GENETIKA, Vol

GENETIKA, Vol. 55, No.2 (2023)

Viliana VASILEVA, Georgi GEORGIEV, Vera POPOVIЖ

GENOTYPIC SPECIFICITY OF SOYBEAN [Glycine max (L.) MERR.] PLASTID PIGMENTS CONTENT UNDER SOWING DATE AND INTERROW SPACING [Abstract] [Full text]

Maryam Jamshidnia, Sedigheh ASGARY, Mahmoud RAFIEIAN-KOPAEI

EVALUATION OF ACCESSION STRUCTURE AND GENETIC DIVERSITY IN IRANIAN MILK THISTLE (Silybum marianum L.) BY ISSR MARKERS [Abstract] [Full text]

Basalapura Rangegowda CHANDANA, Sampangi RAMESH, Rotti KIRANKUMAR, Gonal BASANAGOUDA and Mugali Pundalik KALPANA

ISOLATION AND BIOINFORMATICS CHARACTERIZATION OF HORSE GRAM (Macrotyloma uniflorum (Lam.) Verdc.) HOMOLOGS (MuTFL1 and MuLFY) OF GENES CONTROLLING GROWTH HABIT [Abstract] [Full text]

Jovan PAVLOV, Nenad DELIЖ, Zoran ИAMDЋIJA, Gordana BRANKOVIЖ, Nataљa MILOSAVLJEVIЖ, Nikola GRИIЖ, Sofija BOЋINOVIЖ

GRAIN YIELD STABILITY ESTIMATES OF LATE MATURITY MAIZE HYBRIDS [Abstract] [Full text]

Mahsa KAVOUSI and Afsaneh DELFANI

Solanum pseudo-capsicum EFFECTS ON Bax AND Bcl-2 GENE EXPRESSION AND APOPTOSIS IN MCF-7 CELL LINE [Abstract] [Full text]

Adikant PRADHAN, A. SAO, S. K. NAG, Nilamani DIKSHIT
CONSERVATION PRACTICES AND ASSESSMENT OF SPATIAL AND TEMPORAL VARIABILITY IN RICE LANDRACES OF BASTAR REGION OF CHHATTISGARH, INDIA [Abstract] [Full text]

Deniz ЄUMNULU
CONTRARY TO KNOWN SILIBININ IS NOT AN ANTICANCER AGENT AND LIVER PROTECTIVE SUPPLEMENT [Abstract] [Full text]

Zine El Abidine FELLAHI, Abderrahmane HANNACHI, Susanne DREISIGACKER, Deepmala SEHGAL, Amor YAHYAOUI, Hamenna BOUZERZOUR

GENETIC EFFECTS OF Rht-B1b AND Rht-D1b DWARFING ALLELES ON PLANT HEIGHT AND AGRONOMIC TRAITS OF BREAD WHEAT (Triticum aestivum L.) BREEDING LINES [Abstract] [Full text]

Yong WANG, Yuling L.LI, Feng SUN, Guohong H. WU, Aizezi Z. SUlAIMAN, Shijian J. BAI, Ronghua H. ZHAO, Pingfeng F. GUO

F₁ PROGENY OF ‘RUBY SEEDLESS’Ч ‘HONGQITEZAO’ DEVELOPED AND SELECTED USING EMBRYO-RESCUE AND MOLECLAR MARKERS [Abstract] [Full text]

Aydın UZUN, Emine DIRIM, Mehmet YAMAN

MORPHOLOGICAL AND MOLECULAR IDENTIFICATION OF HYBRID APRICOT GENOTYPES OBTAINED BY INTRA-SPECIFIC HYBRIDIZATION [Abstract] [Full text]

Olivera DJORDJEVIЖ MELNIK, Sofija BOЋINOVIЖ, Radmila VUKADINOVIЖ, Marija MILIVOJEVIЖ, Tanja PETROVIЖ, Tomislav ЋIVANOVIЖ, Jelena VANИETOVIЖ

SEED QUALITY OF RECIPROCAL MAIZE SINGLE CROSSES AND THEIR PARENTAL INBRED LINES [Abstract] [Full text]

Amit T. ADSUL, Vivek P. CHIMOTE, Dilip S. THAKARE, Milind P. DESHMUKH

MOLECULAR CHARACTERIZATION OF SEED LONGEVITY IN SOYBEAN [Glycine max (L.) Merrill] USING SSR MARKERS [Abstract] [Full text]

Ayesha SADDIQA, Asif NADEEM, Maryam JAVED, Tahir YAQUB, Nadia MUKHTAR, Jahanzaib AZHAR, Muhammad Salman TARIQ

INVESTIGATION OF THE CC CHEMOKINE RECEPTOR 5 (CCR5) DELTA32 AND GENE VARIANTS IN HIV INFECTED PATIENTS [Abstract] [Full text]

Sena ARDICLIand Fazli ALPAY

THE EFFECTS OF MC4R AND CACNA2D1 GENE POLYMORPHISMS ON CARCASS TRAITS AND MARBLING SCORE IN TURKISH NATIVE CATTLE BREEDS AND THEIR CROSSBREDS WITH THE HOLSTEIN-FRIESIANS [Abstract] [Full text]

Larisa Nikolayevna SKORYKH, Nadezhda Sergeevna SAFONOVA, Arslan Akhmetovich OMAROV, Nina Ivanovna EFIMOVA, Konstantin Alexsandrovich KATKOV, Violeta Caro PETROVIC, Natalia Igorevna KIZILOVA

ASSOCIATION OF SINGLE NUCLEOTIDE POLYMORPHISMS OF GH, LEP, MSTN GENES WITH GROWTH CHARACTERISTICS IN MEAT-WOOL AND WOOL SHEEP [Abstract] [Full text]

Bita ALIASGHAR, Saba SANE, Masoud SHEIDAI, Fahime KOOHDAR, Naser KALHOR

ASSOCIATION STUDY OF RS323344 IN TEX15 WITH NON-OBSTRUCTIVE AZOOSPERMIA IN IRANIAN POPULATION [Abstract] [Full text]

Zeynep DEMİRTAЄ ЄAHİN and Levent MERCAN

ANALYSIS OF GENETIC VARIATIONS IN TURKISH DOMESTIC GOOSE POPULATIONS AND CHINESE Ч EMBDEN CROSSES USING MICROSATELLITE MARKERS [Abstract] [Full text]

Gozde CEYLAN, Sefika Nur GUMUS, Sule SECKIN, Oner SANLI, Selcuk ERDEM, Canan KUCUKGERGIN

GENETIC VARIANTS OF ENDOTHELIAL NITRIC OXIDE SYNTHASE (eNOS4a/b) AND SUSCEPTIBILITY TO RENAL CELL CARCINOMA IN A TURKISH POPULATION [Abstract] [Full text]

Marija VUKOVIC,Milica MIHAJLOVIC SREJIC, Katarina SAPIC, Vanja TANASIC, Verica RADOJICIC, Milica KECKAREVIC MARKOVIC, Miljana KECMANOVIC, Dusan KECKAREVIC

EVALUATION OF THE IRISPLEX SYSTEM FOR EYE COLOUR PREDICTION IN THE SERBIAN POPULATION [Abstract] [Full text]

S. D.AEER, S. K. JINDAL, S. A. H. PATEL, N. CHAWLA

ASSESSMENT OF COMBINING ABILITY COMPRISING alc, nor AND rin MUTANT ALLELES OF TOMATO UNDER MAIN AND LATE PLANTING SEASONS [Abstract] [Full text]

Asuman KAPLAN EVLİCE, Єule KESKİN, Aliye PEHLİVAN, Turgay ЄANAL, Ferda ЬNSAL CANAY, Ramazan AVCIOĞLU, Ayten SALANTUR, Selami YAZAR, Bayram ЦZDEMİR, Mehmet Emin ALYAMAЗ³, Gцkhan KILIЗ, Muhsin İbrahim AVCI, Fatma Betьl SADE

YIELD AND QUALITY CHARACTERISTICS OF DURUM WHEAT GENOTYPES UNDER RAINFED CONDITIONS IN CENTRAL ANATOLIA REGION [Abstract] [Full text]

AYALA J. F. (in memoriam)

TWO REVOLUTIONS: COPERNICUS AND DARWIN [Abstract] [Full text]

EDITOR

CORRIGENDUM [Abstract] [Text]

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Published in „GENETIKA“ Vol. 55, No.2(2023), pp. 455-472

UDC 575. 633
https://doi.org/10.2298/GENSR2302455V
Original scientific article

GENOTYPIC SPECIFICITY OF SOYBEAN [Glycine max (L.) MERR.] PLASTID PIGMENTS CONTENT UNDER SOWING DATE AND INTERROW SPACING

Viliana VASILEVA^1*, Georgi GEORGIEV² and Vera POPOVIЖ³

¹Maize Research Institute, Knezha, Agricultural Academy, Bulgaria

²Experimental Station on Soybean and Cereals, Pavlikeni, Agricultural Academy, Bulgaria

³Institute of Field and Vegetable Crops, Novi Sad, Serbia

Abstract

Vasileva V., G. Georgiev and V. Popoviж (2023). Genotypic specificity of soybean [Glycine max (L.) merr.] plastid pigments content under sowing date and interrow spacing. - Genetika, Vol 55, No.2, 455-472.

Soybean [Glycine max (L.) Merr.] is a legume and the second most planted crop after maize in worldwide. The objective of this study was to evaluate the change in the total plastid pigments content in three soybean varieties depending on the date of sowing and the row spacing was studied during four consecutive years (2018-2021). The Avigea, Isidor and Richi varieties, sown on three dates (I - 28 March - 05 April; II - 19-24 April; III - 09-14 May) at row spacing of 25, 45 and 70 cm were studied in field conditions. The plastid pigments (chlorophyll a, chlorophyll b, carotenoids) was determined in fresh plant samples in the beginning of pod formation stage. The total content of plastid pigments and the chlorophyll a/chlorophyll b and chlorophyll a + chlorophyll b/carotenoids ratios were calculated. The total plastid pigments content varied by years and varieties depending on the date of sowing and row spacing. The date of sowing was found as a factor with stronger effect on the plastid pigments content compared to the row spacing. On average for the period it was found that the total plastid pigments content in the Avigea variety decreased compared to the first date of sowing at all three row spacing, most pronounced at 45 cm. The reduction reached 25.59% on the second date and up to 19.02% on the third sowing date. The strongest effect of the date of sowing on the plastid pigments content was found in the Richi variety on the third date of sowing, where the increase was up to 64.33% at a row spacing of 25 cm and up to 36.02% at a row spacing of 70 cm compared to the first date. The row spacing factor had a smaller effect - for Avigea variety a decrease of 12.31% was reported on the second sowing date and a row spacing of 45 cm compared to the 70 cm accepted row spacing and for Richi variety by 15.79% on the first date and row spacing 45 cm. The chlorophyll a/chlorophyll b ratio decreased at a row spacing of 25 at the second (for Isidor and Richi) and third sowing dates (for Avigea and Isidor).

Key words: soybean, plastid pigments, sowing date, row spacing, genotypic specificity, crop quality

Corresponding author: Viliana Vasileva, Maize Research Institute, 5835 Knezha, Agricultural Academy, Bulgaria, E-mail: viliana.vasileva@gmail.com

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Published in „GENETIKA“ Vol. 55, No. 2 (2023), pp.473-490

Serbia

UDC 575. 630
https://doi.org/10.2298/GENSR2302473J
Original scientific article

EVALUATION OF ACCESSION STRUCTURE AND GENETIC DIVERSITY

IN IRANIAN MILK THISTLE (Silybum marianum L.) BY ISSR MARKERS

Maryam Jamshidnia¹, Sedigheh ASGARY², Mahmoud RAFIEIAN-KOPAEI³

¹Department of Plant Breeding & Biotechnology, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran

²Isfahan Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran

³Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

Abstract

Jamshidnia M., S. Asgary, M. Rafieian-Kopaei (2023). Evaluation of accession structure and genetic diversity in Iranian milk thistle (Silybum marianum L.) by ISSR markers. - Genetika, Vol 55, No.2, 473-490.

The aim of this study was investigating genetic variations of Iranian S. marianum accessions using inter simple sequence repeat (ISSR) markers. Twenty-nine accessions from various Iran regions and a sample from Hungary were selected and evaluated by 19 ISSR primers. Clear banding pattern produced by 9 primers and totally 85 repeatable polymorphic bands were detected. ISSR7 and ISSR8 primers identified the most Polymorphic Information Content (PIC) with 100% among primers. In the tested accessions, polymorphism information content (0.45), polymorphic percentage (83 %), and Shannon’s information index (0.53) assessed high level of genetic variation. Based on Jaccards distances, cluster analysis molecular traits with unweighted pair group method with arithmetic mean (UPGMA) method were taken into consideration, and accessions were grouped into nine clusters and confirmed by principal coordinate analysis. Four clusters were identified by a Bayesian structure analysis, and 12 individuals were maintained inside the admixed clusters. According to the findings, ISSR marker system can be considered as a powerful tool for detection of genetic diversity of accessions in S. marianum. The results indicate existence of a high variation among Iranian S. marianum accessions to start the breeding programs.

Keywords: Asteraceae, Genetic variation, Inter simple sequence repeat, PCR, Silymarin.

Corresponding author: Maryam Jamshidnia, Department of Plant Breeding & Biotechnology, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran; Phone: +989132068624, E-mail: m.jamshidnia@stu.sanru.ac.ir

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Published in „GENETIKA“ Vol. 55, No.2 (2023), pp.491-503

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2302491C

Original scientific article

ISOLATION AND BIOINFORMATICS CHARACTERIZATION OF HORSE GRAM (Macrotyloma uniflorum (Lam.) Verdc.) HOMOLOGS (MuTFL1 and MuLFY) OF GENES CONTROLLING GROWTH HABIT

Basalapura Rangegowda CHANDANA, Sampangi RAMESH, Rotti KIRANKUMAR,

Gonal BASANAGOUDA and Mugali Pundalik KALPANA

Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, Bangalore, Karnataka, India

Abstract

Chandana B. R., S. Ramesh, R. Kirankumar, G.Basanagouda, M. Pundalik Kalpana (2023). Isolation and bioinformatics characterization of horse gram (Macrotyloma uniflorum (lam.) verdc.) homologs (MuTFL1 and MuLFY) of genes controlling growth habit. - Genetika, Vol 55, No.2, 491-503.

Growth habit (indeterminate/determinate) is one of the evolutionarily shaped, economically important plant architectural traits in grain legumes, including horse gram. Arabidopsis AtTFL1 and AtLFY genes and their homologs in other grain legume species are known to control growth habit. Taking cues from highly conserved domains in protein encoded by TFL1 and LFY genes, we isolated horse gram homologs (MuTFL1 and MuLFY) genes using reported degenerate primers designed to conserved domains. MuTFL1 and MuLFY homologs were isolated and sequenced using Sanger’s sequencing protocol. The nucleotide sequences of MuTFL1 and MuLFY homologs were translated to their corresponding amino acid sequences using “ExPASy” tool. BLASTx analysis of the translated amino acid sequences of MuTFL1 and MuLFY homologs showed high similarity with those of soybean and pigeon pea TFL1 and LFY homologs. Multiple sequence alignment of nucleotide sequences of MuTFL1 and MuLFY with those of related legumes and model species (Medicago sp. and Lotus japonicas) using “ClustalW” revealed the presence of four synonymous single nucleotide polymorphic (SNPs) sites in MuTFL1 and three non-synonymous SNP sites in MuLFY. These results suggest that not MuTFL1, but MuLFY could be the main regulator of growth habit in horse gram unlike in other non-model legumes where TFL1 was reported as the key gene controlling the growth habit. The substitution of glutamine in (determinate genotypes) with histidine (indeterminate genotypes) in LFY-coded protein appeared to be the cause for switch over from indeterminate to determinate growth habit in horse gram. These results are discussed in relation to strategies for breeding horse gram cultivars with desired growth habits.

Keywords: Determinate, homologs, indeterminate, LFY, TFL1

Corresponding author: Sampangi Ramesh, Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, Bangalore, Karnataka, India, Phone +919480704010, E-mail: ramesh_uasb@rediffmail.com

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Published in „GENETIKA“ Vol. 55, No2(2023), pp. 505-522

Serbia

UDC 575.633.15
https://doi.org/10.2298/GENSR2302505P

Original scientific article

GRAIN YIELD STABILITY ESTIMATES OF LATE MATURITY MAIZE HYBRIDS

Jovan PAVLOV¹, Nenad DELIЖ¹, Zoran ИAMDЋIJA¹, Gordana BRANKOVIЖ²,

Nataљa MILOSAVLJEVIЖ³, Nikola GRИIЖ¹, Sofija BOЋINOVIЖ¹

¹Maize Research Institute “Zemun Polje”, Belgrade, Serbia

²University of Belgrade, Faculty of Agriculture, Department of Genetics, Plant Breeding and Seed Science; Belgrade, Serbia

³University of Belgrade, Faculty of Agriculture, Department of Mathematics and Physics, Belgrade, Serbia

Abstract

Pavlov J., N. Deliж, Z. Иamdћija, G. Brankoviж, N. Milosavljeviж, N. Grиiж, S.Boћinoviж (2023). Grain yield stability estimates of late maturity maize hybrids. - Genetika, Vol 55, No.2, 505-522.

With global climate change including unpredictable geographic and temporal weather patterns that lead to significant genotype Ч environment interaction (GEI) the maize performance assessment would need to be complemented with stability analysis. The objectives of this study were: i) estimation of parametric and non-parametric stability indices for grain yield of late maturity maize hybrids, ii) assessing correlations and grouping of stability indices for grain yield of late maturity maize hybrids. The eight maize hybrids of FAO 700 maturity group (ZP1-ZP7, and check Ch) were evaluated for grain yield in the multi-environment trial including five locations in Serbia during 2020. and 2021. year. Stability analyses included twenty parametric and non-parametric indices, Principal Component Analysis (PCA) and Pearson’s correlations between stability indices. The sum of ranks for tested late maturity maize hybrids were in the interval from 37 (ZP6) to 146 (ZP4). The average rank was in the range from 1.8 (ZP6) to 7.3 (ZP4). The interval of variation for standard deviation of average ranks for tested maize hybrids was from 1.3 (ZP4, Ch) to 2 (ZP2) indicating satisfying accordance of utilized parametric and non-parametric stability measures. The highest average grain yield across all tested environments was observed for ZP1, which was second most stable hybrid. The most stable late maturity maize hybrid tested over ten environments was proven to be ZP6 with sum of ranks and average rank of 37 and 1.8, respectively, with standard deviation of average rank 1.5. PCA biplot showed two groups of parametric and non-parametric stability estimates: I group-coefficient of variation (CV_i), Eberhart and Russel’s regression coefficient (b_i), Perkins and Jinks’s regression coefficient (B_i), GEI variance component (θ_(i)), coefficient of determination (); II group-Eberhart and Russel’s deviation from regression , Wricke’s ecovalence Shukla’s stability variance Perkins and Jink’s deviation from regression , superiority measure the mean of the absolute rank differences of a genotype over all tested environments the variance among the genotype ranks over all tested environments (, the sum of the absolute deviations for each genotype relative to the mean of ranks , the sum of squares of rank for each genotype relative to the mean of ranks - Thennarasu non-parametric stability estimates, mean variance component (θ_i), Kang’s rank-sum (KR_i). Pearson’s correlation bring along the redundant stability indices and one can choose to use or, b_i or B_i, or θ_i,or θ_i.

Keywords: Zea mays L., multi-environment trial, genotype Ч environent interaction, univariate stability indices, correlations

Corresponding author: Jovan Pavlov, Maize Research Institute “Zemun Polje”, Slobodana Bajiжa 1, 11185 Belgrade, Serbia, email.jpavlov@mrizp.rs

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Published in „GENETIKA“ Vol. 55, No.2 (2023), pp. 523-536

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2302523K
Original scientific article

Solanum pseudo-capsicum EFFECTS ON Bax AND Bcl-2 GENE EXPRESSION

AND APOPTOSIS IN MCF-7 CELL LINE

Mahsa KAVOUSI* and Afsaneh DELFANI

Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran

Abstract

Kavousi M. and A. Delfani (2023). Solanum pseudo-capsicum effects on Bax and Bcl-2 gene expression and apoptosis in MCF-7 cell line. - Genetika, Vol 55, No.2, 523-536.

In the present study, the effect of Solanum pseudo-capsicum extract on MCF-7 cell line was investigated. Control cell line in this study was HFF skin fibroblast cell line. Cells have been exposed to the treatment for 24h, 48h and 72 hours respectively. Then, the viability of dual cell lines was assessed using MTT assay. Its results showed that 72-hour treatment with plant extract at IC₅₀ concentration had the greatest effect on cancer cell death. Flow cytometry results showed that 48 hours of treatment with plant extract at IC₅₀concentration, the highest rate of induced apoptosis occurs. Then, Real time PCR test was performed to measure changes in the expression of Bax and Bcl-2 genes The reference gene in this study was β-actin. The results of this study showed that in cancer cells treated with the plant extract, the expression of Bax pro-apoptotic gene increased and the expression of anti-apoptotic gene Bcl-2 decreased. Therefore, 72-hour treatment had the greatest effect on increasing Bax gene expression and decreasing Bcl-2 gene expression. Due to the lower side effects of plants compared to conventional chemical drugs used in the treatment of cancer, it is possible to take an effective step in the treatment of this disease by using new methods in identifying effective plant compounds and purifying them.

Keywords: Solanum pseudo-capsicum, MCF-7, Bax and Bcl-2, MTT assay, Real time PCR, Flow cytometry.

Corresponding author: Mahsa Kavousi, Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran, Tel/Fax: ++989127380035, Email: mkavoosi@iauet.ac.ir

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Published in „GENETIKA“ Vol. 55, No. 2(2023), pp. 537-552

Serbia

UDC 575.633
https://doi.org/10.2298/GENSR2302537P
Original scientific article

CONSERVATION PRACTICES AND ASSESSMENT OF SPATIAL AND TEMPORAL VARIABILITY IN RICE LANDRACES OF BASTAR REGION OF CHHATTISGARH, INDIA

Adikant PRADHAN¹, A. SAO², S. K. NAG³, Nilamani DIKSHIT^4*

¹S.G. College of Agriculture and Research Station, IGKV, Jagdalpur-494001, India

²College of Agriculture, IGKV, Raipur-492012, India

³Krishi Vigyan Kendra, IGKV, Bastar- 494005, India

⁴ICAR-Indian Grassland and Fodder Research Institute, Jhansi -284003, India

Abstract

Pradhan A., A. Sao, S. K. Nagand N. Dikshit (2023). Conservation practices and assessment of spatial and temporal variability in rice landraces of Bastar region of Chhattisgarh, India. - Genetika, Vol 55, No.2, 537-552.

Rice (Oryza sativa L.) is the staple food for more than half of the population in the world. In India, it provides food security and nutrition to more than two-third population and contributes 20-25% in Indian Agriculture. The state of Chhattisgarh is endowed with rich genetic diversity in cultivated and wild relatives of rice. Rice farming is practiced in several agro-ecologies ranging from rainfed upland rice ecosystem, rainfed lowland rice ecosystem, irrigated rice ecosystem and flood-prone rice ecosystem. Extensive field surveys and personal interviews were conducted in Bastar district of Chhattisgarh, India in the months of September to November during 2017 - 2019 and the rice samples were collected. During the field survey, a total of 193 varieties varying in seed characteristics viz. red, dark red, light red and white kernel types were collected. The grain size of the varieties ranges from slender to bold types and the grain length varied from long (8.1 to 11.0 mm), medium (5.0 to 8.0 mm) to small (2.5 to 4.0 mm). Red rice is much liked by the tribal people in the region due to its color and flavor with slow digestibility as it keeps them energetic for a longer time in the field. Out of the total varieties collected from the region, 41 varieties were recorded as red kernel type, 36 in brown rice category and five in light brown types. Regression line found in 2.67 by 2.25 (R²= 0.211) in seed and kernel color comparison.

Keywords: Conservation methods, Chhattisgarh, diversity, Traditional rice

Corresponding author: Nilamani Dikshit, Division of Crop Improvement, ICAR-Indian Grassland and Fodder Research Institute, Jhansi -284003, India, email nilamani.dikshit@icar.gov.in

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Published in „GENETIKA“ Vol. 55, No2(2023), pp 553-564

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2302553S
Original scientific article

CONTRARY TO KNOWN SILIBININ IS NOT AN ANTICANCER AGENT AND LIVER PROTECTIVE SUPPLEMENT

Deniz ЄUMNULU^*

Abstract

Єumnulu D. (2023). Contrary to known silibinin is not an anticancer agent and liver protective supplement. - Genetika, Vol 55, No.2, 553-564.

Silibinin is a flavinoid that is the main component of the milk thistle plant and is widely used around the world, thought to have liver protective and anticancer effects. In this study, contrary to what is generally known, it was aimed to show that silibinin is neither a healthy liver protective supplement nor an anticancer agent for hepatocellular cancer cells. For this purpose, molecular effects of silibinin were investigated in both HepG2 (hepatocellular cancer) and AML-12 (healthy liver) cells. The cytotoxic concentrations of silibinin investigated by MTT analysis. Cell proliferation by wound healing assays, intracellular apoptosis and cell division events demonstrated by fluorescent microscopy imaging via Annexin V/ PI and Hoechst 34580 staining. Gene expression level changes were investigated by real-time polymerase chain reaction. In the study, IC50 values were calculated as 739.9 mM for AML-12 and 1.35 M for HepG2 in 24 h, 529.23 µM for AML-12 and 15.51 mM for HepG2 in 48-h of silibinin administration. From these data, IC50 value of 48 h of silibinin administration for the AML-12 cell line resulted a decrease in AML-12 cell quantity, whereas an increase in HepG2 cells. Fluorescent staining studies show that, there was an intense proliferation in the HepG2 cells, whereas an important apoptotic effect induced in the AML-12 cell line as a result to 529.23 µM silibinin application. Also, all proliferation and oncogene expression levels were incrased in HepG2 cells, but expression levels of Akt, ErbB2 were decreased in AML-12 cells, whereas APEX1 DNA repair, CuZn-SOD oxidative stress gene expression levels were increased. As a result, application of 529.23 µM silibinin was found to be cytotoxic for AML-12 cells as well as proliferative effect on HepG2.

Keywords: AML-12, hepatocellular carcinoma, HepG2, silibinin, MTT.

Corresponding author: Deniz Єumnulu, Technology Research Development Application and Research Center (TЬTAGEM), Trakya University, Edirne, Turkey,Phone: +90 (546) 938 05 92, E-mail: denizsumnulu@trakya.edu.tr

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Published in „GENETIKA“ Vol. 55, No.2 (2023), pp.565-581

Serbia

UDC 575.633.11
https://doi.org/10.2298/GENSR2302565F
Original scientific article

GENETIC EFFECTS OF Rht-B1b AND Rht-D1b DWARFING ALLELES ON PLANT HEIGHT AND AGRONOMIC TRAITS OF BREAD WHEAT (Triticum aestivum L.) BREEDING LINES

Zine El Abidine FELLAHI^1,2,*, Abderrahmane HANNACHI³, Susanne DREISIGACKER⁴, Deepmala SEHGAL⁴, Amor YAHYAOUI⁴, Hamenna BOUZERZOUR^2,5

¹Department of Agronomic Sciences, Faculty of Natural, Life and Earth Sciences and the Universe, University Mohamed El Bachir El Ibrahimi of Bordj Bou Arreridj, 34030, Algeria

²Valorization of Natural Biological Resources Laboratory, Faculty of Natural and Life Sciences, University of Ferhat Abbas Sйtif-1, 19000, Algeria

³National Agronomic Research Institute of Algeria (INRAA), Unit of Setif, 19000, Algeria⁴International Maize and Wheat Improvement Center (CIMMYT) km. 45 Carretera Mexico-Veracruz CP 56130. Texcoco, Estado de Mexico, Mexico

⁵Department of Ecology and Plant Biology, Faculty of Natural and Life Sciences, University of Ferhat Abbas Setif-1, 19000, Algeria

Abstract

Fellahi Z., A. Hannachi, S. Dreisigacker, D. Sehgal, A. Yahyaoui, H. Bouzerzour (2023). Genetic effects of Rht-B1b and Rht-D1b dwarfing alleles on plant height and agronomic traits of bread wheat (Triticum aestivum L.) breeding lines. - Genetika, Vol 55, No.2, 565-581.

Plant height is an important agronomic trait related to plant architecture and grain yield in wheat. We investigated the effects of the gibberellic acid (GA)-insensitive Rht-B1b and Rht-D1b semi-dwarfing alleles in a set of 599 F_4:5spring wheat breeding lines grown in rainfed and supplemental irrigation conditions at Setif semi-arid high plateaus region (Algeria). Genotyping of diagnostic markers was performed using Kompetitive Allele Specific PCR assays. The Rht-B1b mutant allele was present in 66% of the evaluated lines, while the Rht-D1b allele was found only in 8% of the lines. Relative to wild-type, Rht-B1b or Rht-D1b alone reduced plant height by 16.0 and 16.3% in rainfed, and by 20.7 and 21.8% in irrigated environments, respectively. The two dwarfing alleles when combined decreased height by 39.3 and 56.6% in rainfed and irrigated conditions, respectively. The semi-dwarf breeding lines generally outperformed the tall genotypes for yield and yield components including the number of grains per spike and harvest index irrespective of environment. Variation in heading date under the influence of dwarfing alleles has not been found. In addition, above-ground biomass was reduced in most of the short statured lines, compared to their tall counter parts across environments. We also demonstrate clear yield and yield components penalty for Rht-B1b + Rht-D1b double dwarf backgrounds.

Keywords: Triticum aestivum, reduced height genes, pleiotropic effects, plant height, yield components.

Corresponding author: Zine El Abidine Fellahi, Department of Agronomic Sciences, Faculty of Natural, Life and Earth Sciences and the Universe, University Mohamed El Bachir El Ibrahimi of Bordj Bou Arreridj, 34030, Algeria. E-mail : zinou.agro@gmail.com / zineelabidine.fellahi@univ-bba.dz

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Published in „GENETIKA“ Vol. 55, No. 2(2023), pp. 583-595

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2302583W
Original scientific article

F₁ PROGENY OF ‘RUBY SEEDLESS’Ч ‘HONGQITEZAO’ DEVELOPED

AND SELECTED USING EMBRYO-RESCUE AND MOLECLAR MARKERS

Yong WANG¹, Yuling L.LI¹, Feng SUN^*, Guohong H. WU, Aizezi Z. SUlAIMAN, Shijian J. BAI, Ronghua H. ZHAO, Pingfeng F. GUO

Research Institute of Grape and Melon of Xinjiang Uygur Autonomous Region, Shanshan City, Xinjiang Uygur Autonomous Region, 838200, China

Abstract

Wang Y., Y. L. Li, F. Sun, G. H. Wu, A. Z. Sulaiman, S. J. Bai, R. H. Zhao, P. F. Guo (2023). F₁ progeny of ‘Ruby seedless’Ч ‘Hongqitezao’ developed and selected using embryo-rescue and molecular markers. - Genetika, Vol 55, No.2, 583-595.

Seedless grapes play an important role in fresh food and dry production. New varieties breeding by hybridization with seedless varieties as female parents is the most effective way to cultivate seedless varieties. However, the embryos of seedless varieties cannot develop normally, so it is difficult to obtain hybrid offspring as hybrid female parent. Moreover, grape is a perennial tree species with highly heterozygous genes, with long breeding cycle and low efficiency. In this study, embryo rescue technique was used to cultivate hybrid offspring by crossing with ‘Ruby Seedless’ as female parent and ‘Hongqitezao’ as male parent, so as to solve the problem that seedless varieties cannot be female parent; and molecular markers technology was used to carry out assisted breeding research to solve the problems of long cycle and low efficiency. TP-M13-SSR technique was used to carry out authenticity breeding. SCAR marker SCF27 was used to detect the seedless traits of hybrid plants, phenotypic traits was used to verify the results of molecular markers, and seedless trait-related SSR markers VMC7F2, VrSD10 and P3_VvAGL11 was used to detect and verify the genotypes of individual plants with inconsistent detection results by the two methods. The results showed that a total of 384 hybrid offspring were finally obtained, and the hybridization rate was 84.43%. The seed phenotypes of 163 fruit-bearing plants were identified, and the coincidence rate of genotypic and phenotypic analyses was 95.71%. Additionally, 305 F₁ plants were detected using the SCF27 marker, and the abortion rate was 64.92%. This study shows that embryo rescue is an effective method for breeding seedless grape cultivars, and the application of molecular markers could facilitate the early identification of hybrid traits, and improve breeding efficiency.

Keywords: Grapevine, seedless breeding, F₁ progeny, embryo rescue, molecular marker

Corresponding author: Feng Sun, Research Institute of Grape and Melon of Xinjiang Uygur Autonomous Region, Shanshan County, Xinjiang Uygur Autonomous Region, 838200, China, Telephone：0995-8382064, E-mail: sunfeng8302871@163.com

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Published in „GENETIKA“ Vol. 55, No.2 (2023), pp. 597-610

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2302597U
Original scientific article

MORPHOLOGICAL AND MOLECULAR IDENTIFICATION OF HYBRID APRICOT GENOTYPES OBTAINED BY INTRA-SPECIFIC HYBRIDIZATION

Aydın UZUN¹, Emine DIRIM^2*, Mehmet YAMAN³

¹Erciyes University, Faculty of Agriculture, Department of Horticulture, Kayseri, Turkey

²Erciyes University, Faculty of Agriculture, Department of Agricultural Biotechnology, Kayseri, Turkey

³Erciyes University, Faculty of Agriculture, Department of Horticulture, Kayseri, Turkey

Abstract

Uzun A., E. Dirim, M. Yaman (2023). Morphological and molecular identification of hybrid apricot genotypes obtained by intra-specific hybridization. - Genetika, Vol 55, No.2, 597-610.

Apricot is one of the important stone fruits produced in the world. In this study, genetic variation was investigated based on SRAP marker technique and morphological data in 120 genotypes and parent plants obtained by inbreeding in some apricot cultivars such as Ninfa, Proce de Tyrinthe, Palstein, Hacıhaliloğlu, Hasanbey, Aprikoz and Kabaaєı. In the study, 15 different combinations of SRAP markers were used and a total of 105 scoreable bands were obtained and 76 of them were determined as polymorphic. The average base lengths of these primers are between 200-1700 bp, the average number of polymorphic bands per primer is 7.0. The mean polymorphism value is 71.64%. The similarity coefficient in the dendrogram created according to the UPGMA method differed between 0.61 and 0.96. According to the dendrogram, 2 main groups were formed. The closest genotypes have a similarity index of 0.96. In the morphological characterization analysis, 120 hybrid individuals were examined with 19 UPOV criteria. No variation was found in terms of petiole nectarium number, dominant numbers and petiole shape characteristics. Variations between hybrids were determined in terms of 16 characteristics such as plant development, plant habitus, amount of branching in the tree, leaf and shoot characteristics. 119 hybrids showed ‘reddish brown’ coloration, 87 hybrids medium leaf tip, and 116 plants 'double dentate' in terms of incisions of margin. 84 hybrids showed medium petiole incision, 101 hybrids showed medium petiole thickness, and 117 plants showed weak petiole anthocyanin coloration. It is predicted that the morphological and molecular analyzes of the hybrid plants will enable the development of new cultivars and rootstock candidate genotypes. With the results of this study, the genetic variations and morphological classifications determined in the genotypes will guide the further studies on hybrid breeding programs in apricots.

Keywords: apricot, hybridization, morphological characterization, SRAP marker, UPOV

Corresponding author: Emine Dirim, Erciyes University, Faculty of Agriculture, Department of Agricultural Biotechnology, Kayseri, Turkey; E-mail: e.arikan.drm@gmail.com

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Published in „GENETIKA“ Vol. 55, No. 2(2023), pp. 611-629

Serbia

UDC 575.633.15
https://doi.org/10.2298/GENSR2302611D
Original scientific article

SEED QUALITY OF RECIPROCAL MAIZE SINGLE CROSSES AND THEIR PARENTAL INBRED LINES

Olivera DJORDJEVIЖ MELNIK^1*, Sofija BOЋINOVIЖ¹, Radmila VUKADINOVIЖ²,

Marija MILIVOJEVIЖ², Tanja PETROVIЖ², Tomislav ЋIVANOVIЖ³, Jelena VANИETOVIЖ¹

¹Breeding department, Maize Research Institute Zemun Polje, Zemun Polje - Belgrade, Republic of Serbia

²ISTA attested Seed testing laboratory, Maize Research Institute Zemun Polje, Zemun Polje - Belgrade, Republic of Serbia

³ Faculty of Agriculture, University of Belgrade

Abstract

Djordjeviж Melnik O., S. Boћinoviж, R. Vukadinoviж, M. Milivojeviж, T. Petroviж, T. Ћivanoviж, J. Vanиetoviж (2023). Seed quality of reciprocal maize single crosses and their parental inbred lines. - Genetika, Vol 55, No.2, 611-629.

Five direct and reciprocal early hybrids, five direct and reciprocal late hybrids and a total of their 12 parental inbred lines were used in order to test reciprocal effects among hybrids in early germination phase and seed quality of their parental inbred lines. Five hybrids were of early maturity FAO 300-400 group, and five of late maturity FAO 500-600 group. In the early group one line of Lancaster origin (L1) was in common, and in direct crosses used as male parent, while in reciprocal crosses as female parent. The same case was with late hybrids where another Lancaster line (L2) was in common. In 2015 and 2016 all the material was multiplied in hand pollination and after six months of storage three tests according to ISTA standards were performed: standard germination test (SGT), cold-test (CT) and vigor radicle test (RE). According to the Analysis of variance, a strong reciprocal effect was found among early hybrids for four out of five parameters in RE and SGT, while it was found only for RE in late hybrids. However, in CT none of the parameters had significant reciprocal effect. Two tested Lancaster inbreds were superior in the hybrids over non-Lancaster lines when used as maternal components, while as lines per se they did not display such superiority. Mid-parent heterosis (MPH) ranged from -62.32% to 211.11% in RE test, with an average of 44.26%; from -10.15% to 17.93% in SGT test, with an average of 6.27%, and from -5.31% to 4.49% in CT, with an average of 0.64%. Significant differences of MPH were found for nine pairs of reciprocal hybrids for RE, three pairs for SGT and seven pairs for CT. This shows that reciprocal differences in MPH were higher as the applied level of stress during early seed growth raised. All the results showed very complicated mode of inheritance of tested traits in the early developmental stages of young plants, including the phenomenon of heterosis which is expressed already at the germination stage.

Keywords: heterosis, ISTA, reciprocal effects, seed quality testing, Zea mays L.

Corresponding author: Olivera Djordjeviж Melnik, Breeding department, Maize Research Institute Zemun Polje, Slobodana Bajiжa 1, 11185 Zemun Polje - Belgrade, Republic of Serbia, Phone.: ++381648406056, E-mail: djolivera@mrizp.rs

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Published in „GENETIKA“ Vol. 55, No2 (2023), pp631-645

Serbia

UDC 575.633
https://doi.org/10.2298/GENSR2302631A
Original scientific paper

MOLECULAR CHARACTERIZATION OF SEED LONGEVITY IN SOYBEAN

[Glycine max (L.) Merrill] USING SSR MARKERS

Amit T. ADSUL^1*, Vivek P. CHIMOTE², Dilip S. THAKARE¹, Milind P. DESHMUKH³

¹Department of Genetics and Plant Breeding, Mahatma Phule Krishi Vidyapeeth, Rahuri 413722 Ahmednagar (MH) India

²Department of Botany, State Level Biotechnology Centre, Mahatma Phule Krishi, India.

³Agriculture Research Station, Kasbe Digraj 416310 Sangli (MH) India

Abstract

Adsul A. T., V. P. Chimote, D. S. Thakare, M.P. Deshmukh (2023). Molecular characterization of seed longevity in soybean [Glycine max (L.) Merrill] using SSR markers. - Genetika, Vol 55, No.2, 631-645.

Soybean is the most important oilseed in the tropical and subtropical parts of the world unfortunately has poor competence for seed longevity. It’s strongly affected by environment (Temperature and RH) during seed formation, harvest and storage. Tagging of seed longevity associated markers in the crosses (Birsa Soya1 Ч DS-228) which are good and poor storer genotypes resp. by using 26 SSR primers in genotypes contrasting for these traits in soybean. The polymorphism was observed between genotypes differing for seed longevity trait for six primer pairs, viz; Sat_162, Satt632, Sat_202, Satt523, Satt184 and Satt460, among them seed coat hardiness QTL, SCH 1-2 linked Satt632 amplified distinct markers. Bulked segregant analysis was carried out by using SSR markers in segregating 93 F2 populations. The Satt632-237 bp marker was further validated in individual F2 plant population. The genotype, Birsa Soya1 and it segregants Satt632-237 bp marker was found to be closely associated with high seed longevity in soybean. Hence, the primer Satt632 is impending marker for marker assisted breeding for high seed longevity marker to be linked to seed coat hardiness loci qSCH1-2 like gene present on chromosome A2. Seed longevity trait is governed by one or few gene with observed segregation ratio of 3.65:1 as against expected segregated ratio 3:1. Candidate gene analysis at qSCH1-2 loci, indicated for role of either a brassinosteriod regulated protein or pentatricopeptide repeat gene to regulate seed longevity.

Keywords: Accelerated aging, Seed Coat Hardiness, Seed longevity, SSR, Soybean

Corresponding author: Amit T. Adsul, Department of Genetics and Plant Breeding, MPKV, Rahuri 413722 Ahmednagar (MH) India. E-mail: amitadsul444@gmail.com

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Published in „GENETIKA“ Vol. 55, No. 2(2023), pp. 647-654

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2302647S
Original scientific article

INVESTIGATION OF THE CC CHEMOKINE RECEPTOR 5 (CCR5) DELTA32

AND GENE VARIANTS IN HIV INFECTED PATIENTS

Ayesha SADDIQA¹, Asif NADEEM^2*, Maryam JAVED¹, Tahir YAQUB³, Nadia MUKHTAR³, Jahanzaib AZHAR², Muhammad Salman TARIQ⁴

¹Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences Lahore 54000, Pakistan

²Department of Biotechnology, Virtual University of Pakistan, Lahore 54000, Pakistan

³Institute of Microbiology, University of Veterinary and Animal Sciences Lahore 54000, Pakistan

⁴ Faculty of Pharmacy, Department of Pharmacology, the University of Lahore

Abstract

Saddiqa A., A.Nadeem, M. Javed, T. Yaqub, N. Mukhtar, J. Azhar, M. S.Tariq (2023). Investigation of the cc chemokine receptor 5 (ccr5) delta32 and gene variants in HIV infected patients. - Genetika, Vol 55, No.2, 647-654.

CCR5 is an important CC chemokine receptor for the normal entry of Human Immunodeficiency Virus (HIV) in host cells. Mutations in this gene have been linked with delayed HIV infection, therefore, the current case-control study was conducted to identify genetic polymorphism in CCR5 gene in Pakistani population and to evaluate its association with resistance for HIV infections. All regulations of MOOSE and Helsinki Declaration were strictly followed during the entire study. DNA was extracted from the blood samples of HIV positive and HIV negative patients. Extracted DNA was amplified for CCR5 gene by PCR. Amplified product was sequenced to screen out polymorphism mutation, including most prevalent 32 base-pairs deletion through Bioedit. Novel SNP in 5’UTR region was identified and examined by CRYP-SKIP server. None of the studied samples demonstrated any previously reported polymorphisms. CRYP-SKYP server predicted that this polymorphism has no effect on splicing or transcription of CCR5 gene. Genetic Polymorphism of delta32 mutation in CCR5 gene was found in Pakistani individuals. To the best of our knowledge, this is the first report from this region. However, large scale studies should be conducted for extensive view of the association of delta32 mutation in CCR5 gene and resistance for HIV infection.

Keywords: Human Immunodeficiency Virus, delta32 mutation, Genetic Polymorphism, Pakistan, CCR5 gene

Corresponding author: Asif Nadeem,Department of Biotechnology, Virtual University of Pakistan, E-mail: asif.nadeem@vu.edu.pk

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Published in „GENETIKA“ Vol. 55, No2(2023), pp. 655-672

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2302655A
Original scientific article

THE EFFECTS OF MC4R AND CACNA2D1 GENE POLYMORPHISMS ON CARCASS TRAITS AND MARBLING SCORE IN TURKISH NATIVE CATTLE BREEDS

AND THEIR CROSSBREDS WITH THE HOLSTEIN-FRIESIANS

Sena ARDICLI¹and Fazli ALPAY²

¹Department of Genetics, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa, 16059, Turkey

²Department of Animal Science, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa, 16059, Turkey

Abstract

Ardicli S.and F. Alpay (2023). The effects of MC4R and CACNA2D1 gene polymorphisms on carcass traits and marbling score in Turkish native cattle breeds and their crossbreds with the Holstein-Friesians. - Genetika, Vol 55, No.2, 655-672.

Carcass and beef quality traits are economically important traits and are expressed by multiple genes. The effects of the MC4R c.856C>G and CACNA2D1 c.2027A>G polymorphisms on carcass and meat quality traits are limited. Therefore, this study aimed at evaluating the association of bovine MC4R and CACNA2D1 markers with carcass characteristics and meat quality. A total of 102 cattle including Turkish Grey Steppe, East Anatolian Red, Zavot, and their F₁ crossbreds with the Holstein-Friesians were genotyped using the PCR-RFLP method. The phenotypic traits measured were slaughter weight, hot carcass weight, chilled carcass weight, dressing percentage, chilling loss, carcass fatness score, carcass pH₂₄, and marbling score. Statistical analyses were performed using linear mixed models in the entire cattle population and also from a breed-specific aspect. Population genetics and diversity indices were also estimated. Results revealed that the genetic markers in this study are reasonably informative for the studied cattle population and exhibit an intermediate genetic diversity. Concerning the MC4R c.856C>G polymorphism, there was no significant association with any of the traits analyzed, indicating that this MC4R c.856C>G is not a desirable marker for carcass traits and marbling. Here, we report a novel association between the CACNA2D1 c.2027A>G polymorphism and marbling score. The GG genotype was characterized by higher marbling scores (P<0.05). Bovine CACNA2D1 is located on BTA 4, which consists of important QTLs for marbling, and hence, the evaluation of genetic markers within this genomic region may reveal novel genetic associations through meat quality.

Keywords: association analysis , carcass traits, cattle, MC4R, CACNA2D1

Correspondin author: Sena Ardicli, DVM, PhD,Associate Professor,Department of Genetics, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa, 16059, Turkey, sardicli@uludag.edu.tr,Tel: +905414875448

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Published in „GENETIKA“ Vol. 55, No. 2(2023), pp. 673-688

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2302673S
Original scientific article

ASSOCIATION OF SINGLE NUCLEOTIDE POLYMORPHISMS OF GH, LEP, MSTN GENES WITH GROWTH CHARACTERISTICS IN MEAT-WOOL AND WOOL SHEEP

Larisa Nikolayevna SKORYKH^1*, Nadezhda Sergeevna SAFONOVA¹, Arslan Akhmetovich OMAROV¹,

Nina Ivanovna EFIMOVA¹, Konstantin Alexsandrovich KATKOV¹, Violeta Caro PETROVIC², Natalia Igorevna KIZILOVA³

¹North Caucasus Federal Agrarian Research Centre, Mikhailovsk, Russia

²Institut for Animal Husbandry, Belgrade, Serbia

³Stavropol State Agrarian University, Stavropol, Russia

Abstract

Skorykh Nikolayevna L., N. Sergeevna Safonova, A. Akhmetovich Omarov, N. Ivanovna Efimova, K. Alexsandrovich Katkov, V. Caro Petrovic, N. Igorevna Kizilova (2023). Association of single nucleotide polymorphisms of GH, LEP, MSTN genes with growth characteristics in meat-wool and wool sheep. - Genetika, Vol 55, No.2, 673-688.

The main trend in the development of sheep breeding in the last decade all over the world has become a constant increase in the production of mutton. This study was designed to study polymorphisms of genes GH, LEP, MSTN in two breeds of North Caucasian Meat-Wool and Soviet Merino sheep in Russia, as well as to identify potential single nucleotide polymorphisms (SNPs) associated with growth traits to improve the genetic potential of sheep. Parts of the GH, LEP, MSTN genes were amplified in North Caucasian Meat-Wool and Soviet Merino sheep to identify SNPs by Sanger sequencing and using a polymerase chain reaction protocol. These genotypes were correlated with meat traits such as birth weight, growth rate, weaning weight. Genetic and variance analysis of the data obtained was also carried out. Sheep age and parity had a significant effect (p <0.05) on birth weight, growth rate and weaning weight. Sequencing revealed missense mutations in the somatotropin, leptin and myostatin genes in the structure of the sheep genome of the studied breeds. Missense mutations of the GH gene (c.476G> A) and the LEP gene (c.541G> T), as well as a synonymous replacement of the MSTN gene (c. 212C> A), were revealed. According to the results of studies in the North Caucasian Meat-Wool sheep, three GH^CC, GH^CT, GH^TT genotypes for the GH gene and three LEP^GG, LEP^GT, LEP^ТТ genotypes for LEP were identified. The studied region of the MSTN gene in sheep of the North Caucasian meat and wool breed turned out to be monomorphic. According to the results of studies in Soviet Merino sheep, three genotypes GH^CC, GH^CT, GH^TT for the GH gene, three genotypes MSTN^СС, MSTN^СА, MSTN^АА for the MSTN gene, two genotypes LEP^GG, LEP^GT for the LEP gene were established. The analysis of associations showed a significant effect (p <0.05) of the GH^СТ and LEP^GT genotypes on the signs of sheep growth. Interestingly, the presence of the T-allele in sheep of the North Caucasian Meat-Wool breed tended to increase in weight during weaning (+2.2 kg) both for the GH gene and for the LEP gene (+ 1.6 kg). Interestingly, similar results were observed in sheep of the Soviet Merino breed, where the missense mutation led to an increase in weaning weight (+ 1.2 kg) for the GH gene and for the LEP gene (+1.3 kg). A synonymous MSTN gene substitution does not lead to a substitution of the coding amino acid, but may further lead to gene expression. The GH, LEP, and MSTN genes are proposed markers for improving growth traits in meat-wool and wool sheep, which will increase the ability to understand the genetic architecture of the genes underlying SNPs that influence such traits.

Keywords: growth hormone gene (GH), leptin gene (LEP), myostatin gene (MSTN), sheep

Corresponding author: Larisa Nikolayevna Skorykh, North Caucasus Federal Agrarian Research Centre, Mikhailovsk, Russia, E-mail: lara02.76@mail.ru

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Published in „GENETIKA“ Vol. 55, No. 2(2023), pp. 689-705

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2302689A
Original scientific article

ASSOCIATION STUDY OF RS323344 IN TEX15 WITH NON-OBSTRUCTIVE AZOOSPERMIA IN IRANIAN POPULATION

Bita ALIASGHAR¹, Saba SANE¹, Masoud SHEIDAI^2*, Fahime KOOHDAR², Naser KALHOR³

¹Department of Cell and Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran

²Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

³Department of mesenchymal stem cell, Academic Center for Education, Culture, and Research, Qom branch, Iran

Abstract

Aliasghar B., S. Sane, M. Sheidai, F. Koohdar, N. Kalhor (2023). Association study of rs323344 in tex15 with non-obstructive azoospermia in Iranian population. - Genetika, Vol 55, No.2, 689-705.

Infertility is a global health issue that affected approximately 15% of couples across the world. Genetic and environmental factors have a significant role in the manifestation of male infertility. Genetic factors contribute to 15% – 30% of male infertility. Testis expressed 15 (TEX15) gene plays an important role in chromosome synapsis, DNA double-strand break repair, and meiotic recombination. DNA double-strand break repair is required during homologous chromosome pairing and chromosome synapsis. The TEX15 gene is essential for normal gametogenesis and maintenance of genome integrity. The present study was carried out to investigate the association of SNP rs323344 (p.Leu1720Val, c.5158 T>G) in the TEX15 gene with azoospermia male infertility among the Iranian population. To conduct this case-control study, a total of 100 Iranian men, including 50 infertile cases diagnosed with non-obstructive azoospermia and 50 fertile controls from 5 different ethnics, were recruited. SNP rs323344 was genotyped using the polymerase chain reaction-restriction fragment length (PCR-RFLP) method. Furthermore, a number of samples were sequenced by the Sanger sequencing method. Variants rs323344 in TEX15 showed the lack of statistically significant differences in genotype distributions between men diagnosed with azoospermia infertility and the fertile group in our studied samples in Iran. In general, our studied genome segment demonstrated a high degree of conservation and a low level of nucleotide variability. Our findings indicated that the SNP rs323344 and its neighboring nucleotides play no role in male infertility. This variant cannot be considered a biomarker for azoospermia in the Iranian population. Further studies with larger sample sizes and different ethnic backgrounds are needed to confirm the present findings.

Keywords DSB repair, male infertility, non-obstructive azoospermia, rs323344, Testis-expressed 15 (TEX15)

Corresponding author: Masoud Sheidai, Faculty of Life Science and Biotechnology, Shahid Beheshti University, Tehran, Iran, E-mail: msheidai@yahoo.com, msheidai@sbu.ac.ir. Tel: +989122593378.

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Published in „GENETIKA“ Vol. 55, No. 2(2023), pp.707-718

S. Bajiжa 1, 11185 Belgrade-Zemun

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2302707S
Original scientific article

ANALYSIS OF GENETIC VARIATIONS IN TURKISH DOMESTIC GOOSE POPULATIONS AND CHINESE Ч EMBDEN CROSSES USING MICROSATELLITE MARKERS

Zeynep DEMİRTAЄ ЄAHİN and Levent MERCAN*

Agricultural Biotechnology Department, Faculty of Agriculture, Ondokuz Mayıs University, 55139, Samsun, Tьrkiye

Abstract

Єahin Demirtaє Z, and L.Mercan (2023). Analysis of genetic variations in Turkish domestic goose populations and Chinese Ч Embden crosses using microsatellite markers. - Genetika, Vol 55, No.2, 707-718.

The study investigates the intra-population and inter-population genetic variations of the domestic goose populations of Tьrkiye and the commercial Chinese - Embden cross genotypes using microsatellite markers. DNA samples were collected from 110 geese of four different populations. The populations' genetic diversity is assessed using the Ans02, Ans17, Ans25, Aalm1b, Aph19b, and TTUCG5 microsatellite loci. The highest number of alleles were detected at the Ans25 (28) locus, while the Aph19b (14) locus had the lowest. Wright’s F-statistics are calculated separately for each locus without discriminating against the populations. The F_ST value varied between 0.038 and 0.105 and had positive values in all loci. The number of migrants (N_m) ranged from 2.12 to 6.34. Structure and principal coordinates analysis indicated that the Native population had distinct characteristics from the other populations. The number of shared alleles among populations is accepted as an indicator of genetic erosion in native goose populations. This study is one of the first reports that exotic breeds genetically polluted Turkish native populations.

Keywords: Diversity, DNA, goose, marker, structure

Corresponding author: Levent Mercan, Agricultural Biotechnology Department, Faculty of Agriculture, Ondokuz Mayıs University, 55139, Samsun, Tьrkiye. E-mail: lmercan@omu.edu.tr, Phone: +90 362 312 19 19

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Published in „GENETIKA“ Vol. 55, No. 2(2023), pp719-728

S. Bajiжa 1, 11185 Belgrade-Zemun

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2302719C
Original scientific article

GENETIC VARIANTS OF ENDOTHELIAL NITRIC OXIDE SYNTHASE (eNOS4a/b) AND SUSCEPTIBILITY TO RENAL CELL CARCINOMA

IN A TURKISH POPULATION

Gozde CEYLAN^1*, Sefika Nur GUMUS¹, Sule SECKIN¹, Oner SANLI²,

Selcuk ERDEM², Canan KUCUKGERGIN¹

¹Department of Medical Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey

²Department of Urology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey

Abstract

Ceylan G., S. Nur Gumus, S. Seckın, O. Sanlı, S. Erdem, C. Kucukgergın (2023). Genetıc varıants of endothelıal nıtrıc oxıde synthase (eNOS4a/b) and susceptıbılıty to renal cell carcınoma ın a Turkısh populatıon. - Genetika, Vol 55, No.2, 719-728.

Nitric oxide (NO), plays a significant part in biological processes. Endothelial nitric oxide synthase (eNOS), the enzyme that catalyzes the generation of NO in endothelium, contains genetic polymorphisms that have been linked to an increased risk of developing cancer. The purpose of this investigation was to ascertain whether there is any connection between renal cell carcinom (RCC) and the eNOS 4a/b gene polymorphism. This study included 94 patients (mean age:54.2±10.5 years) diagnosed with histopathologically confirmed RCC and 188 healthy controls (mean age:56.7±11.1 years). eNOS 4a/b gene polymorphism was examined in DNA samples taken from patients and healthy controls using polymerase chain reaction (PCR) and agarose gel electrophoresis methods. The patient and control groups did not differ statistically significantly in terms of age or body mass index. The most frequent genotype of eNOS 4a/b gene polymorphism is bb genotype in a Turkish population. When compared to the control group, patients with RCC had significantly higher rates of the aa and ab genotypes (p= .018 and p= .000, respectively). There was no discernible difference in the eNOS 4a/b gene polymorphism between patients with high-grade and advanced-stage disease and those with low-grade and stage disease. We suggest that the eNOS 4a/b gene polymorphism may be effective in the onset of renal cell cancer, but it is not effective in development.

Keywords: eNOS 4a/b, nitric oxide, polymorphism, polymerase chain reaction, renal cell carcinoma

Corresponding author: Gozde Ceylan, Department of Medical Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey, Tel.: +905079328999, Fax: 0212 414 21 89,E-mail: cyln.gozde@hotmail.com

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Published in „GENETIKA“ Vol. 55, No. 2(2023), pp. 729-741

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2302729V
Original scientific article

EVALUATION OF THE IRISPLEX SYSTEM FOR EYE COLOUR PREDICTION

IN THE SERBIAN POPULATION

Marija VUKOVIC^*,Milica MIHAJLOVIC SREJIC^*, Katarina SAPIC, Vanja TANASIC,

Verica RADOJICIC, Milica KECKAREVIC MARKOVIC, Miljana KECMANOVIC,

Dusan KECKAREVIC

Centre for Forensic and Applied Molecular Genetics, Faculty of Biology, University of Belgrade, Belgrade, Serbia

*equally contributed

Abstract

Vukovic M.,M. Mihajlovic Srejic, K. Sapic, V. Tanasic, V. Radojicic, M. Keckarevic Markovic, M. Kecmanovic, D. Keckarevic (2023). Evaluation of the Irisplex system for eye colour prediction in the Serbian population. - Genetika, Vol 55, No.2,729 -741.

DNA-based prediction of a physical appearance, also known as DNA phenotyping, is a rapidly developing field with great potential for solving difficult forensic investigations. Externally visible characteristics (EVCs), such as eye colour, are easily recognised and genetically determined. Analysis of highly informative single nucleotide polymorphisms (SNPs) encompassing trait-associated genes provides information about the phenotype of an unknown individual, which is critical in cases where the standard STR profile is not useful. The IrisPlex assay was developed based on genotype data from 3804 Dutch Europeans, with the goal of accurately predicting brown, blue, and undefined eye colours using the six SNP markers alone. This assay has been validated in several studies and has shown high accuracy of prediction for brown and blue eye colours in most European populations. The aim of this work was to evaluate IrisPlex in the Serbian population and to determine if there are possible discrepancies in prediction accuracy compared to the previously published European data. Therefore, we performed the IrisPlex analysis on 65 Serbian individuals. Our results showed that the prediction of eye colour was accurate for about 57% of the respondents, which did not change significantly by applying the 0.7 threshold. The IrisPlex system performed well in predicting blue and brown eye colour in the Serbian sample, achieving high sensitivity levels. However, this model appeared to be unsuitable in the prediction of undefined eye colour, which was a common phenotype in our sample. This low sensitivity suggests a diverse and possibly population-specific genetic background of undefined eye colour. Given the large genetic diversity of the Serbian population, further work on a larger Serbian sample with more SNPs analysed is needed to reveal genetic variants mainly associated with this trait.

Keywords: DNA phenotyping, Eye colour prediction, IrisPlex, Forensic genetics, Serbian population

Corresponding author: Milica Keckarevic Markovic, Centre for Forensic and Applied Molecular Genetics, Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia, milica@bio.bg.ac.rs, phone: +381653376868

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Published in „GENETIKA“ Vol. 55, No. 2(2023), pp. 743-757

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2302743A
Original scientific article

ASSESSMENT OF COMBINING ABILITY COMPRISING alc, nor AND rin MUTANT ALLELES OF TOMATO UNDER MAIN AND LATE PLANTING SEASONS

S. D.AEER, S. K. JINDAL*, S. A. H. PATEL, N. CHAWLA

Department of Vegetable Science, Punjab Agricultural University,

Ludhiana, Punjab, India- 141 004.

Abstract

Aeer S. D., S. K.Jindal, S. A. H. Patel, N. Chawla (2023). Assessment of combining ability comprising alc, nor and rin mutant alleles of tomato under main and late planting seasons.- Genetika, Vol 55, No.2,743 -757.

Seventy four genotypes of the tomato (14 lines, four testers of ripening mutant and their 56 F₁ crosses developed in line Ч tester mating fashion) were appraised under main (E₁) and late (E₂) planting season at Department of Vegetable Science, Punjab Agricultural University, Ludhiana, Punjab, India. To find out few general and specific combiners, if some, which might be utilized in furthermore breeding programmes to prolong the fruit harvesting span of tomato in the Punjab conditions. The findings hinted in general, the particular parent or hybrid could not be used to assess all examined characters with same efficiency. In main (E₁) season line, FL-556 was found best general combiner for average fruit weight, locules number, pericarp thickness, P/E ratio, dry matter and lycopene content; PAU 114 for average fruit weight, lesser locules, pericarp thickness, TSS and dry matter. During late (E₂) planting season line, Punjab Ratta found with good GCA values for average fruit weight, lesser locules number, pericarp thickness and TSS content; Roma for average fruit weight, locules number, pericarp thickness and P/E ratio. Similarly tester, alc-IIHR-2050 for pollen viability, minimum days from transplanting to first harvest, harvesting span, average fruit weight, pericarp thickness and P/E ratio in main (E₁) season while, for pollen viability, average fruit weight, pericarp thickness and P/E ratio in late (E₂) season. The received results furthermore illustrated the best combinations that possessed significant values of SCA which effectively combine to develop a cross with good performance. In main (E₁) season from combinations, F₁ hybrid SMZ-867 Ч rin-Rutgers were registered with best specific combiner for pollen viability, average fruit weight, lesser locules number, Pericarp thickness and P/E ratio; PAU 2381 Ч nor-RM-1 for harvesting span, average fruit weight, pericarp thickness, total fruit yield and lycopene content. Likewise, in late (E₂) season cross, SMZ-867 Ч nor-RM-1 for pollen viability, minimum days from transplanting to first harvest, harvesting span and fruit yield; LT-44 Ч alc-IIHR-2050 for pollen viability, average fruit weight, lesser locules and pericarp thickness.

Keywords: combining ability, harvesting span, ripening mutants, testers, tomato

Corresponding author: Aeer S. D., S. K. Jindal, Department of Vegetable Science,Punjab Agricultural University, Ludhiana, Punjab, India- 141 004. E-mail: saleshjindal@pau.edu

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Published in „GENETIKA“ Vol. 55, No. 2(2023), pp. 759-773

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2302759E

Original scientific article

VARIABILITY AND CORRELATION BETWEEN PROPERTIES OF MAIZE HYBRID SEEDS OF YIELD AND QUALITY CHARACTERISTICS OF DURUM WHEAT GENOTYPES UNDER RAINFED CONDITIONS IN CENTRAL ANATOLIA REGION

Asuman KAPLAN EVLİCE^1*, Єule KESKİN², Aliye PEHLİVAN², Turgay ЄANAL²,

Ferda ЬNSAL CANAY², Ramazan AVCIOĞLU², Ayten SALANTUR³, Selami YAZAR³, Bayram ЦZDEMİR³, Mehmet Emin ALYAMAЗ³, Gцkhan KILIЗ³, Muhsin İbrahim AVCI³, Fatma Betьl SADE³

¹Sivas University of Science and Technology, Faculty of Agricultural Sciences and Technology, Department of Plant Production and Technologies, Sivas, Turkey

²Quality and Technology Department, Central Research Institute for Field Crops, Ankara, Turkey

³Breeding and Genetics Department, Central Research Institute for Field Crops, Ankara, Turkey

Abstract

Evlice Kaplan A., Є. Keskin, A. Pehlivan, T. Єanal, F. Ьnsal Canay, R. Avcıoğlu, A. Salantur, S. Yazar, B. Цzdemir, M. E. Alyamaз, G. Kılıз, M. İ. Avcı, F. B. Sade (2023). Yield and quality characteristics of durum wheat genotypes under rainfed conditions in Central Anatolia Region. - Genetika, Vol 55, No.2,759-773.

The study aimed to investigate the possibility of integrated assessment of durum wheat genotypes in multi-environmental trials for grain quality and yield. The most important selection parameters for durum wheat grain quality were analysed: kernel weight, test weight, kernel diameter, hardness index, kernel vitreousness, colour L, a, b values, grain protein content, SDS sedimentation, and modified SDS sedimentation. A wide variation was observed for all quality characteristics as well as grain yield. Variations in test weight and grain yield in different environments were significantly higher than those found among cultivars, whereas variations in hardness index, kernel vitreousness, colour L and b values, grain protein content, SDS sedimentation, and modified SDS sedimentation among cultivars were significantly higher than those found between environments. Variations in kernel weight, kernel diameter, and colour a value were similar for cultivars and environments. Heritability values of the traits in the study ranged from 0.28 for grain yield to 0.99 for the SDS sedimentation test. Significant relationships were observed among traits according to both biplot and correlation analyses. The İkizce location, where the highest mean grain yield and the lowest variation (347 kg/da, 299-412 kg/da, respectively) were obtained, might be considered the most suitable location for wheat production. Nine genotypes out of twenty-four evaluated showed good values for SDS sedimentation, colour value b, and grain yield and could be considered the most promising lines for selection.

Keywords: breeding, durum wheat, quality

Corresponding author: Asuman Kaplan Evlice, Sivas University of Science and Technology, Gьltepe Mah. Mecnun Otyakmaz Cd. No:1, 58100 Sivas – Tьrkiye, Phone: +90 346 219 1398, Fax: +90 346 219 1678, E-mail: asuman.kaplanevlice@sivas.edu.tr, aevlice@gmail.com

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Published in „GENETIKA“ Vol. 55, No2(2023), pp.775-784

Serbia

https://doi.org/10.2298/GENSR2302775A

TWO REVOLUTIONS: COPERNICUS AND DARWIN

Francisco J. AYALA

(1934-2023)

Inaugural lecture delivered on the 23^rd of May 2006

at the Serbian Academy of Sciences and Arts

Abstract

Ayala J. F. (2023): Two revolutions: Copernicus and Darwin. -- Genetika, Vol 55, No.2, 775-784.

Election to the distinguished Serbian Academy of Sciences and Arts is a very great honor. My election as a Foreign Member is, indeed, a great distinction that I shall henceforward treasure with both pride and humility. With pride, because of the eminent distinction of the Academy. With humility, because how could I possibly have deserved such recognition? I accept this honor not as an earned merit but as an undeserved and much valued gift.

This gift is particularly precious to me because of my love for the beautiful land of Serbia and its historical and cultural treasures, which I have appreciated and enjoyed on numerous visits that started more than a quarter of a century ago. Beyond the land and its riches, there is my scientific association and long-standing friendship with Serbian scientists.

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Published in „GENETIKA“ Vol. 55, No. 2(2023), pp. 785

Serbia

https://doi.org/10.2298/GENSR2302785E

CORRIGENDUM

Sneћana Mladenoviж Driniж, Editor of the journal Genetika

request from Alireza IRANBAKHSH to remove names Alireza IRANBAKHSH, Yones ASRI, Saeed Zaker BOSTANABAD from the paper

GENETIC DIVERSITY AND POPULATION STRUCTURE ANALYSIS IN Lonicera L. (Caprifoliaceae) WITH THE USE OF ISSR MOLECULAR MARKERS

Shima GARSHASBI1, Alireza IRANBAKHSH1, Yones ASRI2, Saeed Zaker BOSTANABAD3

1Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran

3Department of Microbiology and Biology, Parand branch, Islamic Azad University, Tehran, Iran

Original scientific paper

https://doi.org/10.2298/GENSR2103273G

published in the journal Genetika, 2021, Vol 53, No.3, 1273 – 1286

due to misuse of the email address of Alireza IRANBAKHSH by the first author Shima Garshasbi. The first authors of the article are responsible for writing, submitting, and other unethical behaviors.

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Published in „GENETIKA“ Vol. 55, No. 2(2023), pp 786

Serbia

https://doi.org/10.2298/GENSR2302787E

CORRIGENDUM

Sneћana Mladenoviж Driniж, Editor of the journal Genetika

request from Alireza IRANBAKHSH to remove names Alireza IRANBAKHSH, Mostafa ASSADI, Silvio FICI from the paper

SPECIES DELIMITATION IN CAPPARIS (CAPPARACEAE): MORPHOLOGICAL AND MOLECULAR

Sakine NAJAFIAN1, Iraj MEHREGAN1, Alireza IRANBAKHSH1, Mostafa ASSADI2, Silvio FICI3

1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Research Institute of Forest and Rangelands, National Botanical Garden of Iran, Tehran, Iran

3 Dipartmento di Science Agrarie e Forestali, University di Palermo, Palermo, Italy

Original scientific paper

https://doi.org/10.2298/GENSR2102609N

published in the journal Genetika, 2021, Vol 53, No.2, 609 – 627

due to misuse of the email address of Alireza IRANBAKHSH by the first author Sakine NAJAFIAN. The first authors of the article are responsible for writing, submitting, and other unethical behaviors.

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Published in „GENETIKA“ Vol. 55, No.2(2023), pp. 787

https://doi.org/10.2298/GENSR2302789E

CORRIGENDUM

Sneћana Mladenoviж Driniж, Editor of the journal Genetika

request from Iraj Mehregan to remove name from the paper

SPECIES DELIMITATION IN CAPPARIS (CAPPARACEAE): MORPHOLOGICAL AND MOLECULAR

Sakine NAJAFIAN1, Iraj MEHREGAN1, Alireza IRANBAKHSH1, Mostafa ASSADI2, Silvio FICI3

1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Research Institute of Forest and Rangelands, National Botanical Garden of Iran, Tehran, Iran

3 Dipartmento di Science Agrarie e Forestali, University di Palermo, Palermo, Italy

Original scientific paper

https://doi.org/10.2298/GENSR2102609N

published in the journal Genetika, 2021, Vol 53, No.2, 609 – 627

The first author Sakine NAJAFIAN of the article are responsible for writing, submitting, and other unethical behaviors.

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Published in „GENETIKA“ Vol. 55, No.2(2023), pp. 791-793

Serbia

https://doi.org/10.2298/GENSR2302791E

RETRACTION NOTICE

Editor

The article listed below, published in journal Genetika has been retracted due to evidence indicating that the peer review of this paper was compromised, using of frauted data, high number of unfitting citation, overoll general misconduct related to professional codes of ethics. All papers which belong to this group have passed a regular review process. As part of the reviewing process, according to Journal policy, it is expected from reviewers to check all relevant data including citations probity. All papers were published after two positive reviewers’ opinions. The journal Genetika condemns such an unethical behavior and will take all necessary measures to ensure that such incidents do not happen again in the future.

Authors of those papers as well reviewers are barred from publishing in the journal Genetika in the future and will be blacklisted by the journal.

The list of retracted articles is:

1. Bouzarisaravani Z., F. Sharifnia, F. Salimpour, S. Arbabian, A. Geran (2021). Molecular systematic studies in the genus Glaucium (Papaveraceae). - Genetika, Vol 53, No.3, 1179-1192 https://doi.org/10.2298/GENSR2103179B

2. Hang L., L. Pan, T. Yong, L. Jianguo, X. Xingmin, Faisal (2021). Population genetic structure and gene flow in Alcea aucheri (boiss.) Alef.: a potential medicinal plant- Genetika, Vol 53, No.2, 867-882. https://doi.org/10.2298/GENSR2102867H

3. Jiao L., H. Xiao, X. Zhao, F. M. Abarghuei (2021). RAPD profiling in detecting genetic variation in Glaucium (Papaveraceae) species: Edible and Medicinal plant. - Genetika, Vol 53, No.3,1081 - 1092. https://doi.org/10.2298/GENSR2103081J

4. Li H., H. Yu, X. Zeng, S. Hussein Hamarashid (2021). Study on genetic diversity between Malva L. (Malvaceae): a high value medicinal plant using SCoT molecular markers.- Genetika, Vol 53, No.2, 895-910 https://doi.org/10.2298/GENSR2102895L

5. Li H., Y. Wang, R, Iqbal (2021). SCoT molecular markers and population differentiation in Hedera helix L.. - Genetika, Vol 53, No.2, 739-756. https://doi.org/10.2298/GENSR2102739L

6. Li J., X. Yang, S.Mehri (2021). Genetic diversity in Stellaria L. (Caryophyllaceae) using sequence related amplified polymorphism. - Genetika, Vol 53, No.3, 1369 - 1377. https://doi.org/10.2298/GENSR2103369L

7. Lin L., L. Lin, A.Waheed (2021). Assessment of genetic structure and diversity of Erodium (Geranaiceae) species. - Genetika, Vol 53, No.2, 507-520. https://doi.org/10.2298/GENSR2102507L

8. Li S. X. Jiang, S. Mehri (2021). Genetic diversity and gene-pool of Aegilops tauschii coss. (Poaceae) based on retrotransposon-based markers. - Genetika, Vol 53, No.3, 1331- 1340.

https://doi.org/10.2298/GENSR2103331L

9. Ma X., H. Tian, H. Xia, Zeenat (2021). Genetic diversity of Lonicera L. (caprifoliaceae) estimated by molecular markers and morphological characters. - Genetika, Vol 53, No.2, 651-662. https://doi.org/10.2298/GENSR2102651M

10. Mahdavi M., F. Sharifnia, F.Salimpour, A. Esmaeili, M. Larypoor (2021). Genetic diversity and population structure of Iranian pistachio (Pistacia vera L.) cultivars.- Genetika, Vol 53, No.2, 671-686 https://doi.org/10.2298/GENSR2102671M

11. Meng K., J. Yao, C.Y. He and H. Morabbi Heravi (2021). Gene flow and genetic structure between populations of Hesperis L. (Brassicaceae) species using molecular markers. - Genetika, Vol 53, No.2, 769-782. https://doi.org/10.2298/GENSR2102769M

12. Mowang S.-C., F.-J. Chen, Zeenat (2021). Study on genetic diversity between Erodium (Geranaiceae) species based on inter-simple sequence repeat markers- Genetika, Vol 53, No.2, 927-939. https://doi.org/10.2298/GENSR2102837M

13. Najafian S., I.Mehregan, A. Iranbakhsh, M. Assadi, S. Fici (2021). Species delimitation in Capparis (Capparaceae): morphological and molecular. - Genetika, Vol 53, No.2, 609-627. https://doi.org/10.2298/GENSR2102609N

name mark red not autors of paper (corrigentdum)

14. Nikkhah M., S. Arbabian, A. Majd, F. Sharifnia (2022). Genetic diversity of Cordia myxa L. assessed by ISSR markers. - Genetika, Vol 54, No.1, 63-72. https://doi.org/10.2298/GENSR2201063N

15. Ou C., Z. Shen, Y. Liu, Z. Wang, M. Farshadfar (2021). Morphometric analysis and genetic diversity in Pistacia species populations using sequence related amplified polymorphism. - Genetika, Vol 53, No.3, 1193-1205 https://doi.org/10.2298/GENSR2103193O

16. Qian X. and S. Mehri (2021). Detecting DNA polymorphism and genetic diversity in a wide pistachio germplasm by RAPD markers- Genetika, Vol 53, No.2, 783-798 https://doi.org/10.2298/GENSR2102783Q

17. Xu P.,C. Xu, X.Huang, H.Wang, H. Morabbi Heravi (2021). Genetic diversity and genepool of Salicornia sinus-persica akhani based on retrotransposon-based markers. - Genetika, Vol 53, No.3, 1287 - 1296.https://doi.org/10.2298/GENSR2103287X

18. Garshasbi S., A. Iranbakhsh, Y. Asri, S. Z. Bostanabad (2021). Genetic diversity and population structure analysis in Lonicera L. (Caprifoliaceae) with the use of ISSR molecular markers. - Genetika, Vol 53, No.3, 1273 – 1286 https://doi.org/10.2298/GENSR2103273G

name mark red not autors of paper (corrigentdum)

19. Sun Y., H. Jiang, F. Zeng, X. Pan, X. Wu, Y. Qi, X. Wu (2022). Species identification and genetic diversity of Alcea (Malvaceae) using SCOT molecular markers: medicinal plant. - Genetika, Vol 54, No.1, 369-378. https://doi.org/10.2298/GENSR2201369S

20. Ting S. and Y. Yibing (2022). Population differentiation and gene flow of Glaucium flavum (Papaveraceae). - Genetika, Vol 54, No.1, 275-288 https://doi.org/10.2298/GENSR2201275T

21. Xu P.,C. Xu, X.Huang, H.Wang, H. Morabbi Heravi (2021). Genetic diversity and genepool of Salicornia sinus-persica akhani based on retrotransposon-based markers. - Genetika, Vol 53, No.3, 1287 - 1296. https://doi.org/10.2298/GENSR2103287X

22. Yanpeng Z., W. Hongmei, L. Wei, M. Khayatnezhad, Faisal (2021). Genetic diversity and relationships among Salvia species by ISSR markers. - Genetika, Vol 53, No.2, 559-574. https://doi.org/10.2298/GENSR2102559Y

23. Yao X., R. Zhou, M.Farshadfar (2021). Comparison of individual based approaches using RAPD markers for identifying genetic relationships in Erodium (Geranaiceae)- Genetika, Vol 53, No.3, 1229 - 1238 https://doi.org/10.2298/GENSR2103229Y

24. Yin J. (2022). Evaluation of genetic variability Rindera using RAPD markers. - Genetika, Vol 54, No.1, 173-186. https://doi.org/10.2298/GENSR2201173Y

25. Zhang X. and A. Shakoor (2021). Strong genetic differentiation of the Paracaryum species (Boraginaceae) detected by inter-simple sequence repeats (ISSR).- Genetika, Vol 53, No.2, 883-894 https://doi.org/10.2298/GENSR2102883Z

26. Zhang Z., H. Yu, S. Feng, A. A. Minaeifar (2021). Species identification and population structure analysis in Hesperis L. (Brassicaceae). - Genetika, Vol 53, No.3, 1357 - 1368 https://doi.org/10.2298/GENSR2103357Z

27. Zhou Y. and Z. Zheng (2022). Genetic Diversity and inter-relationship among Stellaria L. (Caryophyllaceae) species ISSR markers. - Genetika, Vol 54, No.1, 119-130. https://doi.org/10.2298/GENSR2201119Z

In addition, Clarivate provided the publisher with evidence of inappropriate manipulation of citations of five paper published in journal Genetika in journal Bioscenece research:

Bi D., D. Chen, M. Khayatnezhad, Z. S. Hashjin, Z. Li, Y. Ma (2021). Genetic response of growth phases for abiotic environmental stress tolerance in cereal crop plants. - Genetika, Vol 53, No.1,393-405 https://doi.org/10.2298/GENSR2101393B
Chen W., M. Khayatnezhad, N, Sarhadi (2021). Gene flow and population structure in Allochrusa (Caryophylloideae, caryophyllaceae) with the use of molecular markers- Genetika, Vol 53, No.2, 799-812 https://doi.org/10.2298/GENSR2102799C
Jia Y., M. Khayatnezhad, S. Mehri (2020). Population differentiation and gene flow in Erodium cicutarium: a potential medicinal plant- Genetika, Vol 52, No.3, 1127-1144. https://doi.org/10.2298/GENSR2003127J
Peng X., M. Khayyatnezhad and L. Joudi Ghezeljehmeidan (2021). RAPD profiling in detecting genetic variation in Stellaria L. (Caryophyllaceae).- Genetika, Vol 53, No.1,349 -362. https://doi.org/10.2298/GENSR2101349P
Yin J., M. Khayatnezhad, A. Shakoor (2021). Evaluation of genetic diversity in geranium (Geraniaceae) using RAPD marker.- Genetika, Vol 53, No.1,363 -378. https://doi.org/10.2298/GENSR2101363Y

Authors who misused the papers published in Genetika by citing them unjustifiably as well as authors of the cited papers are barred from publishing in that journal in the future and will be blacklisted by the journal.

We would like to apologize authors, readers and all scientific community that we are having to make those retractions, and we will take all necessary steps to ensure our editorial and peer review processes keep pace with the evolving threat and advancements in scientific fraud.

Sneћana Mladenoviж Driniж

editor

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