GENETIKA, Vol. 54, No.1 (2022)
Željko LAKIĆ, Vera POPOVIĆ, Milivoje ĆOSIĆ, Marina ANTIĆ
GENOTYPES VARIATION OF Medicago sativa (L.) SEED YIELD COMPONENTS IN ACID SOIL UNDER CONDITIONS OF CROSS – FERTILIZATION [Abstract] [Full text]
Mohammad Hossein ROMENA, Abdollah NAJAPHY, Mohsen SAEIDI, Mahmood KHORAMIVAFA
IDENTIFICATION OF SUPERIOR WHEAT GENOTYPES USING MULTIPLE-TRAIT SELECTION METHODS BASED ON AGRONOMIC CHARACTERS AND GRAIN PROTEIN CONTENT UNDER RAIN-FED CONDITIONS [Abstract] [Full text]
Hari KESH and Khushi RAM
PERFORMANCE OF BASMATI RICE (Oryza sativa L.)
GENOTYPES UNDER DIFFERENT CROP ESTABLISHMENT METHODS [Abstract] [Full
text]
Mathew Adekunle ADELEKE, Kazeem Olajide BELLO, Ilesanmi Oyindamola AKINYEMI, Oluwakemi Titilayo IREKHORE, Babatunde Moses ILOR3, Oluwatosin Adetutu ADEYEYE, Samuel Ayodele FAMAKINDE
MORPHOLOGICAL VARIATIONS IN QUALITATIVE AND QUANTITATIVE TRAITS OF FULANI ECOTYPE CHICKENS IN NIGERIA[Abstract] [Full text]
Maryam NIKKHAH, Sedigheh ARBABIAN, Ahmad MAJD, Fariba SHARIFNIA
GENETIC DIVERSITY OF Cordia myxa L. ASSESSED BY ISSR MARKERS [Abstract] [Full text]
Kerem MERTOĞLU
INVESTIGATION OF GENETIC PARAMETERS AND
PHYTOCHEMICAL CHARACTERISTICS IN PLUM UNDER ALTITUDE CHANGE [Abstract] [Full
text]
Umakanta SARKER, Md. Golam AZAM, Md. Zahirul Alam TALUKDER
GENETIC VARIATION IN MINERAL PROFILES, YIELD CONTRIBUTING AGRONOMIC TRAITS, AND FOLIAGE YIELD OF STEM AMARANTH [Abstract] [Full text]
Aydın UZUN, Hasan PINAR, Mehmet YAMAN, Merve A. YIGIT, Yaşar CAKIROGLU, Arif KARAKAYA, Mehmet UYSAL, Gökhan OZTURK, Kadir Uğurtan YILMAZ, Kahraman GURCAN, Sezai ERCISLI
IDENTIFICATION OF GENETIC DIVERSITY IN WILD PEAR (Pyrus Elaeagrifolia Pall.) GENOTYPES COLLECTED FROM DIFFERENT REGIONS OF TURKEY WITH SSR MARKER SYSTEM [Abstract] [Full text]
Yan ZHOU and Zhongbiao ZHENG
GENETIC DIVERSITY AND INTER-RELATIONSHIP AMONG Stellaria
L. (CARYOPHYLLACEAE) SPECIES BY ISSR MARKERS [Abstract] [Full
text]
Salih KESKIN, Yaşar AKCA, Sezai ERCISLI
GENETIC DIVERSITY OF WHITE MULBERRY (Morus alba
L.) ACCESSIONS SELECTED FROM NORTH
EASTERN TURKEY [Abstract] [Full
text]
Gulden HASPOLAT, Burak KUNTER, Yaprak KANTOGLU
DETERMINATION OF MUTAGENIC-SENSITIVITY AND INDUCED
VARIABILITY IN THE MUTANT POPULATIONS OF ‘BACARDI’ CHRYSANTHEMUM CULTIVAR [Abstract] [Full text]
Vikas GUPTA, Pradeep KUMAR, Gyanendra SINGH, Charan SINGH, Amit Kumar SHARMA, BS TYAGI, GP SINGH
MULTI-ENVIRONMENT CHARACTERIZATION OF BREAD WHEAT GENOTYPES FOR WATERLOGGING TOLERANCE [Abstract] [Full text]
Juan YIN
EVALUATION OF GENETIC VARIABILITY Rindera USING RAPD MARKERS [Abstract] [Full text]
Özge Doğanay ERBAŞ KÖSE, Zeki MUT, Hasan AKAY
GENETIC DIVERSITY FOR MINERAL ELEMENT IN SEEDS OF TURKISH OAT LANDRACES [Abstract] [Full text]
Seyed Ardalan AZIZI, Farah FARAHANI, Masoud SHEIDAI
SOMACLONAL VARIATION IN POMEGRANATE (Punica granatum L.): ISSR AND CYTOLOGICAL EVIDENCES [Abstract] [Full text]
Bayram Murat ASMA, Fırat Ege KARAAT, Çiğdem ÇUHACI
EVALUATION OF NEW TURKISH APRICOT GENETIC
RESOURCES FROM THE IRANO-CAUCASIAN ECO-GEOGRAPHICAL GROUP [Abstract] [Full
text]
Karamvir KAUR, Tarsem Singh DHILLON, Rajinder SINGH
GGE BIPLOT ANALYSIS FOR SEED PRODUCTION POTENTIAL OF EUROPEAN CARROT (Daucus carota L.) GENOTYPES IN PLAINS AND SUBMOUNTENOUS ZONES OF PUNJAB [Abstract] [Full text]
Fikri BAYINDIR, Yalçın COŞKUN
THE EFFECTS OF IRRIGATION WATER SALINITY ON THE SEED GERMINATION AND SEEDLING GROWTH OF RICE [Abstract] [Full text]
Gaurav KHOSLA, B S GILL, Asmita SIRARI, Pritpal SINGH
ASSESSMENT OF EFFICIENCY OF BREEDING METHODS USING MOLECULAR MARKERS IN SOYBEAN [Abstract] [Full text]
Song TING and Yu YIBING
POPULATION DIFFERENTIATION AND GENE FLOW OF Glaucium flavum (Papaveraceae) [Abstract] [Full text]
Dragan GRČAK, Snežana GOŠIĆ DONDO, Milosav GRČAK, Danijela RISTIĆ, Danijela KONDIĆ, Djuradj HAJDER, Željko POPOVIĆ, Desimir KNEŽEVIĆ
INFLUENCE OF MAIZE HYBRIDS AND APPLAYED INSECTICIDES ON Ostrinia nubilalis HBN. ATTACK [Abstract] [Full text]
A.J. SANDA, M.N. BEMJI, M. WHETO, A.O. OSO, M.O. SANDA, O. OLOWOFESO
GENETIC AND PHENOTYPIC CORRELATION COEFFICIENTS OF QUANTITATIVE TRAITS IN TWO CHICKEN GENOTYPES [Abstract] [Full text]
Ramazan SABIRLI, Aylin KÖSELER, Ergun METE, Ibrahim TÜRKÇÜER
ASSOCİATİON OF IL-1Β (-511 C / T) AND IL-1Β (-31 T / C) GENE POLYMORPHİSM WİTH ENDOPLASMİC RETİCULUM STRESS MARKER LEVELS İN ACUTE DECOMPENSATED HEART FAİLURE WİTH LOW EJECTİON FRACTİON [Abstract] [Full text]
Agit ÇETINKAYA, Ömür BAYSAL, Ragıp Soner SILME, M. Kamran AZIM, Faizan SALEEM
GENOMIC CHARACTERIZATION AND PHYTOSTIMULATIVE EFFECT OF A NOVEL SERRATIA SPECIES [Abstract] [Full text] [Supplement]
Yanxia SUN, Hanxiu JIANG, Fang ZENG, Xin PAN, Xiaoxia WU, Yue QI, Xiaoyong WU
SPECIES IDENTIFICATION AND GENETIC DIVERSITY OF Alcea (Malvaceae) USING SCOT MOLECULAR MARKERS: MEDICINAL PLANT [Abstract] [Full text]
Aref FATEHI, Esmail BABARASHI, Shahram MEHRI, Mehdi SALAJEGHEH
EVALUATION OF GRAIN YIELD OF WHEAT GENOTYPES USING STRESS TOLERANCE INDICES [Abstract] [Full text]
Marija BRANKOVIĆ, Nataša DRAGAŠEVIĆ, Valerija DOBRIČIĆ, Aleš MAVER, Gaber BERGANT, Igor PETROVIĆ, Stojan PERIĆ, Ana MARJANOVIĆ, Milena JANKOVIĆ, Jasna JANČIĆ, Ivana NOVAKOVIĆ, Borut PETERLIN, Marina SVETEL, Vladimir KOSTIĆ
CLINICAL EXOME SEQUENCING IN SERBIAN PATIENTS WITH MOVEMENT DISORDERS - SINGLE CENTRE EXPERIENCE [Abstract] [Full text]
Muhammad Usman Faryad KHAN, Asif NADEEM, Maryam JAVED, Wasim SHEHZAD, Muhammad Asad ALI
MOLECULAR CHARACTERIZATION OF METHICILLIN-RESISTANT Staphylococcus aureus (MRSA) ISOLATED FROM BOVINE WOUNDS [Abstract] [Full text]
Jelena STOJČEVIĆ MALETIĆ, Iva BARJAKTAROVIĆ, Vanja RADENKOVIĆ, Velibor ČABARKAPA
Seda Orenay BOYACIOGLU, Metin CALISKAN, Guzide DOGAN
RELATIONSHIP BETWEEN PEDIATRIC CELIAC DISEASE AND CHROMATIN REMODELING GENE EXPRESSIONS[Abstract] [Full text]
Bojana DOBRIC, Danijela
RADIVOJEVIC, Jovana JECMENICA, Pavlos FANIS,
Vassos NEOCLEOUS, Leonidas A PHYLACTOU, Marina DJURISIC
PREVALENCE OF VARIANTS IN DFNB1 LOCUS IN SERBIAN
PATIENTS WITH AUTOSOMAL RECESSIVE NON-SYNDROMIC
HEARING LOSS [Abstract] [Full text]
Svetlana JOVIČIĆ PAVLOVIĆ, Sanja SIMIĆ OGRIZOVIĆ, Zoran BUKUMIRIĆ, Milena ERIĆ, Natalija PAVLOVIĆ, Boba Kotlica, Ivana NOVAKOVIĆ
IMPACT OF THE FETUIN GENE POLYMORPHISMS IN CORONARY ARTERY CALCIFICATION AND MORTALITY OF PATIENTS WITH CHRONIC KIDNEY DISEASE AND RENAL TRANSPLANT [Abstract] [Full text]
Antonela MARKULJ KULUNDŽIĆ, Aleksandra SUDARIĆ, Maja MATOŠA KOČAR, Anto MIJIĆ, Ivica LIOVIĆ, Marija VILJEVAC VULETIĆ, Ivana VARGA, Vera CESAR, Hrvoje LEPEDUŠ
SUNFLOWER AGRONOMIC TRAITS IN FIELD IRRIGATION CONDITIONS [Abstract] [Full text]
Katarina JOVANOVIC, Jelena CARKIC, Nadja NIKOLIC, Nada NOVAKOVIC, Milos HADZI-MIHAILOVIC, Emina COLAK, Sasa CAKIC, Jelena MILASIN
DETECTION AND QUANTIFICATION OF PATHOGENIC MICROORGANISMS IN PATIENTS WITH CHRONIC PERIODONTITIS BEFORE AND AFTER TREATMENT WITH CHLORHEXIDINE DIGLUCONATE [Abstract] [Full text]
Deepa BENIWAL, R.K. DHALL, Saurabh YADAV, Priti SHARMA
AN OVERVIEW OF RUST (Uromyces viciae-fabae) AND POWDERY MILDEW (Erysiphe polygoni DC) OF PEA (Pisum sativum L.) [Abstract] [Full text]
Editor
CORRIGENDUM [Abstract] [Full text]
Published in „GENETIKA“ Vol. 54, No.1(2022), pp. 1-14
© 2022 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
UDC 575.633
https://doi.org/10.2298/GENSR2201001L
Original
scientific article
GENOTYPES VARIATION OF Medicago sativa (L.) SEED YIELD COMPONENTS
IN ACID SOIL UNDER CONDITIONS OF CROSS – FERTILIZATION
Željko LAKIĆ1,2, Vera POPOVIĆ3, Milivoje ĆOSIĆ4,5, Marina ANTIĆ6
1PI Agricultural Institute of Republic of Srpska, Banja Luka, Bosnia & Hercegovina
2University of Banja Luka, Faculty of Agriculture, Bosnia & Hercegovina
3Institute of Field and Vegetable Crops, Novi Sad, Republic of Serbia
4University of Bijeljina, Faculty of Agriculture, Bijeljina, Bosnia and Hercegovina
5Institute of Forestry, Belgrade, Republic of Serbia
6University of Banja Luka, Institute of Genetic Resources, Bosnia & Hercegovina
Abstract
Lakić Ž., V. Popović, M. Ćosić, M. Antić (2022). Genotypes variation of Medicago sativa (L.) seed yield components in acid soil under conditions of cross – fertilization - Genetika, Vol 54, No.1, 1 - 14.
Alfalfa (Medicago sativa L.) is the most important forage plant species in Bosnia and Herzegovina. In this study, conducted in Banja Luka, the investigated ten alfalfa genotypes originated from different regions of USA and Europe. The study objective was to determine their grain yield per plants and four morphological properties. The interactions analyses provided to select individual genotypes that are shown the highest stability and yield potential in different production conditions. Genotype with the highest yield shown lower stability level. The influence year and genotype on GYP – the grain yield per plants for investigated alfalfa genotypes was significant (Fexp=7.54* and Fexp= 8.34*). The highest average value for investigated parameter in the first research year had genotypes G53 and G44 and in second research year genotype G2. The lowest value in both research years had genotype G100 (0.28 kg ha-1 and 0.30 kg ha-1. Positive and strong correlations were found between the grain yield per plants and the number of pods per plants (r= 0.74**) and the grain yield per plant and the number of flower per inflorescence (r= 0.51*). On the basis of the obtained values, we conclude that we have excellent genotypes G2, G44, G53 and G51 for successful breeding work in order to obtain new high-yielding varieties of alfalfa.
Key words: Medicago sativa, genotypes, grain yield components, variability, correlations.
Corresponding author: Željko Lakić, PI Agricultural Institute of Republic of Srpska, Banja Luka, Bosnia & Hercegovina; and University of Banja Luka, Faculty of Agriculture, Bosnia & Hercegovina; Vera Popović, Institute of Field and Vegetable Crops, Novi Sad, Serbia; Tel.: +38766803694, E-mail: lakic.kiko@gmail.com; vera.popovic@ifvcns.ns.ac.rs
Published in „GENETIKA“ Vol. 54, No. 1 (2022), pp.15-26
© 2022Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC
575.633.11
https://doi.org/10.2298/GENSR2201015R
Original
scientific paper
IDENTIFICATION OF SUPERIOR WHEAT GENOTYPES USING MULTIPLE-TRAIT SELECTION METHODS BASED ON AGRONOMIC CHARACTERS AND GRAIN PROTEIN CONTENT UNDER RAIN-FED CONDITIONS
Mohammad Hossein ROMENA, Abdollah NAJAPHY*, Mohsen SAEIDI,
Mahmood KHORAMIVAFA
Department of Plant Production and Genetics, Faculty of Agricultural Sciences and Engineering, Razi University, Kermanshah, Iran
Abstract
Romena M. H., A. Najaphy, M. Saeidi, M. Khoramivafa (2022). Identification of superior wheat genotypes using multiple-trait selection methods based on agronomic characters and grain protein content under rain-fed conditions. - Genetika, Vol 54, No.1, 15 - 26.
Several plant breeding methods have been successfully used to improve genetic resources in many crops such as wheat. However, selection of genotypes based on multiple traits is a complex task for the breeders. The selected genotypes should display high performance in a series of desired traits. The GT-biplot and the multiple selection index have been proposed to identify a superior genotype based on various desired traits. In the present study, thirty wheat genotypes were assessed using randomized complete block design with three replications under rain-fed conditions to evaluate the genotypes by using two different multiple-trait selection methods (i.e. the GT-biplot and the multiple selection index) for agronomic traits and grain protein content. Results indicated that almost the same genotypes (G7, G9 and G16) were selected as superior entries by the both methodologies. Among the superior selected genotypes, the entries G9 (394.6 gr/m2) and G16 (388.9 gr/m2) showed higher grain yield. Furthermore, the entry G7 had the highest level of grain protein (15.91%) in the flour and the entry G18 (40.9%) revealed highest harvest index. In addition, the both methods were appropriate to identify superior wheat genotypes based on the multiple traits but the multiple selection index could be simpler and fast, if proper weights would be selected.
Key words: Genotypes x traits, GT-biplot, Multiple selection index, Selection indices, Superior wheat genotype
Corresponding author: Abdollah Najaphy, Department of Plant Production and Genetics, Faculty of Agricultural Sciences and Engineering, Razi University, Kermanshah, Iran. E-mail:
anajaphy@razi.ac.ir, nadjaphy@yahoo.com
Published in „GENETIKA“ Vol. 54, No.1 (2022), pp.27-42
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633
https://doi.org/10.2298/GENSR2201027K
Original
scientific article
PERFORMANCE OF BASMATI RICE (Oryza sativa L.) GENOTYPES UNDER DIFFERENT CROP ESTABLISHMENT METHODS
Hari KESH*1 and Khushi RAM2
1Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar-125001 (India)
2Rice Research Station, Kaul-136021, CCS Haryana Agricultural University, Hisar-125001 (India)
Abstract
Kesh H. and K. Ram (2022). Performance of basmati rice (Oryza sativa L.) genotypes under different crop establishment methods. - Genetika, Vol 54, No.1, 27 - 42.
The study was conducted to evaluate the performance of thirty-six Basmati rice genotypes for yield and its related traits under direct-seeded rice (DSR), system of rice intensification (SRI), chemical-free cultivation (CFC), and conventional transplanted rice (TPR).The genotypes were evaluated for two years at two locations in randomized block design with three replications. Genotype Pusa Basmati 1121 scored highest ~22% yield increase in system of rice intensification method over transplanted rice followed by Pusa Basmati 1 (~20%) genotypes HKR 11-447 (~18 %). Genotype Pusa Basmati 1, Pusa Basmati 1637-2-8-20-5 and Pusa Basmati1734-8-3-85 produced 8-16 % higher yield in chemical-free cultivation in comparison toTPR. Genotypes HKR 98-476, CSR 30, PAU 6297-1 yielded equally in DSR as well as in TPR. All the genotypes under study showed earlier flowering and maturity in DSR followed by SRI and TPR=CFC. Pusa Basmati 1509recordedthe earliest flowering across the environments. Most of the genotypes showed an increase in panicle weight, number oftillers per plant, number of spikelets per panicle, percent filled spikelets, Biological yield, and grain yield under SRI over other methods of rice crop establishment. Among different methods,SRI was found best than TPR, CFC, and DSR (wet).
Key words: Rice, water, direct-seeded rice, system of rice intensification, chemical-free cultivation, transplanted rice, water.
Corresponding author: Hari Kesh, Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar-125001, India, email harikeshkaul55@gmail.com
Published in „GENETIKA“ Vol. 54, No1(2022), pp. 43-62
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2201043A
Original
scientific article
MORPHOLOGICAL VARIATIONS IN QUALITATIVE AND QUANTITATIVE TRAITS OF FULANI ECOTYPE CHICKENS IN NIGERIA
Mathew Adekunle ADELEKE1, Kazeem Olajide BELLO2, Ilesanmi Oyindamola AKINYEMI 3, Oluwakemi Titilayo IREKHORE4, Babatunde Moses ILORI3, Oluwatosin Adetutu ADEYEYE4, Samuel Ayodele FAMAKINDE2
1 Discipline of Genetics, School of Life Sciences, University of KwaZulu-Natal, Westville, P/Bag X54001, Durban 4000, South Africa
2 Institute of Food Security, Environmental Resources and Agricultural Research, Federal University of Agriculture Abeokuta, PMB 2240, Abeokuta, Ogun State, 110001, Nigeria
3 Department of Animal Breeding and Genetics, Federal University of Agriculture Abeokuta, PMB 2240, Abeokuta, Ogun State, 110001, Nigeria
4Agricultural Media and Extension Centre, Federal University of Agriculture Abeokuta, PMB 2240, Abeokuta, Ogun State, 110001, Nigeria
Abstract
Adeleke M. A., K. O. Bello, I. O. Akinyemi, O. T/ Irekhore, B. M.Ilori, O. A. Adeyeye S. A. Famakinde (2022). Morphological variations in qualitative and quantitative traits of Fulani ecotype chickens in Nigeria. - Genetika, Vol 54, No.1, 43-62.
The study of Fulani ecotype (indigenous) chicken becomes necessary for proper understanding of its potentials for future breeding programmes in Nigeria. Sixty adult Fulani ecotype chickens comprising 22 males and 38 females were sourced from the Fulani kraal at Opeji in Odeda Local Government area of Ogun State and used to determine their qualitative and quantitative traits. Twenty (20) cocks out of the original 22 were selected randomly from the population for semen quality assessment. Results indicated that polydactyly was observed on 26.67% of the population. Brown and dark brown plumage colours were dominant and had an occurrence of 48.34% and 33.33%, respectively. Comb type was predominantly single (85%) while 46.67% of the population had Spur. Plumage colour influenced (p<0.05) comb length, comb width, earlobe width and tail length while shank colour had significant (p<0.05) effect on earlobe length, wattle length, wattle width, tail length. Plumage colour had significant (p<0.05-0.01) effect on sperm motility, semen pH, sperm concentration and semen volume but not (p>0.05) on livability and abnormality. Brown plumage had the highest (p<0.05) sperm motility (88.50±0.89%) and pH (7.01±0.01) which was similar to brown black. Fulani cock with light brown plumage colour had the highest (p<0.05) sperm concentration (1.90±0.11×109/ml) while dark brown had the least value 1.54±0.11× 109/ml. Light brown chicken recorded higher (p<0.05) semen volume (0.21±0.01/ml) when compared with the dark brown chicken (0.14±0.01/ml). Medium to high positive correlations were observed among all linear body measurements. The study concluded that there existed high phenotypic diversity in Fulani chickens and could be exploited for improved breeding and conservation programmes in Nigeria. The body weight and other linear body measurements of the chickens are highly and positively correlated. However, lighter plumage Fulani ecotype cocks could be adopted in multiplication program where semen motility and concentration are paramount.
Key words: Plumage colour, Body weight. Semen, Fulani chicken
Corresponding author: Bello Kazeem Olajide, Inst of Food Security, Environmental Resources & Agricultural Research (IFSERAR), University of Agriculture, P. M. B. 2240, Abeokuta, Ogun State, Nigeria, Email.kazeembello19@gmail.com
Published in „GENETIKA“ Vol. 54, No.1 (2022), pp. 63-72
© 2022Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2201063N
Original
scientific paper
GENETIC DIVERSITY OF Cordia myxa L. ASSESSED BY ISSR MARKERS
Maryam NIKKHAH, Sedigheh ARBABIAN*, Ahmad MAJD, Fariba SHARIFNIA
Department of Biology, Tehran North Branch, Islamic Azad University, Hakimieh, Tehran, Iran
Abstract
Nikkhah M., S. Arbabian, A. Majd, F. Sharifnia (2022). Genetic diversity of Cordia myxa L. assessed by ISSR markers. - Genetika, Vol 54, No.1, 63-72.
A large number of species of Cordia (Boraginaceae) are found across the tropical and subtropical parts of the planet. Cordia species are mentioned in popular medicine as being used to cure a variety of ailments affecting a variety of human organs. Population genetic structure, genetic diversity, and phenotypic variation are unknown for this species in Iran. For this reason, we collected morphological as well as molecular data on this plant species, which is significant in its own right. We employed 70 plants that were randomly picked from seven geographical communities spread throughout four provinces to conduct our research. These populations' genetic diversity variables were analyzed. The results of the structure and K-Means grouping analyses indicated the existence of five distinct gene pools in the nation, which were isolated from one another geographically. Genetic and geographical separation were shown to be correlated using the Mantel analysis. Among communities, AMOVA found considerable genetic differences, with within-population variance accounting for around 55% of the overall genetic variability. Upcoming breeding and conservation efforts for this vital medicinal plant in the nation may benefit from these findings.
Keywords: Cordia myxa L., Gene flow, Genetic admixture, ISSR, STRUCTURE analysis.
Corresponding author: Sedigheh Arbabian, Department of Biology, Tehran North Branch, Islamic Azad University, Vafadar Blv., Shahid Sadoghi st. Hakimieh, Tehran, Iran, email: arbabias@gmail.com; Sabahmaryam707@gmail.com
Published in „GENETIKA“ Vol. 54, No. 1(2022), pp. 73-89
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575. 630
https://doi.org/10.2298/GENSR2201073M
Original scientific paper
INVESTIGATION OF GENETIC PARAMETERS AND PHYTOCHEMICAL CHARACTERISTICS IN PLUM UNDER ALTITUDE CHANGE
Kerem MERTOĞLU1*
Eskisehir Osmangazi University, Faculty of Agriculture, Department of Horticulture, Eskisehir, 26160, Turkey
Abstract
Mertoğlu K. (2022). Investıgatıon of genetıc parameters and phytochemıcal characterıstıcs ın plum under altıtude change. - Genetika, Vol 54, No.1, 73-89.
Suitability of ecological factors is the most important factor affecting the productivity of agricultural activities. Sensitivity of the phytochemical characteristics that are direct the product quality to the changing environmental conditions is very high. In the present study, how climate change depending on the altitude change affects phytochemical properties in plum cultivars ‘Friar’ and ‘Fortune’ fruits which were harvested at two different altitudes (200 m and 800 m a.b.s.), during two consecutive years (2017 and 2018) was investigated. In addition, under environmental and genotype modeling, genetic parameters of the properties were determined. In line with the obtained results from the study, it was determined that the phytochemical characteristics varied parallel to the altitude change within the same latitude. Generally, amount of individual phenolic compounds and organic acids were increased with altitude increase while general phytochemical characteristics such as total phenolic content and antioxidant activity were decreased. Heritabilities of general characteristics were found lower, due to high difference between genotypic coefficient of variation (GCV) and phenotypic coefficient of variation (PCV) than organic and phenolic acids. Ranking of organic acids in both altitudes was found as malic acid> acetic acid> oxalic acid> ascorbic acid in both cultivars. In terms of phenolic acids, a stable order could not be determined while chlorogenic acid has come to the front in both cultivars. Since cultivar/location interactions found significant according to bi-plot segregation, investigating the changes at genotype level would be more accurate.
Keywords: Heritability, phenolic compounds, organic acids, altitude
Corresponding author: Kerem Mertoglu, Eskisehir Osmangazi University, Faculty of Agriculture, Department of Horticulture, Eskisehir, 26160, Turkey, E-mail: kmertoglu@ogu.edu.tr, tel:+902223242991
Published in „GENETIKA“ Vol. 54, No1(2022), pp 91-108
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2201091S
Original
scientific paper
GENETIC VARIATION IN MINERAL PROFILES, YIELD CONTRIBUTING AGRONOMIC TRAITS, AND FOLIAGE YIELD OF STEM AMARANTH
Umakanta SARKER1*, Md. Golam AZAM2, Md. Zahirul Alam TALUKDER2
1Department of Genetics and Plant Breeding, Faculty of Agriculture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh
2Plant Breeding Division, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh
Abstract
Sarker U., Md. G. Azam, Md. Z. A. Talukder (2022). Genetic variation in mineral profiles, yield contributing agronomic traits, and foliage yield of stem amaranth. - Genetika, Vol 54, No.1, 91-108.
The study was evaluated to estimate mineral profiles and find out selection criteria for high foliage yielding cultivars by assessing the magnitude of genetic variability, heritability, genetic advance, association, and contribution of characters of sixteen stem amaranth genotypes in a randomized block design with three replications. Based on high heritability and high GA and GAPM and close values between Vg vs. Vp and GCV vs. PCV, all the traits could be selected to improve the foliage yield of stem amaranth. A significant and desirable positive correlation was observed for Fe, Ca, Mn, K, and leaves plant-1 both at genotypic and phenotypic levels. These five traits could be selected for improving the foliage yield of stem amaranth as these traits exhibited less influence on the environment. The insignificant genotypic correlation values were observed between mineral vs. mineral and mineral vs. foliage yield and yield contributing agronomic traits, which indicate that selection for high mineral content might be possible without compromising yield loss. A high to moderate direct effect along with a significant correlation was found in Fe, K, Ca, and Mn. Fe, K, Ca, and Mn had the most significant contribution to foliage yield of stem amaranth as these traits exhibited considerable positive direct effects and significant correlation coefficients on foliage yield. The accessions AS7, AS4, AS5, AS14, and AS16 had high foliage yields containing moderate to high mineral profiles. These five accessions could be selected as high-yielding cultivars. Selection could be made based on Fe, K, Ca, and Mn content to improve stem amaranth. The accessions AS7, AS4, AS5, AS14, and AS16 could be used as high foliage yielding and mineral profiles enrich cultivars.
Keywords: mean, genotypic and phenotypic variances, genotypic and phenotypic coefficient of variations, heritability, genetic advance, correlation, and path coefficients.
Corresponding author: Umakanta Sarker, Department of Genetics and Plant Breeding, Faculty of Agriculture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh. Postal Code 1706. Phone: +880-1716606098, Email: umakanta@bsmrau.edu.bd
Published in „GENETIKA“ Vol. 54, No.1 (2022), pp.109-118
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575. 630
https://doi.org/10.2298/GENSR2201109U
Original scientific paper
IDENTIFICATION OF GENETIC DIVERSITY IN WILD PEAR (Pyrus Elaeagrifolia Pall.) GENOTYPES COLLECTED FROM DIFFERENT REGIONS OF TURKEY
WITH SSR MARKER SYSTEM
Aydın UZUN1, Hasan PINAR1, Mehmet YAMAN1*, Merve A. YIGIT1, Yaşar CAKIROGLU2, Arif KARAKAYA2, Mehmet UYSAL2, Gökhan OZTURK3, Kadir Uğurtan YILMAZ4, Kahraman GURCAN5,
Sezai ERCISLI6
1Erciyes University Department of Horticulture, Kayseri, Turkey
2Ministry of Agriculture and Forestry General Directorate of Combating Desertification and Erosion, Ankara, Turkey
3Fruit Research Institute, Egirdir, Isparta, Turkey
4Sütçü İmam University Department of Horticulture, Kahramanmaras, Turkey
5Erciyes University Department of Agricultural Biotechnology, Kayseri, Turkey
6Ataturk University Department of Horticulture, Erzurum, Turkey
Abstract
Uzun A., H. Pinar, M. Yaman, M. A. Yigit, Y. Cakiroglu, A. Karakaya, M. Uysal, G. Ozturk, K.r U. Yilmaz, K. Gurcan, S. Ercisli (2022). Identification of genetic diversity in wild pear (Pyrus elaeagrifolia pall.) genotypes collected from different regions of Turkey with SSR marker system. - Genetika, Vol 54, No.1, 109-118.
Turkey with diverse ecologies is among the unique countries in terms of plant species and diversity. Among these plant species, naturally growing wild pears (Pyrus elaeagrifolia Pall.) are resistant to chlorosis and drought and could be used in rootstock development programs. In present study, genetic diversity in 96 wild pear genotypes collected from 11 different provinces (Kayseri, Ankara, Kahramanmaraş, Adana, Nevşehir, Konya, Isparta, Denizli, Uşak, Afyonkarahisar, Eskişehir) and regions of Turkey through selection was investigated with the use of SSR (Simple Sequence Repeat) molecular marker system. Present analyses carried out in ABI (Applied Biosystem) 3500 capillary electrophoresis system revealed 93 scorable and all polymorphic bands, thus polymorphism rate was 100%. In UPGMA (Unweighted pair group method with arithmetic mean) dendrogram of wild pear genotypes, similarity index values varied between 0.20-0.83 and a large variation was observed among the genotypes. Present finding may have significant contributions to further studies to be conducted for preservation of gene sources and breeding of wild pear genotypes.
Keywords: Wild pear, Pyrus elaeagrifolia Pallas, molecular marker, genetic diversity
Corresponding author: Mehmet Yaman, Erciyes University Department of Horticulture, Kayseri, Turkey, E-mail: mhmt.-07@hotmail.com, Tel: +90 5387736019
Published in „GENETIKA“ Vol. 54, No. 1(2022), pp. 119-130
© 2022 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2201119Z
Original
scientific paper
GENETIC DIVERSITY AND INTER-RELATIONSHIP AMONG Stellaria L. (CARYOPHYLLACEAE) SPECIES BY ISSR MARKERS
Yan ZHOU and Zhongbiao ZHENG,*
School of Intelligent Construction, Luzhou vocational and technical college, Luzhou 646000, Sichuan, China
Abstract
Zhou Y. and Z. Zheng (2022). Genetic Diversity and inter-relationship among Stellaria L. (Caryophyllaceae) species ISSR markers. - Genetika, Vol 54, No.1, 119-130.
Stellaria L. (Caryophyllaceae, Alsinoideae) comprises ca. 150–200 species across the world. Stellaria species are common herbs, preferred humid mountainously slopes, but some grew in desert. Main center of diversification for Stellaria is Eurasia, with a center of distribution in the mountains of E. central Asia. Therefore, due to the importance of these plant species, we performed a molecular data analysis for this genus. For this study, we used 65 randomly collected plants from six species in six provinces. Amplification of genomic DNA using 5 primers produced 70 bands, of which 62 were polymorphic (84.00%). The obtained high average PIC and MI values revealed high capacity of ISSR primers to detect polymorphic loci among Stellaria species. The genetic similarities of six collections were estimated from 0.69 to 0.85. According to Inter-Simple sequence repeats (ISSR) markers analysis, S. pallida and S. graminea had the lowest similarity and the species of S. media and S. pallida had the highest similarity. The aims of present study are: 1) can ISSR markers identify Stellaria species, 2) what is the genetic structure of these taxa in Iran, and 3) to investigate the species inter-relationship? The present study revealed that ISSR markers can identify the species.
Keywords: Iran, Species Identification; Structure, Stellaria species, ISSR markers
Corresponding author: Zhongbiao Zheng, School of Intelligent Construction, Luzhou vocational and technical college, Luzhou 646000, Sichuan, China, E-mail: zzb197910@126.com
Published in „GENETIKA“ Vol. 54, No.1 (2022), pp. 131-145
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC
575.630
https://doi.org/10.2298/GENSR2201131K
Original
scientific paper
GENETIC DIVERSITY OF WHITE MULBERRY (Morus alba L.) ACCESSIONS SELECTED FROM NORTH EASTERN TURKEY
Salih KESKIN1, Yaşar AKCA2, Sezai ERCISLI3*
1Republic of Turkey, Ministry of Agriculture and Forestry, Erzincan Horticultural Research Institute, 24060 Erzincan, Turkey
2 Department of Horticulture, Faculty of Agriculture, Tokat Gaziosmanpasa University, 60240 Tokat, Turkey
3Department of Horticulture, Faculty of Agriculture, Ataturk University 25240 Erzurum, Turkey
Abstract
Keskin S., Y. Akca, S. Ercisli (2022). Genetic diversity of white mulberry (Morus alba l.) accessions selected from north eastern Turkey. - Genetika, Vol 54, No.1, 131-145.
This study was carried out between 2011-2013 years, on the mulberry population of Gümüşhane province and aimed to select promising white mulberry (Morus alba) genotypes according to main mulberry breeding criteria. Fruit samples were taken from 62 white mulberry genotypes in the first year and 54 in the second year. In fruit samples of selected promising white mulberry genotypes pomological and technological traits were investigated. Results indicated high diversity among selected genotypes for most of the pomological and technological traits. The average fruit weight ranged from 1.41 g (KU18) to 5.47 g (GUM23); Soluble Solid Content (SSC) from 10.07% (TO23) to 26.60% (GUM20); taste and aroma scores from 2.56 (GUM1) to 10.00 (TO29); fruit juice yield from 47.70% (GUM 20) to 92.44% (TO26); dried fruit yield from 11.99% (TO31) to 30.93% (TO23); fruit diameter from 11.25 mm (TO5) to 18.23 mm (KU7); fruit length from 17.84 mm (KU21) to 33.95 mm (KU7); stalk length from 4.41mm (GUM17) to 16.10 mm (GUM20), respectively. After assessment at the end of the study, 9 genotypes were found suitable for table consumption, 10 genotypes for molasses processing and 13 genotypes for drying.
Keywords: Mulberry (Morus alba), Fruit traits, Genetic resources, Selection.
Corresponding author: Sezai Ercisli, Department of Horticulture, Faculty of Agriculture, Ataturk University 25240 Erzurum, Turkey, E-mail: sercisli@gmail.com
Published in „GENETIKA“ Vol. 54, No. 1(2022), pp. 147-160
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2201147H
Original
scientific paper
DETERMINATION OF MUTAGENIC-SENSITIVITY AND INDUCED VARIABILITY IN THE MUTANT POPULATIONS OF ‘BACARDI’ CHRYSANTHEMUM CULTIVAR
Gulden HASPOLAT1, Burak KUNTER2 and Yaprak KANTOGLU2
1Aegean Agricultural Research Institute, Cumhuriyet Street, No:138, Mail Box: 9, 35661, Menemen, Izmir, Turkey
2Turkish Energy, Nuclear and Mineral Research Agency, Nuclear Energy Research Institute, Atom Street No: 27, 06983, Saray, Ankara, Turkey
Abstract
Haspolat G., B. Kunter, Y. Kantoglu (2022). Determination of mutagenic-sensitivity and induced variability in the mutant populations of ‘Bacardi’ chrysanthemum cultivar. - Genetika, Vol 54, No.1, 147-160.
Mutation breeding is one of the most important breeding method for ornamental plants. Chrysanthemum is the genus that has the richest mutant varieties in ornamental plants. The objective of this study is to create variation by gamma irradiation and improving traits by mutation breeding. For this aim, in vitro bud explants of white Bacardi variety were irradiated by gamma radiation at 20 Gy (Gray). In vitro subcultures were continued until M1V4 period and observations were obtained in this period. Some changes were observed on heights and flowers of the plants such as; variable flowers, flowering time, differentiation on plant length, flower number per bunch and ray floret differentiations. The changes of the ray florets were determined as color changes to pink and yellow. Mutation frequency was calculated by 1.1% of the population. Approximately 0.9% of useful mutant lines determined from the selected mutants.
Keywords: Chrysanthemum, in vitro mutation, effective mutation dose (EMD-LD50), mutation frequency
Corresponding author: Gulden Haspolat, Aegean Agricultural Research Institute, Cumhuriyet Street, No:138, Mail Box: 9, 35661, Menemen, Izmir, Turkey, guldenhaspolat@gmail.com
Published in „GENETIKA“ Vol. 54, No1 (2022), pp161-172
© 2022 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575. 630
https://doi.org/10.2298/GENSR2201161G
Original
scientific paper
MULTI-ENVIRONMENT CHARACTERIZATION OF BREAD WHEAT GENOTYPES FOR WATERLOGGING TOLERANCE
Vikas GUPTA*, Pradeep KUMAR, Gyanendra SINGH, Charan SINGH, Amit Kumar SHARMA, BS TYAGI,
GP SINGH
Division of Crop Improvement, ICAR-Indian Institute of Wheat and Barley Research,
Karnal - 132001 (Haryana), India
Abstract
Gupta V., P. Kumar, G. Singh, C. Singh, A. K. Sharma, BS Tyagi, GP Singh (2022). Multi-environment characterization of bread wheat genotypes for waterlogging tolerance - Genetika, Vol 54, No.1, 161-172.
The improvement in waterlogging tolerance of wheat may help to enhance the productivity of crop in regions having high and untimely rainfalls with poor drainage. The present study was undertaken to characterize waterlogging tolerance of 65 different bread wheat genotypes. The traits like grain yield, biological yield, tillers per meter, grain filling duration, spike weight and plant height under waterlogged conditions were found sensitive. The spike weight, tillers per meter, 1000 grain weight, biological yield and harvest index showed significant correlations with grain yield under both normal and waterlogged conditions. Waterlogging tolerance index (WTI) showed positive correlation with plant height, tillers per meter, biological yield, thousand grain weight and grain yield. These agronomic traits along with WTI were used to identify tolerant genotypes with high yield potential. Wheat genotypes SSDC3-264, SSDC3-347, NW 5054 and PBW 550 were identified as tolerant and could be utilized for improving the waterlogging tolerance of wheat.
Keywords: wheat, waterlogging tolerance index, correlation, agronomic traits
Corresponding author: Vikas Gupta, Division of Crop Improvement, ICAR-Indian Institute of Wheat and Barley Research, Karnal - 132001 (Haryana), India. E-mail: vikas.gupta@icar.gov.in
Phone: 0182-2209128
Published in „GENETIKA“ Vol. 54, No. 1(2022), pp. 173-186
© 2022Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2201173Y
Original scientific paper
EVALUATION OF GENETIC VARIABILITY Rindera USING RAPD MARKERS
Juan YIN*
Forestry College, Xinyang College of Agriculture and Forestry, Xinyang, Henan, 464000, China
Abstract
Yin J. (2022). Evaluation of genetic variability Rindera using RAPD markers. - Genetika, Vol 54, No.1, 173-186.
Genetic diversity investigations are critical for understanding how to conserve and manage plant resources in every setting. Six Rindera species are reported in Iran. No detailed Rindera genetic diversity was investigated using Random Amplified Polymorphic DNA (RAPD) experiments. Six plants from Iran's seven provinces were gathered and studied for our scientific purposes. Seventy plant specimens have been gathered in total. Our objectives were as follows: 1) to determine genetic variability among Rindera species, and 2) is there a correlation between the genetic and geographical distance of the species? 3) Populations and taxon genetic structure we revealed that quantitative morphological features varied significantly across plant species. Rindera species were classified into two groups using the unweighted pair group approach with arithmetic mean and principal component analysis. The unbiased expected heterozygosity (UHe) of Rindera Regia was in the 0.18 range. Rindera lanata has important Shannon information (0.30). The lowest value was 0.22 for Rindera Regia. In R. cyclodonta and Rindera media, the observed number of alleles (Na) varied between 0.33 and 0.49. R. cyclodonta and Rindera bungei had Ne values between 1.034- 1.17, indicating an effective number of alleles. Rindera has a comparatively low gene flow (Nm) (0.45). According to the Mantel test, there was a significant correlation (r = 0.33, p=0.0001) between genetic and geographical distances. We identified a substantial level of genetic variation, which demonstrates that the Rindera species can adapt to altering environments because genetic diversity is associated with species adaptability. The current findings indicated the efficacy of RAPD markers and morphometry approaches for studying genetic variation in Rindera species.
Keywords: Gene flow, Random Amplified Polymorphic DNA (RAPD) Rindera, isolation, morphometry
Corresponding author: Juan Yin, Forestry College, Xinyang College of Agriculture and Forestry, Xinyang, Henan, 464000, China; E-mail: yinjuan20210313@163.com
Published in „GENETIKA“ Vol. 54, No.1(2022), pp. 187-205
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.11
https://doi.org/10.2298/GENSR2201187K
Original scientific paper
GENETIC DIVERSITY FOR MINERAL ELEMENT IN SEEDS OF TURKISH OAT LANDRACES
Özge Doğanay ERBAŞ KÖSE1*, Zeki MUT1, Hasan AKAY2
1Bilecik Şeyh Edebali University, Faculty of Agriculture and Natural Sciences, Department of Field Crops, TR-11230, Bilecik, Turkey
2Ondokuz Mayıs University, Bafra Vocational School, Department of Plant and Animal Production, TR-55400 Samsun, Turkey
Abstract
Köse Erbaş D. Ö., Z. Mut, H. Akay (2022). Genetic diversity for mineral element in seeds of Turkish oat landraces. - Genetika, Vol 54, No.1, 187-205.
Oat grain is an important cereal in the human diet and as an animal feed. It is cultivated in many regions of the world. This study was carried out to determine the variability in the element content of these oat gene sources collected from the West and Middle Black Sea Region of Turkey. Two hundred and fifty-six oat genotypes were examined under this study. The mean of mineral elements in landraces and commercial cultivars were 40.39 g kg-1 and 36.16 g kg-1 for K, 23.90 g kg-1 and 28.51 g kg-1 for P, 12.47 g kg-1 and 10.99 g kg-1 for Mg, 10.92 g kg-1 and 10.96 g kg-1 for Ca, 2.58 g kg-1 and 2.36 g kg-1 for Na, 54.70 mg kg-1 and 53.02 mg kg-1 for Fe, 32.08 mg kg-1 and 23.51 mg kg-1 for Mn, 25.39 mg kg-1 and 24.05 mg kg-1 for Zn and 8.26 mg kg-1 and 8.07 mg kg-1 for Cu, respectively. The ranges of mineral elements in the landraces were significantly higher than those in the commercial cultivars. The contributions of the first and second principal components (PC1 and PC2) to the total variation were 43.0% and 15.6%, respectively. These results indicate that examined oat landraces can provide a good source of diversity in mineral elements concentration and could be successfully used in biofortification programs.
Key words: Avena sativa L., macro-micro elements, genotypes, local cultivar
Corresponding author: Özge Doğanay Erbaş Köse, Bilecik Şeyh Edebali University, Faculty of Agriculture and Natural Sciences, Department of Field Crops, TR-11230, Bilecik, Turkey, e-mail: ozgedoganay.erbas@bilecik.edu.tr, Tel: +902282141251 Fax: +902282141251
Published in „GENETIKA“ Vol. 54, No. 1(2022), pp. 207-217
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2201207A
Original scientific paper
SOMACLONAL VARIATION IN POMEGRANATE (Punica granatum L.):
ISSR AND CYTOLOGICAL EVIDENCES
Seyed Ardalan AZIZI1, Farah FARAHANI2*, Masoud SHEIDAI3
1Department of Genetic, Qom Branch, Islamic Azad University, Qom, Iran
2Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran
3Department of Biology, Faculty of Biological Sciences, Shahid Beheshti University, Evin, Tehran, Iran
Abstract
Azizi S. A., F. Farahani, M. Sheidai (2022). Somaclonal variation in pomegranate (Punica granatum L.): ISSR and cytological evidences. - Genetika, Vol 54, No.1, 207-217.
New advancements in tissue culture techniques have resulted in vitro propagation of several regenerations of horticultural crops. The somaclonal variation which occurs during plant tissue culture is a potential source for inducing new genetic variability in such crops. Several approaches have been utilized to ascertain the occurrence of somaclonal variation. The pomegranate plant can adapt to various environmental conditions and grow well in different kinds of soil and climate. Pomegranate Zagheh cultivar is mainly cultivated in the state of Esfehan, Kashan city in Iran. The present study was performed to investigate the potential occurrence of somaclonal variation in tissue culture regenerated plants of the cultivar Zagheh and provide morphological, cytological and molecular evidences for its occurrence. Nodal segments were chosen for the in vitro propagation of pomegranate. Explants were cultured on MS basal medium. We carried out both molecular (ISSR) and karyological studies in propagation collected. In the present study, we observed variation in both morphological (length of shoot, branch, internode, leaf and root and number of branch and root) and genetic features of the tissue culture regenerated plants. The regenerated plants of the third sub-culture differed from mother plants in the ISSR marker. We observed frequent occurrence of cells with different chromosome numbers, ranging from normal diploid 2n = 2x = 16 to tetraploid 2n = 32 chromosome number.
Keywords: Somaclonal variation, pomegranate, ISSR, cytological study
Corresponding author: Farah Farahani, Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran. E-mail: farahfarahani2000@yahoo.com, Tel: +98912-2778171
Published in „GENETIKA“ Vol. 54, No. 1(2022), pp. 219-232
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2201219A
Original
scientific paper
EVALUATION OF NEW TURKISH APRICOT GENETIC RESOURCES
FROM THE IRANO-CAUCASIAN ECO-GEOGRAPHICAL GROUP
Bayram Murat ASMA1, Fırat Ege KARAAT2*, Çiğdem ÇUHACI1
1Malatya Turgut Özal University, Faculty of Agriculture, Department of Horticulture, Malatya, Turkey
2Adıyaman University, Faculty of Agriculture, Department of Horticulture, Adıyaman, Turkey
Abstract
Asma M. B., F. E. Karaat, Ç. Çuhaci (2022). Evaluation of new Turkish apricot genetic resources from the Irano-Caucasian eco-geographical group. - Genetika, Vol 54, No.1, 219-232.
This study presents 19 characters of 86 apricot cultivars and genotypes of the Irano-Caucasian eco-geographical group evaluated using principal component analysis. The high variability and differences among the apricot genotypes in terms of morphological, phenological and fruit quality traits were defined. The fruit size of the genotypes was generally very small (9.3 %) or small (43.0 %), the total rate of big and very big fruit genotypes was only 16.3%. The data showed that 90.1 % of the genotypes had yellow ground fruit colour, 88.4% had sweet kernel and 65% had firmness ≥5 kg/cm2. About half of the apricot genotypes have 20% or high total soluble solids content. Most of the genotypes (67.3%) were harvested in mid-season and other genotypes (23.3%) were harvested early, while 4.7% of them were harvested very late, 3.5 % of the genotypes late. Only one genotype (1.2%) was harvested very early. The fruit size was highly correlated with fruit weight, pit weight and fruit flesh/pit rate. The same correlation was also observed between the fruit ground colour and fruit flesh colour. On the other hand, the total soluble solids were moderately correlated with fruit flesh firmness and seed taste. The results of the principal component analysis show that the 55% of the total variation is represented for the first three main components (22.9, 19.8, and 12.3%, respectively). The germplasm presented a large variation in the evaluated characters and most of the genotypes were found having high total soluble solids and low titratable acidity which would be beneficial for future breeding programs held to improve the related characters.
Keywords: Apricot, fruit quality, germplasm, principal component analysis, Prunus armeniaca L.
Corresponding author: Fırat Ege Karaat, Adıyaman University, Faculty of Agriculture, Department of Horticulture, Adıyaman, Turkey, email:fkaraat@adiyaman.edu.tr, +905067430932
Published in „GENETIKA“ Vol. 54, No. 1(2022), pp.233-254
© 2022 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2201233K
Original scientific paper
GGE BIPLOT ANALYSIS FOR SEED PRODUCTION POTENTIAL OF EUROPEAN CARROT (Daucus carota L.) GENOTYPES IN PLAINS AND SUBMOUNTENOUS ZONES OF PUNJAB
Karamvir KAUR1*, Tarsem Singh DHILLON1, Rajinder SINGH2
1Department of Vegetable Science, Punjab Agricultural University Ludhiana, Punjab
2Punjab Agricultural University Ludhiana, Punjab
Abstract
Kaur K., T. Singh Dhillon, R. Singh (2022). GGE biplot analysis for seed production potential of European carrot (Daucus carota L.) genotypes in plains and submountenous zones of Punjab - Genetika, Vol 54, No.1, 233-254.
Carrot (Daucus carota L.) is grown worldwide in two different groups namely Asian and European. However the seed production of European cultivars is not possible in tropical and sub-tropical areas with less chilling period. The present study was planned to find the suitable areas in Punjab near foothills of Himalaya for seed production of European cultivars. Therefore fifteen carrot genotypes stecklings were transplanted in three dates of sowing i.e. first week of December, first week of January and first week of February at two locations of Punjab namely Ludhiana and Langroya. Seed yield traits like number of branches, number of seeds per plant, seed weight per plant, 100 seed weight and seed yield per hectare were recorded. Combined analysis of variance showed highly significant differences for GE (genotype×environment) interaction indicating the possibility of stable genotypes. Seed production was better in location Langroya and better than location Ludhiana. Most of the genotypes showed stable performance with regard to seed yield and yield parameters in first week of December and first week of January by GGE Biplot. Number of branches were significantly higher in genotype Pusa Meghali which was statistically at par with P-3, PCO-4. Similarly PCO-4 showed maximum number of seeds per plant and PCO-6 showed the highest seed weight per plant and 100 seed weight. Moreover, seed yield per hectare for genotypes PCO-4, PCO-6 was significantly higher than other genotypes. The study showed that European cultivars of carrot i.e. Pusa Meghali, PCO-4 and PCO-6 cultivars can produce seed in submontaneous zones of Punjab.
Keywords: Biplot, Carrot, European, Genotypes, chilling requirement, Submontaneous
Corresponding author: Karamvir Kaur, Department of Vegetable Science, Punjab Agricultural University, Ludhiana, Punjab, E-mail: karamvir476@gmail.com
Published in „GENETIKA“ Vol. 54, No. 1(2022), pp255-264
© 2022 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.11
https://doi.org/10.2298/GENSR2201255B
Original
scientific paper
THE EFFECTS OF IRRIGATION WATER SALINITY ON THE SEED GERMINATION AND SEEDLING GROWTH OF RICE
Fikri BAYINDIR a, Yalçın COŞKUN b
a:Department of Field Crops, Çanakkale Onsekiz Mart University, Terzioglu Campus Area, Çanakkale, Turkey
b:Department of Plant Production and Animal Husbandry, Lapseki Vocational College, Çanakkale Onsekiz Mart University, Lapseki Campus Area, Çanakkale, Turkey
*: Developed from Fikri Bayindir’s MSc. thesis
Abstract
Bayindir F., Y. Coşkun (2022). The effects of irrigation water salinity on the seed germination and seedling growth of rice- Genetika, Vol 54, No.1, 255-264.
To determine the effects of irrigation water salinity on seed germination and seedling development of two rice cultivars, irrigation waters with low SAR (below 3) were prepared in different salinity levels (control [0.5], 2, 4, 8, 12, 16, and 20 dS m-1) from different salinity sources (NaCl, MgS04, and CaCl2 salts). The average germination rate was decreased for the cv. Baldo 20.19%, while for cv. Osmancık - 97 it was 26.73%. The average of the single seedling dry weight of cv. Baldo was 0.2666 g, while for cv. Osmancık-97 it was 0.2569 g. The average single seedling dry weight was 0.2940 g in the control application. In parallel to the increased irrigation water salinity level, the single seedling dry weight decreased. The irrigation water salinity had not affected up to 4 dS m-1 in terms of the germination rate decrease and the single seedling dry weight of rice, but it started to be affected when increased salinity level to 8 dS m-1 and it was more effective at higher doses. Also, cv. Osmancık-97 was more sensitive to irrigation water salinity than cv. Baldo. In conclusion, it turns out that the irrigation water to be used in rice farming should not have salinity since the tolerance of the rice plant against irrigation water salinity is low.
Keywords: rice, germination rate, seedling development, irrigation water salinity, SAR
Corresponding author: Yalçın Coşkun, Department of Plant Production and Animal Husbandry, Lapseki Vocational College, Çanakkale Onsekiz Mart University, Lapseki Campus Area, Çanakkale, Turkey,ycoskun33@hotmail.com +905053121767
Published in „GENETIKA“ Vol. 54, No. 1(2022), pp. 265-274
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575. 630
https://doi.org/10.2298/GENSR2201265K
Original
scientific paper
ASSESSMENT OF EFFICIENCY OF BREEDING METHODS USING MOLECULAR MARKERS IN SOYBEAN
Gaurav KHOSLA, B S GILL, Asmita SIRARI, Pritpal SINGH
Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, India
Abstract
Khosla G., B S Gill, A. Sirari, P. Singh (2022). Assessment of efficiency of breeding methods using molecular markers in soybean. - Genetika, Vol 54, No.1, 265-274.
Four breeding methods viz. pedigree method (PM), single pod descent (SPD), single pod descent with selection (SPDS) and bulk method (BM) were compared for maintaining variability in the population in advanced generations using simple sequence repeats (SSR) markers. The F4:7 lines advanced through different breeding methods from six different crosses were evaluated for number of unique lines retained in each method at a similarity coefficient ≥ 0.875. Eighteen polymorphic SSR markers were used for estimating similarity coefficient between lines within a breeding method in each cross. In all the crosses, SPD method was the best method in producing unique lines with a range from 42.9 to 100 per cent. SPD method had also the least number of lines pairing with two or more lines. PM and BM had the least number of unique lines in three crosses each and also maximum proportion of lines produced by these two methods were paired with four or more lines. Thus, SPD method was the most efficient among these four methods in retaining the variability in a population, but the breeder has to make a choice between high variability and comparative harvest and seed processing efficiencies to select the most suitable breeding method.
Keywords: Soybean, Breeding methods, Similarity coefficient, SSR markers
Corresponding author: Gaurav Khosla, Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, India, E-mail: goruvkhosla@gmail.com, tel:+9198159-65404
Published in „GENETIKA“ Vol. 54, No. 1(2022), pp. 275-288
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2201275T
Original
scientific paper
POPULATION DIFFERENTIATION AND GENE FLOW OF Glaucium flavum (Papaveraceae)
Song TING1,2 and Yu YIBING1*
1School of Foreign Language, Chizhou University, Chizhou City, China
2School of Humanities,Tongji University, Shanghai City, China
Abstract
Ting S. and Y. Yibing (2022). Population differentiation and gene flow of Glaucium flavum (Papaveraceae). - Genetika, Vol 54, No.1, 275-288.
Yellow hornpoppy (Glaucium flavum Crantz.) is a herbaceous plant with gray-green leaves in coastal sands, rocky areas, and heavily eroded soils up to 500 meters above sea level. Glaucium flavum is native to Northern Africa, temperate zones in Western Asia and Europe, and is indigenous to Iran. The plant has been widely recognized for its aporphine-type isoquinoline alkaloids, which are pharmacologically active. Thus, we conducted a combination of morphological and molecular data analysis on such species because of the plant species' relevance. One hundred seven randomly collected plants from 14 natural populations in 5 provinces were evaluated using ISSR markers and morphological traits. The evaluation of molecular variance (AMOVA) demonstrated significant genetic divergence between the examined populations. It indicated that 25% of overall genetic variability was related to intra-population variety, whereas 75% was due to inter-population genetic differentiation. ISSR primers discovered 156 bands, 139 (83 %) of which have been polymorphic, each primer containing an average of 13 bands. The Polymorphic Bands (PPB) Percentage (ISSR-6) varied from 50% to 100%. (ISSR-1, ISSR-4, and ISSR-5). The average polymorphic information content (PIC), Shannon's information indexes (I), and several effective alleles (Ne) were correspondingly 0.39, 0.26, and 1.2.
Keywords: Genetic diversity, Gene flow, Genetic differentiation, inter simple sequence repeat (ISSR)
Corresponding author: Yu Yibing, School of Foreign Language, Chizhou University, Chizhou City, China, email:pinger4598@163.com
Published in „GENETIKA“ Vol. 54, No. 1(2022), pp. 289-306
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC575.633.15
https://doi.org/10.2298/GENSR2201289G
Original
scientific article
INFLUENCE OF MAIZE HYBRIDS AND APPLAYED INSECTICIDES
ON Ostrinia nubilalis HBN. ATTACK
Dragan GRČAK1, Snežana GOŠIĆ DONDO2, Milosav GRČAK1, Danijela RISTIĆ2,
Danijela KONDIĆ3, Djuradj HAJDER3, Željko POPOVIĆ4, Desimir KNEŽEVIĆ1
1University of Priština, Faculty of Agriculture, Kosovska Mitrovica–Lešak, Serbia
2Maize Research Institute “Zemun Polje”, Belgrade, Serbia
3University of Banja Luka, Faculty of Agriculture, Banjaluka, Republika Srpska,
Bosnia & Herzegovina
4University of Novi Sad, Faculty of Science, Novi Sad, Serbia
Abstract
Grčak D., S. Gošić Dondo, M. Grčak, D. Ristić, D. Kondić, D. Hajder, Ž. Popović, D. Knežević (2022). Influence of maize hybrids and applayed insecticides on Ostrinia nubilalis HBN. attack. - Genetika, Vol 54, No.1, 289-306.
Ostrinia nubilalis Hbn. is a corn pest that can do great damage to plants in some seasons. The aim of this paper is to study the resistance / susceptibility of hybrids to the attack of European corn borer and the effects of different doses of insecticides and time of application in protection against pests on maize plants, but primarily the influence of hybrids on attack intensity. The six maize hybrids of different maturation groups (ZP 427, ZP 434, ZP 555, ZP 600, ZP 606 and ZP 666) used for study effect of attack of Ostrinia nubillalis Hbn., on control variant T1 without application of insecticide and on variant treated with two insecticides Fobos EC (bifentrin - 200 ml ha-1) and combination of Match 050 EC and Nurelle D (lufenuron 50 g l-1 and hlorpirifos 500 g l-1 + cipermetrin 50 g l-1), 0.75 l ha-1) in two terms (in the first generation of insect flying T2 and T3 and in the second generation of insect flying T4 and T5) in three replication. The results showed the highest intensity of Ostrinia nubilalis Hbn. attack and degree of damages on control variant and significant differences between treatments for investigated parameters. Observing all hybrids and treatments, there was no statistical significance between the six examined maize hybrids of different maturity groups. In the control variant, the greatest attack (damage) was in plants, namely in the hybrid ZP 666 (94.28%) and ZP 606 (93.90%) and among the variants of applied insecticides, the greatest damage was found in the treatment T5 with the applied combination of Match + Nurelle in hybrid ZP 555 and was 92.02%. On treatment T1-control variant without insecticides, the smallest attack with Ostrinia nubilalis Hbn. was found in the hybrid ZP 434 (from 88.76%) and among the variants of applied insecticides, the smallest attack was found on variant T2 with applied Phobos in the first generation of insect in hybrid ZP 434 and amounted to 77.12%. The attack of Ostrinia nubilalis Hbn. and variation of average mass of maize cob varied in dependence of maze of hybrids and variant of insecticides application.
Keywords: attack, hybrid, insecticide, maize, Ostrinia nubilalis
Published in „GENETIKA“ Vol. 54, No1(2022), pp.307-328
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2201307S
Original
scientific paper
GENETIC AND PHENOTYPIC CORRELATION COEFFICIENTS
OF QUANTITATIVE TRAITS IN TWO CHICKEN GENOTYPES
A.J. SANDA1, M.N. BEMJI1, M. WHETO1, A.O. OSO2, M.O. SANDA1, O. OLOWOFESO1
1Department of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria.
2Department of Nutrition, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria.
Abstract
Sanda A.J., M.N. Bemji, M. Wheto, A.O. Oso, M.O. Sanda, O. Olowofeso (2022). Genetic and phenotypic correlation coefficients of quantitative traits in two chicken genotypes. - Genetika, Vol 54, No.1, 307-328.
This study was carried out to estimate genetic and phenotypic correlations of morphometric traits of a newly developed Nigerian poultry breed (FUNAAB Alpha) and Noiler chickens. A total of 300 unsexed day-old-chicks comprising 150 each of the two genotypes were raised for eighteen weeks. Superior individuals (4 cocks and 20 hens per genotype) from first phase were selected based on weight to constitute parents of 100 birds per genotype raised in second phase for 12 weeks. Body weight, and linear body measurements (body circumference, breast girth, thigh length, shank length and wing length) were taken on weekly basis. Growth data were analysed using Generalized Linear Model of SAS and least significant difference (LSD) test was used to separate significant means for the genotypes. Computed variances and covariances (PROC MIXED DATA) were used to estimate genetic and phenotypic correlations. Results showed that Noiler chicken had higher (p<0.05) body weight and linear body measurements from 10 to 18 weeks with average weight of 2079.55 g compared with 1895.29 g for FUNAAB Alpha. Positive genetic correlations were observed among the traits of interest in both FUNAAB Alpha and Noiler with the latter having higher genetic correlation coefficients which ranged between 0.573 (TL x SL) and 0.953 (BG x BC) at week 12. Sexual dimorphism favoured male birds in all the traits measured. The implication of these results is that it is important to know both the effect of the trait actually being selected and its effect on the other traits. Genetic correlations result from pleiotropic effects of genes on multiple traits or from chromosomal linkage of genes affecting different traits. It estimates the degree to which the traits studied are affected by the same genes (Pleiotropic) or pairs of genes.
Keywords: Chicken, genotype, morphological traits, correlation
Corresponding author: Sanda Adeyinka Julius, Department of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria, E-mail: sandaaj@funaab.edu.ngTelephone: +2348038487865
Published in „GENETIKA“ Vol. 54, No. 1(2022), pp. 329-339
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2201329S
Original
scientific paper
ASSOCİATİON OF IL-1Β (-511 C / T) AND IL-1Β (-31 T / C) GENE POLYMORPHİSM WİTH ENDOPLASMİC RETİCULUM STRESS MARKER LEVELS İN ACUTE DECOMPENSATED HEART FAİLURE WİTH LOW EJECTİON FRACTİON
Ramazan SABIRLI1, Aylin KÖSELER2, Ergun METE3, Ibrahim TÜRKÇÜER4
1 Bakircay University Faculty of Medicine, Department of Emergency Medicine, Cigli Training and Research Hospital, İzmir, Turkey
2 Pamukkale University Faculty of Medicine, Department of Biophysics, Denizli, Turkey
3 Pamukkale University Faculty of Medicine, Department of Medical Microbiology, Denizli, Turkey
4 Pamukkale University Faculty of Medicine, Department of Emergency Medicine, Denizli, Turkey
Abstract
Sabırlı R., A. Köseler, E. Mete, I. Türkçüer (2022). Association of ıl-1β (-511 c / t) and ıl-1β (-31 t / c) gene polymorphism with endoplasmic reticulum stress marker levels in acute decompensated heart failure with low ejection fraction. - Genetika, Vol 54, No.1, 329-339.
Inflammatory processes play an important role in the pathogenesis of heart failure. The accumulation of unfolded proteins in the endoplasmic reticulum lumen and the unfolded protein response signal path is activated. The IL-1Β gene is located in the Chromosome 2.q14 region. -31 and -511 single nucleotide polymorphisms (SNPs) were detected in the IL-1Β promoter region. These two SNPs affect IL-1expression. This study aims to investigate the presence of IL-1Β (-511 C / T) and IL-1Β (-31 T / C) gene polymorphisms and the relationship between ER stress markers and inflammatory markers. Patients who applied to the department of emergency medicine with the findings of acute decompensated heart failure. Polymorphic sites of the IL-1Β gene were determined by DNA sequencing. In all study, individuals with IL-1Β (-31 T / C) T allele have higher serum PERK, GRP-78, CHOP and CRP levels median values than individuals with IL-1Β (-31 T/C) C allele (p = 0.0001, p = 0.002, p = 0.002 and p = 0.011, respectively). Serum ERK and GRP-78 values in HF group were higher in individuals with IL-1Β (-31 T / C) T allele compared to individuals with C allele (p = 0.0001 and p = 0.006). There was a statistically significant difference in serum CHOP levels in the control group with the IL-1Β (-511 C / T) T allele and the individuals with the C allele in the HF group (p = 0.002). In conclusion, we consider that the inflammatory response caused by IL-1Β (-31 T / C) gene polymorphism increased and the ER stress response increased, inflammatory pathway and ER stress of having IL-1Β (-31 T / C) T / T genotype or T allele.
Keywords: IL-1Β (-511 C / T), IL-1Β (-31 T / C), Gene Polymorphism, Endoplasmic Reticulum Stress, Heart Failure.
Corresponding author: Prof. Dr. Aylin Köseler, Pamukkale University Medical Faculty Department of Biophysics Denizli, Turkey,email: akoseler@pau.edu.tr
Published in „GENETIKA“ Vol. 54, No. 1(2022), pp 341-367
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2201341C
Original
scientific paper
GENOMIC CHARACTERIZATION AND PHYTOSTIMULATIVE EFFECT
OF A NOVEL SERRATIA SPECIES
Agit ÇETINKAYA1, Ömür BAYSAL1*, Ragıp Soner SILME2*, M. Kamran AZIM3,
Faizan SALEEM3
1Molecular Microbiology Unit in Department of Molecular Biology and Genetics, Faculty of Science, Muğla Sıtkı Koçman University, Menteşe-Muğla, 48121, Turkey
2Center for Research and Practice in Biotechnology and Genetic Engineering, Istanbul University, Istanbul, Turkey
3Department of Biosciences, Mohammad Ali Jinnah University, Karachi, Pakistan
Abstract
Çetinkaya A., Ö. Baysal, R. S. Silme, M. K. Azim, F. Saleem (2022). Genomic characterization and phytostimulative effect of a novel serratia species - Genetika, Vol 54, No.1, 341-367.
Some of non-pathogenic bacteria are effective biocontrol agents and plant growth inducers besides its degradative property on polycyclic aromatic hydrocarbons (PAH). Herein, we report a novel candidate Serratia species isolated in the purpose of PAH degradation, with its plant-growth-promoting and antifungal effect against Phytophthora infestans. Properties of bacterium determined by antifungal and phytostimulation assay under in vitro conditions displayed production of indole-3-acetic acid (IAA), chitinase and endoglucanase/cellulase activity. The identification of bacterium using whole-genome shotgun sequencing output also showed that the novel strain belongs to new Serratia species harboring the genes responsible for different secondary metabolites at the genomic level. Genome-wide analysis suggested a new candidate Serratia species (strain AGBY19) showing, in some extend, genetic relation with Serratia fonticola at molecular phylogeny level, which inhibits the growth of phytopathogenic fungi Phytophthora infestans by 73% compared to the control observed in vitro conditions. This strain colonised at the rhizosphere of tomato plant during in vivo host plant cultivation assay that remarkably enhanced the root growth. It causes the production of IAA hormone and cell wall degrading enzymes (chitinase, endoglucanase/cellulase). Further genome analyses of AGBY19 revealed different gene clusters comprising flanked regions associated with the production of secondary metabolites. These data eventually have provided its biocontrol properties and plant-growth inducer effect with globally potential to use for agricultural production.
Keywords: Biocontrol, Induced resistance, Phytophthora infestans, PGPR genes, Serratia, Whole genome sequencing
Corresponding author: Ömür Baysal, Molecular Microbiology Unit in Department of Molecular Biology and Genetics, Faculty of Science, Muğla Sıtkı Koçman University, Kötekli-Muğla, 48121, Turkey, E-mail: omurbaysal@mu.edu.tr
Published in „GENETIKA“ Vol. 54, No.1(2022), pp. 369-378
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-ZemunSerbia
UDC 575.630
https://doi.org/10.2298/GENSR2201369S
Original
scientific paper
SPECIES IDENTIFICATION AND GENETIC DIVERSITY OF Alcea (Malvaceae)
USING SCOT MOLECULAR MARKERS: MEDICINAL PLANT
Yanxia SUN1,2, Hanxiu JIANG1,2, Fang ZENG1,2, Xin PAN1,2, Xiaoxia WU1,2,
Yue QI1,2, Xiaoyong WU1,2*
1School of Food and Bioengineering, Chengdu University, Chengdu 610106, China
2Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affair,
Chengdu University Chengdu 610106, China
Abstract
Sun Y., H. Jiang, F. Zeng, X. Pan, X. Wu, Y. Qi, X. Wu (2022). Species identification and genetic diversity of Alcea (Malvaceae) using SCOT molecular markers: medicinal plant. - Genetika, Vol 54, No.1, 369-378.
The genus Alcea that is a member of Malvaceae family, is a Mediterranean perennial herb with major diversity centers in the Middle East and Western Mediterranean Basin. Alcea is a unique genus with several endemic taxa, and many of these endemic taxa from the Irano-Turanian phytogeographical area are particularly interesting. The genus contains 34 species in Iran, 15 of which are endemic. As a result of the importance of Alcea species, molecular data were collected for this genus in this research. For this study, 83 plants were used, which were randomly obtained from 5 species found in 6 provinces. Genomic DNA Amplification with 5 primers resulted in 75 bands, 66 of which were polymorphic (88.12%). The high average MI and PIC values indicated that SCoT primers have a high capacity for detecting polymorphic loci among the species of Alcea. The range of genetic similarities between 5 collected species was estimated to be between 0.77 and 0.89. The SCoT markers analysis revealed that the species Alcea angulata and Alcea popovii had the least similarity, while Alcea loftusii and Alcea sulphurea had the most similarity. The current study’s objectives are as follows: 1) is it possible to identify Alcea species through SCoT markers, 2) what genetic structure do these species have in Iran, and 3) what is the species inter-relationship? The current research found that SCoT markers can be used to identify the species.
Keywords: Alcea; Iran, Species Identification; Structure, SCoT (Start Codon Targeted)
Corresponding author: Xiaoyong Wu, School of Food and Bioengineering, Chengdu University, Chengdu 610106, China, Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affair, Chengdu University, Chengdu 610106, China E-mail: wuxiaoyong@cdu.edu.cn
Published in „GENETIKA“ Vol. 54, No. 1(2022), pp. 379-394
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.11
https://doi.org/10.2298/GENSR2201379F
Original
scientific paper
EVALUATION OF GRAIN YIELD OF WHEAT GENOTYPES USING STRESS TOLERANCE INDICES
Aref FATEHI1, Esmail BABARASHI1, Shahram MEHRI2*, Mehdi SALAJEGHEH3
1Department of Agronomy and Plant Breeding, Islamic Azad University, Sanandaj Branch, Iran
2Department of Agronomy and Plant Breeding, Pars Abad Moghan Branch, Islamic Azad University, Pars Abad Moghan, Iran
3Department of Agronomy, Islamic Azad University, Bam Branch, Iran
Abstract
Fatehi A., E. Babarashi, S. Mehri, M. Salajegheh (2022). Evaluation of grain yield of wheat genotypes using stress tolerance indices - Genetika, Vol 54, No.1, 379-394.
Drought
has unfavorable impacts on the success of agriculture in many parts of the
world.
Providing future populations with enough food would obviously require the
evaluation of crop yield, higher potentials, and the provision of yield
stability in drought-affected regions. In this research, the drought-tolerance
of wheat genotypes was studied in a randomized complete block design and in a
three-replication experiment under normal and drought stress conditions. In two
consecutive growing seasons, the measurements were aimed at evaluating the
stress susceptibility index (SSI), drought tolerance index (TOL), mean
productivity (MP, stress tolerance index (STI, harmonic mean (HARM), yield
index (YI, and genomic mean productivity
(GMP).
These parameters described the yields of different genotypes for two years and
under both normal and stress conditions. The combined analysis of variance
showed that the environment significantly affected grain yield. The mean values
of parameters by the drought stress condition were less than those of the
non-stress condition. Stress intensity
(SI) was 46% and 43% in the first and second year, respectively. In both years, MP,
GMP, STI and HARM indices correlated significantly with grain yield under
stress and normal conditions. Based on a three-dimensional diagram of these
indices, the pishgam and ws-82-9 genotypes were considered as most superior in
the first year (both conditions).. Furthermore, the pishgam, alvand, and ohadi
genotypes were considered as superior in the second year. According to the
bi-plot diagram and based on the first two major components, these genotypes
were more tolerant to drought stress. In general, it is suggested that the
pishgam shows a higher level of yield sustainability. It was found to be the
genotype with the highest yield under both normal and stress conditions. Its
grain yield and resistance indices have increased during 80 years of breeding
and selection.
Keywords: Biplot,Grain yield, Drought tolerance, Indices, Genotype
Corresponding author: Shahram Mehri, Department of Agronomy and Plant Breeding, Pars Abad Moghan Branch, Islamic Azad University, Pars Abad Moghan, Iran. E-mail: sh.mehri2000@gmail.com & sh.mehri@iaupmogan.ac.ir
Published in „GENETIKA“ Vol. 54, No.1(2022), pp. 395-409
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2201395B
Original scientific paper
CLINICAL EXOME SEQUENCING IN SERBIAN PATIENTS WITH MOVEMENT DISORDERS - SINGLE CENTRE EXPERIENCE
Marija BRANKOVIĆ,1,2, Nataša DRAGAŠEVIĆ1,2, Valerija DOBRIČIĆ1,3, Aleš MAVER4, Gaber BERGANT4, Igor PETROVIĆ1,2, Stojan PERIĆ1,2, Ana MARJANOVIĆ1,2, Milena JANKOVIĆ1, Jasna JANČIĆ2,5, Ivana NOVAKOVIĆ2, Borut PETERLIN4, Marina SVETEL1,2, Vladimir KOSTIĆ1,2
1Neurology Clinic, University Clinical Center of Serbia, Belgrade, Serbia
2Faculty of Medicine, University of Belgrade, Belgrade, Serbia
3Luebeck Interdisciplinary Platform for Genome Analytics (LIGA), University of Luebeck, Luebeck, Germany
4Clinical Institute of Medical Genetics, University Medical Centre Ljubljana, Ljubljana, Slovenia
5Clinic of Neurology and Psychiatry of Children and Youth, University of Belgrade, Belgrade, Serbia
Abstract
Branković M., N. Dragašević, V. Dobričić, A. Maver, G. Bergant, I. Petrović, S. Perić, A. Marjanović, M.Janković, J. Jančić, I. Novaković, B. Peterlin, M. Svetel, V. Kostić (2022). Clinical exome sequencing in Serbian patients with movement disorders - single centre experience. - Genetika, Vol 54, No.1, 395-409.
The aim of the study was to analyze the genetic basis of a various range of neurodegenerative disorders manifesting by movement disorders (MD) using next generation sequencing (NGS) clinical exome panel. The study included a total number of 42 cases, 36 unrelated and 3 sibling pairs patients diagnosed with movement disorders, all negative after targeted genetic testing available at Neurology clinic, UCCS, Belgrade, Serbia. In a selection of respondents, preference was given to family cases with the early presentation, patients with a positive family history, or complex MD phenotype. Sequencing of a Clinical exome (CE) panel for 4813 genes with known associated clinical phenotypes was performed on an Illumina MiSeq NGS platform according to the manufacturer’s instructions. Sequence variants were analyzed by Illumina’s Variant Studio v3 software as well as using previously developed pipeline. Variants analysis and interpretation were based on phenotype gene target approach, literature and databases search, allele frequency, and pathogenicity prediction by in silico software. Causative variants were confirmed by Sanger sequencing. Whenever possible, additional family members were studied for segregation analysis. We identified a likely genetic cause of MD in 5 cases. CE panel analysis revealed 7 different missense and one splice site pathogenic/likely pathogenic variants in 5 genes related to rare neurodegenerative disorders. Detected pathogenic/likely pathogenic variants in the TUBB4A, PANK2, SETX, MFSD8, and ARSA genes have been compatible with the clinical phenotype of the patients. Furthermore, in additional three cases variants in the DCTN1, PDGFRB, and POLG genes have been detected as a possible cause of disease. In the rest of the studied cases, genetic diagnosis remains unclear. These results emphasize the significance of CE panel analysis in elucidating the diagnosis of neurodegenerative diseases manifesting by movement disorders and gave us insight into the complexity of the genetic background of this group of disorders.
Keywords: clinical exome sequencing, movement disorders, DNA diagnostics, genetics, gene variant
Corresponding author: Marija Branković, MSc, dr Subotića starijeg 6, 11000 Belgrade, Serbia. Phone:+381112658355; E-mail: mara.brankovic@gmail.com
Published in „GENETIKA“ Vol. 54 No.1(2022), pp. 411-426
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2201411K
Original
scientific paper
MOLECULAR CHARACTERIZATION OF METHICILLIN-RESISTANT Staphylococcus aureus (MRSA) ISOLATED FROM BOVINE WOUNDS
Muhammad Usman Faryad KHAN1, Asif NADEEM1,2, Maryam JAVED1, Wasim SHEHZAD1, Muhammad Asad ALI1
1University of Veterinary and Animal Sciences, Lahore - Pakistan
2Department of Biotechnology, Virtual University of Pakistan, Lahore - Pakistan
Abstract
Khan M. U. F., A. Nadeem, M. Javed, W.Shehzad, M. A. Ali (2022). Molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) isolated from bovine wounds. - Genetika, Vol 54, No.1, 411-426.
Staphylococcus aureus (S. aureus) is a Gram-positive coccus with golden-colored colonies 0.5-1.5 µm in diameter. It is an opportunistic pathogen and colonizes as healthy flora. When the host defense system is breached it provides a source for the introduction of (Methicillin-resistant S. aureus) MRSA. The incorporation of the mecA gene shift S. aureus into MRSA, mecA is a primary gene for the confirmation of MRSA, so, it is used as a useful marker to determine Methicillin resistance in S. aureus. In this study, we investigated the molecular characterization of mecA, Panton-Valentine Leukocidin (PVL), and 16S rRNA genes in MRSA to determine diversity, phylogenetic analysis, and multidrug resistance (MDR) of MRSA isolated from chronic bovine wounds. A total of 8 antibiotics were used for MDR profiling and the results obtained are as follows: 100% of MRSA isolates were resistant to Augmentin and Cefipime, 81.8% to Vancomycin and Tetracyclin, 36.4% to Streptomycin and Ciprofloxacin, Azithromycin 54.5 and 0% to Chloramphenicol which warrants that it’s the best antibiotic according to this study. Multiplex PCRs were performed for the confirmation of targeted genes and diversity analysis. The diversity of MRSA in the bovine population was 22% (11/50) on the microbiological scale that considered high as compared to reported data. When the PCR of MRSA isolates was performed, there was a unique phenomenon observed i.e., no mecA gene was present in 2 isolates 18.18% (2/11) which connotes the importance of molecular methods/PCR for the identification of microbes. The prevalence of the PVL gene was 18.18%, comparatively high as compared to previous studies conducted on bovine chronic wounds. When the Sanger sequencing of 16S rRNA of MRSA isolates was performed there was a change of one nucleotide identified (C>T) at position 1031. After performing phylogenetic analysis with S. aureus of different countries distinct and separate dendrogram was obtained which differentiates the Pakistani S. aureus isolates from other countries.
Keywords: Bovine, MRSA, Staphylococcus aureus, Phylogenetic Analysis, Multiplex PCR.
Corresponding author: Asif Nadeem, University of Veterinary and Animal Sciences, Lahore - Pakistan asif.nadeem@vu.edu.pk
Published in „GENETIKA“ Vol. 54, No.1(2022), pp. 427-438
© 2022Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2201427M
Original scientific paper
COMPARISON OF ABBOTT REAL TIME SARS-COV-2 ASSAY, GENEFINDER™ COVID-19 PLUS REALAMP KIT AND BIOMERIEUX ARGENE® SARS-COV-2 R-GENE® KIT FOR THE RT-PCR BASED DETECTION OF SARS-COV-2 FROM NASOPHARYNGEAL SWABS
Jelena STOJČEVIĆ MALETIĆ1,2, Iva BARJAKTAROVIĆ2,3, Vanja RADENKOVIĆ2,
Velibor ČABARKAPA²,4
1Department of Biochemistry, Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia
2Center for Laboratory Medicine, Clinical Center of Vojvodina, Novi Sad, Serbia
3Department of General Education Subjects, Faculty of Medicine, University of Novi Sad,
Novi Sad, Serbia
4Department of Pathological Physiology and Laboratory Medicine, Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia
Abstract
Maletić Stojčević J., I. Barjaktarović, V. Radenković, V. Čabarkapa (2022). Comparison of abbott Real Time SARS-COV-2 ASSAY, Genefinder™ Covid-19 plus realamp kit and Biomerieux Argene® SARS-COV-2 R-Gene® kit for the RT-PCR based detection of SARS-COV-2 from nasopharyngeal swabs. - Genetika, Vol 54, No.1, 427-438.
Early, rapid and reliable identification of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) is crucial for successful control of coronavirus disease 2019 (COVID-19). The quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) assay is considered the gold standard for molecular diagnosis of SARS-CoV-2. The objective of this study was to compare the clinical performances of the three authorized tests — the Abbott Real Time SARS-CoV-2 (ACOV) assay (Abbott Molecular Inc., North Chicago, IL), GeneFinder™ COVID-19 Plus RealAmp (GeneFinder) Kit (OSANG Healthcare Co., Ltd, Dongan-gu Anyang, Korea) and the Biomerieux ARGENE® SARS-COV-2 R-GENE® real-time detection (ARGENE) kit (bioMérieux SA., Marcyl’Étoile, France) and to determine whether the selection of targeted genes has an impact on test's specificity. In this study, we included 155 nasopharyngeal swabs (NPS) from adult individuals with symptoms or suspected of COVID-19, aged from 17 to 91 years, previously tested by the ACOV and subsequently tested by the GeneFinder and the ARGENE. In this comparative analysis, we found that the GeneFinder assay detected the most cases of COVID-19 infection, followed by the ACOV assay, and then by ARGENE. Positive agreement ranged from 74.74% to 95.41%, with the strongest agreement observed between the GeneFinder and ACOV assays — 95.41% (95% confidence intervals (CI): 89.37%-98.36%) indicating an excellent agreement between these two tests and the lowest agreement between the GeneFinder and ARGENE assay — 74.74% (95% CI: 65.08%-81.41%). The negative percent agreement was 100% (GeneFinder/ACOV, GeneFinder/ARGENE and ACOV/ARGENE). Only 3.2% of cases were false-negative using the ACOV test, while 18.0% of samples were false-negative using the ARGENE assay to detect SARS-CoV-2. Combined usage of the Abbott SARS-CoV-2 and the GeneFinder assays can be applied to maximize SARS-CoV-2 detection accuracy.
Keywords: COVID-19, SARS-CoV-2; molecular testing, RT-PCR, in vitro diagnostic tests
Corresponding author: Jelena Stojčević-Maletić, Department of Biochemistry, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia. E-mail: jelena.stojcevic-maletic@mf.uns.ac.rs
Published in „GENETIKA“Vol. 54, No.1(2022), pp. 439-446
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2201439B
Original
scientific paper
RELATIONSHIP BETWEEN PEDIATRIC CELIAC DISEASE AND CHROMATIN REMODELING GENE EXPRESSIONS
Seda Orenay BOYACIOGLU1*, Metin CALISKAN2, Guzide DOGAN3
1Department of Medical Genetics, Faculty of Medicine, Aydin Adnan Menderes University, Aydin, Turkey
2Department of Medical Biology, Faculty of Medicine, Usak University, Usak , Turkey
3Department of Pediatric Gastroenterology, Faculty of Medicine, Bezmialem University, Istanbul, Turkey
Abstract
Boyacioglu Orenay S., M. Caliskan, G. Dogan (2022). Relationship between pediatric celiac disease and chromatin remodeling gene expressions - Genetika, Vol 54, No.1, 439-446.
Celiac disease (CD) is an immune-dependent systemic disorder that occurs in genetically predisposed individuals resulting in damage in the small intestine. It is known that chromatin remodeling, an epigenetic mechanism, is associated with gastrointestinal diseases associated with chronic inflammation. However, no information is available on the link between CD and chromatin remodeling. For this purpose, the expression profiles of chromatin remodeling group genes in children diagnosed with CD according to Marsh classification and HLA profile were evaluated and their relationship with CD was investigated. Endoscopic biopsies embedded in the paraffin block of 40 children with CD diagnosis and 30 healthy children were included in the study. The most common four mutations (DQA1*05, DQB1*02, DQA1*03, and DQB1*03:02) related to CD on human leukocyte antigen (HLA) gene were screened. Intestinal biopsy samples were used for mRNA isolations and cDNA synthesis. Expressions of total seven genes in the chromatin remodeling groups (SWI/SNF Complex Group: ARID1A, Polycomb Group: CTBP1, Nucleosome-Remodeling & Histone Deacetylase (NuRD) Complex Group: MTA1, Chromobox/Heterochromatin Protein 1 (HP1) Homologs Group: CBX3 and CBX7, Homeodomain (PHD) Protein Group: NSD1, Inhibitor of Growth (ING) family group: ING 5) were analyzed by Real-Time qPCR. Data analysis was performed online using the software provided by the manufacturer. Overexpression in ARID1A, CTBP1, and NSD1 genes was detected when the CD group was compared against the control group, however they were not significant (p=0.31, 0.33, and 0.33). When CD group who had diarrhea symptom (typical) were compared to the CD group without diarrhea symptom (atypical), statistically significant under-expression was found in CBX3 and CTBP1 genes (p=0.04 and p=0.004). Statistically significant CTB1 overexpression was detected in Marsh 2 CD cases (p=0.03). In the comparison of HLA DQ2/DQ8 positive CD patient group with the control group, the NSD1, CBX3, and EED (p=0.75, 0.75, and 0.78) genes were over-expressed and the CBX7, MTA1, ARID1A, and CTBP1 genes (p=0.74, 0.75, 0.75, and 0.75) were under-expressed. This is the first study to report that expression of chromatin remodeling genes may have roles in the development and progression of CD. The results of this case-control study are open to confirmation by future studies with larger number of subjects to obtain statistically significant results.
Keywords: Pediatric celiac disease, chromatin remodeling, gene expression, chronic inflammation, autoimmunity
Corresponding author: Seda Orenay-Boyacioglu, Department of Medical Genetics, Faculty of Medicine, Aydin Adnan Menderes University, Aydin, Turkey, Tel: +90 539 277 7679; Fax: +90 256 2146495; E-mail: sorenay@adu.edu.tr
Published in „GENETIKA“Vol. 54, No1(2022), pp. 447-456
© 2022 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2201447D
Original scientific paper
PREVALENCE OF VARIANTS IN DFNB1 LOCUS IN SERBIAN PATIENTS WITH AUTOSOMAL RECESSIVE NON-SYNDROMIC HEARING LOSS
Bojana DOBRIC1, Danijela RADIVOJEVIC1, Jovana JECMENICA2, Pavlos FANIS3,
Vassos NEOCLEOUS3, Leonidas A PHYLACTOU3, Marina DJURISIC1
1Laboratory of Medical Genetics, Mother and Child Health Care Institute of Serbia
“Dr Vukan Cupic”, Belgrade, Serbia
2Department of Otorhinolaryngology, Mother and Child Health Care Institute of Serbia
’’Dr Vukan Cupic“, Belgrade, Serbia
3Department of Molecular Genetics, Function and Therapy, The Cyprus Institute of Neurology and Genetics, Nicosia, Cyprus
Abstract
Dobric B., D. Radivojevic, J. Jecmenic, P. Fanis, V. Neocleous, L.A Phylactou, Marina Djurisic (2022). Prevalence of variants in DFNB1 locus in Serbian patients with autosomal recessive non-syndromic hearing loss. - Genetika, Vol 54, No.1, 447-456.
Hearing impairment is the most common sensorineural disorder in humans and many genes have been identified as causable. Despite genetic heterogeneity, a single locus, DFNB1, that contains genes GJB2 and GJB6, accounts for up to 50% of all cases. Aim of this study was to determine prevalence of identified variants in DFNB1 locus in patients from Serbia with autosomal recessive non-syndromic hearing loss (ARNSHL). In this study, PCR-ARMS and direct sequencing of the GJB2 and GJB6 genes was carried out in 54 probands and relatives from Serbia with nonsyndromic hearing loss (NSHL). In 31 patients a series of variants have been identified in the GJB2 gene. Fully characterized genotype with bi-allelic mutations was observed in 40.74% of the probands (22/54). The remaining probands were either identified in the heterozygote form (9/54) or were identified with no (23/54) causing variants for the tested genes. A total of seven different mutations were found with following allele frequencies: c.35delG (31.48%), c.71G>A (6.48%), c.313_326del (5.56%), c.101T>C (1.85%), c.380G>A (1.85%), c.79G>A (0.92%) and c.269T>C (0.92%). The molecular basis of NSHL in patients from Serbia was analyzed for the first time in this study. The results have important implication to the development of the genetic diagnosis of deafness, genetic counseling, and early treatment in our country. Also, our findings contribute to the knowledge of geographic distribution of DFNB1 mutations.
Keywords: autosomal recessive non-syndromic hearing loss, GJB2 gene, GJB6 gene, variants
Corresponding author: Bojana Dobric, Laboratory of Medical Genetics Mother and Child Health Care Institute of Serbia “Dr Vukan Cupic”, Radoja Dakica str.6-8, 11070 Belgrade, Serbia, Tel: ++381 11 3108 273, E-mail: bojana_dobric@yahoo.com
Published in „GENETIKA“Vol. 54, No.1(2022), pp.457-472
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2201457P
Original scientific paper
IMPACT OF THE FETUIN GENE POLYMORPHISMS IN CORONARY ARTERY CALCIFICATION AND MORTALITY OF PATIENTS WITH CHRONIC KIDNEY DISEASE AND RENAL TRANSPLANT
Svetlana JOVIČIĆ PAVLOVIĆ1*, Sanja SIMIĆ OGRIZOVIĆ2,3, Zoran BUKUMIRIĆ4
Milena ERIĆ5, Natalija PAVLOVIĆ6, Boba Kotlica7, Ivana NOVAKOVIĆ8
1Clinic for Nephrology, University Clinical Centre of Serbia, Belgrade, Serbia
2General hospital Medigroup, Belgrade, Serbia
3Medical School, University in Banja Luka, Banja Luka, Republic of Srpska
4Institute for medicine statistics, Medical School, University in Belgrade, Serbia
5Institute for virusology, vaccines and serums, Torlak, Belgrade, Serbia
6Covid Hospital, University Clinical Centre of Serbia, Belgrade, Serbia
7Medical School, University in Belgrade, Serbia
8Institute for human genetics, Medical School, University in Belgrade, Serbia
Abstract
Pavlović Jovičić S., S. Simić Ogrizović, Z. Bukumirić, M. Erić, N. Pavlović, B. Kotlica, I. Novaković (2022). Impact of the fetuin gene polymorphisms in coronary artery calcification and mortality of patients with chronic kidney disease and renal transplant. - Genetika, Vol 54, No.1, 457-472.
Fetuin A is a major systemic inhibitor of vascular calcifications. The aim of this study was to examine association of single nucleotide polymorphisms (SNP) in the gene for fetuin-A with fetuin-A serum levels, coronary arteries calcification (CAC) and mortality in renal transplant (RT) and chronic kidney (CKD) patients. This study included 88 patients (42 stable RT patients at least 6 months after transplantation and 46 CKD patients, stage 2-5 not requiring dialysis) followed five years. Detection and analysis of fetuin A gene polymorphisms in positions C742T (Thr248Met; rs4917) and C766G (Thr256Ser; rs4918) were performed using PCR method. Respondents with allele 742T had at the same time 766G. Combined genotypes TT/GG had lower serum fetuin A levels than CT /CG and CC/CC. Predictors of CAC in univariate analysis were age (p=0,000), serum fetuin-A levels (p=0.011) and rs 4917 polymorphism (p=0.021) while multivariate determined age (p=0.001) and fetuin-A levels (p=0.031). Patients who were homozygous for variant 742T and 766G (combined genotype TT/GG) had lowest survival rate. Our results suggest that allele 742T and 766G in gene for fetuin-A were associated with lower serum fetuin-A levels, higher CAC occurrence and higher mortality rate in RT and CKD patients.
Keywords: fetuin-A, gene polymorphism, vascular calcifications, chronic kidney disease, renal transplantation
Corresponding author: Svetlana Jovicic Pavlovic, Clinic for Nephrology, University Clinical Centre of Serbia, Belgrade, Serbia. E-mail: svetlanvicic.pavlovic@gmail.com
Published in „GENETIKA“Vol. 54, No.1(2022), pp.473-489
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.
https://doi.org/10.2298/GENSR2201473K
Original scientific paper
SUNFLOWER AGRONOMIC TRAITS IN FIELD IRRIGATION CONDITIONS
Antonela MARKULJ KULUNDŽIĆ1*, Aleksandra SUDARIĆ1, 2, Maja MATOŠA KOČAR1*, Anto MIJIĆ1, Ivica LIOVIĆ1, Marija VILJEVAC VULETIĆ1, Ivana VARGA3, Vera CESAR4,6, Hrvoje LEPEDUŠ5,6
1Agricultural Institute Osijek, Osijek, Croatia
2Centre of Excellence for Biodiversity and Molecular Plant Breeding, University of Zagreb, Faculty of Agriculture, Zagreb, Croatia
3J.J. Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, Osijek, Croatia
4J.J. Strossmayer University of Osijek, Department of Biology, Osijek, Croatia
5J.J. Strossmayer University of Osijek, Faculty of Humanities and Social Sciences, Osijek, Croatia
6J.J. Strossmayer University of Osijek, Faculty of dental medicine and health, Osijek, Croatia
Abstract
Kulundžić Markulj A., A. Sudarić, M. Matoša Kočar, A. Mijić, I. Liović, M. Viljevac Vuletić, I. Varga, V. Cesar, H. Lepeduš (2022). Sunflower agronomic traits in field irrigation conditions- Genetika, Vol 54, No.1, 473-489.
Multi-year studies are crucial for the evaluation of sunflower hybrids and quantifying the environmental effect in the expression of genetic potential. In order to assess the adaptation of eight hybrids and test the impact of water availability on plant height (PH), head diameter (HD), 1000-grain weight (TWG), hectolitre mass (HM), seed yield (SY), oil content (OC) and oil yield (OY), a two-year study was conducted with irrigation as treatment. Analysis of variance (ANOVA) and principal component analysis (PCA) confirmed the year influence on all tested agronomic traits. According to the three-way ANOVA, irrigation significantly affected all tested traits except HD, OC and OY. All agronomic traits had lower values in both rainfed and irrigated treatment in 2014, confirming the influence of the environment. The hybrid was a significant source of variation for all traits. ANOVA and PCA grouped hybrids 1, 7, and 8 in one group and 2, 3, 4, 5, and 6 in another. The first group had lower PH, HD, TGW and SY values and higher HM, OC and OY values, and the second group had reversed traits values. Furthermore, the PCA biplot indicates SY was positively correlated with PH, HD, TGW and OY and HM was positively correlated with OC. This facilitates the breeding process because it enables indirect breeding for economically important traits such as seed yield, oil content and oil yield. As treatments were significant sources of variation for PH, HM, TWG and SY, sunflower irrigating is considered justified and can be used as an additional agrotechnical measure to target the agronomic traits. Understanding the expression of traits under rainfed and irrigation conditions will greatly help design effective breeding programs by creating hybrids suitable for cultivation in semi-arid environments.
Keywords: Helianthus annuus L., Environment, Water availability, Agronomic properties, Yield
Corresponding author: Antonela Markulj Kulundžić, Maja Matoša Kočar, Agricultural Institute Osijek, Južno predgrađe 17, 31000, Osijek, Croatia, E-mail: antonela.markulj@poljinos.hr; maja.matosa@poljinos.hr
Published in „GENETIKA“Vol. 54, No.1(2022), pp.491-498
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR22010491J
Original scientific paper
Katarina JOVANOVIC1, Jelena CARKIC2, Nadja NIKOLIC2, Nada NOVAKOVIC1, Milos HADZI-MIHAILOVIC1, Emina COLAK3, Sasa CAKIC1, Jelena MILASIN2
1University of Belgrade, School of Dental Medicine, Department of Periodontology and Oral Medicine, Dr Subotica 8, Belgrade, Serbia.
2University of Belgrade, School of Dental Medicine, Department of Human Genetics, Dr Subotica 8, Belgrade, Serbia.
3Institute of Medical Biochemistry, Clinical Centar of Serbia, Visegradska 26, Belgrade, Serbia.
Abstract
Jovanovic K., J. Carkic, N. Nikolic, N. Novakovic, M. Hadzi-Mihailovic, E. Colak, S. Cakic, J. Milasin (2022). Detection and quantification of pathogenic microorganisms in patients with chronic periodontitis before and after treatment with chlorhexidine digluconate. - Genetika, Vol 54, No.1, 491-498.
Keywords: Chronic periodontitis, Periopathogenic microorganisms, PCR, qPCR, Chlorhexidine digluconate
Corresponding author: Jelena Milasin, University of Belgrade, School of Dental Medicine, Department of Human Genetics, Dr Subotica 8, 11000 Belgrade, Serbia. Phone: (+381)63 7596550, e-mail: jelena.milasin@stomf.bg.ac.rs
Published in „GENETIKA“Vol. 54, No.1(2022), pp.499-512
© 2022 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC
575.
https://doi.org/10.2298/GENSR2201499B
Review paper
AN OVERVIEW OF RUST (Uromyces viciae-fabae) AND POWDERY MILDEW
(Erysiphe polygoni DC) OF PEA (Pisum sativum L.)
Deepa BENIWAL1*, R.K. DHALL1, Saurabh YADAV1, Priti SHARMA2
1Department of Vegetable Science, Punjab Agricultural University, Ludhiana-141004, India
2School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana-141004, India
Abstract
Beniwal D., R.K. Dhall, S. Yadav, P. Sharma (2022). An overview of rust (Uromyces viciae-fabae) and powdery mildew (Erysiphe polygoni DC) of pea (Pisum sativum L.- Genetika, Vol 54, No.1, 499-512.
Pea is a self-pollinating, cool season leguminous crop with a diploid chromosome number of 14. Pea is cultivated extensively and because of high protein content, pea is a crop with great significance. However, cultivation of pea gets affected by numerous biotic and abiotic stresses. Fungal diseases such as rust, powdery mildew, fusarium wilt etc. comes under the biotic stresses which are most widespread. Rust and powdery mildew cause major damage to the crop in both tropical and temperate locales of the world. Use of fungicide to control plant diseases is a good approach but excessive use of fungicide can cause environmental pollution and disasters throughout the world and can also built resistance in the pathogens. Therefore, to remove these constraints, disease resistant varieties must be used. Use of resistant varieties is a safe and efficient alternative method to control plant diseases. Breeding for rust and powdery mildew resistance has been started globally and a number of resistant sources have been identified. To introgress resistant gene into commercial varieties of pea, molecular tools must be integrated with conventional breeding techniques. Till date only one linkage map has been generated for rust resistance in pea; while for powdery mildew, three genes have been mapped. Molecular markers linked to these genes can be used in breeding programs of resistance varieties. To improve the efficiency of selection for rust and powdery mildew resistance and enhance varietal development, the integrated approach of genomic resources, effective molecular tools and high resolution phenotyping tools must be used. An overview of pea rust and powdery mildew, pathogen structure, yield losses and breeding techniques implied to control these diseases, is provided in this review article.
Keywords: pea, rust, powdery mildew, molecular markers, resistant genes
Corresponding author: Deepa Beniwal, Department of Vegetable Science, Punjab Agricultural University, Ludhiana-141004, India, E-mail: deepabeniwal24@gmail.com
Published in „GENETIKA“Vol. 54, No.1(2022), pp513
© 2022Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
https://doi.org/10.2298/GENSR2201513E
CORRIGENDUM
by
Snežana
Mladenović Drinić, Editor of the journal Genetika
request
from Xiaohui Qian to remove name from the paper
DETECTING DNA POLYMORPHISM AND GENETIC DIVERSITY
IN A WIDE PISTACHIO GERMPLASM BY RAPD MARKERS
Xiaohui QIAN1* and Shahram MEHRI2
1Department of Tourism and Culture, Anhui Finance and Trade Vocational College,
Hefei, China
2Department of Agronomy and Plant Breeding, ParsAbad Moghan Branch, Islamic Azad University, ParsAbad Moghan, Iran
Original scientific paper
https://doi.org/10.2298/GENSR2102783Q
published in the journal Genetika, 2021, Vol 53, No.2, 783 -798