GENETIKA, Vol. 53, No.3 (2021)
Bojana PETROVIĆ and Milica KOMNENIĆ RADOVANOVIĆ
CHROMOSOMAL ANALYSIS IN 110 PATIENTS WITH PRIMARY AMENORRHEA [Abstract] [Full text]
Sneha ADHIKARI, Anjali JOSHI, Amarjeet KUMAR, Narendra Kumar SINGH, Jai Prakash JAISWAL, Anand Singh JEENA, Usha PANT
IDENTIFICATION OF QTLS FOR YIELD AND CONTRIBUTING TRAITS IN MAIZE-TEOSINTE DERIVED BILS UNDER DISEASED-STRESSED AND CONTROL CONDITIONS [Abstract] [Full text]
Krasimira TANEVA, Violeta BOZHANOVA, Elena TODOROVSKA
STUDY OF CAROTENOID CONTENT IN DURUM WHEAT [Abstract] [Full
text]
Dragana JUGOVIĆ, Perica
VASILJEVIĆ, Ljiljana BRANKOVIĆ, Radovan MILIĆEVIĆ, Višnja MADIĆ, arija
VUKELIĆ-NIKOLIĆ
FREQUENCY
OF NRAS GENE MUTATIONS AMONG THE PATIENTS WITH WILD TYPE KRAS COLORECTAL
CANCERS IN SOUTHERN-EASTERN SERBIA [Abstract] [Full
text]
Seyed Mehdi HASHEMI, Atena FARSI, Gholamreza BAHARI, Hoseinali DANESH, Kamran ROUDINI
EVALUATION OF 40-bp DELETION / INSERTION POLYMORPHISM OF mdm2 AND 16-bp DELETION / INSERTION POLYMORPHISM OF p53 GENE IN PATIENTS WITH LYMPHOMA IN A PERSIAN POPULATION [Abstract] [Full text]
Jelena STAMENKOVIĆ, Bojana PETROVIĆ, Nela MAKSIMOVIĆ, Jovana JOKSIMOVIĆ, Luka JOKSIMOVIĆ, Olivera KONTIĆ-VUČINIĆ
ASSOCIATION BETWEEN ISOLATED MILD SYMMETRICAL
VENTRICULOMEGALY AND FETAL CHROMOSOMAL ABERRATIONS [Abstract] [Full
text]
Rutaba GUL, Sabika FIRASAT, Mulazim HUSSAIN, Muhammad TUFAIL, Waheed AHMAD, Kiran AFSHAN
NEUROLOGICAL MANIFESTATIONS IN PAKISTANI LYSOSOMAL STORAGE DISORDERS PATIENTS AND MOLECULAR CHARACTERIZATION OF GAUCHER DISEASE [Abstract] [Full text]
Valentin KOSEV and Viliana VASILEVA
Correlation dependences on quantitive signs in grass pea (Lathyrus sativus L.) accessions [Abstract] [Full text]
Kazım MAVİ, Kazim GÜNDÜZ, Fulya UZUNOĞLU, Fırat Ege KARAAT
MORPHOLOGICAL CHARACTERIZATION OF SPONGE GOURD (Luffa
aegyptiaca Mill.) GENOTYPES FROM THE EASTERN MEDITERRANEAN REGION OF TURKEY [Abstract] [Full
text]
Madiha AMJAD, Qaisar AKRAM, Humara KAUSER, Azam ALI, Qurban ALI
MOLECULAR BASED IDENTIFICATION OF Tem ß-lactamase AND Tet A RESISTANCE GENE IN E. coli [Abstract] [Full
text]
Lei JIAO, HuaPing XIAO² Xu ZHAO, Faezeh Mirazimi ABARGHUEI
RAPD PROFILING IN DETECTING GENETIC VARIATION IN Glaucium (Papaveraceae) SPECIES: EDIBLE AND MEDICINAL PLANT [Abstract] [Full text] [Supplement]
Faiza TAWAB, Afaq AHMAD, Adnan NASIM, Saleha TAWAB, Iqbal MUNIR
SALT STRESS TOLERANCE POTENTIAL OF SELECTED RICE CULTIVARS OF PAKISTAN [Abstract] [Full text]
Radmila BESKOROVAJNI, Rade JOVANOVIĆ, Lato PEZO, Nikola POPOVIĆ, Nataša TOLIMIR, Ljubiša MIHAJLOVIĆ, Gordana ŠURLAN-MOMIROVIĆ
MATHEMATICAL MODELING FOR GENOMIC SELECTION IN SERBIAN DAIRY CATTLE[Abstract] [Full text]
Sonja TANČIĆ ŽIVANOV, Boško DEDIĆ, Sandra CVEJIĆ, Siniša JOCIĆ, Vladimir MIKLIČ
SUNFLOWER GENOTYPES TOLERANCE TO CHARCOAL ROT (Macrophomina phaseolina (TASSI) GOID.) UNDER THE FIELD CONDITIONS [Abstract] [Full text]
ASSESSMENT OF FRUIT FLESH BROWNING IN ‘AMASYA’ APPLE CULTIVAR AND ITS TWO SELECTED SIBS [Abstract] [Full text]
Manja BOŽIĆ, Aleksandra STANOJEVIĆ, Ksenija MARKOVIĆ, Dragana IGNJATOVIĆ MICIĆ, Dragana NIKOLIĆ, Marija MILIVOJEVIĆ, Ana NIKOLIĆ
PHYSIOLOGICAL AND GENE EXPRESSION CHANGES
DURING IMBIBITION IN MAIZE SEEDS UNDER LOW TEMPERATURE CONDITIONS [Abstract] [Full
text]
Fahimeh ZANGANEH and Masoud SHEIDAI
ASSOCIATION OF RS1294845152 IN CRISP2 WITH MALE INFERTILITY: RFLP AND DNA SEQUENCE ANALYSIS [Abstract] [Full text]
Zahra BOUZARISARAVANI, Fariba SHARIFNIA, Fahimeh SALIMPOUR, Sedigheh ARBABIAN, Afsaneh GERAN
MOLECULAR SYSTEMATIC STUDIES IN THE GENUS Glaucium (Papaveraceae) [Abstract] [Full text]
Chun OU, Zhongyuan SHEN, Yu LIU, Zelu WANG and Mohsen FARSHADFAR
MORPHOMETRIC ANALYSIS AND GENETIC DIVERSITY IN Pistacia SPECIES POPULATIONS USING SEQUENCE RELATED AMPLIFIED POLYMORPHISM [Abstract] [Full text]
Kenneth C. ONYEGBULA, Benjamin O. EMIKPE, Akinyele O. ADISA, Chiaka I. ANUMUDU
RUNX3 PROMOTER METHYLATION IS ASSOCIATED WITH ORAL SQUAMOUS CELL CARCINOMA LOCATION [Abstract] [Full text]
Sanja PERIĆ, Milan STEVANOVIĆ, Slaven PRODANOVIĆ, Snežana MLADENOVIĆ DRINIĆ, Nikola GRČIĆ, Vesna KANDIĆ, Jovan PAVLOV
GENETIC DISTANCE OF MAIZE INBREDS FOR PREDICTION OF HETEROSIS AND COMBINING ABILITY [Abstract] [Full text]
Xixi YAO, Rui ZHOU, Mohsen FARSHADFAR
COMPARISON OF INDIVIDUAL BASED APPROACHES USING RAPD MARKERS FOR IDENTIFYING GENETIC RELATIONSHIPS IN Erodium (Geranaiceae) [Abstract] [Full text]
Maria KOMAL, Kiran AFSHAN, Seemi ZAFAR, Muhammad Asim KHAN, Sabika FIRASAT, Mazhar QAYYUM
RAPID IMMUNODETECTION ASSAY BASED ON SOMATIC AND EXCRETORY SECRETORY ANTIGEN OF Fasciola SPECIES IN LARGE RUMINANTS [Abstract] [Full text]
Firdes ULAS, Saliha ERDOGDU, Halit YETISIR, Abdullah ULAS
Investigation on Morphology and Physiology of Nitrogen Efficiency in Different Pepper (Capsicum annuum L.) Inbred Lines [Abstract] [Full text]
Shima GARSHASBI, Alireza IRANBAKHSH, Yones ASRI, Saeed Zaker BOSTANABAD
GENETIC DIVERSITY AND POPULATION STRUCTURE ANALYSIS IN Lonicera L. (Caprifoliaceae) WITH THE USE OF ISSR MOLECULAR MARKERS [Abstract] [Full text]
Pengyuan XU, Chenheng XU, Xuan HUANG, Hua WANG, Hakimeh MORABBI HERAVI
GENETIC DIVERSITY AND GENE-POOL OF Salicornia sinus-persica Akhani BASED ON RETROTRANSPOSON-BASED MARKERS [Abstract] [Full text]
K.R.UTHAYAN, S.MOHANAVALLI, B.NIVETHA, S. DHIVYA
OPTIMISED FEATURE SELECTION FOR EARLY CANCER DETECTION [Abstract] [Full text]
Milica NIKOLIĆ, Jelena SRDIĆ, Iva SAVIĆ, Slađana ŽILIĆ, Milan STEVANOVIĆ, Vesna KANDIĆ, Slavica STANKOVIĆ
The occurrence of mycotoxins in sweet maize hybrids [Abstract] [Full text]
Kuanhong MENG, Jia YAO, CongYing HEand Mohsen FARSHADFAR
INTER-SIMPLE SEQUENCE REPEATS GENETIC RELATIONSHIPS BETWEEN POPULATIONS OF Consolida (RANUNCULACEAE) [Abstract] [Full text]
Sha LI, Xiaomei JIANG, Shahram MEHRI
GENETIC DIVERSITY AND GENE-POOL OF Aegilops tauschii Coss. (Poaceae) BASED ON RETROTRANSPOSON-BASED MARKERS [Abstract] [Full text]
PROTEIN QUALITY PARAMETERS AND STORAGE PROTEIN PROFILING OF MUNGBEAN INTERSPECIFIC LINES (Vigna radiata L. Wilczek) [Abstract] [Full text] [Supplement]
Zhiwei ZHANG, Hangcheng YU, Shuowen FENG, Amir Abbas MINAEIFAR
SPECIES IDENTIFICATION AND POPULATION STRUCTURE ANALYSIS IN Hesperis L. (Brassicaceae) [Abstract] [Full text]
Jialing LI , Xin YANG, Shahram MEHRI
GENETIC DIVERSITY IN Stellaria L. (Caryophyllaceae) USING SEQUENCE RELATED AMPLIFIED POLYMORPHISM [Abstract] [Full text]
Azam ALI, Nazim HUSSAIN, Muhammad Saqib SHAHZAD, INAMULLAH, Qurban ALI
X CHROMOSOMAL ANALYSIS IN POPULATION GENETICS AND FORENSIC SCIENCE: A MINI REVIEW [Abstract] [Full text]
Editor
CORRIGENDUM Yaocheng JIA, Majid KHAYYATNEZHAD, Shahram MEHRI [Abstract] [Full text]
Editor
CORRIGENDUM Xiaobang PENG, Majid KHAYYATNEZHAD and Leila JOUDI GHEZELJEHMEIDAN [Abstract] [Full text]
Editor
CORRIGENDUM Xiaobang PENG, Majid KHAYYATNEZHAD and Leila JOUDI GHEZELJEHMEIDAN [Abstract] [Full text]
Editor
CORRIGENDUM Xiaobang PENG, Majid KHAYYATNEZHAD and Leila JOUDI GHEZELJEHMEIDAN [Abstract] [Full text]
Editor
CORRIGENDUM Xiaobang PENG, Majid KHAYYATNEZHAD and Leila JOUDI GHEZELJEHMEIDAN [Abstract] [Full text]
Published in „GENETIKA“ Vol. 53, No.3(2021), pp. 943-950
© 2021 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
UDC 575.
https://doi.org/10.2298/GENSR2103943P
Orginal
scientific article
CHROMOSOMAL ANALYSIS IN 110 PATIENTS WITH PRIMARY AMENORRHEA
Bojana PETROVIĆ and Milica KOMNENIĆ RADOVANOVIĆ
Clinic for gynecology and obstetrics, Clinical center of Serbia, Belgrade, Serbia
Abstract
Petrović B. and M. Komnenić Radovanović (2021). Chromosomal analysis in 110 patients with primary amenorrhea. - Genetika, Vol 53, No.3, 943- 950.
Objects: This study was undertaken to investigate frequency and the type of chromosomal aberrations which are causing primary amenorrhea in our patients. Methods: The study subjects included 110 patients referred with primary amenorrhea for cytogenetics investigation and counseling. Primary amenorrhea was defined as the absence of menstruation and secondary sexual characteristics in phenotypic women aged 14 years or older, or aged 16 years or older if secondary sexual characteristics were present. Peripheral blood samples were processed using standard techniques. All spacimens were G-banded using tripsin-Giemsa. For each case, 22 metaphase spreads were analyzed and when mosaicism was suspect 100 metaphases were examined. Results: We have examined cytogenetically 110 patients with primary amenorrhea. Chromosomal aberrations were detected in 21 cases (19,1%). Male karyotype (46,XY) was found in seven cases, as well as monosomy X (45,X). In three cases isochromosome X (46,XiXq) was detected. We found two cases of mosaic karyotype 46,XX/45,X and X chromosome trisomy (47,XXX) and mosaic karyotype 45,X/46,XiXq in one case each. Conclusion: Chromosome aberrations are one of the main causes of primary amenorrhea. The search for genetic component is the utmost importance for diagnosis, risk assessment and genetic counseling.
Key words: primary amenorrhea, chromosome aberrations, X chromosome aneuploidies
Corresponding author: Bojana Petrović, Clinic for gynecology and obstetrics, Clinical center of Serbia, Belgrade, Serbia, e-mail: mrdrbojaninmail@gmail.com
Published in „GENETIKA“ Vol. 53, No. 3 (2021), pp.951-972
© 2021Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC
575.633
https://doi.org/10.2298/GENSR2103951A
Original
scientific paper
IDENTIFICATION OF QTLS FOR YIELD AND CONTRIBUTING TRAITS IN MAIZE-TEOSINTE DERIVED BILS UNDER DISEASED-STRESSED AND CONTROL CONDITIONS
Sneha ADHIKARI*1,2, Anjali JOSHI1,3, Amarjeet KUMAR1,4, Narendra Kumar SINGH1,
Jai Prakash JAISWAL1, Anand Singh JEENA1, Usha PANT1
1Department of Genetics and Plant Breeding, G. B. P. U. A. & T. Pantnagar, U. S. Nagar, Uttarakhand -263145, India
2ICAR-Indian Institute of Wheat and Barley Research, Regional Station Flowerdale, Shimla, H.P., 171002, India
3Genetics and Tree Improvement Division, Arid Forest Research Institute, Jodhpur, Rajasthan-342005, India
4Department of Genetics and Plant Breeding, College of Horticulture, Thenzawl, CAU, Imphal, India
Abstract
Adhikari S., A. Joshi, A. Kumar, N. Kumar Singh, J. Prakash Jaiswal, A. Singh Jeena, U. Pant (2021). Identification of QTLS for yield and contributing traits in maize-teosinte derived bils under diseased-stressed and control conditions. - Genetika, Vol 53, No.3, 951-972.
In maize, grain yield is the most important trait having a complex inheritance pattern. Yield contributing traits are more stable and have higher heritability than yield. Therefore, the present study was conducted to identify quantitative trait loci (QTLs) associated with grain yield and its components by using simple sequence repeat (SSR) markers. A population of 169 BC1F5 lines was derived from the crossing between maize inbred line DI-103 and teosinte-parviglumis was utilized for genotyping and phenotyping. In diseased stressed condition (E1), ear length (EL), ear diameter (ED), kernel rows per ear (KR/E), kernels per row (K/R), test weight (TW), and grain yield per plant (GY/P) had 7, 6, 7, 4, 6 and 5 QTLs whereas, in controlled condition (E2) 5, 2, 5, 4, 5 and 3 QTLs were detected for enlisted characters, respectively. Consistent QTLs across the environments were detected for 5 of the 6 investigated traits and number of QTLs were EL (2), ED (1), KR/E (3), TW (1), and GY/P (1) whereas, for K/R none of the QTLs were common between E1 and E2. By mapping analysis, we have identified genomic regions associated with two traits in a manner that was consistent with phenotypic correlations among traits, supporting either pleiotropy or tight linkage among QTLs. Three co-localized QTLs were identified between grain yield and contributing traits. Notably umc1720-linked QTL at bin 4.10 was simultaneously responsible for GY and EL, ED, KR/E, K/R; umc1215-linked QTL at bin 6.03 was simultaneously responsible for GY and ED, KR/E, K/R, TW; umc1279-linked QTL was responsible for GY and ED, TW. The findings suggest that the chromosomal region containing co-localized QTLs governing multiple yields associated traits are potential targets for selection. In addition for 6 studied traits, 44 superior lines were identified, and along with both the parents i.e. maize (DI-103) and teosinte they were clustered in 11 groups. Therefore, lines clustered independently can be utilized in a hybridization programme for the accumulation of yield contributing traits for yield maximization.
Key words: Maize, Teosinte, QTL, SSR, Genotyping
Corresponding author: Sneha Adhikari, Department of Genetics and Plant Breeding, G. B. P. U. A. & T. Pantnagar, U. S. Nagar, Uttarakhand -263145, India, Email: snehaadhikari24@gmail.com, Phone number: 9917823448
Published in „GENETIKA“ Vol. 53, No.3 (2021), pp.973-983
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.11
https://doi.org/10.2298/GENSR2103973T
Original
scientific article
STUDY OF CAROTENOID CONTENT IN DURUM WHEAT
Krasimira TANEVA1*, Violeta BOZHANOVA1, Elena TODOROVSKA2
1Agricultural Academy, Field Crops Institute, Chirpan, Bulgaria
2Agricultural Academy, Agrobioinstitute, Sofia, Bulgaria
Abstract
Taneva K., V. Bozhanova, E. Todorovska (2021). Study of carotenoid content in durum wheat. - Genetika, Vol 53, No.3, 973-983.
The purpose of the study is to determine the variability and stability of traits related to the amount of yellow pigments in the grain, semolina and the finished pasta product. In addition the polymorphism in one of the phytoene synthase gene on chromosome 7A related to carotenoids synthesis was detected. In the investigation were included 10 durum wheat genotypes – cultivars and breeding lines of different origin – Bulgaria - Field Crops Institute – Chirpan and Dobroudja Agricultural Institute – General Toshevo, Europe, CYMIT-Mexico. All genotypes were grown in field conditions in competitive variety trials in three replications in harvesting years 2014/2016. The results obtained were processed statistically via basic statistics (TIBCO statistica 13.3.0 software package). Great variability was found between genotypes on the study traits. The breeding lines: M-431 (10.71 ppm in grain, 10.42 ppm in semolina and 8.96 ppm in pasta) and TD-97 (9.83 ppm in grain, 9.58 ppm in semolina and 8.52 ppm in pasta) and the Bulgarian standard cultivar Predel (9.25 ppm in grain, 8.87 ppm in semolina and 7.27 ppm in pasta) are characterized by the highest yellow pigment content in grain, semolina and pasta. Both lines are also distinguished by the lowest total pigment losses (2.6%) in milling grain to semolina and in processing to pasta product (11 – 11.4 %), while line TD-97 has the lowest total pigment losses (13.6%). Furthermore the genotypes characterized by the highest yellow pigment content (M-431 and TD-97) have an acceptable stability and can be used in the hybridization program to improve the color potential of Bulgarian durum wheat varieties. The Psy-A1 codominant marker, based on a 37 bp insertion at the 5' end of intron 2 of the PsyA1 gene amplifies two fragments. The Psy-A1a allele with a 194 bp fragment in length was observed in all 7 studied cultivars and lines of durum wheat. The 231-bp Psy-A1b allele is found in 3 genotypes. The obtained results could not be associated with phenotypic differences in the yellow pigment content in the durum wheat genotypes studied.
Key words: durum wheat, yellow pigments, phytoene synthase
Corresponding author: Krasimira Taneva, Agricultural Academy, Field Crops Institute, Chirpan, Bulgaria, email: krasimira.taneva@abv.bg
Published in „GENETIKA“ Vol. 53, No3(2021), pp. 985-996
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2103985J
Original
scientific article
FREQUENCY OF NRAS GENE MUTATIONS AMONG THE PATIENTS WITH WILD TYPE KRAS COLORECTAL CANCERS IN SOUTHERN-EASTERN SERBIA
Dragana JUGOVIĆ1*, Perica VASILJEVIĆ2, Ljiljana BRANKOVIĆ1, Radovan MILIĆEVIĆ1, Višnja MADIĆ2, Marija VUKELIĆ-NIKOLIĆ3
1 Laboratory for Immunology and Genetics, Center for Medical and Clinical biochemistry,
University Clinical Center, Niš, Serbia
2 University of Niš, Faculty of Science and Mathematics, Department of Biology and Ecology, Niš, Serbia
3 University of Niš, Faculty of Medicine, Research Center for Biomedicine, Niš, Serbia
Abstract
Jugović D., P. Vasiljević, Lj. Branković, R. Milićević, V. Madić, M. Vukelić-Nikolić (2021). Frequency of NRAS gene mutations among the patients with wild type Kras colorectal cancers in southern-eastern Serbia. - Genetika, Vol 53, No.3, 985-996.
Colorectal cancer (CRC) is the fourth most frequently diagnosed cancer worldwide, with 1.1 million cases in 2018. KRAS and NRAS genes are some of the most important molecular biomarkers of CRC. That is why, before starting treatment with anti-epidermal growth factor therapy, patients with CRC are tested for the mutation in those genes. The aim of this study was to evaluate the frequency of NRAS gene mutations among patients with wild type (wt) KRAS colorectal cancer in Southern-Eastern Serbia. Formalin-fixed paraffin-embedded sample tissues of 55 CRC patients with wt KRAS were investigated during the period from 2017 to 2019. Following DNA extraction, the samples were analysed for common mutations of exons 2 (codons 12 and 13), 3 (codon 61), and 4 (codon 117 and 146) of the NRAS gene using two diagnostic analyses: real-time PCR and NRAS StripAssay. Among these 55 cases of colorectal cancer with wt KRAS, there were 3 (5.4%) cases with mutant NRAS. One of these patients had mutations in codon 13 and the other two in codon 61. No mutation in codon 12 was found. Moreover, two out of three patients were men with CRC in the T3 stage of tumour infiltration and liver metastases. The third one was a woman with CRC in the T3 stage of tumour infiltration and lung metastases. Our results showed that the frequency of NRAS mutation in CRC is low, which is similar to other studies covering different geographic areas of the world.
Keywords: NRAS gene, KRAS gene, colorectal cancer
Corresponding author: Dragana Jugović, Laboratory for Immunology and Genetics, Center for Medical and Clinical biochemistry, Clinical Center of Niš, Bulevar Dr Zorana Đinđića 48, 18000 Niš, Serbia, Tel.: +381 603450233, Email address: dragana.jugovic@live.com
Published in „GENETIKA“ Vol. 53, No.3 (2021), pp. 997-1005
© 2021Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2103997H
Original
scientific paper
EVALUATION OF 40-bp DELETION / INSERTION POLYMORPHISM OF mdm2
AND 16-bp DELETION / INSERTION POLYMORPHISM OF p53 GENE IN PATIENTS WITH LYMPHOMA IN A PERSIAN POPULATION
Seyed Mehdi HASHEMI1, Atena FARSI2, Gholamreza BAHARI3, Hoseinali DANESH4, Kamran ROUDINI5*
1Associate Professor of Hematology & Oncology, Department of Internal Medicine, School of Medicine, Clinical Immunology Research Center, Ali Ibne Abitaleb Hospital, Zahedan University of Medical Sciences, Zahedan, Iran
2GP, Zahedan University of Medical Sciences, Zahedan, Iran
3Associate Professor of Clinical Biochemistry, Department of Biochemistry, School of Medicine, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran
4Plastic, Reconstructive & Aesthetic surgeon, Assistant professor of Zahedan University of Medical Sciences, Zahedan, Iran
5 Assistant Professor of Hematology & Oncology, Department of Internal Medicine, School of Medicine, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran
Abstract
Hashemi M. S., A. Farsi, G. Bahari, H. Danesh, K. Roudini (2021). Evaluation of 40-bp deletion / insertion polymorphism of mdm2 and 16-bp deletion / insertion polymorphism of p53 gene in patients with lymphoma in a Persian population. - Genetika, Vol 53, No.3, 997-1005.
Considering the importance of lymphoma and its prevalence in communities as well as its relationship with genetic factors (P53 & MDM2) as well as contradictory results about the possible role of deletion / insertion of polymorphisms in different types of cancer. The aim of this study was to investigate the deletion / insertion of 40 bp of mdm2 polymorphism and 16-bp of p53 polymorphism in patients with lymphoma. In this case-control study, 152 non-Hodgkin's lymphoma patients and 155 healthy individuals were selected by convenience sampling method. MDM2 and P53 polymorphisms were examined by PCR. SPSS V22 software was used to interpret the results. The results of the study showed that rs3730485 species was associated with the risk of lymphoma. The Ins / Del and Del / Del genotypes reduce the risk of lymphoma compared to the Ins / Ins genotype (OR = 0.41,95% CI = 0.25-0.65 P <0.001 and OR = 0.33.95% CI = 0.12). 0.96, P = 0.035 In relation to Ins / Del + Del / Del and Ins / Del, the results also showed that they reduce the risk of lymphoma (OR = 0.40,95% CI = 0.25-0.63, P <0.001). Results indicated that Ins / Del, Del / Del, Ins / Del + Del / Del and D alleles genotypes had a statistically significant relationship with lymphoma risk. Due to the fact that the OR genotypes of this site are less than one step away, it is a protective factor for lymphoma, so that these alleles and the mentioned genotype reduce the risk of lymphoma.
Keywords: Humans; Tumor Suppressor Protein p53; Polymorphism, Genetic; Neoplasms; Genotype; Lymphoma
Corresponding author: Kamran Roudini, Department of Internal Medicine, School of Medicine, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran. Email: Kroudini@sina.tums.ac.ir
Published in „GENETIKA“ Vol. 53, No. 3(2021), pp. 1007-1015
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2103007S
Original scientific paper
ASSOCIATION BETWEEN ISOLATED MILD SYMMETRICAL VENTRICULOMEGALY AND FETAL CHROMOSOMAL ABERRATIONS
Jelena STAMENKOVIĆ1, Bojana PETROVIĆ2, Nela MAKSIMOVIĆ1, Jovana JOKSIMOVIĆ3, Luka JOKSIMOVIĆ4, Olivera KONTIĆ-VUČINIĆ1
1Faculty of Medicine, University of Belgrade, Belgrade, Serbia
2Clinic for Gynecology and Obstetrics, Clinical center of Serbia, Belgrade, Serbia
3Medicines and Medical Devices Agency of Serbia, Belgrade, Serbia
4Serbian Institute of Occupational Health “Dr Dragomir Karajovic”, Belgrade, Serbia
Abstract
Stamenković J., B. Petrović, N. Maksimović, J. Joksimović, L. Joksimović, O. Kontić-Vučinić (2021). Association between isolated mild symmetrical ventriculomegaly and fetal chromosomal aberrations. - Genetika, Vol 53, No.3, 1007-1015.
The identification of mild fetal ventriculomegaly prompts further evaluation focused on determining whether additional structural anomalies, genetic abnormalities or congenital infections are present. The aim of this study is to evaluate the frequency of chromosomal aberrations in fetuses with isolated mild symmetrical ventriculomegaly and determine the risk for a fetus with isolated mild ventriculomegaly to have chromosomal abnormality in a back ground. Additionally, we have performed an evaluation of the chromosomal microarray findings in a series of five fetuses with isolated mild symmetrical ventriculomegaly and a normal karyotype. The retrospective observational study included karyotype evaluation of 102 fetuses with isolated mild symmetrical ventriculomegaly identified at the time of the routine midpregnancy scanning. In five cases array-CGH was performed and the obtained data were compared with the data in the bioinformatics databases. Among fetuses with isolated mild symmetrical ventriculomegaly chromosome aberrations were found in 2 (1,96%) fetuses. In both cases autosomal aneuploidy was detected, and those are trisomy 21 and trisomy 18, respectively. The finding of a mild symmetrical isolated ventriculomegaly on the routine ultrasound fetal exam in the second trimester had low sensitivity, but high specificity and negative predictive value in the prediction of chromosome anomalies.
Copy number variants (microduplications/microdeletions) were detected in four cases (80%). A search for the similar variants in NCBI ClinVar, DECIPHER, OMIM and ENSEMBL data bases, revealed that the microdeletions/microduplications detected in four fetuses in our study cannot be related with ventriculomegaly development. Our findings suggest that karyotyping is not justified in fetuses with isolated mild symmetrical ventriculomegaly (10–15 mm), in a low risk population. Therefore, when mild fetal ventriculomegaly is found in a low risk population, additional non-invasive procedures for chromosome aberration screening (such as noninvasive prenatal screening based on cell-free fetal DNA) are recommended, before making the decision to perform invasive diagnostic procedures.
Key words: fetal ventriculomegaly, chromosome aberration, microdeletions /microduplications
Corresponding author: Bojana Petrović, Clinic for Gynecology and Obstetrics, Clinical center of Serbia, Belgrade, Serbia, e-mail: mrdrbojaninmail@gmail.com
Published in „GENETIKA“ Vol. 53, No3(2021), pp1017-1029
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2103017G
Original
scientific paper
NEUROLOGICAL MANIFESTATIONS IN PAKISTANI LYSOSOMAL STORAGE DISORDERS PATIENTS AND MOLECULAR CHARACTERIZATION OF GAUCHER DISEASE
Rutaba GUL1, Sabika FIRASAT1*, Mulazim HUSSAIN2, Muhammad TUFAIL1,
Waheed AHMAD1, Kiran AFSHAN1
1Department of Zoology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan
2 The Children Hospital, Pakistan Institute of Medical Sciences, Islamabad, Pakistan
Abstract
Gul R., S. Firasat, M. Hussain, M. Tufail, W. Ahmad, K. Afshan (2021). Neurological manifestations in Pakistani lysosomal storage disorders patients and molecular characterization of Gaucher disease. - Genetika, Vol 53, No.3, 1017-1029.
Lysosomal storage disorders (LSDs) are a large group of inborn errors of metabolism each caused by genetic mutations of a particular lysosomal protein encoding gene. These inherited conditions are characterized by lysosomal dysfunction with wide variety of organ impact sometimes organ failure with growing age. Neurological complications in LSD cases range from severe neurodegenerations in 70% cases to mild symptoms or absence of neuropathy in others. Each LSD is monogenic but heterogeneous from a molecular standpoint with a large number of mutations described in the respective gene. Some mutations are particular to specific populations, reflecting consequences of founder effect. Present study aimed to access the demographic and clinical profiles of forty-five LSD affected families enrolled during January 2018 to December 2019 at local hospitals to find out neurological symptoms in Pakistani LSD cases. Furthermore, molecular genetic analysis of Gaucher’s disease affected families was performed to unveil underlying disease causing mutation/s. Neurological manifestations were present in twenty-eight families including eleven Mucopolysaccharidosis-1 (MPS-I), four Gaucher’s disease (GD) and all MPS-II, MPS-III, Niemann-Pick, Griscelli and Chediak-Higashi cases. Neurological involvement was not found in eight MPS-I, one GD, all MPS-IV and Pycnodysostosis affected families. Screening of GBA gene in GD families revealed a reported missense mutation p.L483P in all analyzed families. Clinical heterogeneity of MPS-1 and GD is evident from literature however mutational analysis of all enrolled GD families depicted segregation of a reported missense variant p.L483P of GBA gene with disease phenotype in all families. Our findings highlight importance of homeostatic role of lysosomes in neuronal development as twenty eight out of forty families had neurological manifestations. Furthermore, identification of same mutation in GD patients with or without neuronal involvement may be related to some unknown differences in the expression of genetic modifiers or exposure to environmental triggers.
Key words: Lysosomal Storage Disorders, Gaucher Disease, Neurological manifestation, GBA gene, Pakistan
Corresponding author: Sabika Firasat, Department of Zoology, Faculty of Biological Sciences, Quaid-i-Azam University, University road, 45320, Islamabad Pakistan, Telephone: 00925190643209, E-mail: sabika.firasat@qau.edu.pk.
Published in „GENETIKA“ Vol. 53, No.3 (2021), pp.1031-1042
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
633
https://doi.org/10.2298/GENSR2103031K
Original scientific paper
Correlation dependences on quantitive signs in grass pea
(Lathyrus sativus L.) accessions
Valentin KOSEV and Viliana VASILEVA
Institute of Forage Crops, Pleven, Bulgaria
Abstract
Kosev V. and V. Vasileva (2021). Correlation dependences on quantitive signs in grass pea (Lathyrus sativus L.) accessions. - Genetika, Vol 53, No.3, 1031 - 1042.
To assess the phenotypic and genotypic relationships among basic quantitative indicators in grass pea the field experiment was performed in the Institute of Forage Crops, Pleven, Bulgaria during the period of 2014-2016. Correlation (r) and regression (R) analysis were applied. Six grass pea accessions were used. For the most of the indicators, phenotypic relationships were found slightly lower than the values of the coefficients of their genetic correlations. The next phenotypic and genotypic correlation coefficients were distinguished with significant medium to strong relationships: fresh plant weight with: number of leaves (r=0.99; r=0.86; r=0.88), fresh leaves weight (r=0.98, r=0.96, r=0.99) and fresh stems weight (r=0.85; r=0.97; r=0.91); fresh leaves weight with: number of branches (r=0.66; r=0.69; r=0.58) and number of pods (r=0.48; r=0.12; r=0.48); number of branches with: fresh plant weight (r=0.52, r=0.69; r=0.62), number of leaves (r=0.54, r=0.53; r=0.49) and fresh leaves weight (r=0.66, r=0.69; r=0.58). Most significant effect for the formation of seed productivity under the different conditions of the years of study have the signs of fresh root weight (R=4.829; R=2.449; R=0.01), dry stems weight (R=0.369; R=0.507; R=0.387), fresh leaves weight (R=0.297; R=0.101; R=0.097), fresh stems weight (R=0.059; R=2.027; R=0.95) and number of pods (R=0.184; R=0.095; R=0.052), but negatively exert a fresh plant weight (R= - 0.044; R= - 1.882; R= - 1.017) and nodule number (R= - 0.04; R= - 0.03; R= - 0.034). In the selection work the seed productivity can be increased by paying attention to these signs.
Key words: grass pea, nodules, productivity, regression, root mass
Corresponding author: Viliana Vasileva, Institute of Forage Crops, Pleven, Bulgaria, E-mail: viliana.vasileva@gmail.com, phone: + 359 886 06 54 61
Published in „GENETIKA“ Vol. 53, No. 3(2021), pp. 1043-1064
© 2021 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2103043M
Original scientific paper
MORPHOLOGICAL CHARACTERIZATION OF SPONGE GOURD (Luffa aegyptiaca Mill.) GENOTYPES FROM THE EASTERN MEDITERRANEAN REGION OF TURKEY
Kazım MAVİ1, Kazim GÜNDÜZ2, Fulya UZUNOĞLU1, Fırat Ege KARAAT3*
1Hatay Mustafa Kemal University, Faculty of Agriculture, Department of Horticulture, Hatay/Turkey
2Malatya Turgut Özal University, Faculty of Agriculture, Department of Horticulture, Malatya/Turkey
3Adıyaman University, Faculty of Agriculture, Department of Horticulture, Adıyaman/Turkey
Abstract
Mavi K., K. Gündüz, F. Uzunoğlu, F. E. Karaat (2021). Morphological characterization of sponge gourd (Luffa aegyptiaca mill.) genotypes from the eastern Mediterranean region of Turkey- Genetika, Vol 53, No.3, 1043 - 1064.
Having wide range of genetic resources, Hatay is the largest sponge gourd producer of province of Turkey. This study was conducted to determine the status and morphological diversity of the livestock breeding with a field survey in the districts of Hatay Province. As a result of the survey, 34 genotypes were collected that thought to be morphologically different. When the distributions of genotypes were examined, the genotypes were selected from Arsuz (5 genotypes), Defne (7 genotypes), Samandağ (1 genotypes), Antakya (5 genotypes), Dörtyol (6 genotypes), Yayladağı (4 genotypes), Altınözü (4 genotypes), Payas (1 genotypes) and Erzin (1 genotypes). Two genotypes (‘31 AN 03’ and ‘31 AL 04’) from which the seeds did not germinate were extracted from the morphological characterization studies. Thirty-two genotypes selected from Hatay with 3 genotypes obtained from Adana, South Korea, and China in total of 35 genotypes were compared in terms of 49 different morphological characteristics. Variations between the genotypes were not identified for the observations made on these 49 features regarding cotyledon color, stem shape, tendrils, leaf margin, growth habit, sex type and peduncle separation from fruit. As a result of analysis of basic components and cluster analysis based on morphological characterization features, genotypes were included in six different groups. However, the effect of the districts on which genotypes were collected on this grouping could not be determined.
Keywords Cucurbits, Genetic diversity, Morphologic characterization, Sponge gourd
Corresponding author: Fırat Ege Karaat, Adıyaman University, Faculty of Agriculture, Department of Horticulture, Adıyaman/Turkey, E-mail: fkaraat@adiyaman.edu.tr
Published in „GENETIKA“ Vol. 53, No.3 (2021), pp. 1065-1079
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2103065A
Original
scientific paper
MOLECULAR BASED IDENTIFICATION OF Tem ß-lactamase AND Tet A RESISTANCE GENE IN E. coli
Madiha AMJAD1, Qaisar AKRAM1,2, Humara KAUSER1, Azam ALI1*, Qurban ALI1*
1Institute of Molecular Biology and Biotechnology, the University of Lahore, Lahore
Pakistan
2Department of Microbiology, University of veterinary and Animal Sciences, Lahore, Pakistan
Abstract
Amjad M., Q. Akram, H. Kauser, A. Ali, Q. Ali (2021). Molecular based identification of Tem ß-lactamase and Tet a resistance gene in E. coli. - Genetika, Vol 53, No.3, 1065 - 1079.
Escherichia coli is a universal bacterium causing infections in humans and animal and serves as a major pathogen of urinary tract infections (UTI) and Extraintestinal infection. The present study was conducted for current antibiotic resistance pattern of E. coli and molecular detection of resistance related gene in clinical isolates of E. coli. The study was a hospital based, prospective study which was done for a period of twelve months. This study was done by using the standard culture techniques for urine, pus, semen and sputum samples, Maximum number was from samples of urine 73 followed by pus 23 semen 2 and sputum 2. Hundred pathogenic E. coli isolates was further identified by standard microbiology techniques such as colony morphology, Gram staining and biochemical testing methods. Drug resistance was evaluated by disc diffusion method and relevant drug resistance gene detection done by Multiplex PCR. Out of 130 clinical samples total (n=100) isolates were identified as E. coli and their susceptibility patterns for different antibiotics were determined. Results showed that Gentamicin among aminoglycosides and Colistin sulfate among polymyxin were showed relatively less resistance in E. coli. Bacitracin, Ampicillin, trimethoprim, Erythromycin, Tetracycline, Ciprofloxacin, Amoxicillin and Piperacillin were found more resistant. Imepenum, Meropenum among β lactam were most effective drug. PCR was employed to identify resistance causing gene. Among 100 pathogenic E. coli isolates 87% shown ampicillin resistance encoded by Tem B lactamase gene and 86 % shown tetracycline resistance causing by Tet A gene. Highest level of drug-resistance was observed against ampicillin and tetracycline (AMP-TET) among clinical isolates of pathogenic E. coli collected from hospitalized patients.
Key words: Escherichia coli, antibiotics, pathogen, drugs, molecular detection
Corresponding author: Azam Ali, Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan. E-mails: azam.skyblue@gmail.com, saim1692@gmail.com
Published in „GENETIKA“ Vol. 53, No. 3(2021), pp. 1081-1092
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2103081J
Original
scientific paper
RAPD PROFILING IN DETECTING GENETIC VARIATION IN Glaucium (Papaveraceae) SPECIES: EDIBLE AND MEDICINAL PLANT
Lei JIAO¹,*, HuaPing XIAO²#, Xu ZHAO3#, Faezeh Mirazimi ABARGHUEI4
1Yale School of Medicine, Yale University, New Haven, CT,06510 , USA
2.Department of Anesthesiology, Jiangxi Cancer Hospital, Nanchang, Jiang Xi,330029, China
3.Department of Anesthesiology,The Second Xiangya Hospital, Central South University, Changsha,Hu Nan,410011,China
4Department of Horticultural Sciences, School of Agriculture, Shiraz University, Shiraz 71441-65186, Iran
Abstract
Jiao L., H. Xiao, X. Zhao, F. M. Abarghuei (2021). RAPD profiling in detecting genetic variation in Glaucium (Papaveraceae) species: Edible and Medicinal plant. - Genetika, Vol 53, No.3,1081 - 1092.
Glaucium is a genus belonging to Papaveraceae subfam. Chelidonoideae Ernest that contains about 23 species. The distribution of Glaucium species relatively widely covers western Asia and the Mediterranean region and is decreased from central Asia to the European countries. As a country, Iran harbors relatively more species of the genus Glaucium (10 species) and hence, this country is considered as the hot spot of the genus. No detailed Random Amplified Polymorphic DNA (RAPD) studies were conducted to study Glaucium genetic diversity. Therefore, we collected and analyzed three species from 6 provinces of Iran regions. Overall, 60 plant specimens were collected. Our aims were 1) to assess genetic diversity among Glaucium species 2) is there a correlation between species genetic and geographical distance? 3) Genetic structure of populations and taxa. We showed significant differences in quantitative morphological characters in plant species. G. flavum var. serpieri depicted unbiased expected heterozygosity (UHe) in the range of 0.188. The Mantel test showed correlation (r = 0.66, p=0.0001) between genetic and geographical distances. We reported high genetic diversity, which clearly shows the Glaucium species can adapt to changing environments since high genetic diversity is linked to species adaptability. Present results highlighted the utility of RAPD markers and morphometry methods to investigate genetic diversity in Glaucium species.
Key words: Gene Flow, Random Amplified Polymorphic DNA (RAPD), Glaucium Species, Isolation, Morphometry.
Corresponding author: Lei Jiao, Yale School of Medicine, Yale University, New Haven, CT,06510, USA, luzyueyy@163.com; #HuaPing Xiao are Co-First author, Xu Zhao are Second author
Published in „GENETIKA“ Vol. 53, No3 (2021), pp1093-1103
© 2021 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575. 633.11
https://doi.org/10.2298/GENSR2103093T
Original
scientific paper
SALT STRESS TOLERANCE POTENTIAL OF SELECTED RICE CULTIVARS
OF PAKISTAN
Faiza TAWAB* 1, Afaq AHMAD3, Adnan NASIM2, Saleha TAWAB2, Iqbal MUNIR3
1 Shaheed Benazir Bhutto Women University, Larama Peshawar, Pakistan
2Agriculture Research Khyber Pakhtunkhwa, Pakistan
3 Institute of Biotechnology & Genetic Engineering, The University of Agriculture, Peshawar, Pakistan
Abstract
Tawab F., A. Ahmad, A. Nasim, S. Tawab, I. Munir (2021). Salt stress tolerance potential of selected rice cultivars of Pakistan. - Genetika, vol 53, no.3, 1093-1103.
Ten local rice varieties (Sarshar, Pakhal, Sada Hayat, Jajai-77, NIR-9, RI-DR-92, JP-5, Shaheen Basmati, Shadab-31 and TN-1) were evaluated for salinity stress (0, 50, 75 and 100 mM of NaCl) tolerance at seedling stage, in Completely Randomized Design (CRD). On an average the root length, shoot length and the relative growth rate of all rice varieties decreased linearly with increased levels of salinity. Based on the individual results of all the parameters studied, Pakhal was found to be the most salt tolerant variety. The germination rate of the varieties RI-DR-92 and TN-1 was better at higher levels of salinity (75 and 100 mM NaCl) among all the tested varieties. While, considerable reduction in germination rate was recorded for Jajai-77. The varieties RI-DR-92 and TN-1 that were more affected showing more reduction in shoot length (4.2 cm and 4.4 cm), root length (2.9 and 3.2 cm) and relative growth rate (0.22 and 0.14 g/gday), grown at 75 and 100 mM NaCl respectively. The variety Pakhal showed less reduction in shoot length (1.5 and 2.7 cm), root length (0.95 and 1.0 cm) and relative growth rate (0.073 and 0.075 g/gday), grown at 75 and 100 mM NaCl, respectively as compared to control. However, according to Stress Susceptibility Index (SSI), TN-1 was observed to be the most tolerant and Jajai-77 was the most susceptible variety to salt stress. The most tolerant rice varieties differentiated in the current study could be used to produce higher yields under saline conditions.
Keywords: Salinity, Stress Tolerance, Rice, Cultivars, Seedling stage
Corresponding author: Faiza Tawab, Institute of Biotechnology and Genetic Engineering, the University of Agriculture Peshawar, Pakistan, E-mail: faizatawab@gmail.com, Phone:+923369966726
Published in „GENETIKA“ Vol. 53, No. 3(2021), pp. 1105-1115
© 2021Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2103105B
Original scientific paper
MATHEMATICAL MODELING FOR GENOMIC SELECTION IN SERBIAN DAIRY CATTLE
Radmila BESKOROVAJNI1, Rade JOVANOVIĆ1, Lato PEZO2, Nikola POPOVIĆ1, Nataša TOLIMIR1, Ljubiša MIHAJLOVIĆ3, Gordana ŠURLAN-MOMIROVIĆ4
1 Institute for Science Application in Agriculture, Belgrade, Serbia
2 Institute for General and Physical Chemistry, University of Belgrade, Belgrade, Serbia
3 Ministry of Agriculture, Forestry and Water Management, Belgrade, Serbia
4 University of Belgrade, Faculty of Agriculture, Belgrade, Serbia
Abstract
Beskorovajni R., R. Jovanović, L.Pezo, N. Popović, N.Tolimir, Lj. Mihajlović, G. Šurlan-Momirović (2021). Mathematical modeling for genomic selection in serbian dairy cattle. - Genetika, Vol 53, No.3, 1105-1115.
This manuscript has come as a result of an efficient breeding program in Serbian cattle populations for some economically important traits. Genomic selection in the last two decades has been the main challenge in animal breeding programs and genetics. Many SNP markers are used in statistical analysis in predicting the accuracy of breeding values for young animals without their performance. The new breeding tendency in the selection of young animals allows their genetic progress with reducing cost. In this study, 92 Holstein cows from various regions in Serbia were analyzed based on SNP molecular markers. Within this investigation, an empirical model was developed for the prediction of Yield Traits and Fertility Traits variables, according to Key traits data for dairy cattle. The developed model gave a reasonable fit to the data and successfully predicted Yield Traits (such as Fat and Protein Percent, Cheese Merit, Fluid Merit, and Cow Livability) and Fertility Traits variables (such as Sire Calving Ease, Heifer Conception Rate, Cow Conception Rate, Daughter Stillbirth, Sire Stillbirth, and Gestation Length). A total of 92 dairy cattle data were used to build a prediction model for the prediction of Yield Traits and Fertility Traits variables. The artificial neural network model, based on the Broyden-Fletcher-Goldfarb-Shanno iterative algorithm, showed good prediction capabilities (the r2 values during the training cycle for the before mentioned output variables were in the range between 0.444 and 0.989).
Keywords: Mathematical modeling, genetic evaluation, single-nucleotide polymorphism, SNP, haplotype, genotyping.
Corresponding author: Gordana Šurlan-Momirović, Faculty of Agriculture, University of Belgrade, Belgrade, Serbia, Tel: 064 31 70 511. E-mail: surlang@gmail.com
Published in „GENETIKA“ Vol. 53, No.3(2021), pp. 1117-1131
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633
https://doi.org/10.2298/GENSR2103117T
Original scientific paper
SUNFLOWER GENOTYPES TOLERANCE TO CHARCOAL ROT
(Macrophomina phaseolina (TASSI) GOID.) UNDER THE FIELD CONDITIONS
Sonja TANČIĆ ŽIVANOV*, Boško DEDIĆ, Sandra CVEJIĆ, Siniša JOCIĆ,
Vladimir MIKLIČ
Institute of Field and Vegetable Crops, Novi Sad
Abstract
Tančić Živanov S., B. Dedić, S. Cvejić, S. Jocić, V. Miklič (2021). Sunflower genotypes tolerance to charcoal rot (Macrophomina phaseolina (tassi) goid.) under the field conditions. - Genetika, Vol 53, No.3, 1117 - 1131.
Sunflower (Helianthus annuus L.) is one of the most important crops grown in the world, but it can be attacked by more than 30 different pathogens. The areal of Macrophomina phaseolina (Tassi) Goid., one of the sunflower pathogens nowadays often found in temperate regions, has been spreading over the last few years due to climate change. The most effective eco-friendly method for controlling charcoal rot caused by M. phaseolina is growing resistant sunflower cultivars. Due to that, 24 commercially available hybrids and 70 inbred lines were tested for M. phaseolina tolerance in a two-year trial. Under the field conditions, two different inoculation methods were used – the Unwounded Stem Base Inoculation (USBI) and Toothpick (TP) method. This study identified five highly tolerant hybrids and 12 inbred lines that can be used in breeding programs for improvement of future genotypes. Sunflower genotype screening tests for Macrophomina tolerance indicated that both inoculation methods should be applied together to provide reliable results, and that stem lesion length is a reliable trait for disease severity assessments.
Key words: charcoal rot; inoculation methods; Helianthus annuus, Macrophomina phaseolina
Corresponding author: Sonja Tančić Živanov, Institute of Field and Vegetable Crops, Maksima Gorkog 20, 21000 Novi Sad. E-mail: sonja.tancic@ifvcns.ns.ac.rs; phone: +381 64 8706073
Published in „GENETIKA“ Vol. 53, No. 3(2021), pp. 1133-1146
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2103133A
Original scientific paper
ASSESSMENT OF FRUIT FLESH BROWNING IN ‘AMASYA’ APPLE CULTIVAR AND ITS TWO SELECTED SIBS
Ayşe Nilgün ATAY*
Abstract
Atay A. N. (2021). Assessment of fruit flesh browning in ‘Amasya’ apple cultivar and its two selected sibs.)- Genetika, Vol 53, No.3, 1133-1146.
Post-cut internal flesh browning of apples is a major limitation to consumer acceptability as it causes to the unattractive appearance and consequential off-flavour for fresh fruit. The browning of apples is also essential for processed products such as fresh-cut, fruit juice, canned, frozen, and drying. In this work, internal flesh browning susceptibility was investigated in ‘Amasya’ apple cultivar and two superior sibs (named ‘109’ and ‘120’), of which female parent is ‘Amasya’, for both visually and objectively. Colour parameters of the cutting surface were measured every ten minutes during the first thirty minutes after slicing. The most rapid initial browning of fruit flesh occurred within the first ten minutes after the disruption in both sibs and ‘Amasya’. It was observed that ‘Amasya’ undergone significant colour change first, followed by ‘120’ in all the studied colour parameters, whereas ‘109’ did not brown significantly. Hence, it can be concluded that ‘109’ could be useful in the fruit processing industry thanks to the remarkable post-cut anti-browning properties. Valuable correlations were found between pH and the colour parameters of cutting surface in the thirty minutes after slicing. However, the colour change parameters were not or weakly correlated to other fruit attributes. The results of this study show the importance of the internal flesh quality of fruit in breeding programs to maintain quality.
Keywords: Enzymatic browning, polyphenol oxidase, breeding, fruit quality, processing
Corresponding author: Ayşe Nilgün Atay, Burdur Mehmet Akif Ersoy University, Food Agriculture and Livestock School, Department of Crop and Livestock Production, Horticulture Program, Burdur, Turkey. E-mail: anatay@mehmetakif.edu.tr
Published in „GENETIKA“ Vol. 53, No. 3(2021), pp. 1147-1165
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575. 633.15
https://doi.org/10.2298/GENSR2103147B
Original
scientific paper
PHYSIOLOGICAL AND GENE EXPRESSION CHANGES DURING IMBIBITION
IN MAIZE SEEDS UNDER LOW TEMPERATURE CONDITIONS
Manja BOŽIĆ*1, Aleksandra STANOJEVIĆ2, Ksenija MARKOVIĆ1, Dragana IGNJATOVIĆ MICIĆ1, Dragana NIKOLIĆ3, Marija MILIVOJEVIĆ1, Ana NIKOLIĆ1
1Maize Research Institute „Zemun Polje“, Belgrade, Serbia
2 Faculty of Biology, University of Belgrade, Belgrade, Serbia
3Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia
Abstract
Božić M., A. Stanojević, K.Marković, D. Ignjatović Micić, D.Nikolić, M. Milivojević, A. Nikolić (2021). Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions. - Genetika, Vol 53, No.3, 1147-1165.
Maize is one of the most important crop species worldwide, but also extremely susceptible to the effects of increasingly higher temperatures and drought during the summer and its flowering and grain filling stage. Different strategies are being utilized to ensure a satisfying yield potential and quality even in the extremely unfavourable environmental conditions, which are the result of climate change. Some of them are cropping pattern changes and sowing alterations, including earlier sowing. Since this implies the exposure to suboptimal temperatures during early developmental stages, it leads to a demand for developing maize lines tolerant to low temperatures during these stages. This research focuses on the first phase of germination, imbibition.
Maize tolerance to low temperatures is a complex trait that includes different mechanisms and strategies that all work together to ensure adaptation and survival, such as cell membrane changes, antioxidative system activation, etc. Additionally, the low temperature response of different maize inbreds varies substantially, so recognizing the different ways in which they respond to low temperatures during imbibition and other earlier stages of development is crucial. To accomplish this, seeds of two maize inbred lines of contrasting susceptibility to low temperatures were selected and exposed to control (20° C) and low temperature (8°C) conditions during the first 24h of imbition and then further analyzed to assess their response. This included germination and tetrazolium assays; ascertaining membrane integrity by evaluating cell leakage and lipid peroxidation; determining the antioxidative capacity by assessing superoxide dismutase (SOD) and catalase (CAT) activity; and expression analysis of four genes included in the low temperature response (gibberellin insensitive dwarf 1 gibberellin receptor, gid1; fatty acid desaturases 2 and 6, fad2 and fad6; plastid-lipid-associated 2 protein, pap2).
The results showed that, while there is not a significant difference in their germination rate, they differ in their survival rate, with more seeds of the tolerant genotype surviving the low temperature period. Significant differences between them were found in cell leakage (p<0,01), as well as gid1(p<0,05) and fad6 (p<0,05) gene expression assays. The present research brings light to our understanding of the effect of low temperatures on the first germination stage, - imbibition. It highlights the importance of choosing the right inbreds for earlier sowing and points to certain routes that could be taken for improving and accelerating the breeding process for low temperature tolerance.
Key words: maize, imbibition, low temperature tolerance, abiotic stress response
Corresponding author: Manja Božić, Maize Research Institute „Zemun Polje“, Slobodana Bajića 1, 11185, Belgrade, Serbia, Email: mbozic@mrizp.rs; Tel.: +381648406221
Published in „GENETIKA“ Vol. 53, No. 3(2021), pp.1167-1178
© 2021 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2103167Z
Original scientific paper
ASSOCIATION OF RS1294845152 IN CRISP2 WITH MALE INFERTILITY:
RFLP AND DNA SEQUENCE ANALYSIS
Fahimeh ZANGANEHa and Masoud SHEIDAIb*
aDepartment of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran
bDepartment of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
Abstract
Zanganeh F. and M. Sheidai (2021). Association of rs1294845152 in CRISP2 with male infertility: RFLP and DNA sequence analysis. - Genetika, Vol 53, No.3, 1167-1178.
Infertility is a major health human problem with 10% to 15% of world-wide occurrence. It may happen due to various reasons including physiological, environmental, social, and genetic factors. Recent genetic investigations show that hundreds of genes may act on infertility, each with minor effects. Asthenozoospermia is one of the common types of infertility in men which is determined by sperm motility reduction. CRISP2 gene is located on acrosome and tail of sperm and plays crucial role in spermatogenesis, regulation of sperm flagellum motility, acrosome reaction and gamete fusion. In general, heterogeneous results are present for association of CRISP2 gene with asthenoteratozoospermic male infertility, therefore, we carried out a starting investigation to show association of rs1294845152 of CRISP2 Gene with male infertility by RFLP analysis. We also tried to identify the known SNPs and variant nucleotides adjacent to this SNP by sequencing. The present study revealed that neighther rs1294845152, nor the neighboring sequences show any association with this kind of male infertility in the limited samples studied in Iran. In general, it seems that this part of men genetic material shows a high degree of conservation and very low level of nucleotide variability.
Key words: Association study, clustering, Nucleotide diversity, RFLP, TCS-Network.
Corresponding author: Masoud Sheidai, Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran. E-mail: msheidai@yahoo.com
Published in „GENETIKA“ Vol. 53, No. 3(2021), pp1179-1192
© 2021 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2103179B
Original
scientific paper
MOLECULAR SYSTEMATIC STUDIES IN THE GENUS Glaucium (Papaveraceae)
Zahra BOUZARISARAVANI1, Fariba SHARIFNIA1*, Fahimeh SALIMPOUR1,
Sedigheh ARBABIAN1, Afsaneh GERAN2
1Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran
2Department of Biology, Gorgan Branch, Islamic Azad University, Gorgan, Iran
Abstract
Bouzarisaravani Z., F. Sharifnia, F. Salimpour, S. Arbabian, A. Geran (2021). Molecular systematic studies in the genus Glaucium (Papaveraceae). - Genetika, Vol 53, No.3, 1179-1192.
Glaucium is mainly distributed from Atlantic Europe to Central Asia. The genus comprises two sections and 23 species: sect. Acropetala Mory with four species, four subspecies and two varieties and sect. Glaucium with 19 species, eight subspecies and 16 varieties. Species identification is fundamentally important within the fields of biology, biogeography, ecology and conservation. There are 10 species including 4 subspecies and 14 varieties of Glaucium in Iran. Taxonomy and phylogeny of the genus is highly complicated and controversial. The present study was done by use of phenetic analyses of morphological characters as well as Bayesian analyses of molecular data (ITS sequences) to illustrate the species relationships, taxonomic classification, monophyly versus paraphyly of the species in the genus Glaucium. We used ten Glaucium species for molecular studies, of which, nrDNA, ITS sequences were newly obtained for 7 species. The molecular analysis, based on successive reweighting by rescaled consistency index, revealed that Maximum parsimony, maximum likelihood and Bayesian methods gave very similar results based on ITS dataset. In general, the present study revealed that the species could be differentiated by morphological characters. PCA and cluster analysis (Ward’s method) carried out for morphological traits divided the Glaucium species in to two cluster. Phylogenetic relationships within Glaucium are known and ITS-based phylogenetic trees and morphological characters were in concordance.
Key words: Cladistics, Glaucium, ITS, Phenetic, Phylogeny
Corresponding author: Fariba Sharifnia, Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran. E-mail: fa.sharifnia@gmail.com, f_sharifnia@iau_tnb.ac.ir
Published in „GENETIKA“ Vol. 53, No. 2(2021), pp. 1193-1205
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575. 630
https://doi.org/10.2298/GENSR2103193O
Original
scientific paper
MORPHOMETRIC ANALYSIS AND GENETIC DIVERSITY IN Pistacia SPECIES POPULATIONS USING SEQUENCE RELATED AMPLIFIED POLYMORPHISM
Chun OU1,2, Zhongyuan SHEN1, Yu LIU3,*, Zelu WANG1 and Mohsen FARSHADFAR4
1School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, Anhui, China
2Engineering Technology Research Center of Anti-aging Chinese Herbal Medicine, Fuyang 236037, Anhui, China
3School of Architecture and Engineering, Suqian College, Suqian 223800, Jiangsu, China
4Department of Agriculture, Payame Noor University (PNU), Tehran, Iran
Abstract
Ou C., Z. Shen, Y. Liu, Z. Wang, M. Farshadfar (2021). Morphometric analysis and genetic diversity in Pistacia species populations using sequence related amplified polymorphism. - Genetika, Vol 53, No.3, 1193-1205.
The Anacardiaceae family includes 11 or more species, including the genus Pistacia. Pistacia vera L. is the most commercially developed of them all, thanks to its tasty nut seeds. Pistacia khinjuk Stocks, Pistacia atlantica Dsef, Pistacia vera, Pistacia eurycarpa Yalt. (Pistacia atlantica subsp. Kurdica Zoh.) and all have their origins in Iran. The present study aimed to investigate the SRAP (Sequence-related amplified polymorphism) markers in 13 wild pistachio accessions, which comprised three different species: Pistacia khinjuk, Pistacia vera, and Pistacia atlantica. Through polymerase chain reaction amplifications (PCR) of three Pistacia species, a total of 170 (Number of total loci) (NTL) DNA bands were obtained. Ten different selective primers were combined to generate these bands. The number of amplified pieces ranged from nine to twenty-six. The projected impartial gene diversity (UHe) ranged from 0.053 (Pistacia khinjuk) to 0.417 (Pistacia khinjuk) (Pistacia atlantica subsp. Kurdica). The genetic similarity of three species is estimated to be between 0.61 to 0.90. Two significant groupings emerged from the clustering findings: Pistacia khinjuk and Pistacia atlantica subsp. Kurdica exhibited the least similarity in the SRAP markers study. Our findings revealed excellent molecular recognition of all genotypes tested, indicating that a significant amount of genetic variety exists among pistachio accessions. This discovery might be useful in breeding management techniques for genetic preservation and cultivar improvement.
Key words: Gene Flow, Genetic Diversity, Pistacia, Sequence-related amplified polymorphism
Corresponding author: Yu Liu, School of Architecture and Engineering, Suqian College, Suqian 223800, Jiangsu, China, E-mail: 718111060@qq.com; aras0990m@gmail.com
Published in „GENETIKA“ Vol. 53, No. 3(2021), pp. 1207-1217
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2103207O
Original
scientific paper
RUNX3 PROMOTER METHYLATION IS ASSOCIATED WITH ORAL SQUAMOUS CELL CARCINOMA LOCATION
Kenneth C. ONYEGBULA1, Benjamin O. EMIKPE2, Akinyele O. ADISA3,
Chiaka I. ANUMUDU4,*
1Department of Biomedical Laboratory Science, Faculty of Basic Medical Sciences, College of
Medicine, University of Ibadan, Nigeria
2Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Ibadan,
Nigeria
3Department of Oral Pathology, Faculty of Dentistry, College of Medicine, University of
Ibadan, Nigeria
4Department of Zoology, Faculty of Science, University of Ibadan, Nigeria
Abstract
Onyegbula C.K., B. O. Emikpe, A. O. Adisa, C. I. Anumudu (2021). RUNX3 promoter methylation is associated with oral squamous cell carcinoma location. - Genetika, Vol 53, No.3, 1207-1217.
The aetiology of OSCC remains unclear, however, aberrant methylation of CpG island promoters of tumor suppressor genes have been identified as contributory developmental pathways in several cancers. The aim of this study was to determine the presence of RUNX3 gene methylation and how its association with patients’ demographic variables such as gender, age, histologic class and tumor location could be of diagnostic value for OSCC. Sixty-seven formalin-fixed paraffin-embedded (FFPE) solid tissue blocks of OSCC, and nine blocks of benign oral lesions of epithelial origin retrieved from the archives of the Department of Oral Pathology, University College Hospital, Ibadan, South-West Nigeria were used for the analyses. Frequency of CpG island methylation in the promoter region of RUNX3 was determined by methylation-specific polymerase chain reaction (MSP). Association between gender, age, tumor location, histologic class and promoter methylation in RUNX3 was assessed with Pearson’s χ2 test. Overall, 45% (30/67) of OSCC demonstrated methylation in the RUNX3 promoter indicating a high frequency of methylation of the CpG island promoter region of RUNX3. There was no association between gender, age, histologic class and promoter methylation in RUNX3 (P > 0.05), however a significant association was observed between tumor location and promoter methylation of RUNX3 (P < 0.05). Aberrant methylation of the CpG island promoter region of RUNX3 together with tumor location could therefore be critical in the development and diagnosis of OSCC.
Key words: Diagnosis, risk factors, CpG methylation, oral cancers, Ibadan
Corresponding author: Chiaka I. Anumudu, Department of Zoology, Faculty of Science, University of Ibadan, Nigeria, E-mail: cianumudu@yahoo.com; Tel.: 234-802-359-0478.
Published in „GENETIKA“ Vol. 53, No. 3(2021), pp. 1219-1228
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC
575.633.15
https://doi.org/10.2298/GENSR2103219P
Original
scientific article
GENETIC DISTANCE OF MAIZE INBREDS FOR PREDICTION OF HETEROSIS AND COMBINING ABILITY
Sanja PERIĆ 2, Milan STEVANOVIĆ1*, Slaven PRODANOVIĆ2, Snežana MLADENOVIĆ DRINIĆ1, Nikola GRČIĆ1, Vesna KANDIĆ1, Jovan PAVLOV1
1Maize Research Institute Zemun Polje, Belgrade, Serbia
2 Faculty of Agriculture – University of Belgrade, Serbia
Abstract
Perić S., M. Stevanović, S. Prodanović, S. Mladenović Drinić, N. Grčić, V. Kandić, J. Pavlov (2021). Genetic distance of maize inbreds for prediction of heterosis and combining ability. - Genetika, Vol 53, No.3, 1219-1228.
A panel of seven maize inbred lines belonging to Zemun Polje commercial pool were genotypized using SNPs bead chip. 21 hybrids, developed according to the half dialel mating design, were tested in the field together with inbred lines per se. The goal of the study was to determine the genetic distance among seven maize inbred lines and to establish whether there was a significant correlation between the genetic distance among parental inbreds and grain yield, specific combining abilities (SCA) and high-parent (HP) heterosis for the grain yield. The inbred lines ZPL2 and ZPL4 with the genetic distance of 0.487 were the most genetically distant parents, while inbred lines ZPL1 and ZPL2 with the genetic distance of 0.191 were the closest ones. Three subclusters of inbred lines were distinguished in the dendrogram. Inbred lines ZPL5, ZPL6, ZPL3 and ZPL7 were grouped into the first subcluster, while inbred lines ZPL1 and ZPL2, i.e. the inbred line ZPL4 were grouped in the second, i.e. the third subcluster, respectively. The values of the Spearman's rank correlation coefficient between the genetic distance among inbred lines based on the SNP markers, and grain yield, specific combining abilities (SCA) and high-parent (HP) heterosis were positive and statistically significant. The highest correlation coefficient was exhibited between the grain yield and high-parent (HP) heterosis (0.93), and then between the genetic distance and the grain yield (0.92) as well as between the genetic distance and high-parent (HP) heterosis (0.91).
Key words: inbred lines, maize, genetic distance, SNP markers, heterosis
Corresponding author: Milan Stevanović, Maize Research Institute, Slobodana Bajića 1, 11185 Belgrade, email.mstevanovic@mrizp.rs
Published in „GENETIKA“ Vol. 53, No3(2021), pp.1229-1238
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2103229Y
Original
scientific paper
COMPARISON OF INDIVIDUAL BASED APPROACHES USING RAPD MARKERS FOR IDENTIFYING GENETIC RELATIONSHIPS IN Erodium (Geranaiceae)
Xixi YAO1, Rui ZHOU2*, Mohsen FARSHADFAR3
1College of Agriculture and Animal Husbandry, Qinghai University, Xining,Qinghai, 810016, China
2Academy of animal husbandry and Veterinary Sciences, Qinghai University, Xining, Qinghai, 810016, China
3 Department of Agriculture, Payame Noor University (PNU), Tehran, Iran
Abstract
Yao X., R. Zhou, M.Farshadfar (2021). Comparison of individual based approaches using RAPD markers for identifying genetic relationships in Erodium (Geranaiceae)- Genetika, Vol 53, No.3, 1229 - 1238.
Genetic diversity research is required to understand the conservation and management of plant resources in all ecosystems. In Iran, there are 15 different Erodium species. Erodium genetic diversity has not been studied extensively using Random Amplified Polymorphic DNA (RAPD). As a result, six species were gathered and learned from six different Iranian locales. A total of 82 plant specimens were collected. Our objectives were to assess Erodium species genetic diversity and to identify Erodium species genetic diversity. 2) Is there a link between a species's genetic makeup and geographic location? 3) Population and taxon genetic structure. Erodium species were separated into two groups using an unweighted pair group technique with arithmetic mean and principal component analysis. In Erodium, gene flow (Nm) was relatively modest (0.33). The Mantel test revealed a link between genetic and geographical distances (r = 0.77, p=0.0001). We identified many genetic variations in the Erodium species, which indicates that it can adapt to changing circumstances. According to recent findings, RAPD markers and morphometry approaches may investigate Erodium species genetic diversity.
Keywords Gene flow, Random Amplified Polymorphic DNA (RAPD), Erodium species
Corresponding author: Rui Zhou, College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai, 810016, China. E-mail: 13893632442@163.com; jamshidman389@gmail.com;
Published in „GENETIKA“ Vol. 53, No. 3(2021), pp. 1239-1251
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2103239K
Original
scientific paper
RAPID IMMUNODETECTION ASSAY BASED ON SOMATIC AND EXCRETORY SECRETORY ANTIGEN OF Fasciola SPECIES IN LARGE RUMINANTS
Maria KOMAL1, Kiran AFSHAN1*, Seemi ZAFAR1, Muhammad Asim KHAN1, Sabika FIRASAT,1 Mazhar QAYYUM2
1Department of Zoology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan
2Department of Zoology and Biology, Faculty of Sciences, PMAS-Arid Agriculture University, Rawalpindi-46300, Pakistan
Abstract
Komal M., K. Afshan, S. Zafar, M. A. Khan, S. Firasat, M. Qayyum (2021). Rapid immunodetection assay based on somatic and excretory secretory antigen of Fasciola species in large ruminants. - Genetika, Vol 53, No.3, 1239-1251.
Fasciolosis, caused by liver fluke species of the genus Fasciola, are well recognized because of its high veterinary impact. Stool examination for Fasciola eggs is not a sensitive method, and limited efforts to find a reliable and cheaper means of detection are available. The present study aimed to develop rapid diagnostic ELISA test against fasciolosis. The excretory/secretory (ES) and somatic (SA) products of Fasciola helminths were analyzed using polyacrylamide gel electrophoresis (PAGE). Immunogenicity was evaluated by immunoblotting using hyperimmune sera raised in rabbits and seroprevalence was determined by indirect ELISA. The results of SA antigen of Fasciola species showed polypeptide bands ranged from 10kDa-100kDa, while ES antigen of Fasciola showed bands of 15kDa-55kDa. The immunoblotting results showed the most prominent bands against ES antibodies were 25, 35, 55-70, 100 and 250 kDa and SA antigens showed 10, 15-25, 35, 70, 100 and 250 kDa polypeptide bands. The sensitivity and specificity of developed indirect ELISA for SA antigens was 95.45% and 87.1%, while for ES antigens was 100% and 77.42% respectively. The overall seroprevalence recorded for fascioliasis based on SA antigen was 39.8% and 29.8% for ES antigen. The fasciolosis did not show significant association with host type, sex, and age groups of examined animals, however significantly higher infection was found in months of September and October. The result provides sensitive in house immunodetection assay for diagnosis of fasciolosis alternative to commercial kits with high import cost.
Key words: Fasciolosis; Indirect ELISA; Somatic and excretory secretory antigen; Immunoblotting; Pakistan
Corresponding author: Kiran Afshan, Assistant Professor, Department of Zoology, Faculty of Biological Sciences, Quaid-i-Azam University Islamabad, 45320, Pakistan, E-mail: kafshan@qau.edu.pk; Phone:+92 51 90643252
Published in „GENETIKA“ Vol. 53, No. 3(2021), pp 1253-1272
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633
https://doi.org/10.2298/GENSR2103253U
Original
scientific paper
Investigation on Morphology and Physiology of Nitrogen Efficiency in Different Pepper (Capsicum annuum L.) Inbred Lines
Firdes ULAS1*, Saliha ERDOGDU1, Halit YETISIR1, Abdullah ULAS2
1Department of Horticulture, Faculty of Agriculture, Erciyes University, Kayseri, Turkey
2Department of Soil Science and Plant Nutrition, Faculty of Agriculture, Erciyes University, Kayseri, Turkey
Abstract
Ulas F., S. Erdogdu, H. Yetisir, A. Ulas (2021). Investigation on morphology and physiology of nitrogen efficiency in different pepper (Capsicum annuum L.) inbred lines. - Genetika, Vol 53, No.3,1253 - 1272.
In this study, two hydroponic experiments were carried out in a nutrient solution growth system in a growth chamber to determine the morphological and physiological background of nitrogen efficiency in pepper (Capsicum annuum L.). In the first experiment, 16 pepper inbred lines and 2 pepper commercial rootstocks were screened under 2 nitrogen (N) doses (0.3 mM and 3.0 mM N) in a completely randomized block design with 3 replications. In the second experiment, four pepper lines (N-efficient: 21-H-1-1 and AH-2-3, N-inefficient: ERU 1248 and 24-H-6) selected in the first stage of the study were grafted reciprocally and tested under 2 N doses. By using nitrogen efficient lines (21-H-1-1/24-H-6, 21-H-1-1/ERU 1248, AH-2-3/ERU 1248, AH-2-3/24-H-6) as rootstocks increased growth and biomass production compared to non-grafted control plants (N-inefficient), while using N-efficient lines (1248/21-H-1-1, ERU 1248/AH-2-3, 24-H-6/21-H-1-1, 24-H-6/AH-2-3) as scion (ERU caused lower shoot growth than the control (N-efficient). It is also seen that well-developed strong root systems are the most important factor contributing to N use efficiency in pepper.
Key words: NUE-efficiency; inbred line; grafting; rootstock; root morphology
Corresponding author: Firdes Ulas, Department of Horticulture, Faculty of Agriculture, Erciyes University, Kayseri, Turkey. E-mail: fulas@erciyes.edu.tr, Tel:+90 537 919 3185
Published in „GENETIKA“ Vol. 53, No.3(2021), pp. 1273-1286
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-ZemunSerbia
UDC 575.630
https://doi.org/10.2298/GENSR2103273G
Original
scientific paper
GENETIC DIVERSITY AND POPULATION STRUCTURE ANALYSIS IN Lonicera L. (Caprifoliaceae) WITH THE USE OF ISSR MOLECULAR MARKERS
Shima GARSHASBI1, Alireza IRANBAKHSH*1, Yones ASRI2, Saeed Zaker BOSTANABAD3
1Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran
3Department of Microbiology and Biology, Parand branch, Islamic Azad University, Tehran, Iran
Abstract
Garshasbi S., A. Iranbakhsh, Y. Asri, S. Z. Bostanabad (2021). Genetic diversity and population structure analysis in Lonicera L. (Caprifoliaceae) with the use of ISSR molecular markers. - Genetika, Vol 53, No.3, 1273 - 1286.
Species delimitation is essential since species is regarded as the basic unit of analysis in nearly all biological disciplines, such as ecology, biogeography, conservation biology, and macroevolution. The genus Lonicera, which includes approximately 200 species, is a major component of the family Caprifoliaceae, comprising a large number of horticultural and economically important shrubs and tree species. This genus is represented in Iran by 9 species. In spite vast distribution of many Lonicera species that grow in Iran, there are not any available report on their genetic diversity, mode of divergence and patterns of dispersal. Therefore data molecular (ISSR markers) with four primer pairs generated 77 polymorphic bands.and morphological studies of 70 accessions from 7 species of Lonicera, that were collected from different habitats in Iran were performed. The aims of the present study are: 1) to find the diagnostic value of ISSR markers in delimitation of Lonicera species, 2) to find the genetic structure of these taxa in Iran, and 3) to investigate the .species inter-relationship. The present study revealed that combination of morphological and ISSR data can delimit the species. AMOVA and STRUCTURE analysis revealed that the species of Lonicera are genetically differentiated but have some degree of shared common alleles.
Key words: Inter-simple sequence repeat, Morphology, Species delimitation, Lonicera
Corresponding author: Alireza Iranbakhsh, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. E-mail: iranbakhshar@yahoo.com; nasar.nas1990@gmail.com
Published in „GENETIKA“ Vol. 53, No. 3(2021), pp. 1287-1296
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2103287X
Original
scientific paper
GENETIC DIVERSITY AND GENE-POOL OF Salicornia sinus-persica Akhani
BASED ON RETROTRANSPOSON-BASED MARKERS
Pengyuan XU1*, Chenheng XU2, Xuan HUANG3, Hua WANG4,
Hakimeh MORABBI HERAVI5
1Jiangsu Normal University, Xuzhou City, Jiangsu Province, China
2Tianjin University of Commerce, School of Economics, Tianjin, China
3Beijing Foreign Studies University, International Business School, Beijing, China
4Hebei University of Technology, School of Artificial Intelligence, Tianjin, China
5Department of Statistics, University of Bojnord, Iran
Abstract
Xu P.,C. Xu, X.Huang, H.Wang, H. Morabbi Heravi (2021). Genetic diversity and gene-pool of Salicornia sinus-persica akhani based on retrotransposon-based markers. - Genetika, Vol 53, No.3, 1287 - 1296.
Salicornia Sinus-Persica Akhani has been discovered in southern Iran, namely in Khuzestan, Bushehr, and Hormozgan, along salty and brackish rivers draining into the Persian Gulf well as estuaries and coastal habitats. Genetic diversity is an essential component of biological variability for conservation methods, particularly for rare and narrowly endemic species. Iran's folk's genetic structure, diversity, and morphological distinctions are unknown. Because of the plant's medicinal value, genetic diversity and folk structure were studied in six regional folks of Salicornia Sinus-Persica Akhani. To emphasize genetic variability within and across people in this plant, we employed six inter-retrotransposon amplified polymorphism (IRAP) markers and 15 combined IRAP markers. The AMOVA test indicated a substantial genetic difference across the folks investigated (PhiPT = 0.41, P = 0.010), as well as the fact that within-folk variation accounted for 80% of overall genetic diversity and genetic divergence between folks accounted for 20%. The Mantel test revealed a substantial positive connection between genetic distance and geographical distance across all groups investigated. According to STRUCTURE analysis and folk assignment tests, these folks exhibit some degree of gene flow—the UPGMA classification of molecular data matched with the PCoA plot of people. Based on (IRAP) markers, these findings revealed that the regional folks of Salicornia Sinus-Persica are incredibly distinct.
Key words: Gene flow, IRAP, Salicornia sinus-persica; Population Differentiation
Corresponding author: Pengyuan Xu, Jiangsu Normal University, Xuzhou City, Jiangsu Province, China, E-mail: sabahmaryam707@gmail.com; qq.com@3272464246
Published in „GENETIKA“ Vol. 53, No.3(2021), pp. 1297-1309
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2103297U
Original scientific paper
OPTIMISED FEATURE SELECTION FOR EARLY CANCER DETECTION
K.R.UTHAYAN1*, S.MOHANAVALLI1, B.NIVETHA2, S. DHIVYA1
1Department of Information technology, Sri Sivasubramaniya Nadar College of Engineering, India
2Goldman Sachs, Bangalore, India
Abstract
Uthayan K.R. S.Mohanavalli, B.Nivetha, S. Dhivya (2021). Optimised feature selection for early cancer detection. - Genetika, Vol 53, No.3, 1297-1309.
Global Cancer Incidence, Mortality and Prevalence (GLOBOCAN) status report for the year of 2020, suggests the occurrence of 10.0 million cancer deaths and 19.3 million new cancer cases. Clearly, cancer incidence and mortality are rapidly growing worldwide. Also, the leading causes of cancer deaths are found to be lung cancer and breast cancer. Cancer cells are having the probability of spreading to other parts of the body too. Most chronic cancers are not curable, but some can be controlled for a few months or years. Also, there is a possibility of high rate of relapse of the disease. These remissions can be partial or complete. But, if detected early, certain cancers can be treated by surgery, chemotherapy, and radiation therapy. This research work focuses on detecting cancer in its early stage so that right measures can be taken to combat the disease. In this attempt to create a beneficial working model, the combination of Artificial Neural Network (ANN), Convolution Neural Network, Graph based Neural Network with Genetic Algorithm (GA) have proven to be successful. As a proof of concept, we present a combination of feature selection techniques that can effectively reduce the feature set and optimize the classification techniques. The proposed method, when applied on a benchmark dataset, gave a higher accuracy by selecting most relevant 7 features out of 10 with an accuracy of 95.7%. Using Convolution Neural Network, the accuracy improved to 98.3% with optimal hyperparameter tuning.
Key words: Convolution Neural Network, Graph Neural Network, Genetic Algorithm, Logistic Regression, Support Vector Machine
Corresponding author: Uthayan K.R, Department of Information technology, Sri Sivasubramaniya Nadar College of Engineering, India, E-mail: uthayankr@ssn.edu
Published in „GENETIKA“ Vol. 53 No.3(2021), pp. 1311-1320
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.15
https://doi.org/10.2298/GENSR2103311N
Original
scientific paper
The occurrence of mycotoxins in sweet maize hybrids
Milica NIKOLIĆ*, Jelena SRDIĆ, Iva SAVIĆ, Slađana ŽILIĆ, Milan STEVANOVIĆ,
Vesna KANDIĆ, Slavica STANKOVIĆ
Maize Research Institute, Belgrade, Serbia
Abstract
Nikolić M., J. Srdić, I. Savić, S. Žilić, M. Stevanović, V. Kandić, S. Stanković (2021). The occurrence of mycotoxins in sweet maize hybrids. - Genetika, Vol 53, No.3, 1311-1320.
The objective of the present study was to investigate the susceptibility of maize hybrids to the natural mycotoxins contamination: aflatoxin total (AFLA), deoxynivalenol (DON), zearalenon (ZEA) and fumonisins (FB). Hybrids were grown during two production years (2019 and 2020) in two locations. Mycotoxin contamination of maize grains was evaluated in five sweet maize hybrids. Contamination level of investigated hybrids of first cluster was mainly by below average values of ZEA (all equal to zero) and DON. Hybrids PK4 (S) 2020, PK6 (MS) 2020 and PK4 (MS) 2020 had below average values for AFLA, while remaining treatments of this cluster have mostly elevated values of this mycotoxin. Contamination level of investigated hybrids of second cluster mostly had increased values of mycotoxins ZEA and DON and below average values of AFLA. Samples did not contain fumonisins. Mycotoxin contamination were significantly affected by hybrids and years. We have established that DON and ZEA levels were influenced by the environmental conditions. There were no significant effects of location on the level of AFLA in the sweet maize hybrids. The variation in the properties of mycotoxin content (DON) was significantly influenced by hybrids, and there was no significance of hybrids x location interaction. Differences were more expressed for the content of ZEA and AFLA compared to the content of DON. Hybrid PK1 had the lowest content of DON, while it had the highest content of ZEA. Mycotoxin analyses showed that in all tested hybrids, levels of AFLA, DON, ZEA and FBs were below the maximum permissible levels stipulated by the legislation of the European Union and the Republic of Serbia in maize intended for direct human consumption. These results confirmed that the susceptibility of hybrids is one of the important risks, in addition to climatic factors, for the appearance of toxigenic fungi and their mycotoxins. Genotype tolerance is very important as a preventive measure, which indicates that breeders have to pay attention to it in sweet maize breeding programs.
Key words: aflatoxins, deoxynivalenol, zearalenon, fumonisins
Corresponding author: Milica Nikolić, Maize Research Institute, Zemun Polje, Slobodana Bajića 1, 11185, Belgrade, Serbia, mnikolic@mrizp.rs
Published in „GENETIKA“ Vol. 53, No.3(2021), pp. 1321-1330
© 2021Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2103321M
Original scientific paper
INTER-SIMPLE SEQUENCE REPEATS GENETIC RELATIONSHIPS BETWEEN POPULATIONS OF Consolida (RANUNCULACEAE)
Kuanhong MENG 1*, Jia YAO2, CongYing HE1and Mohsen FARSHADFAR3
1Ningbo Institue of Oceanography Ningbo, Zhejiang, 315832, China
2School of Civil Engineering and Architecture, NingboTech University Ningbo, Zhejiang, 315100, China
3Department of Agriculture, Payame Noor University (PNU), Tehran, Iran
Abstract
Meng K., J. Yao, C. He, M. Farshadfar (2021). Inter-simple sequence repeats genetic relationships between populations of Consolida (Ranunculaceae). - Genetika, Vol 53, No.3, 1321-1330.
In fields like ecology, biogeography, conservation biology, and macroevolutionary theory, species are considered as the fundamental unit of study. Gray (1821), an expert on British flora, regarded the genus Consolida to be a distinct genus based on a single species (C. regalis). Consolida, on the other hand, is believed by some scholars to be a part of Delphinium. There are no reports on the genetic diversity, modes of divergence, or dispersion patterns of the various Consolida species that grow in Iran despite their widespread distribution. Hence, a combination of atomic (ISSR markers) and morphological examinations of 78 increases from seven species of Consolida were conducted. One goal of this work is to determine the diagnostic usefulness of ISSR markers in the delimitation of Consolida species, another is to determine the genetic structure of these taxa, and a third is to look at the relationships between species within these groups. It was discovered in the current research that combining morphological and ISSR data may help identify species. Analysis using AMOVA and STRUCTURE found that the species of Consolida are genetically distinct, yet have some common genes.
Key words: Consolida, Inter-simple sequence repeat, Morphology, Species delimitation
Corresponding author: Kuanhong Meng, Ningbo Institue of Oceanography Ningbo, Zhejiang, 315832, China, E-mail: guxing_ge@163.com; shahramfadat1@gmail.com
Published in „GENETIKA“Vol. 53, No.3(2021), pp. 1331-1340
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2103331L
Original
scientific paper
GENETIC DIVERSITY AND GENE-POOL OF Aegilops tauschii Coss. (Poaceae) BASED ON RETROTRANSPOSON-BASED MARKERS
Sha LI1*, Xiaomei JIANG2, Shahram MEHRI3
1College of Architecture, Chongqing Metropolitan College of Science and Technology, Yongchuan, Chongqing, 402167, China
2Chengdu Celec Engineering Co.,Ltd., Chengdu, Sichuan, 610000, China
3Department of Agronomy and Plant Breeding, ParsAbad Moghan Branch,
Islamic Azad University, ParsAbad Moghan, Iran
Abstract
Li S. X. Jiang, S. Mehri (2021). Genetic diversity and gene-pool of Aegilops tauschii coss. (Poaceae) based on retrotransposon-based markers. - Genetika, Vol 53, No.3, 1331-1340.
Genetic variation is an essential feature of biological variety for conservation methods, particularly for scarce and strictly endemic species. Iran's population genetic structure, genetic variation, and morphological variations are all unknown. Because of the therapeutic value of this species, six regional populations of Aegilops tauschii were studied for genetic diversity and population structure. To uncover within and among population genetic variation in this plant, we employed six inter-retrotransposon amplified polymorphism (IRAP) markers and 15 combined IRAP markers. The AMOVA test indicated a substantial genetic difference (PhiPT = 0.66, P = 0.010) among the tested populations, as well as the fact that 85 percent of overall genetic differences was related to within-population variety and 15 percent to genetic divergence across populations. The Mantel test revealed a substantial positive connection between genetic distance and geographical distance across the populations investigated. Based on (IRAP) markers, these findings suggested that regional populations of Aegilops tauschii are well separated.
Keywords: Gene flow, IRAP, Aegilops tauschii; population differentiation
Corresponding author: Sha Li, College of Architecture, Chongqing Metropolitan College of Science and Technology, Yongchuan, Chongqing, 402167, China, E-mail: forward0803@163.com; shahramfadat1@gmail.com
Published in „GENETIKA“Vol. 53, No3(2021), pp. 1341-1356
© 2021 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633
https://doi.org/10.2298/GENSR2103341J
Original scientific paper
PROTEIN QUALITY PARAMETERS AND STORAGE PROTEIN PROFILING
OF MUNGBEAN INTERSPECIFIC LINES (Vigna radiata L. Wilczek)
V. JAIN and S. SHARMA*
Department of Biochemistry, Punjab Agricultural University, Ludhiana, 141004, India
Abstract
Jain V. and S. Sharma (2021). Protein quality parameters and storage protein profiling of Mungbean interspecific lines (Vigna radiata L. wilczek). - Genetika, Vol 53, No.3, 1341-1356.
Present investigation reports the protein quality parameters in mungbean x ricebean (MR) and mungbean x urdbean (MU) interspecific lines along with mungbean checks. Interspecific lines contained 138.4-230.3 mg/g total soluble proteins, 0.84-2.63 mg/g free amino acids, 3.21-7.10 mg/g methionine, 0.08-0.15 mg/g cysteine and 0.67-1.32 mg/g tryptophan. Protein fractionation studies revealed 32.6-48.3 (albumins), 153.3-218.8 (globulins), 2.81-8.30 (prolamins) and 22.22-66.23 mg/g seed (glutelins). Among MU lines, mean albumin content was lower and globulins, prolamins and glutelins were significantly higher than that of mungbean checks. Globulins showed a positive correlation with total soluble proteins and tryptophan while albumins were negatively correlated to prolamins and positively correlated to methionine. High positive correlation was recorded between prolamins and glutelins, as well as prolamins and cysteine at 1% level of significance. Both prolamins and glutelins exhibited a negative correlation with methionine at P<0.05. Electrophoretic analysis of total proteins revealed presence of 6-11 bands in molecular weight ranging from 13-162 kDa. Cluster analysis of the resolved gel revealed 9 clusters at 95% homology with SML 2011, the most distant interspecific line among all genotypes. Overall, SML 1827 and SML 2033 were rich in total soluble proteins, free amino acids, methionine, tryptophan, albumins and globulins. The results suggest that electrophoretic profiles of mungbean interspecific lines provide valuable information with potential of being used in mungbean genetic improvement.
Keywords: Mungbean; Interspecific lines; Proteins; Electrophoresis; Cluster analysis.
Corresponding author: S. Sharma, Department of Biochemistry, Punjab Agricultural University, Ludhiana, 141004, India, E-mail: suchetasharma_pau@pau.edu, +91-9888412565
Published in „GENETIKA“Vol. 53, No.3(2021), pp.1357-1368
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2103357Z
Original scientific paper
SPECIES IDENTIFICATION AND POPULATION STRUCTURE ANALYSIS
IN Hesperis L. (Brassicaceae)
Zhiwei ZHANG1,*, Hangcheng YU2, Shuowen FENG1, Amir Abbas MINAEIFAR3
1School of Architecture and Civil Engineering, Chongqing Metropolitan College of Science
and Technology, Yongchuan,Chongqing, 402167, China
2College of Forestry,Guizhou University,Guiyang,Guizhou,550000,China
3Department of Biology. Payame Noor University. P.O. Box19395-3697 Tehran, Iran
Abstract
Zhang Z., H. Yu, S. Feng, A. A. Minaeifar (2021). Species identification and population structure analysis in Hesperis L. (Brassicaceae). - Genetika, Vol 53, No.3, 1357 - 1368.
In biology, biogeography, ecology, and conservation, species identification is critical. Hesperis L. is a Brassicaceae (Cruciferae) genus of 46 species found across the temperate northern hemisphere, from South and Central Europe to Southwest Asia, Caucasia, and the mountainous portions of West China and Mongolia. According to the latest therapies, Hesperis is expressed in Iran by six species. Despite the fact that several Hesperis species are widely distributed throughout Iran, nothing is known about their genetic diversity, divergence techniques, or dispersion patterns. As a result, we examined 122 accessions from six Hesperis species gathered from various locations in Iran using genetic (ISSR markers) and morphological techniques. A total of ten ISSR markers were employed in this study. The Nei's genetic distance was used to measure genetic distances, and descriptive statistics of folks were used to produce genetic parameters. There were a total of 118 polymorphic bands found. The study's aims are as follows: 1) Is it possible to identify Hesperis species using ISSR markers? 2) In Iran, what is the genetic make-up of these taxa? 3) How do interspecies relationships work? According to the conclusions of this research, morphological and ISSR data may be utilized to identify species. The Hesperis species are genetically distinct yet share significant genes, according to the AMOVA and STRUCTURE analyses.
Keywords: ISSR, morphology, species identification, STRUCTURE analyses
Corresponding author: Zhiwei Zhang, School of Architecture and Civil Engineering, Chongqing Metropolitan College of Science and Technology, Yongchuan,Chongqing, 402167, China. E-mail:nasar.nas1990@gmail.com; xiaowei988211@163.com
Published in „GENETIKA“Vol. 53, No.3(2021), pp.1369-1377
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2103369L
Original scientific paper
GENETIC DIVERSITY IN Stellaria L. (Caryophyllaceae) USING SEQUENCE RELATED AMPLIFIED POLYMORPHISM
Jialing LI1 , Xin YANG2,b*, Shahram MEHRI3
1College of Engineering Management, Nueva Ecija University of Science and Technology, Cabanatuan, Philippines
2College of Bioengineering, Chongqing University, Chongqing 400030, China
3Department of Agronomy and Plant Breeding, ParsAbad Moghan Branch,
Islamic Azad University, ParsAbad Moghan, Iran
Abstract
Li J., X. Yang, S.Mehri (2021). Genetic diversity in Stellaria L. (Caryophyllaceae) using sequence related amplified polymorphism. - Genetika, Vol 53, No.3, 1369 - 1377.
Nearly 120 species exist in the genus Stellaria L. (Caryophyllaceae, Alsinoideae) with a general distribution in the temperate zones of Asia and Europe. Totally, Stellaria exhibits 9 taxa in Iran and sequence-related amplified polymorphism was used to assess their genetic diversity. Therefore, 72 cases of 5 Stellaria were collected in seven provinces. Then, polymerase chain reaction amplifications (PCR) amplification of 5 species of Stellaria was employed for producing 78 (Number of total loci) (NTL) DNA bands. In addition, five selective primers were combined to produce the above bands. Results showed the total number of amplified fragments in ranges between 8 and 15 and variations of the predicted unbiased heterozygosity (H) between 0.22 (S. persica) and 0.39 (S. holostea). Moreover, estimations indicated the genetic similarity between 5 species from 0.73-0.92 and two key clusters were obtained by the clustering results. Considering the analysis of the SRAP markers, the minimum similarity was observed in S. pallida and S. holostea. Furthermore, distance (Mantel test results) showed a considerable signature of isolation. According to the findings, SRAP can detect and decipher genetic affinities in the Stellaria species. Hence, these findings could be used in conservation and biodiversity programs and also provide the ground for choosing appropriate ecotypes for pasture and forage purposes in Iran in the future.
Key words: Gene Flow, Genetic Diversity, Sequence-related amplified polymorphism, Stellaria.
Corresponding author: Xin Yang, College of Bioengineering, Chongqing University, Chongqing 400030, China, E-mail: yaaangx@126.com; esfandiarim80@gmail.com
Published in „GENETIKA“Vol. 53, No.3(2021), pp.1379-1386
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2103379A
Original scientific paper
X CHROMOSOMAL ANALYSIS IN POPULATION GENETICS AND FORENSIC SCIENCE: A MINI REVIEW
Azam ALI1,2*, Nazim HUSSAIN2, Muhammad Saqib SHAHZAD3, INAMULLAH2,
Qurban ALI2*
1Institute of Molecular Biology & Biotechnology (IMBB), the University of Lahore, Lahore, Pakistan
2Centre of Applied Molecular Biology (CAMB), University of the Punjab, Lahore, Pakistan
3Forensic Sciences Department, University of Health Sciences, Khayaban-e-Jamia Punjab, Lahore, Pakistan
Abstract
Ali A., N. Hussain, M. S. Shahzad, Inamullah, Q. Ali (2021). X chromosomal analysis in population genetics and forensic science: a mini review- Genetika, Vol 53, No.3, 1379-1386.
The human X chromosome analysis has been applied to decipher the genetic structure of populations for applications in medical genetics and for human identification, parentage analysis and kinship analysis. Although it has not been studied on vast level with regard to human populations with comparison to other of its counterparts like autosomal markers, Y chromosome and mtDNA yet it is important for great potential in studying oncology, various diseases and forensic science applications. In this mini review, a snapshot of X chromosomal properties as genetic marker has been entailed. The structure and potential multiplex oriented kits utilizing X chromosomal markers have been discussed. Moreover, concerns of different researchers over X chromosomal published data have been referred to point out need of analyzing X chromosomal markers to unravel their role in population genetics, medical genetics and human identification.
Keywords: X chromosome, genetic markers, kinship, forensic
Corresponding author: Azam Ali, Institute of Molecular Biology & Biotechnology (IMBB), the University of Lahore, Lahore, Pakistan, E-mail: azam.skyblue@gmail.com
Published in „GENETIKA“Vol. 53, No.3(2021), pp.1387-1388
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
https://doi.org/10.2298/GENSR2103387E
CORRIGENDUM
by
Snežana Mladenović Drinić, Editor of the journal Genetika
request from authors to replace Fig 3. Electrophoresis gel of studied ecotypes from DNA fragments produced by ISSR in paper
GENETIC DIVERSITY AND RELATIONSHIPS AMONG SALVIA SPECIES
BY ISSR MARKERS
by
Zhao YANPENG1, Wang HONGMEI2*, Liang WEI3, Majid KHAYATNEZHAD4, FAISAL5
1Department of Fundamental Courses, Shandong University of Science and Technology, Taian, Shandong, China
2Dept. of Physical Education, University of Science and Technology Liaoning, Anshan, China
3Institute of Physical Education, Qingdao University of Science and Technology, Qingdao Shandong 266061, China
4Department of Environmental Sciences and Engineering, Ardabil Branch, Islamic Azad University, Ardabil, Iran
5 Institute of Plant Sciences, University of Sindh, Jamshoro, Pakistan
Original scientific paper
https://doi.org/10.2298/GENSR2102559Y
published in the journal Genetika, 2021, Vol 53, No.2, 559 -574
Published in „GENETIKA“Vol. 53, No.3(2021), pp1389-1390
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
https://doi.org/10.2298/GENSR2103389E
CORRIGENDUM
by
Snežana
Mladenović Drinić, Editor of the journal Genetika
request
from authors to replace Figure 4. Gel
Electrophoresis image of DNA fragments of Geranium species. in paper
DIVERSITY IN Geranium (Geraniaceae) USING RAPD MARKER
by
Juan YIN1*, Majid KHAYATNEZHAD2, Abdul SHAKOOR3,4
1Forestry College, Xinyang College of Agriculture and Forestry, Xinyang, Henan, 464000, China
2Department of Environmental Sciences and Engineering, Ardabil Branch, Islamic Azad University, Ardabil, Iran
3College of Environment and Planning, Henan University, Kaifeng, 475004, Henan, China
4Key Laboratory of Geospatial Technology for the Middle and Lower Yellow River Regions, Ministry of Education, Kaifeng 475004, Henan, China
Original scientific paper
https://doi.org/10.2298/GENSR2101363Y
published in the journal Genetika, 2021, Vol 53, No.1, 363 -378
Published in „GENETIKA“Vol. 53, No.3(2021), pp1391-1392
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
https://doi.org/10.2298/GENSR2103391E
CORRIGENDUM
by
Snežana Mladenović Drinić, Editor of the journal Genetika
Corresponding author request to replace Figure 3. in manuscript
STUDY ON GENETIC DIVERSITY BETWEEN Malva L. (Malvaceae): A HIGH VALUE MEDICINAL PLANT USING SCOT MOLECULAR MARKERS
by
Huixing LI1*,Haiyan YU1, Xiancai ZENG1, Sahar HUSSEIN HAMARASHID2
1School of Biological and Chemical Engineering, Nanyang Institute of Technology, Nanyang 473004, Henan, China
2Sulaimany polytechnic University - College of applied science
https://doi.org/10.2298/GENSR2102895L
published in the journal Genetika, 2021, Vol 53, No.2, 895-910
Published in „GENETIKA“Vol. 53, No.3(2021), pp1393
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
https://doi.org/10.2298/GENSR2103393E
CORRIGENDUM
by
Snežana Mladenović Drinić, Editor of the journal Genetika
request from Abdul Waheed, Department of Plant Science, Quaid-i-Azam University, Islamabad, Pakistan – 45320, Email: awaheed@bs.qau.edu.pk to remove his name from the manuscript
ASSESSMENT OF GENETIC STRUCTURE AND DIVERSITY OF Erodium (Geranaiceae) SPECIES
by
Lejing LIN1* , Li LIN1, Abdul WAHEED2
1College of Landscape and EcologyNingbo City College of Vocational Technology, Ningbo Zhejiang, 315100, China
2Department of Plant Sciences, Quaid-i-Azam University Islamabad, Pakistan
https://doi.org/10.2298/GENSR2102507L
published in the journal Genetika, 2021, Vol 53, No.2, 507-520
Published in „GENETIKA“Vol. 53, No.3(2021), pp1395-1396
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
https://doi.org/10.2298/GENSR2103395E
CORRIGENDUM
by
Snežana Mladenović Drinić, Editor of the journal Genetika
request to replace section Discussion in paper
EXPRESSION OF CAVEOLIN-2 IN PATIENTS WITH ORAL CANCER AND CORRELATIONS WITH CLINICOPATHOLOGICAL PARAMETERS
by
Chao WAN, Fang ZHANG, Liangming ZHU*
Department of Oral and Maxillofacial Surgery, Yijishan Hospital of Wannan Medical College, Wuhu 241000, Anhui Province, P. R. China
https://doi.org/10.2298/GENSR2102703W
published in the journal Genetika, 2021, Vol 53, No.2, 703-716
due to technical error this part belong to another paper about Pistachio.