain relief was: age < 65 years (p=0.029). We analysed 6 SNPs in the gene promoter region of IL-6 (-597 G/A, -572 G/C, -174 G/C) and IL-10 (-592 A/C, -819 C/T, -1082 A/G) as well as their relation with pain severity and analgesic consumption. Patients who are IL-10 -1082 A/G carriers are prone to respond better to radiotherapy than other patients (p<0.05). A borderline association was noted for patients who are IL-6 -597 A/A and G/G carriers - assumed to be at higher risk for severe pain prior to radiotherapy (p=0.07) while for patients who are IL-10 -1082 A/A carries: the median pain score decreased faster (p=0.08). Patients with genotypes IL-6 -597 A/A and IL-6 -174 C/C required a smaller dose of opioids (p=0.06). SNP of IL-6 and IL-10 cytokines can influence the analgesic response of radiotherapy. Patients with genotype IL-10 -1082 A/G respond better to radiotherapy.

Key words: Multiple myeloma, single gene polymorphism, pain relief.

Corresponding author: Milda Rudzianskiene, Oncology Institute of Lithuanian University of Health Sciences, Eiveniu 2, Kaunas, 50009, Lithuania, Tel. 00370 37 326954, Fax. 00370 37 326413, E-mail: mildalietuva@yahoo.com

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Published in „GENETIKA“ Vol. 46, No. 2(2014), pp. 471-484

Serbia

UDC 575.630
DOI: 10.2298/GENSR1402471P

Original scientific paper

STUDY OF RED CLOVER WILD POPULATIONS FROM THE TERRITORY

OF SERBIA FOR THE PURPOSE OF PRE-SELECTION

Mirjana PETROVIĆ^1*, Zora DAJIĆ – STEVANOVIĆ², Dejan SOKOLOVIĆ¹,

Jasmina RADOVIĆ¹, Jasmina MILENKOVIĆ¹, Jordan MARKOVIĆ¹

¹Institute for forage crops, Kruševac, Serbia

² Faculty of Agriculture, Universety of Belgrade, Belgrade, Serbia

Abstract

Petrović M., Z. Dajić – Stevanović, D. Sokolović, J. Radović, J. Milenković, J. Marković (2014): Study of red clover wild populations from the territory of serbia for the purpose of pre-selection. Genetika, Vol 46, No. 2, 471-484.

The aim of this paper was to evaluate the agronomic value of red clover (Trifolium pratense L.) wild populations. The analyzed material was gathered on the territory of Serbia and it was estimated which populations could be involved in the breeding program. 17 red clover populations were included in the research. Investigation was carried out at the experimental field of the Institute for forage crops, Kruševac, Serbia. The field trial was performed during three years (2008 - 2010) when data about morphological (green mass, dry mater, plant height, number of stems, number of lateral branches, number of internodes, length and width of a middle leaf lamina) and basic chemical parameters (quantity of crude proteins, crude fiber, ash, fat, NFE) were collected. In this paper, results of the research were processed by application of analysis of variance (ANOVA) and multivariation methods. Cluster analysis based on morphological traits resulted in formation of three clusters and the traits with the largest influence on grouping were: green mass, plant height, length and width of a leaf. Principal component method confirmed this separation and resulted in even more clear grouping of populations. Cluster analysis and PCA method were also applied to the traits of dry matter quality, when four clusters were formed, and the traits with the largest influence on grouping were percentages of protein, fat and fiber. Using both set of traits and several types of analyses contribute to grouping of populations which promise most and whose positive traits such as: high green mass yield, number of stems as well as the percentage of proteins can be combined for the purpose of getting synthetic varieties.

Key words: red clover, wild populations, morphological traits, quality of forage, cluster analysis.

Corresponding author: Mirjana Petrović, Institute for forage crops, 37251 Globoder, Kruševac, Serbia tel.: +38137 442 590, fax: +38137 441 295, e-mail: mirjana.petrovic@ikbks.com

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Published in „GENETIKA“ Vol. 46, No.2(2014), pp. 485 - 493

Serbia

UDC 575:630
DOI: 10.2298/GENSR1402485G

Original scientific paper

IN VITRO PLANT REGENERATION OF TWO CUCUMBER (Cucumis sativum L.) GENOTYPES: EFFECTS OF EXPLANT TYPES AND CULTURE MEDIUM

Stanislava GROZEVA and Nikolay VELKOV

Maritsa Vegetable Crops Research Institute – Plovdiv, Plovdiv, Bulgaria

Abstract

Grozeva S. and, N. Velkov (2014): In vitro plant regeneration of two cucumber (Cucumis sativum L.) genotypes: effects of explant types and culture medium-. Genetika vol 46, No2, 485-493.

The effect of different phytohormone concentrations on callusogenesis and organogenesis in two cucumber genotypes were studied. It was established that the rate of plant regeneration depends on genotype, explant type and culture medium. Hypocotyls were found to be more responsive than cotyledons in morphogenesis. In vitro planlet-regenerants have been obtained in hypocotyls explants on culture medium with 1.0 and 2.0 mgL^-1 BA for cultivar Gergana and in 1.0 and 3.0 mgL^-1 K – line 15B. Induction of regeneration in cotyledons were established only in cultivar Gergana on culture medium supplemented with 3.0 mgL^-1 BA and in combination of 0.5 mgL^-1IAA.

Key words: cucumber, callusogenesis, genotype, plant growth regulators, organogenesis

Corresponding author: Stanislava Grozeva, Department of Breeding, Variety Maintenance and Introduction, Maritsa Vegetable Crops Research Institute, Brezovsko shosse 32, 4003 Plovdiv, Bulgaria, Tel.: +35932952296, Fax: +35932960177, e-mail: stanislava_grozeva@abv.bg;

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Published in „GENETIKA“ Vol. 46, No. 2(2014), pp. 495- 504

Serbia

UDC 575:630

DOI: 10.2298/GENSR1402495B

Original scientific paper

SHOOT AND ROOT DRY WEIGHT IN DROUGHT EXPOSED

TOMATO POPULATIONS

Milka BRDAR-JOKANOVIĆ¹, Zdenka GIREK², Suzana PAVLOVIĆ²,

Milan UGRINOVIĆ², Jasmina ZDRAVKOVIĆ²

¹Institute of Field and Vegetable Crops, Novi Sad, Serbia

²Institute for Vegetable Crops, Smederevska Palanka, Serbia

Abstract

Brdar-Jokanović M., Z. Girek, S. Pavlović, M. Ugrinović, J. Zdravković (2014): Shoot and root dry weight in drought exposed tomato populations. Genetika, Vol 46, No. 2, 495-504.

This research was conducted with the aim to among forty-one tested tomato (Lycopersicon esculentum Mill) populations distinguish those tolerant to limited water supply. Tolerance assessments were performed by using sixteen drought stress selection indices calculated on the basis of tomato shoot and root dry weight yields determined at water stress and non-stress conditions. Populations were differentiated in groups using the method of cluster analysis. The pot experiment was set in controlled greenhouse conditions and comprised optimally irrigated control and drought treatment (35.0 and 20.9% volumetric soil water content, respectively), imposed at the phase of intensive vegetative growth. The experiment was conducted at the Institute for Vegetable Crops in Smederevska Palanka, Serbia. The analyzed tomatoes exhibited significant differences in terms of response to limited irrigation, which had more pronounced effect on shoot dry weight than on the roots (average decrease of 64.4 and 35.7%, respectively). Consequently, root fraction in the total dry weight increased at drought for 68.2% on average. Shoot and root dry weights were positively correlated at optimal irrigation but not in drought, implying genotypic differences in terms of root adjustments to stress conditions. As for the calculated selection indices, substantial variation was found among the populations enabling their ranking in terms of drought tolerance. Since ranking was not the same in all cases, clustering the populations was performed taking into account all sixteen selection indices. The results of this analysis indicate that populations designated with numbers 126, 124, 131, 125, 128, 105, 101, 138, 110, 132 and 109 in Institute for Vegetable Crops germplasm collection exhibit satisfactory level of drought tolerance at vegetative phase and therefore may be used as parents in breeding programs.

Key words: drought, stress selection indices, tomato, vegetative growth

Corresponding author: Milka Brdar-Jokanović, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia, Phone: 381 21 780 365, Fax: 381 21 780 198, E-mail: milka.brdar@nsseme.com, milka.brdarjokanovic@gmail.com

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Published in „GENETIKA“ Vol. 46, No. 2(2014), pp. 505- 514

Serbia

UDC 575:633
DOI: 10.2298/GENSR1402505B

Original scientific paper

GENETIC VARIABILITY AND ASSOCIATION PATTERN AMONG QUANTITATIVE NUTRITIONAL TRAITS IN SWISS CHARD (Beta vulgaris subsp. L. var. cicla) ACCESSIONS AND ITS IMPLICATION FOR BREEDING

M. Kadri BOZOKALFA,*^aBülent YAĞMUR^b Dursun EŞİYOK.^a Tansel KAYGISIZ AŞÇIOĞUL

^a Department of Horticulture, Faculty of Agriculture, Ege University, Bornova. İzmir, Turkey

^bDepartment of Soil Science, Faculty of Agriculture, Ege University, Bornova , İzmir, Turkey

Abstract

Bozokalfa Kadri M,. B. Yağmu, D. Eşiyok, T. Kaygisiz Aşçioğul (2014): Genetic variability and association pattern among quantitative nutritional traits in swiss chard (Beta vulgaris subsp. L. var. cicla) accessions and its implication for breeding.-Genetika, Vol 46, No. 2, 505-514.

In addition to improving agronomic traits, enriched cultivar such as nutritional elements and health promoting compounds are new demands for today's and the future's perspectives of crop breeding. In this respect, among leafy vegetables Swiss chard is a good source of nutritional elements and supplies large amounts of health promoting compounds. The existing knowledge of genetic variability for mineral composition both at the phenotype and genotype level, heritability of characters and also relationships among investigated minerals is fundamental for variety selection in Swiss chard. This also applies for the assurance of desirable agronomic traits with optimum mineral concentrations. This research analysis of variance indicated highly significant differences among Swiss chard accessions for all investigated mineral concentrations and the accessions display higher phenotype coefficient variation than genotype coefficient variation for all traits. The results revealed that phosphorus, magnesium, sodium, iron, copper, zinc, manganese, nitrate and nitrite exhibited high genetic advance accompanied with high heritability (>60%). The remaining mineral content demonstrated high heritability with moderate genetic advance. Genotype correlations were higher than the phenotype correlation for significant mineral concentrations. Genotype and phenotype correlations followed similar trends in all significant cases indicating the high heritable nature of the characters and the results showed that Swiss chard accessions should allow for the selection of individuals for enriched mineral concentration in edible parts of the plant.

Key words: Association analysis, mineral concentration, selection parameters, Swiss chard, variability

Corresponding author: M. Kadri Bozokalfa, Department of Horticulture, Faculty of Agriculture, Ege University, Bornova 35100 İzmir, Turkey. mehmet.kadri.bozokalfa@ege.edu.tr, Tel & Fax: +90 232 3881865

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Published in „GENETIKA“ Vol. 46, No. 2(2014), pp. 515-520

Serbia

UDC 575
DOI: 10.2298/GENSR1402515K

Original scientific paper

IDENTIFICATION OF CITRULLINAEMIA CARRIER AND DETECTION OF A NEW SILENT MUTATION AT 240bp POSITION IN ASS1 GENE OF NORMAL HOLSTEIN CATTLE

Rosaiah KOTIKALAPUDI¹,Rajesh Kumar PATEL²*, Richa Singh KUSHWAH¹

and Phani Sri Satish SUNKARA¹

¹ Sandor Lifesciences Pvt. Ltd., Banjara Hills, Hyderabad-500 034, India

² Sandor Animal Biogenics Pvt. Ltd., Banjara Hills, Hyderabad, India

Abstract

Kotikalapudi R., R. Kumar Patel, R. Singh Kushwah and P. Sri Satish Sunkara (2014): Identification of citrullinaemia carrier and detection of a new silent mutation at 240bp position in ass1 gene of normal holstein cattle. -.Genetika, Vol 46, No. 2,515 -520.

The autosomal recessive genetic disorders are regularly investigated especially in Indian Holstein and Holstein Crossbred bulls before they entered in semen collection so that a defective gene should not be transmitted to future generations. Bovine citrullinaemia first reported in Australia is a metabolic disorder as one of the enzymes, Argininosuccinate synthetase (ASS) involved in urea cycle is impaired in function. The mutation responsible for citrullinemia has been characterized as a single-base substitution at 256bp (C>T) in coding exon 3 of argininosuccinate synthetase 1 (ASS1) gene, which converts the CGA (arginine) at 86 codon to TGA (stop codon). A Holstein bull during routine molecular screening was found to be carrier for Citrullinaemia that was confirmed by sequencing. This is a fresh case of Citrullinaemia carrier in addition to three cases reported earlier in India. Partial sequencing of coding exon 3 of a normal Holstein revealed a new silent polymorphism at 240bp position that does not change amino acid (Sarine AGC>AGT) at 80 codon within exon 3 of ASS1 gene. The sequence of exon 3 of ASS1 gene in a normal Holstein exhibiting a new polymorphism was submitted to NCBI with accession No. KF933365. The presence of citrullinaemia carriers in Indian Holstein, though in very low frequency, emphasizing to continue the investigation of mutant gene in cattle population.

Key words: ASS1 gene, carrier, Citrullinaemia, Holstein, PCR, RFLP.silent mutation

Corresponding author: Rajesh Kumar Patel, Sandor Animal Biogenics Pvt. Ltd., Road No. 3, Banjara Hills, Hyderabad, India, rkpatel46@yahoo.com

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Published in „GENETIKA“ Vol. 46, No. 2(2014), pp. 521-528

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575:630
DOI: 10.2298/GENSR1402521B

Original scientific paper

GENOTYPE BY ENVIRONMENT INTERACTION AND STABILITY ANALYSIS

FOR RICE GENOTYPES UNDER BORO CONDITION

Lotan Kumar BOSE, Nitiprasad Namdeorao JAMBHULKAR and Kanailal PANDE

Central Rice Research Institute, Cuttack-753006, Orissa, India

Abstract

Bose Kumar L., N. Namdeorao Jambhulkar and K. Pande (2014): Genotype by Environment interaction and stability analysis for rice genotypes under Boro condition-. Genetika, Vol 46, No. 2, 521-528.

Genotype (G) × Environment (E) interaction of nine rice genotypes possessing cold tolerance at seedling stage tested over four environments was analyzed to identify stable high yielding genotypes suitable for boro environments. The genotypes were grown in a randomized complete block design with three replications. The genotype × environment (G×E) interaction was studied using different stability statistics viz. Additive Main effects and Multiplicative Interaction (AMMI), AMMI stability value (ASV), rank-sum (RS) and yield stability index (YSI). Combined analysis of variance shows that genotype, environment and G×E interaction are highly significant. This indicates possibility of selection of stable genotypes across the environments. The results of AMMI (additive main effect and multiplicative interaction) analysis indicated that the first two principal components (PC1–PC2) were highly significant (P<0.05). The partitioning of TSS (total sum of squares) exhibited that the genotype effect was a predominant source of variation followed by G×E interaction and environment. The genotype effect was nine times higher than that of the G×E interaction, suggesting the possible existence of different environment groups. The first two interaction principal component axes (IPCA) cumulatively explained 92 % of the total interaction effects. The study revealed that genotypes GEN6 and GEN4 were found to be stable based on all stability statistics. Grain yield (GY) is positively and significantly correlated with rank-sum (RS) and yield stability index (YSI). The above mentioned stability statistics could be useful for identification of stable high yielding genotypes and facilitates visual comparisons of high yielding genotype across the multi-environments.

Key words: boro rice, G×E interaction, stability

Corresponding author: Lotan Kumar Bose, Senior Scientist, Central Rice Research Institute, Cuttack-753006, Orissa, India, email: lotanrice@gmail.com

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Published in „GENETIKA“ Vol. 46, No. 2(2014), pp. 529-536

Serbia

UDC 575:630
DOI: 10.2298/GENSR1402529D

Original scientific paper

MOLECULAR CHARACTERIZATION AND GENETIC DIVERSITY ANALYSIS

β-GLUCAN CONTENT VARIABILITY IN GRAIN OF OAT (Avena sativa L.)

Nevena H. DJUKIĆ¹, Desimir S. KNEŽEVIĆ²

¹University of Kragujevac, Faculty of Science, Kragujevac, Serbia

²University of Priština, Faculty of Agriculture, Kosovska Mitrovica, Lesak, Kosovo and Metohia, Serbia

Abstract

Djukić N., D.Knežević (2014): β-glucan content variability in grain of oat (Avena sativa L.).- Genetika, vol., No.46, No 2, 529-536.

In grain of ten genetically divergent oat cultivars (Merkur, Minor Abed, Flaming-Kurz, Nuptiele, Prode, Pellerva, Emperor, Astor, Osmo, Simo) the variability β-glucan content were investigated. The different value of content of β-glucan was found. Among analyzed oat cultivars, the highest β-glucan contents had Pellerva (6.597%), while the least had Simo (2.971%). The contents of β-glucans were determined by ICC standard Method No 168. The value of β-glucans varied and indicated the differences and similarities between analysed cultivars. The degree of cultivar similarity was determined by dendrogram on which was discriminated two clusters of similar cultivars toward to contents of β-glucan . Within cluster 1, a small group of oats, are five cultivars with small distance (Merkur, Minor Abed, Flamings-Kurz, Nuptiele and Prode). The highest similarity in the range of 88 or the least distance in the range of 12. Within cluster 2 was four oat cultivars (Emperor, Astor, Osmo, Pellerva) in which the least differences was between Emperor and Astor with average distance in range 27. Cluster 1 and cluster 2 differed with an average distance of 63. The cultivar Simo expressed the greatest distance to all analysed oat cultivars grouped in two clusters.

Key words: β-glucan, content, divergence, oat, cultivars

Corresponding author: Nevena H. Djukić, University of Kragujevac, Faculty of Science, R. Domanovica 12, Kragujevac, Serbia,e-mail:nevena@kg.ac.rs

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Published in „GENETIKA“ Vol. 46, No. 2(2014), pp. 537-543

Serbia

UDC 575:630
DOI: 10.2298/GENSR1402537K

Original scientific paper

GENETIC DIVERSITY IN Brassica SPECIES AND Eruca sativa FOR YIELD ASSOCIATED PARAMETERS

Mahwish KANWAL¹, FARHATULLAH^1*, M. Ashiq RABBANI², Sidra IQBAL¹,

Laila FAYYAZ¹ and M. AFZAL¹

¹The University of Agriculture – Peshawar, Pakistan

²PGRI National Agriculture Research Center, Islamabad, Pakistan

Abstract

Kanwal Mahwish, Farhatullah, M. Ashiq Rabbani, S. Iqbal, L. Fayyaz and M. Afzal (2014): Genetic diversity in Brassica species and Eruca sativa for yield associated parameters- Genetika, vol., No.46, No.2, 537-543.

Brassica species are vulnerable to narrow genetic base due to the ignorance of their wild relatives which possess many superior characters. This study was aimed to explore the genetic diversity in five Brassica species from U triangle as well as in their wild relative Eruca sativa. For the complete insight of genetic diversity, four accessions, each from five species of genus Brassica along with one species of Eruca collected from different geographical locations (exotic and indigenous) were selected. Six yield associated parameters viz., primary branches plant^-1, plant height, main raceme length, silique length, silique width and silique main raceme^-1 were studied. Highly significant variations among all species were observed. Mean performance showed that wild relative E. sativa was superior for primary branches plant ^-1and plant height, which are the main yield associated traits. In case of Brassica species, B. campestris gave the lengthiest main racemes, B. nigra produced more silique main raceme^-1 and B. carinata produced the longest and widest silique.

Key words: Brassica,genetic divergence, E.sativa, wild relatives

Corresponding author: Farhatullah, The University of Agriculture – Peshawar, Pakistan, drfarhat@aup.edu.pk

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Published in „GENETIKA“ Vol. 46, No. 2(2014), pp. 545-559

Serbia

UDC 575:630
DOI: 10.2298/GENSR1402545J

Original scientific paper

MULTIVARIATE ANALYSIS OF QUANTITATIVE TRAITS CAN EFFECTIVELY CLASSIFY RAPESEED GERMPLASM

Mirjana JANKULOVSKA^1*, Sonja IVANOVSKA¹, Ana MARJANOVIC-JEROMELA², Snjezana BOLARIC³, Ljupcho JANKULOSKI¹, Zoran DIMOV ¹, Dane BOSEV¹,

Biljana KUZMANOVSKA¹

¹Faculty ofAgricultural Sciences and Food, Ss. Cyril and Methodius University,

Skopje, Macedonia

²Institute of Field and Vegetable Crops, Novi Sad, Serbia

³Faculty of Agriculture, University of Zagreb, Zagreb, Croatia

Abstract

Jankulovska M., S. Ivanovska, A. Marjanovic-Jeromela, S. Bolaric, LJ. Jankuloski, Z. Dimov, D. Bosev, B. Kuzmanovska (2014): Multivariate analysis of quantitative traits can effectively classify rapeseed germplasm. Genetika, Vol 46, No. 2, 545-559.

In this study, the use of different multivariate approaches to classify rapeseed genotypes based on quantitative traits has been presented. Tree regression analysis, PCA analysis and two-way cluster analysis were applied in order todescribe and understand the extent of genetic variability in spring rapeseed genotype by trait data.

The traits which highly influenced seed and oil yield in rapeseed were successfully identified by the tree regression analysis. Principal predictor for both response variables was number of pods per plant (NP). NP and 1000 seed weight could help in the selection of high yielding genotypes. High values for both traits and oil content could lead to high oil yielding genotypes. These traits may serve as indirect selection criteria and can lead to improvement of seed and oil yield in rapeseed. Quantitative traits that explained most of the variability in the studied germplasm were classified using principal component analysis. In this data set, five PCs were identified, out of which the first three PCs explained 63% of the total variance. It helped in facilitating the choice of variables based on which the genotypes’ clustering could be performed. The two-way cluster analysissimultaneously clustered genotypes and quantitative traits. The final number of clusters was determined using bootstrapping technique. This approach provided clear overview on the variability of the analyzed genotypes. The genotypes that have similar performance regarding the traits included in this study can be easily detected on the heatmap. Genotypes grouped in the clusters 1 and 8 had high values for seed and oil yield, and relatively short vegetative growth duration period and those in cluster 9, combined moderate to low values for vegetative growth duration and moderate to high seed and oil yield. These genotypes should be further exploited and implemented in the rapeseed breeding program. The combined application of these multivariate methods can assist in deciding how, and based on which traits to select the genotypes, especially in early generations, at the beginning of a breeding program.

Key words: multivariate techniques, principal component analysis, rapeseed tree regression analysis, two-way cluster analysis,

Corresponding author: Mirjana Jankulovska, Department of Genetics and Plant Breeding, Faculty of Agricultural Sciences and Food, Ss Cyril and Methodius University in Skopje, blvd. Aleksandar Makedonski bb, 1000 Skopje, Republic of Macedonia, tel: ++389 2 3115 277, fax: ++389 2 3134 210, e-mail: mjankulovska@zf.ukim.edu.mk

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Published in „GENETIKA“ Vol. 46, No. 2(2014), pp. 561-568

Serbia

UDC 575
DOI: 10.2298/GENSR1402561C

Original scientific paper

SECULAR CHANGES IN CEPHALIC INDEX-A STUDY

OF SERBIAN SCHOOL CHILDREN

Milena CVETKOVIĆ¹, Stevo NAJMAN², Miloš NIKOLIĆ²

¹Elementary school Sreten Mladenović Mika Niš, Serbia

²Faculty of Medicine, University of Niš, Serbia

Abstract

Cvetković M., S. Najman, M. Nikolić (2014): Secular changes in cephalic index-a study of Serbian school children.- Genetika, Vol 46, No. 2, 561-568.

Craniofacial anthropometry is used to determine similarities and differences within a single population or between populations, as well as to determine the secular trends. The aim of the study is to determine differences in the cephalic index of school children from the city of Niš (southeastern Serbia) in two periods-in 1983 and 2010. Subjects were aged 7-15. The first group of subjects (from 1983) included 968 children of both genders, while the second group of subjects (from 2010) included 1037 children, also of both genders. The test was performed according to the instructions of the International Biology Program (IBP). The head length and head breadth were measured, and based on these parameters the cephalic index was determined. The results show that during the time debrachycephalization appeared, because brachycephalic was dominant with the children from 1983, while mesocephalic was dominant with the second group (2010). Genderual dimorphism exists in both groups of subjects.

Key words: cephalic index, children, debrachycephalization, head dimensions, secular changes, Serbia

Corresponding author: Cvetković Milena, Elementary School Sreten Mladenović Mika, Šabačka 20, Niš, Serbia; phone: +381184232869, fax +381184535300 e-mail: tasan@open.telekom.rs

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Published in „GENETIKA“ Vol. 46, No. 2(2014), pp. 569-578

Serbia

UDC 575
DOI: 10.2298/GENSR1402569M

Original scientific paper

PATTERN OF QUANTITATIVE INHERITANCE OF YIELD AND COMPONENT TRAITS IN OPIUM POPPY (Papaver somniferum L.)

Krishna Nand MAURYA¹, *Sudhir SHUKLA¹ and Geeta ASTHANA²

¹National Botanical Research Institute, Rana Pratap Marg, Lucknow-226001, India

²Lucknow University, Lucknow

Abstract

Maurya Krishna N., S. Shukla and G. Asthana (2014): Pattern of quantitative inheritance of yield and component traits in opium poppy (Papaver somniferum L.).-Genetika, Vol 46, No. 2, 569--578.

Generation mean analysis of cross NB-5x58/1 and its reciprocal cross was carried out to understand the nature of gene action in opium poppy. The significance of A, B, C and D scaling tests indicated presence of non-allelic interaction in the inheritance of traits except capsule size and husk yield/plant for reciprocal cross. Additive as well as dominance components of gene action were found in both the crosses. Most of the traits had greater non fixable dominance ‘h’ and dominance x dominance effects ‘l’ than fixable additive (d) and additive x additive effects (i) except leaves/plant, branches/plant, capsules/plant, stem diameter, capsule weight/plant, husk yield/plant, opium yield/plant, codeine and narcotine content which showed greater importance of additive (d) and additive x additive effects (i) effects. Inter-mating of the best parents, diallel selective mating or biparental mating in early segregating generations followed by recurrent selections were suggested for genetic improvement of opium poppy.

Key words: alkaloid, epistasis, gene action, Joint scaling test, Papaver somniferum L.

Corresponding author: Sudhir Shukla, National Botanical Research Institute, Rana Pratap Marg, Lucknow-226001, India

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Published in „GENETIKA“ Vol. 46, No. 2(2014), pp579-590

Serbia

UDC 575:633.15
DOI: 10.2298/GENSR1402579K

Original scientific paper

GENETIC AND BIOCHEMICAL CHARACTERISATION OF PARENTAL INBRED LINES IN MARKER ASSISTED SELECTION FOR QUALITY

PROTEIN MAIZE

Marija KOSTADINOVIC, Dragana IGNJATOVIC-MICIC, Goran STANKOVIC, Jelena VANCETOVIC, Danijela RISTIC, Snezana MLADENOVIC DRINIC

Maize Research Institute Zemun Polje, Belgrade, Serbia

Abstract

Kostadinovic M., D. Ignjatovic-Micic, G. Stankovic, J. Vancetovic, D. Ristic, S. Mladenovic Drinic (2014): Genetic and biochemical characterisation of parental inbred lines in marker assisted selection for quality protein maize-. Genetika, Vol 46, No. 2, 579-590.

Maize protein is deficient in two essential amino acids, lysine and tryptophan. Naturally occurring opaque2 (o2) mutation which increases the levels of tryptophan and lysine in the grain also confers an undesirable phenotype leading to low yields and more susceptible, soft and chalky kernels. Quality Protein Maize (QPM) is agronomically acceptable and nutritionally improved opaque2 maize. Marker assisted selection increases reliability and efficiency, reduces time and costs taken to obtain QPM. The results presented in this paper are a part of the Maize Research Insitute project on conversion of normal maize lines to QPM lines adapted for growing in temperate regions trough marker assisted backcross (MAB). Genetic and biochemical variability was analyzed between normal (ZPL 5) and QPM (CML 144) parental inbred lines and the efficiency of the three opaque2 specific SSR markers was examined. Markers phi057 and umc1066 can discriminate homozygous and heterozygous backcross progeny, thus can be used as foreground selection markers for the opaque2 gene. The genetic similarity between analyzed lines was 0.05, which confirmed good selection of parental lines for the creation of hybrids. Tryptophan content was 0.093 and QI was 0.85 in CML 144, approximately twice as high compared to 0.054 and 0.48 in ZPL 5. Both tryptophan and QI values for CML 144 were above the QPM treshold, i.e. tryptophan for 24% and QI for 0.625%, making it a suitable donor line of o2. These results represent the framework for marker assisted introgression of the quality protein trait into local maize genotype.

Key words: maize, marker assisted selection, opaque2, Quality Protein Maize, SSR

Corresponding author: Marija Kostadinovic, Maize Research Institute Zemun Polje, Belgrade, Serbia, Phone: 011 3756704, Fax: 011 3756707, e-mail: kmarija@mrizp.rs

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Published in „GENETIKA“ Vol. 46, No. 2(2014), pp. 591-599

UDC 575
DOI: 10.2298/GENSR1402591F

Original scientific paper

ASSOCIATION OF POLYMORPHISMS IN THE PROMOTER REGION

OF TURKEY PROLACTIN WITH EGG PERFORMANCE

Mehrangiz FATHI¹, Ghorban ELYASI ZARRINGHOBAIE²

¹Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran.

²Department of Agriculture Research Center of East Azerbaijan ,Tabriz, Iran

Abstract

Fathi ,M., G. Elyasi Zarringhobaie (2014): Association of polymorphisms in the promoter region of turkey prolactin with egg performance. Genetika, Vol 46, No. 2, 591 -599.

The induction and regulation of broodiness is of the most important role of prolactin in avian species. In this study, the association between prolactin promoter region alleles and reproductive traits in Fars native turkey was investigated . These traits consisted of mean egg weight (MEW), number of egg (EN) and egg mass, during the first laying period .In total, 115 laying turkeys, randomly selected from the flock of the Breeding Center for Fars Native turkey, and DNA was purificated from blood samples, 231 bp of prolactin promoter region was amplified and Genotype of Samples was determinate by PCR-SSCP technique were genotyped . Two alleles D and I were identified. Based on the results obtained, the frequency of D and I alleles were 0.67 and 0.33, respectively. Frequencies of DD, II and ID genotypes were 0.385, 0.044 and 0.571, respectively. The association analysis between the polymorphism PRL gene promoter region and egg performance was carried out. Significant relationship was found between genotypes with egg production (P<0.01). Individuals with II genotype produced higher egg production than DD and ID genotype. The results of current study showed that using information of genes related to egg production could be used to improve the performance of native turkey of East Azerbaijan province.

Key words: egg performance, polymorphism, prolactin promoter, turkey

Corresponding author: M. Fathi, Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran. E-mail: mehrangizfathi@gmail.com, Phone: + 9149032101.

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Published in „GENETIKA“ Vol. 46, No. 2(2014), pp. 601-610

Serbia

UDC 575
DOI: 10.2298/GENSR1402601D

Original scientific paper

INCIDENCE AND CLINICAL RELEVANCE OF T(11;19) TRANSLOCATION

IN SALIVARY GLAND MUCOEPIDERMOID CARCINOMA

Ivana ILIĆ DIMITRIJEVIĆ ¹_,Thomas LÖNING ²_,Marcus FALK ²,Vitomir S. KONSTANTINOVIĆ¹_,Miroslav VUKADINOVIĆ¹, Zvezdana TEPAVČEVIĆ ³_,Saša TABAKOVIĆ⁴_,Nataša PEJČIĆ ¹, Biljana MILIČIĆ⁵, Jelena MILAŠIN ⁵

1. University of Belgrade, School of Dental Medicine, Clinic of Maxillofacial Surgery, Belgrade, Serbia

2. Institute of Haematopathology Hamburg, Hamburg, Germany

3. University of Belgrade, School of Dental Medicine, Department of Pathology, Belgrade, Serbia

4. University of Priština, Medical Faculty, Department of Dental Medicine, Kosovska Mitrovica, Priština, Serbia

5. University of Belgrade, School of Dental Medicine, Department of Human Genetics, Belgrade, Serbia

Abstract

Dimitrijević Ilić I._,T. Löning_,M. Falk,V. S. Konstantinović_,M. Vukadinović, Z. Tepavčević_,S. Tabaković_,N.Pejčić B. Miličić, J. Milašin (2014): Incidence and clinical relevance of t(11;19) translocation in salivary gland mucoepidermoid carcinoma-Genetika, Vol 46, No.2, 601 -610.

Mucoepidermoid carcinoma (MEC) harbors a recurring t(11;19) translocation with an associated novel fusion oncogene-MECT1-MAML2. The CRTC1-MAML2oncogene disrupts normal cell-cycle and differentiation, contributing to tumor development. The objectives of this study were to establish the incidence of CRTC1-MAML2 fusion in Serbian patients and estimate its relevance as a genetic marker of MEC behavior. In this retrospective study, 20 cases of MEC of salivary glands were tested for the presence of CRTC1-MAML2 fusion using reverse transcriptase-polymerase chain reaction. Clinicopathological parameters and survival data were examined in relation to fusion status. The CRTC1-MAML2 fusion was detected in 40% of MECs and its presence was associated exclusively with low-intermediate grade tumor histology (P = 0.02) and favorable clinical outcome, with 100 % overall survival rate (P=0.046). The study has shown that the presence of the CRTC1-MAML2 fusion can serve as an additional diagnostic and prognostic marker for mucoepidermoid carcinomas.

Key words: Salivary gland, Mucoepidermoid carcinoma, Chromosomal translocation (11,19), MECT1-MAML2, RT-PCR diagnostic

Corresponding author: Jelena Milašin,Department of Human Genetics,School of Dental Medicine, University of Belgrade,Dr Subotića 4, 11000 Belgrade, Serbia,Email: jelena.milasin@stomf.bg.ac.rs,Tel:+381 63 7596 550,Fax: +381 11 685 361

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Published in „GENETIKA“ Vol. 46, No. 2(2014), pp. 611-620

Serbia

UDC 575
DOI: 10.2298/GENSR1402611G

Original scientific paper

MUTATION SCREENING OF FAMILIAL MEDITERRANEAN FEVER IN THE AZERI TURKISH POPULATION: GENOTYPE–PHENOTYPE CORRELATION AND THE CLINICAL PROFILE VARIABILITY

Jalal GHARESOURAN¹, Maryam REZAZADEH^1,2, Morteza GHOJAZADEH³,

Mojtaba MOHADDES ARDABILI¹

¹Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

²Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran

³ Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences ,Tabriz, Iran

Abstract

Gharesouran J., M. Rezazadeh, M. Ghojazadeh, M. Mohaddes Ardabili (2014): Mutation screening of familial mediterranean fever in the azeri turkish population: genotype–phenotype correlation and the clinical profile variability. Genetika, Vol 46, No. 2, 611-620.

Familial Mediterranean fever is known as a most frequent hereditary autoin-Xammatory among the autoinflammatory syndromes characterised by fever, arthritis and serosal inflammation. Clinically, the foremost severe symptom of the disease is amyloidosis, which may cause to renal failure. MEFV renal failure consists of ten exons and conservative mutations clustered in exon ten (M694V, V726A, M680I, M694I) and exon two (E148Q) are considered more common mutations within this coding region and that they are detected with a distinct frequency changes in line with ethnicity. The aim of this study was to research the spectrum of mutations in Azeri Turkish population.

We evaluated the molecular test results of 82 patients and their parents from eighty families identified as having FMF clinical symptoms referred to Molecular Genetics Laboratory of the Department of Medical Genetics. Patients were referred by their physicians for MEFV mutation detection. The most frequent mutations were M694V respectively followed by M680I (G/C), V726A, M694I and E148Q mutations. A phenotypic variability was also ascertained between patients with different mutations and it must be considered within the daily management of FMF patients.

Key words: Familial Mediterranean fever, 16p13.3, Azeri Turkish

Corresponding author: Jalal Gharesouran,Member of Academic Board,Faculty of medicine,Tabriz University of Medical Sciences,Tabriz-Iran,Tel/Fax: ++98-411-3371587,e-mail: Jalal.Gharesouran@gmail.com

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Published in „GENETIKA“ Vol. 46, No. 2(2014), pp. 621-629

Serbia

UDC 575
DOI: 10.2298/GENSR1402621N

Original scientific paper

PHENOTYPIC PRESENTATION OF THROMBOPHILIA IN DOUBLE HETEROZYGOTE FOR FACTOR V LEIDEN AND PROTHROMBIN 20210 G>A MUTATIONS – CASE REPORT

Ljudmila NAGORNI-OBRADOVIĆ^1,2, Nela MAKSIMOVIĆ¹, Predrag MILJIĆ ^1,3, Dragana CVETKOVIĆ⁴, Ruža STEVIĆ^1,5, Dragica PEŠUT^1,2Ž

¹ Faculty of Medicine, University of Belgrade, Serbia; ². Clinic for Pulmology, Clinical Centre of Serbia, Belgrade, Serbia;³.Clinic for Hematology, Clinical Centre of Serbia,

Belgrade, Serbia; ^4. Faculty of Biology, University of Belgrade, Serbia;⁵.Clinic for Radiology, Clinical Centre of Serbia, Belgrade, Serbia

Abstract

Nagorni-Obradović Lj., N. Maksimović, P. Miljić, D. Cvetković, R. Stević, D. Pešut (2014): Phenotypic presentation of thrombophilia in double heterozygote for factor v leiden and prothrombin 20210 g>a mutations – case report. Genetika, Vol 46, No. 2, 621 -629.

Physicians usually do not suspect pulmonary thromboembolism in younger patients except in those who have thrombophilia. In those latter patients some special conditions such as trauma or surgery may provoke the disease. In some adult persons, thrombophilia may still remain unrecognized, until appearance of additional conditions influence development of thrombosis. A 55-year-old Caucasian female, non- smoker, experienced sudden chest pain and hemoptysis without chest trauma. History taking revealed type 2 diabetes mellitus and hypothyroidism. She was overweight with body mass index 29.0. The review of the family history revealed that her father and mother died of brain infarction, while her 22-year-old son and 24-year-old daughter were healthy. Due to suspicion for thrombosis, multi-slice computerized tomography thorax scan was done and pulmonary embolism was diagnosed. Although without clear risk factor for thrombosis in our patient, we performed laboratory investigation for congenital thrombophilia. Genetic analysis showed double heterozygous for factor V Leiden and prothrombin 20210 G>A mutations. Congenital thrombophilia was risk factor for thrombosis in our patient but haemostatic imbalance was not previously clinically recognized. She had two pregnancies without complications. Appearance of other associative factors such as endocrine disorders - hypothyroidism and metabolic syndrome with diabetes type 2, and overweigh were additional potential triggers for clinical manifestation of pulmonary thromboembolism in her adult age. Her children underwent genetic analysis, too. The son was also double heterozygous for factor V Leiden and prothrombin 20210 G>A mutations, while daughter was heterozygous for factor V Leiden, and none had clinical signs of thrombosis

Key words: pulmonary thromboembolism, thrombophilia, risk factors, genetic.

Corresponding author: Ljudmila Nagorni-Obradović, MD, PhD; Faculty of Medicine, University of Belgrade, Serbia ; Clinic for Pulmology, Clinical Center of Serbia,11000 Belgrade, Koste Todorovica 26,Republic of Serbia; Tel. +381 11 366 3956; Fax. +381 11 2681 591; E-mail: ljudmila.nagorni@kcs.ac.rs

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Published in „GENETIKA“ Vol. 46, No.2(2014), pp. 631-647

Serbia

UDC 575
DOI: 10.2298/GENSR1402621G

Original scientific paper

GENETIC AND NON-GENETIC FACTORS RESPONSIBLE FOR MITOCHONDRIAL FAILURE AND ALZHEIMER’S DISEASE

Kuo GAO, Meiying NIU, Xing ZHAI, Youliang HUANG, Xin TIAN, Tiangang LI

Beijing University of Chinese Medicine, Chao Yang District, Beijing, China

Abstract

Gao K., M. NIu, X. Zhai,Y. Huang, X. Tian, T. Li (2014): Genetic and non-genetic factors responsible for mitochondrial failure and alzheimers disease- Genetika, Vol 46, No. 2, 631 -647

The objective of this review article is to explain the factors responsible for damaged mitochondria and its association with Alzheimer’s disease. Alzheimer’s disease (AD) is fairly produced by dysfunctional mitochondria that are alternatively caused by excessive reactive oxygen species and mitochondrial dynamic imbalance. In the pathogenesis of AD, there is important role of many factors including amyloid-beta peptide (A𝛽), tau-proteins, and mutations in presenilin-1. Additionally, mitochondrial-targeted antioxidants have also been explained because of their significance to mitochondrial alterations in AD. Moreover, alteration in mitochondrial dynamics is responsible for the generation of segregated, damaged mitochondria that are, later on, destroyed through mitochondrial autophagy in AD. Finally, various novel models used for studying Alzheimer’s disease, have been discussed.

Key words: amyloid-beta peptide, brain, mitochondrial dysfunction, neurofibrillary tangles, senile plaques, tau-protein

Corresponding author: Kuo Gao, Beijing University of Chinese Medicine, Chao Yang District, Beijing, China

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